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0 votes
1 reply
483 views
Multiplex Primer Design to Distinguish 7 Bacterial Genomes
primer qpcr microbiome
updated by colindaven 844 • | written by dirtees 0
0 votes
0 replies
311 views
Forum: What’s the minimum evidence you’d need to trust an AI triage tool saves time without missing P/LP calls?
software ngs bioinformatician pipeline
written by sameer 4
0 votes
0 replies
359 views
Query regarding GTEx sample ID structure, histology matching, and new tissue sites in GTEx v11
rna-seq gtex
written by mukherjeeliana24 0
0 votes
0 replies
537 views
Best practice for filtering VEP-annotated variants for Protein Language Model training (EVE/AlphaMissense/ProtBert/ESM): Canonical vs. All Isoforms?
variantfiltering genomics vep gnomad
written by Mustafa 0
0 votes
1 reply
562 views
Problem loading 10X RNA-seq data in R: length of Dimnames[[1]] mismatch
read10x seurat rnaseq r
updated by Wayne 208 • | written by samuelwatson0711 0
0 votes
3 replies
817 views
makeTxDbFromBiomart cannot open URL
database txdb biomart ensembl
updated by GenoMax 16K • | written by npont 3
0 votes
1 reply
1.6K views
News: Introduction to Deep Learning for Biologists - online course, 3–7 November
machinelearning python deeplearning convolutionalneuralnetwork
written by Physalia-courses 281
0 votes
0 replies
292 views
Need help with jagged plotProfile results in deepTools (LACE-seq data)
deeptools
written by zhuzhuzhu 0
0 votes
0 replies
416 views
Tool: PROTAC-Linker-Pathfinder: Automated exit vector identification for undruggable targets (Python/Monte Carlo)
protac python drug-design structural-biology tool
written by BioMed_Student 0
0 votes
7 replies
1.2K views
Tool: VATAligner for Beta testing
software alignment homology tgs ngs
updated by ATpoint 9.1K • | written by xuan13hao 0
0 votes
1 reply
420 views
Improve predictive models and validation based on ROC
validation predictive-models roc rnaseq
updated by Ram 4.5K • | written by n@4j 0
0 votes
2 replies
653 views
Trimmomatic
rna-seq quality-control fastq trimmomatic galaxy
updated by Istvan Albert 10K • | written by Mahsa80.mm 0
0 votes
0 replies
239 views
Partititon whole mitogenomes from different genera for BEAST analysis
partitionfinder partition beast annotation mitogenomes
written by Álvaro 0
0 votes
0 replies
340 views
News: Spring School in Bioinformatics - ONLINE, 8-12 JUNE
genomeassembly docker rnaseq phylogenomics ngs
written by Physalia-courses 281
0 votes
1 reply
554 views
MuSE calls too few snps
muse snp
updated by Ram 4.5K • | written by yalin 0
0 votes
6 replies
1.2K views
Best pipeline to merge multiple consumer raw DNA files into PLINK + EIGENSTRAT (1240K) with minimal SNP loss?
dna
written by Persona 0
0 votes
5 replies
1.1K views
RNA-seq (bacteria): two control replicates look TR-like (PCA + marker genes).
exclude pca rnaseq limma outlier
updated by Dunois 301 • | written by MaxMin 1
0 votes
3 replies
794 views
dbGAP control data access download
dbgap
updated by Ram 4.5K • | written by shrivastav.k.abhi 0
0 votes
25 replies
3.8K views
-taxids option in blast is not working
blast
written by caroline.zanchi 0
0 votes
0 replies
256 views
MaGECK MLE Error: TypeError: unsupported operand type(s) for +: 'float' and 'list'
mageck python cripsr
updated by GenoMax 16K • | written by jamie3355 0
0 votes
0 replies
285 views
selscan XPEHH map file
selscan mapfile
updated by Ram 4.5K • | written by SeoG 1
0 votes
3 replies
701 views
May I use Seurat package to analyze single cell RT-qPCR datasets generated on Biomark FluidigmN
seurat biomark pcr fluidigm single-cell
updated by ATpoint 9.1K • | written by francois-xavier.mauvais 0
1 vote
1 reply
604 views
News: Last call: In-person NGS data analysis & variant calling workshop (Munich, Feb 24–26, 2026)
mapping dnaseq rnaseq illumina variantcalling
written by ecSeq Bioinformatics 9
0 votes
1 reply
370 views
Positive QMEAN Z-scoring
qmean.
