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0 votes
0 replies
704 views
RSEM vs. SQUIRE for Transposable Element Accommodation
rna-seq
written by Etornam09 • Etornam09
0 votes
2 replies
782 views
Understanding RNAseq normalization
rnaseq normalization edger tximport
updated by ATpoint ATpoint • written by bioinfoguy2 • bioinfoguy2
0 votes
2 replies
967 views
How to identify ortholog sequences in non-model organisms not present on NCBI/UniProt?
ncbi orthologs
written by gikeda • gikeda
0 votes
0 replies
706 views
News: Single-Cell RNA-Seq Data Analysis – in-person workshop (March 23–25, 2026, Berlin, Germany)
singlecell 10x seurat rnaseq cellranger
written by ecSeq Bioinformatics • ecSeq Bioinformatics
0 votes
0 replies
597 views
News: Orchestrating Microbiome Analysis with Bioconductor - online course
r bioconductor microbiome metagenomics multiomics
written by Physalia-courses Physalia-courses
0 votes
4 replies
923 views
Merging RNA modifications with transcript features.
features transcript modifications bedtools rna
written by Samali • Samali
0 votes
0 replies
322 views
Trying to download the Human CpG data from X-inactive and X-active chr
cpg x-chromosome methylation
written by AMARU • AMARU
2 votes
0 replies
480 views
Tool: pylifemap: Mapping Data onto the Tree of Life
bioinformatics visualization metagenomics taxonomy
written by Damien • Damien
1 vote
0 replies
321 views
Tool: simd-accelerated DNA encoding library
dna simd encoding simdna
written by milesforjazz • milesforjazz
0 votes
3 replies
1.1K views
SNPs calling using long-read whole-genome sequencing
long-reads snps pacbio
updated by GenoMax GenoMax • written by YocelynGG • YocelynGG
6 votes
0 replies
559 views
Herald: The Biostar Herald for Wednesday, December 17, 2025
herald
written by Biostar Biostar
3 votes
0 replies
478 views
Tool: RAPTOR: RNA-seq pipeline optimizer with ML recommendations
rna-seq
written by ayehgeek • ayehgeek
0 votes
4 replies
1.4K views
Using DecontX (ambient RNA correction) with doublet detection on scRNA-seq
detection doublet ambient rna scrna-seq
updated by Bastien Hervé Bastien Hervé • written by carolofharvest • carolofharvest
0 votes
4 replies
925 views
Common motif sequence from HOMER, MEME
homer meme
updated by i.sudbery i.sudbery • written by Bio • Bio
0 votes
4 replies
1.3K views
How to Annotate Gene names in *.bedmethyl files generated from ModKit?
genomics nanopore multiomics modkit metagenomics
written by Vaishnav • Vaishnav
0 votes
5 replies
1.2K views
Building Salmon and STAR index using rat GRCr8 reference genome
star index salmon grcr8
written by Ajina • Ajina
0 votes
7 replies
1.6K views
Alternatives to Cellbender / barcode selection tools foor poor quality single NUCLEI samples?
nuclei single scrnaseq cellbender
updated by Bastien Hervé Bastien Hervé • written by txema.heredia • txema.heredia
0 votes
1 reply
1.6K views
Getting intensities using apt-genotype-axiom
genotype assembly pmra affymetrix
updated by s.w.vanderlaan • s.w.vanderlaan • written by Genotepes Genotepes
0 votes
2 replies
1.1K views
News: Prefer in-person over online? 2026 NGS workshops in Leipzig, Berlin & Munich (NGS, single-cell, epigenomics)
singlecell rnaseq dnaseq epigenomics nextflow
written by ecSeq Bioinformatics • ecSeq Bioinformatics
0 votes
1 reply
2.0K views
Different alternatives for downloading GWAS summary statistics en masse
gwas
updated by GrandmaFunk • GrandmaFunk • written by LauferVA LauferVA
1 vote
3 replies
1.1K views
Job: Bioinformatics Specialist (Infrastructure Focus)
infrastructure nextflow pipelines
written by Madelaine Gogol Madelaine Gogol
8 votes
0 replies
535 views
Tool: FastDup: A scalable, high-performance duplicate marking tool (20x faster than Picard with identical results)
marking-duplicate genome-analysis
written by YeWen • YeWen
0 votes
6 replies
1.6K views
Running Purge_dups on Hifiasm assemblies.
genome assemblies purge_dups
updated by shelkmike shelkmike • written by SomeOne • SomeOne
0 votes
0 replies
295 views
Identifying sequence motifs associated with base editing efficiency and mis-editing
base-editing
written by QX • QX
1 vote
1 reply
970 views
GDCprepare() fails for TCGA-LAML: Error in dplyr::select - Column `disease_response` doesn't exist
tcgabiolinks bioconductor tcga r
updated by shahab • shahab • written by amirnavidinia2014 • amirnavidinia2014
0 votes
5 replies
1.2K views
Expected behavior of Salmon with --numGibbsSamples 100?
bootstrap gibbs sampling salmon edger
updated by Gordon Smyth Gordon Smyth • written by Tonya S. • Tonya S.
