This is a test version of Biostars. For the public version, visit https://www.biostars.org.
2 votes
4 replies
3.3K views
R CytoTRACE2 Results: Potency Score of Naive B Cells Lower Than Memory B and Plasmablasts – Contradicts Biological Knowledge
cytotrace2
written by Binghong • Binghong
2 votes
1 reply
1.1K views
Rules for Assigning Start and End Phase to Coding Exons
stare_phase reading_frame end_phase
updated by Jorge - Ensembl • Jorge - Ensembl • written by Milica • Milica
0 votes
1 reply
780 views
converting transcript positions to genomic locations
genomic locations
updated by Pierre Lindenbaum Pierre Lindenbaum • written by baibhu1234 • baibhu1234
0 votes
1 reply
815 views
Read groups RNA-seq and hisat2
hisat2 rna-seq read-groups
updated by Ram Ram • written by frarodmar17 • frarodmar17
0 votes
0 replies
599 views
simulate a genome with specific event
simulation genome retrocopy
written by Matteo Ungaro • Matteo Ungaro
0 votes
4 replies
1.6K views
Help with R programming assignment
r offtopic
updated by Michael Dondrup Michael Dondrup • written by sidhanthbaru • sidhanthbaru
0 votes
1 reply
1.1K views
MSA with extended alphabet
multiple sequence msa alignment
updated by Mensur Dlakic Mensur Dlakic • written by retzl.bernhard • retzl.bernhard
0 votes
0 replies
547 views
Help with Finding SNPs in H. pylori Assembled Genomes
question technical
written by vrao • vrao
0 votes
2 replies
1.1K views
Reference Genome with decoy for RNAseq data processing
star salmon rnaseq
updated by Zhenyu Zhang Zhenyu Zhang • written by cwwong13 • cwwong13
0 votes
1 reply
750 views
multiple ATG SEQUENCE
multipe sequence atg offtopic
updated by Carlo Yague Carlo Yague • written by Sijjil • Sijjil
0 votes
0 replies
867 views
Keep SNP's with call rate greater than 30 in VCF
snp call rate roh genotyping
updated by Bastien Hervé Bastien Hervé • written by Smilesky • Smilesky
0 votes
10 replies
7.3K views
Buy PC for metagenomics
assembly alignment genome next-gen
updated by Joe Joe • written by lugonauta • lugonauta
0 votes
0 replies
700 views
Dynamic tree cut results
clustering deg
written by Mai.Nabil • Mai.Nabil
0 votes
0 replies
577 views
Significant variation in FastQ File Sizes - WES
libraryprep wes human
written by irate.pirate • irate.pirate
0 votes
2 replies
1.4K views
Repetitive sequences analysis
repetitive sequences
updated by rfran010 • rfran010 • written by frarodmar17 • frarodmar17
0 votes
1 reply
849 views
Protein secuence extraction from gpff file for a genes list
protein gpff
updated by JC JC • written by reza reza
1 vote
0 replies
1.4K views
Job: Postdoctoral Researcher in Single-Cell Sequencing Analysis
researcher post-doctoral
written by ejvandenoord • ejvandenoord
0 votes
2 replies
1.2K views
filtering heterozygous genotypes
bcftools heterozygous
written by Bushra Bibi Bushra Bibi
2 votes
0 replies
871 views
An alternative to haplotype phasing - assembly?
samtools assembly bam spades phasing
written by Jess • Jess
0 votes
0 replies
587 views
how to make/get the SNP annotation file for running TORUS
torus annotation snp gwas
updated by GenoMax GenoMax • written by lee • lee
0 votes
1 reply
756 views
Flongle 16srRNA nanopore sequencing
r.9.4.1 flow flongle
updated by GenoMax GenoMax • written by Major ginger • Major ginger
0 votes
2 replies
1.2K views
Genomes with existing T2T assemblies?
t2t genome assembly reference
updated by cmdcolin cmdcolin • written by predeus predeus
0 votes
2 replies
1.9K views
Installing ggpubr in Docker container rocker/r-base??
ggpubr dockerfile docker r
updated by Ram Ram • written by giulia.trauzzi • giulia.trauzzi
0 votes
0 replies
669 views
News: Bayesian Data Analysis in R course - online, March 3-7
bayesian statistics r
written by Physalia-courses Physalia-courses
1 vote
5 replies
1.8K views
Should we cite sed / awk in papers?
