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0 votes
1 reply
618 views
Raw reads or raw counts for meta-analysis
meta-analysis rna-seq htseq star
updated by Ram 4.5K • | written by a.stef.44 1
0 votes
7 replies
2.2K views
Can someone explain to me in relatively simple language how PCR primer, reverse primer, and probe sequences are generated?
reverse probe pcr primer
written by Ryan 0
2 votes
12 replies
5.5K views
Tool: fastplong: ultrafast preprocessing and quality control for long reads
long-reads
updated by lieven.sterck 1.6K • | written by chen 253
1 vote
8 replies
2.8K views
GEOquery issue
database geo geoquery
written by Jaber 3
0 votes
0 replies
874 views
Need help with providing RNA Seq data to run braker.pl in ETP mode.
augustus gene-prediction rna-seq braker genemark
written by Vijith 10
0 votes
2 replies
820 views
Can the CFD commercial code simulate multicellular flow in a tall cavity using the second-order scheme?
multicellular-flow
updated by GenoMax 16K • | written by uthai 0
0 votes
1 reply
715 views
How to filter a reference genome for single copy genes?
busco genes single copy genome
updated by Pierre Lindenbaum 17K • | written by Armin 1
1 vote
0 replies
1.3K views
Job: Full Time Bioinformatics Analyst: University of Connecticut: Computational Biology Core
bioinformatician
written by Jlw 20
0 votes
0 replies
393 views
How to obtain level 2 GO term from go_id?
r go-term
written by Chenfeng 0
3 votes
0 replies
746 views
Blog: How Seurat PCA projection, KNN and MNN for label transfer work
r rna-seq scrna-seq pca
updated by Ram 4.5K • | written by Ming Tommy Tang 469
0 votes
0 replies
631 views
News: RNAseq in non-model organisms (online course)
bioconductor pacbio rnaseq
written by Physalia-courses 281
0 votes
3 replies
972 views
WGS sequence composition bias at read start
wgs
updated by GenoMax 16K • | written by Mega 0
0 votes
2 replies
1.2K views
Does BWA-MEM 2 account for overlapping reads within the paired end data?
mapping calling variant reads
written by ayeraselvan 1
0 votes
0 replies
777 views
Forward strand alignment
qc gwas strand alignment
written by JJ 0
0 votes
2 replies
1.2K views
Is there an easy way to scrape a whole bunch of NCBI RefSeq FASTA from NCBI?
fasta genome ncbi
updated by shenwei356 870 • | written by Mark 6
0 votes
0 replies
1.0K views
Trouble with CGenFF and Ligand Topology in GROMACS
cgneff protein-ligand gromacs md simulation
written by Mostafa 0
0 votes
3 replies
2.1K views
GTF merging with gffcompare
gtf merging annotation rna-long reads transcript
updated by Carlo Yague 906 • | written by Mai.Nabil 0
0 votes
2 replies
1.1K views
How methylation is annotated, and what is the number behind the cg.... annotation
methylation annotation
written by Peter 0
0 votes
1 reply
798 views
Rnbeads
rnbeads methylation dna r
updated by Ram 4.5K • | written by AJ 0
0 votes
5 replies
1.9K views
Overlapping reads, how do we counter them while variant calling, haplotype calling, does it affect mapping?
reads
updated by Jeremy Leipzig 2.3K • | written by ayeraselvan 1
0 votes
1 reply
987 views
(Nextflow) Having trouble with a generic process to stitch together files that were split and then processed
nextflow
updated by Pierre Lindenbaum 17K • | written by sodiumnitrate 2
0 votes
5 replies
1.9K views
Gene set enrichment analysis gives a lot of "significant pathways"
kegg gesa hallmarks
written by Nona 9
0 votes
2 replies
975 views
How can I check if a variant affects a TFBS?
variant tfbs
written by Tania 18
0 votes
0 replies
434 views
How to estimate haplotype number from pooled dna sequences from blood samples
sequences haplotype pooled
written by Arline 0
0 votes
0 replies
420 views
Deduplication when performing copy number analysis
cnv copy-number
updated by Ram 4.5K • | written by Kenji YT 0
0 votes
2 replies
953 views
intersection of vcf files with bcf isec - no header
isec bcftools vcf
written by jasminestaples1 0
0 votes
2 replies
1.1K views
Cell cluster that mainly consists of doublet.