0 votes
2 replies
670 views
Separating stubborn fibroblasts from epithelial cells in primary lung cancer cultures
primary-cell-culture epithelial-cells cell-separation fibroblast
updated by ATpoint ATpoint • written by Havva • Havva
0 votes
0 replies
568 views
News: Assembly and Annotation of Genomes course - online, 9-13 March
pacbio genomeassembly annotation nanopore
written by Physalia-courses Physalia-courses
1 vote
1 reply
971 views
Forum: A Call for Transparency in Citation Practices in the 2018 Galaxy Update (doi: 10.1093/nar/gky379)
citation self galaxy bioinformatics
updated by ATpoint ATpoint • written by Free • Free
0 votes
4 replies
1.4K views
RNAseq results from HEK293T cells can or cannot be used for Central Nervous System?
biology rnaseq transcriptomics
written by R.L. • R.L.
1 vote
4 replies
1.3K views
Building a custom genomic database
annotation database genome
updated by 5heikki 5heikki • written by anikcropscience • anikcropscience
2 votes
8 replies
1.6K views
Forum: Interest in an on-premise automated pipeline for proprietary vendor sequencing data (e.g. Illumina)?
dragen illumina automation pipeline
updated by Jeremy Leipzig Jeremy Leipzig • written by Matvii Mykhailichenko • Matvii Mykhailichenko
0 votes
5 replies
1.4K views
Should use Ambient RNA detection software for filtered_bc_matrices from Cell Ranger for snRNAseq
ambient snrnaseq 10x cellranger
updated by carolofharvest • carolofharvest • written by biotrekker • biotrekker
0 votes
0 replies
485 views
Job: Two internships in Nantes/France (In French)
nantes france internships
written by Pierre Lindenbaum Pierre Lindenbaum
1 vote
2 replies
591 views
Nextflow process fails with “Invalid method invocation call” despite correct tuple
nextflow frip
written by DdogBoss • DdogBoss
0 votes
2 replies
697 views
PASA- Structural annotation of genome assembly
pasa
updated by colindaven colindaven • written by rachana • rachana
0 votes
1 reply
502 views
Is there any way to download the table of predicted targets from miRDB?
mirdb
updated by GenoMax GenoMax • written by jhonatan.henrique • jhonatan.henrique
0 votes
0 replies
376 views
Statistical test to look for peak differentiation between two manhattan plots
statistics glm popgen r manhattan
written by jfaberha • jfaberha
4 votes
2 replies
999 views
Herald: The Biostar Herald for Tuesday, November 11, 2025
herald
updated by GenoMax GenoMax • written by Biostar Biostar
0 votes
1 reply
692 views
Tutorial: AI tutor for iCn3D to provide step-by-step instructions on how to view and analyze 3D structures
tutorial structure icn3d ai
written by Jiyao Wang • Jiyao Wang
0 votes
1 reply
449 views
What does PolyPhen-2 error mean?
polyphen-2
updated by Ram Ram • written by Jl • Jl
0 votes
2 replies
1.2K views
ChIPSeq Analysis on GEO Fastq data on Galaxy
chip-seq geo
updated by gynecoloji • gynecoloji • written by mjdm • mjdm
0 votes
0 replies
280 views
Hisat2 disable spliced alignment or not for DNA shotgun reads??
hisat2 spliced
written by Jon • Jon
0 votes
4 replies
895 views
scRNAseq annotation
annotation immune scrnaseq tumor
updated by bioadam • bioadam • written by mrashad mrashad
0 votes
1 reply
815 views
Trouble getting clean alignment and searching for sequences of interest
sequence blast phylogenetics alignment protein
updated by GenoMax GenoMax • written by rodeorogue • rodeorogue
0 votes
0 replies
704 views
WGCNA - module genes change with different input order of genes in expression data, but all other values are identical
co-expression analysis wgcna network
written by soy-cat • soy-cat
0 votes
0 replies
697 views
News: Online Course: Machine Learning for Multi-Omics Integration
data-integration bigdata multi-omics depp-learning machine-learning
written by Physalia-courses Physalia-courses
0 votes
0 replies
348 views
Differences in start/end coordinates in mothur
mothur 16s edna
written by mainly.microbe2 • mainly.microbe2
0 votes
0 replies
348 views
News: Unlock Biological Insights for Bioengineering using QIAGEN IPA
omicsdata pathwayanalysis bioengineeringresearch
updated by GenoMax GenoMax • written by Nicole • Nicole
0 votes
8 replies
2.7K views
How do I convert FASTQ files to VCF files?
gatk samtools vcf
written by CaERu _no_ korokoro • CaERu _no_ korokoro
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