sed awk
updated by ATpoint ATpoint • written by hello_bioinf • hello_bioinf
0 votes
0 replies
421 views
Briggs model parametes
briggs damage gargammel
written by anna.utili • anna.utili
0 votes
10 replies
2.4K views
Nanopore sequencing - plasmid assembly length confusion
assembly long nanopore reads
written by abedkurdi10 • abedkurdi10
0 votes
0 replies
836 views
Post-GWAS colocalization analysis VS. variant annotation, what's the difference?
variant annotation snp colocalization gwas
written by sarfdezco • sarfdezco
0 votes
2 replies
980 views
EDTA ,Transposon detection, repeat modeler and masker
homology_based_te_detection fragmentedte
written by manaswiniparija3 • manaswiniparija3
0 votes
8 replies
2.2K views
Finding ORFs in a FASTA file
matrixscore orfs motifs motifscoring
updated by Michael Dondrup Michael Dondrup • written by Melissa • Melissa
0 votes
3 replies
1.5K views
Ilumina Low quality bases at first 20 cycles + PHIX problem
sequencing
updated by GenoMax GenoMax • written by alenew.am • alenew.am
0 votes
3 replies
974 views
HMP dataset download issue (403 error)
hmp dataset microbiome
updated by GenoMax GenoMax • written by Emily • Emily
0 votes
1 reply
695 views
Enrichment Analysis for cCREs
enrichment statstest bedtools ccres fishers
updated by ATpoint ATpoint • written by alexanderturco1 • alexanderturco1
1 vote
0 replies
1.3K views
Job: Postdoctoral Research Position: Developing Novel Algorithms and Tools for Circular RNA Analysis
circrnas biology bioinformatics computational
written by Tobias Jakobi • Tobias Jakobi
0 votes
10 replies
2.6K views
Per Sequence GC Content fail
fastqc
written by Eduardo • Eduardo
1 vote
7 replies
8.3K views
has anyone use trimmomatic with NEBNext adaptors
rna-seq
updated by Johan Zicola Johan Zicola • written by eozcan • eozcan
2 votes
1 reply
937 views
t2t RNAseq alignment with STAR
chm13 t2t rnaseq
updated by GenoMax GenoMax • written by eva_u • eva_u
2 votes
0 replies
25K views
Herald: The Biostar Herald for Wednesday, February 19, 2025
herald
written by Biostar Biostar
1 vote
3 replies
1.2K views
Cell Type annotation showing Normal cells as Cancer cells in single cell analysis.
seurat single_cell spatial-transcriptomics
updated by rfran010 • rfran010 • written by geno89 • geno89
0 votes
0 replies
457 views
Running NGSadmix with pre-calculated ancestral genotype freqs?
admixture ngsadmix lcwgs angsd
written by j.smout.1 • j.smout.1
0 votes
5 replies
1.6K views
Differential Expression and absolute expression
normal deg tumour rna
written by jain72744 • jain72744
0 votes
3 replies
1.1K views
Mapping ~100-300bp Sequences to Metagenome-Assembled Genomes
assembly metagenomic
updated by GenoMax GenoMax • written by chemokine-1 • chemokine-1
0 votes
1 reply
625 views
Differetial analysis EdgeR
differetial edger analysis
updated by ATpoint ATpoint • written by maryak • maryak
0 votes
3 replies
828 views
Can I compare Gene Expression in T Cells from PBMCs vs. Tumor Tissue (for match samples)?
batch-effect-correction integration scrnaseq single-cell harmony
updated by ATpoint ATpoint • written by Saeedeh Salehi • Saeedeh Salehi
0 votes
2 replies
931 views
multiBamSummary
deeptools multibamsummary reverse-strand
updated by Ram Ram • written by Hina • Hina
0 votes
2 replies
1.2K views
How to convert CIGAR string to VCF?
cigar vcf alignment variant-calling
updated by Ram Ram • written by Biomonika (Noolean) Biomonika (Noolean)
0 votes
6 replies
1.5K views
Extract FUngal mRNA from RNAseq Data
rnaseq rna fungus humant2t transcriptomics
written by SomeOne • SomeOne
0 votes
1 reply
1.0K views
PyMOL truncates part of structure when exporting as .pdb
pymol
updated by Mensur Dlakic Mensur Dlakic • written by jdoesstuff • jdoesstuff
0 votes
0 replies
1.8K views
Job: Research Technician (Bioinformatics Analyst) - Computational Biology Core, Institute for Systems Genomics, UConn
bioinformatics-analyst research-technician
written by spu24005 • spu24005
0 votes
1 reply
785 views
CLI Downloading from Human Microbiome Project Given Login + contact failure
microbiome hmp
updated by Arup Ghosh Arup Ghosh • written by Maxwell • Maxwell
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