doublet scrna-seq
updated by jared.andrews07 1.9K • | written by D 0
0 votes
0 replies
781 views
Struggling with Meme-suite, looking for troubleshooting tips
atac-seq meme motifs
written by Ronin 1
0 votes
1 reply
727 views
phyloseq Analysis using R
phyloseq
updated by dthorbur 350 • | written by makujabi 0
0 votes
1 reply
1.4K views
Subsetting post integration in seurat V5
integration seuratv5
updated by kaelin 0 • | written by nina.goldman.21 0
0 votes
5 replies
1.4K views
weird entry in EnhanceVolcano Volcano plot -RNAseq
deseq2 rnaseq enhancedvolcano
updated by Ram 4.5K • | written by Yogesh 0
0 votes
5 replies
2.9K views
SNPs of a specific mouse strain
snp
updated by ayayron117 1 • | written by darklings 60
0 votes
6 replies
2.5K views
Problem with mapping rate while using Mageck to process CRISPR screen data
crispr screen crispr-screen mageck mapping
updated by ATpoint 9.1K • | written by Luwell 0
0 votes
2 replies
1.3K views
Basic question but how can I search the NCBI Nucleotide database using BLAST CLI
cli blast
written by Mark 6
0 votes
9 replies
3.2K views
Permutations FGSEA
fgsea
written by Nona 9
0 votes
0 replies
488 views
Bracken+Krakenuniq
bracken krakenuniq bracken-build
written by Bhawna 0
0 votes
5 replies
1.3K views
NextSeq Data Shifted by One Line During Demultiplexing — What Could Cause This?
demultiplexing
updated by Arup Ghosh 402 • | written by pkk 0
0 votes
0 replies
467 views
Too many antimicrobial resistance gene from RGI bwt
wastewater rgi amr arg kraken2
written by Pratibha kadam 0
0 votes
1 reply
783 views
I'm running a scATAC-seq analysis. However, the peaks I've called are somehow weird
macs2 scatac-seq signac
written by Pallondyle 0
0 votes
1 reply
980 views
PacBio long reads and small plasmids
pacbio plasmids illumina long reads
updated by GenoMax 16K • | written by Texx 4
0 votes
1 reply
1.2K views
i am having trouble with the VIBRANT embedded in Viroprofiler
vibrant viroprofiler
updated by GenoMax 16K • | written by krmoon518 3
0 votes
0 replies
1.1K views
HOMER findPeaks: what does -fdr actually do?
homer atac-seq calling peak dap-seq chip-seq
written by mcsimenc 3
0 votes
0 replies
610 views
ChIP-seq Error with rtracklayer
chip-seq rtracklayer
updated by Ram 4.5K • | written by shamzar 0
0 votes
0 replies
560 views
EnrichKEGG issue
kegg overrepresentation enrichment
written by am29 6
0 votes
2 replies
1.1K views
Contig dereplication script not retrieving correct number of sequences
phage ani metagenomics dereplication
written by bksakdinan.12 0
0 votes
2 replies
1.1K views
Multi OMICS analysis
multi-variate multi-omics
updated by Ming Tommy Tang 469 • | written by SKY 6
0 votes
0 replies
582 views
GWAS() function error rrBLUP package for GWAS
r rstudio rrblup gwas
updated by Ram 4.5K • | written by giulia.trauzzi 3
0 votes
4 replies
1.0K views
Mapping PCR primers to human genome
mapping primer aligner
written by Yvan 0
0 votes
0 replies
957 views
Using RunAzimuth with pbmcref on mouse data
mouse scrna-seq suerat azimuth
written by Assa Yeroslaviz 197
0 votes
1 reply
730 views
Mitochondrial Transcriptome Analysis with PacBio RNA Data using Geneious Prime
ngs rna-seq pacbio
updated by Ram 4.5K • | written by hashim.rana11 4
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