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0 replies
672 views
News: EMBL-EBI Training course | Data science for life scientists: hands-on machine learning for biological data
bioinformatics data science
written by EMBL Events EMBL Events
0 votes
3 replies
694 views
adding features to gtf file using agat tool function (2026)
agat gtf
written by jon50250 • jon50250
0 votes
1 reply
489 views
vg index -j hangs for weeks (minigraph-cactus pangenome, dist file incomplete or missing distance information)
vg
updated by Xian • Xian • written by RianeZ • RianeZ
0 votes
1 reply
381 views
controls for chip-seq real time PCR of h4k16Ac (293 cells)
chip-seq
updated by ATpoint ATpoint • written by adielb • adielb
0 votes
3 replies
817 views
Should I be concerned about warning in DESeq2?
deseq2
updated by swbarnes2 swbarnes2 • written by bioinfo • bioinfo
0 votes
2 replies
1.4K views
Differential Gene Expression Analysis with DESeq2: Is it correct to just get a subset of genes that is of interest and carry out DEG Analysis just for those genes?
rna-seq deseq2 differential_gene_expression
written by Savindu • Savindu
1 vote
31 replies
4.7K views
Tool: [Tool] scVAE-Annotator: Automated Cell Type Annotation for scRNA-seq with VAE and Adaptive Marker Discovery
scrna-seq python machine-learning cell-type-annotation tool
written by or4k2l • or4k2l
1 vote
8 replies
3.7K views
adding features to gtf file using agat tool function
gtf
updated by GenoMax GenoMax • written by 1769mkc 1769mkc
0 votes
2 replies
521 views
Cellchat rankNet information flow
cellchat single cell
written by Nir • Nir
0 votes
3 replies
739 views
Shape of PC loadings
pca rnaseq
written by 131bco • 131bco
0 votes
1 reply
440 views
Multiplex Primer Design to Distinguish 7 Bacterial Genomes
primer qpcr microbiome
updated by colindaven colindaven • written by dirtees • dirtees
0 votes
0 replies
282 views
Forum: What’s the minimum evidence you’d need to trust an AI triage tool saves time without missing P/LP calls?
software ngs bioinformatician pipeline
written by sameer • sameer
0 votes
0 replies
319 views
Query regarding GTEx sample ID structure, histology matching, and new tissue sites in GTEx v11
rna-seq gtex
written by mukherjeeliana24 • mukherjeeliana24
0 votes
0 replies
500 views
Best practice for filtering VEP-annotated variants for Protein Language Model training (EVE/AlphaMissense/ProtBert/ESM): Canonical vs. All Isoforms?
variantfiltering genomics vep gnomad
written by Mustafa • Mustafa
0 votes
1 reply
497 views
Problem loading 10X RNA-seq data in R: length of Dimnames[[1]] mismatch
read10x seurat rnaseq r
updated by Wayne Wayne • written by samuelwatson0711 • samuelwatson0711
0 votes
3 replies
748 views
makeTxDbFromBiomart cannot open URL
database txdb biomart ensembl
updated by GenoMax GenoMax • written by npont • npont
0 votes
1 reply
1.5K views
News: Introduction to Deep Learning for Biologists - online course, 3–7 November
machinelearning python deeplearning convolutionalneuralnetwork
written by Physalia-courses Physalia-courses
0 votes
0 replies
255 views
Need help with jagged plotProfile results in deepTools (LACE-seq data)
deeptools
written by zhuzhuzhu • zhuzhuzhu
0 votes
2 replies
546 views
Extracting sequences from tBlastn results(BLAST standalone)
tblastn
written by bpaddy • bpaddy
0 votes
0 replies
369 views
Tool: PROTAC-Linker-Pathfinder: Automated exit vector identification for undruggable targets (Python/Monte Carlo)
protac python drug-design structural-biology tool
written by BioMed_Student • BioMed_Student
0 votes
7 replies
1.2K views
Tool: VATAligner for Beta testing
software alignment homology tgs ngs
updated by ATpoint ATpoint • written by xuan13hao • xuan13hao
0 votes
1 reply
381 views
Improve predictive models and validation based on ROC
validation predictive-models roc rnaseq
updated by Ram Ram • written by n@4j • n@4j
0 votes
2 replies
596 views
Trimmomatic
rna-seq quality-control fastq trimmomatic galaxy
updated by Istvan Albert Istvan Albert • written by Mahsa80.mm • Mahsa80.mm
0 votes
0 replies
208 views
Partititon whole mitogenomes from different genera for BEAST analysis
partitionfinder partition beast annotation mitogenomes
written by Álvaro • Álvaro
0 votes
0 replies
301 views
News: Spring School in Bioinformatics - ONLINE, 8-12 JUNE
genomeassembly docker rnaseq phylogenomics ngs
written by Physalia-courses Physalia-courses
0 votes
1 reply
506 views
MuSE calls too few snps
muse snp
updated by Ram Ram • written by yalin • yalin
0 votes
6 replies
1.1K views
Best pipeline to merge multiple consumer raw DNA files into PLINK + EIGENSTRAT (1240K) with minimal SNP loss?
