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0 votes
1 reply
700 views
wf-trio (EPI2ME Desktop) – aggregate_pileup_gvcf failing with exit status 137 despite high-memory system
wf-trio
updated by Pierre Lindenbaum Pierre Lindenbaum • written by Yash Sinha • Yash Sinha
2 votes
11 replies
3.0K views
Ensembl genome browser is not working
genome browser ensembl
updated by Ensembl Outreach • Ensembl Outreach • written by funky_roller • funky_roller
0 votes
0 replies
286 views
News: Introduction to Python Programming for Biologists course - online, 9-12 February
python ngs programming
written by Physalia-courses Physalia-courses
0 votes
3 replies
593 views
low FeatureCounts in PBMC data
featurecounts rnaseq
updated by ATpoint ATpoint • written by Jacek • Jacek
1 vote
0 replies
364 views
Quality control of shotgun metagenomics data
network microbiome
written by yesquokkan • yesquokkan
0 votes
1 reply
504 views
MSTRG. gene id in differentially expressed gene list!
deseq2 stringtie mstrg
updated by GenoMax GenoMax • written by khanald2114 • khanald2114
0 votes
3 replies
763 views
Forum: Advice on spatial transcriptomics tool
spatial-transcriptomics
updated by ATpoint ATpoint • written by biofibxen123 • biofibxen123
0 votes
5 replies
1.2K views
Circos plot for contig–contig links supported by PacBio read alignments
pacbio circos
updated by Ram Ram • written by matvergara19 • matvergara19
0 votes
7 replies
1.8K views
Python in bioinformatics
python
written by ogunjinmirashidat • ogunjinmirashidat
0 votes
5 replies
1.1K views
Public data sets
datasets r
updated by Ram Ram • written by himanshu0102 • himanshu0102
1 vote
15 replies
4.2K views
Forum: Reuse of publicly available datasets
publication rna-seq sequencing
updated by Bourumir • Bourumir • written by sanathoi • sanathoi
0 votes
0 replies
330 views
News: Machine Learning for Multi-Omics Integration - online, 2-4 February
deeplearning machinelearning multiomics omics
written by Physalia-courses Physalia-courses
2 votes
4 replies
958 views
Can a dot (.) be used as the ALT field for a deletion in a VCF file?
vcf deletion ngs
written by terdon terdon
0 votes
2 replies
725 views
GATK CalculateContamination (paired mode) - NaN value
gatk calculatecontamination mutect2
written by Livia • Livia
0 votes
3 replies
765 views
non human homology detection
homology uniprot id
updated by GenoMax GenoMax • written by zizigolu zizigolu
0 votes
1 reply
510 views
StringTie to DESeq2 workflow
stringtie deseq2 rna-seq
updated by i.sudbery i.sudbery • written by khanald2114 • khanald2114
0 votes
5 replies
1.1K views
PacBio HiFi alignment: am I doing this right?
assembly pacbio
updated by Ram Ram • written by matvergara19 • matvergara19
1 vote
8 replies
13K views
No result in enrichGO
r clusterprofiler enrichgo
updated by GenoMax GenoMax • written by sugus sugus
0 votes
2 replies
779 views
Using delly to genotype a particular deletion
deletion delly genotyping
updated by trausch trausch • written by arsala521 • arsala521
0 votes
1 reply
916 views
Does it make sense to add together the concentrations of different stereospecifically numbered lipids?
metabolomics lipidomics lipids mass-spectrometry
updated by Ram Ram • written by Katia • Katia
0 votes
3 replies
1.2K views
How to combine vector fields with Waddington landscapes (utilizing the directional information of RNA velocity for weak constraints)
landscapes cell-fate waddington vector-fields rna-velocity
updated by Ram Ram • written by 167966742 • 167966742
1 vote
6 replies
1.1K views
Tool: MirAge - A novel Matrix Analyzer for fast DNA classification
dna analysing tool
written by Maarten Pater • Maarten Pater
1 vote
2 replies
627 views
Clarification on counting in featureCounts
featurecounts
written by Lc • Lc
0 votes
1 reply
1.0K views
Runtime expectations and alternatives for vsearch --usearch_global on large nucleotide FASTA datasets
ngs performance vsearch sequence-alignment immune-repertoire
updated by dthorbur dthorbur • written by emmzee • emmzee
0 votes
1 reply
634 views
Custom background gene set for enrichment in variant-based analysis?
clusterprofiler enrichment pathway analysis
updated by dthorbur dthorbur • written by iamsmor • iamsmor
5 votes
5 replies
2.7K views
Forum: Forum:What Are The Most Influential Bioinformatics/Computational Biology Papers Of 2025?
