Thank you for your kind answers.
I am focusing on sequence from a particular species, and comparing between strains where they have genomic variants that may change their phenotype.
I have some additional questions about standard Illumina QC.
I understand that the Illumina sequencers share the basic technology, but the average read lengths differ, ranging from 75bp to 300bp.
Then, should I set different minimum length? Because 50bp from the sample of average read length 75bp and 300bp should imply different meanings. Previous sample wouldn't be a big problem, but the latter one would mean severe quality problem, doesn't it?