Variant calling RADseq and WGS population genomic datasets
Does anyone have any guidance or suggestions for variant calling RADseq and WGS datasets to generate a single population genomic dataset?
Both datasets represent population-level samples for the same species. Our current workflow uses a highly complete/contiguous reference genome and BWA to call variants. However, even after stringently filtering the variants for depth and missing data neighbor-joining trees and other population genetic analyses show significant genetic distance between the WGS and RADseq datasets (see image).
Neighbor joining tree generated using bitwise distances:
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