bioinformatics genomics survey snps papers
updated by colindaven colindaven • written by Raony Guimarães Raony Guimarães
1 vote
0 replies
494 views
News: Starting the New Year by building the foundations of Data Analysis!
r python statistics programming
written by Physalia-courses Physalia-courses
0 votes
0 replies
305 views
Cell-type deconvolution before or after ComBat in methylation EWAS?
methylation limma ewas combat deconvolution
written by Eliveri • Eliveri
0 votes
2 replies
665 views
Worrisome dispersion plot interpretation (DESeq2)
plot deseq2 deseq2 dispersion
written by IrK IrK
0 votes
0 replies
441 views
News: Workshop: Virtual Variant Detection, January 27–29, 2026
uconn bioinformatics genomics workshop variantdetection
written by zsc25001 • zsc25001
2 votes
1 reply
924 views
Is it expected for T cells to be broadly distributed across the UMAP in pancreatic cancer scRNA-seq data?
annotation single_cell_rna_seq umap scrna-seq
updated by yura.grabovska • yura.grabovska • written by Bum • Bum
1 vote
0 replies
415 views
What percentage of intronic reads should I expect in single-cell vs single-nuclei?
intronic velocyto single-nuclei
written by txema.heredia • txema.heredia
1 vote
0 replies
529 views
News: Introductory NGS data analysis & variant calling workshops (online and in-person, early 2026)
sam mapping fastq variants vcf
written by ecSeq Bioinformatics • ecSeq Bioinformatics
5 votes
9 replies
52K views
Merging/Concatenating Vcf Files
vcftools snp indel
updated by xosiy87829 • xosiy87829 • written by bioinfo bioinfo
1 vote
4 replies
829 views
Tool: Viral Vector Analyzer - Free Web Tool for AAV/Lentivirus/Adenovirus Analysis
gene-therapy viral-vectors
written by Graham • Graham
0 votes
2 replies
801 views
News: Developing Shiny Apps with AI - online course in February
r shiny genomics ai
written by Physalia-courses Physalia-courses
1 vote
1 reply
593 views
Gene Fusion Prioritization Tool
gene vus fusions variants
updated by bioruffo • bioruffo • written by anuthyagatur • anuthyagatur
0 votes
2 replies
11K views
Any alternative way to do ASAP (Assemble Species by Automatic Partitioning)?
delimitation species test
updated by Paul • Paul • written by Chatchapon • Chatchapon
0 votes
0 replies
601 views
News: Unlock Deep Learning Skills with Live Online Training in Python and R
python learning deep
updated by GenoMax GenoMax • written by oliverhooker oliverhooker
0 votes
7 replies
1.4K views
Forum: Is a cross-species scRNA-seq analysis publishable as a hypothesis-generating study without wet-lab validation?
scrnaseq transcriptomics
written by Kurayi Chawatama • Kurayi Chawatama
0 votes
1 reply
458 views
WGCNA basic queries
wgcna
updated by andres.firrincieli andres.firrincieli • written by NEWBIE • NEWBIE
0 votes
2 replies
536 views
Metabat2 Segmentation fault
segmentationfault metabat2
written by Edson • Edson
0 votes
1 reply
511 views
Large GEO dataset download
dnam geo
updated by GenoMax GenoMax • written by jh1997 • jh1997
0 votes
1 reply
617 views
R Package PopGenome Will Not Read in Tab indexed VCF file using ReadVCF
r vcf popgenome
updated by GenoMax GenoMax • written by owjepson • owjepson
1 vote
0 replies
502 views
Job: Computational Biologist position at the University of Alabama at Birmingham
hiring spatial transcriptomics single-cell rna
written by lianov • lianov
1 vote
0 replies
536 views
News: A Complete PYTHON Training Pathway for Biologists, Ecologists and Data-Driven Researchers
python
updated by GenoMax GenoMax • written by oliverhooker oliverhooker
2 votes
2 replies
3.8K views
EggNOG-mapper problem
eggnog
updated by SomeOne • SomeOne • written by Salma • Salma
0 votes
7 replies
1.3K views
Braker3 ETP annotations Filtering
braker3 annotations fungi
written by SomeOne • SomeOne
0 votes
0 replies
392 views
News: GWAS course - online 18-22 May
imputation gwas r plink
written by Physalia-courses Physalia-courses
0 votes
2 replies
951 views
Looking for bulk RNA-seq deconvolution papers
rna-seq bulk rnaseq deconvolution python
updated by coolmattmonkey • coolmattmonkey • written by AlexStar • AlexStar
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