dna
written by Persona • Persona
0 votes
5 replies
1.0K views
RNA-seq (bacteria): two control replicates look TR-like (PCA + marker genes).
exclude pca rnaseq limma outlier
updated by Dunois • Dunois • written by MaxMin • MaxMin
0 votes
3 replies
732 views
dbGAP control data access download
dbgap
updated by Ram Ram • written by shrivastav.k.abhi • shrivastav.k.abhi
0 votes
25 replies
3.7K views
-taxids option in blast is not working
blast
written by caroline.zanchi • caroline.zanchi
0 votes
0 replies
221 views
MaGECK MLE Error: TypeError: unsupported operand type(s) for +: 'float' and 'list'
mageck python cripsr
updated by GenoMax GenoMax • written by jamie3355 • jamie3355
0 votes
0 replies
248 views
selscan XPEHH map file
selscan mapfile
updated by Ram Ram • written by SeoG • SeoG
0 votes
3 replies
637 views
May I use Seurat package to analyze single cell RT-qPCR datasets generated on Biomark FluidigmN
seurat biomark pcr fluidigm single-cell
updated by ATpoint ATpoint • written by francois-xavier.mauvais • francois-xavier.mauvais
1 vote
1 reply
539 views
News: Last call: In-person NGS data analysis & variant calling workshop (Munich, Feb 24–26, 2026)
mapping dnaseq rnaseq illumina variantcalling
written by ecSeq Bioinformatics • ecSeq Bioinformatics
0 votes
1 reply
358 views
Positive QMEAN Z-scoring
qmean.
updated by Mensur Dlakic Mensur Dlakic • written by Wided • Wided
0 votes
0 replies
358 views
Job: Postdoctoral Fellow in Computational Human Epigenetics | Oslo, Norway
epigenetics bioinformatics epidemiology
written by Arup Ghosh Arup Ghosh
0 votes
8 replies
1.4K views
find and count motif in FASTA file
grep python seqtk
updated by lieven.sterck lieven.sterck • written by Matteo Ungaro • Matteo Ungaro
6 votes
14 replies
9.5K views
Forum: The ethics of sci-hub
paywall open-science journals publications
updated by Garry F. Goods • Garry F. Goods • written by WouterDeCoster WouterDeCoster
0 votes
2 replies
507 views
Transfer gene ontology annotations
go-term annotation gene-ontology
updated by Ram Ram • written by ja569116 • ja569116
1 vote
0 replies
570 views
Job: PhD (Doctoral) position - Data Sciences for Molecular Systems Biology & Bioinformatics
bioinformatics biomedicine phd data-science r
written by a_gruber • a_gruber
0 votes
5 replies
882 views
BED File from COSMIC Mutation
cosmic
updated by michael.ante michael.ante • written by anasjamshed anasjamshed
0 votes
4 replies
734 views
hisat2 error
alignment hisat2
updated by Arup Ghosh Arup Ghosh • written by aj123 aj123
0 votes
0 replies
273 views
Setting Thresholds and Interpreting Results in GWAS
gwas rna-seq
written by CaERu _no_ korokoro • CaERu _no_ korokoro
0 votes
4 replies
946 views
hifiasm de novo assembly produces short contigs that translate to chromosomes longer than reference
hifiasm ngs denovo
updated by Ram Ram • written by ferran.llobet • ferran.llobet
0 votes
2 replies
613 views
Interpretation of DEG Analysis Results
deseq2 deg
updated by GenoMax GenoMax • written by CaERu _no_ korokoro • CaERu _no_ korokoro
0 votes
2 replies
786 views
PCA, Pearson correlation
pca chipseq
updated by Ram Ram • written by Irving • Irving
0 votes
3 replies
997 views
~80% duplicate pairs in RNAseq data
rna-seq
updated by GenoMax GenoMax • written by Irvin • Irvin
0 votes
4 replies
1.2K views
How to choose BLAST identity and coverage thresholds without excluding true homologs?
metagenomics
updated by Mensur Dlakic Mensur Dlakic • written by Sachin • Sachin
0 votes
5 replies
1.1K views
Gene coverage in Genome Analysis
coverage ensembl genome
written by npont • npont
0 votes
0 replies
369 views
News: Machine Learning with R & tidymodels (16–20 Feb)
machinelearning omics genomics ai r
written by Physalia-courses Physalia-courses
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