updated by Mensur Dlakic 3.0K • | written by Wided 0
0 votes
0 replies
390 views
Job: Postdoctoral Fellow in Computational Human Epigenetics | Oslo, Norway
epigenetics bioinformatics epidemiology
written by Arup Ghosh 402
0 votes
8 replies
1.5K views
find and count motif in FASTA file
grep python seqtk
updated by lieven.sterck 1.6K • | written by Matteo Ungaro 16
6 votes
14 replies
9.6K views
Forum: The ethics of sci-hub
paywall open-science journals publications
updated by Garry F. Goods 0 • | written by WouterDeCoster 4.8K
0 votes
2 replies
547 views
Transfer gene ontology annotations
go-term annotation gene-ontology
updated by Ram 4.5K • | written by ja569116 0
1 vote
0 replies
636 views
Job: PhD (Doctoral) position - Data Sciences for Molecular Systems Biology & Bioinformatics
bioinformatics biomedicine phd data-science r
written by a_gruber 5
0 votes
5 replies
950 views
BED File from COSMIC Mutation
cosmic
updated by michael.ante 408 • | written by anasjamshed 14
0 votes
4 replies
795 views
hisat2 error
alignment hisat2
updated by Arup Ghosh 402 • | written by aj123 14
0 votes
0 replies
309 views
Setting Thresholds and Interpreting Results in GWAS
gwas rna-seq
written by CaERu _no_ korokoro 0
0 votes
4 replies
1.0K views
hifiasm de novo assembly produces short contigs that translate to chromosomes longer than reference
hifiasm ngs denovo
updated by Ram 4.5K • | written by ferran.llobet 2
0 votes
2 replies
659 views
Interpretation of DEG Analysis Results
deseq2 deg
updated by GenoMax 16K • | written by CaERu _no_ korokoro 0
0 votes
2 replies
859 views
PCA, Pearson correlation
pca chipseq
updated by Ram 4.5K • | written by Irving 0
0 votes
3 replies
1.1K views
~80% duplicate pairs in RNAseq data
rna-seq
updated by GenoMax 16K • | written by Irvin 0
0 votes
4 replies
1.4K views
How to choose BLAST identity and coverage thresholds without excluding true homologs?
metagenomics
updated by Mensur Dlakic 3.0K • | written by Sachin 2
0 votes
5 replies
1.1K views
Gene coverage in Genome Analysis
coverage ensembl genome
written by npont 3
0 votes
0 replies
411 views
News: Machine Learning with R & tidymodels (16–20 Feb)
machinelearning omics genomics ai r
written by Physalia-courses 281
0 votes
2 replies
662 views
Any method to extract k-mer seqs of a specific taxid from KrakenUniq/Kraken database?
krakenuniq kraken
updated by DareDevil 448 • | written by Depan 0
0 votes
4 replies
1.2K views
Multi/Cross Species rnaseq analysis
deseq2 rnaseq salmon
updated by dthorbur 350 • | written by Tom Brady 0
0 votes
1 reply
2.0K views
I performed Homer's annotatePeaks.pl on multiple tissues, yet when I run annotatePeaks on the merged tissues, I get fewer total annotated peaks. Is there an explanation for this?
homer atacseq
updated by Surbhi 1 • | written by Ronin 1
0 votes
5 replies
1.1K views
Pre-processing duplicates in SMARTer-seq data
rna-seq umi fastq smarter-seq fastp
updated by ATpoint 9.1K • | written by kiki 2
0 votes
1 reply
465 views
Integrating multiple GEO methylation datasets: at which step of preprocessing?
methylation infinium 450k
updated by Arup Ghosh 402 • | written by Ricardo 0
0 votes
1 reply
638 views
Truthset NA12878 T2T/Pangenome for benchmarking?
t2t benchmarking pangenome vcf alignment
updated by Arup Ghosh 402 • | written by smokestack 2
2 votes
0 replies
338 views
Tool: Ligase: Create multi-tool Docker images instantly (no Dockerfile required)
biocontainers software docker reproducibility pipeline
written by matthew 2
0 votes
10 replies
1.9K views
Dealing with unclassified metagenomic reads
metagenome
updated by Mensur Dlakic 3.0K • | written by arshad1292 11
1 vote
1 reply
531 views
How to quickly make a surjection between Minigraph graph and MC graph in HPRC
vg
updated by colindaven 844 • | written by gulin 1
0 votes
5 replies
1.1K views
Whole genome sequencing, different sequencing platforms, different quality control?
wgs snp bacteria
updated by SomeOne 33 • | written by yesquokkan 1
0 votes
1 reply
1.5K views
SNPduo error reading ped files
ibs genome snpduo ped
updated by muriel.grosb 0 • | written by amitgourav.ghosh12 7
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