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0 votes
1 reply
1.2K views
DESeq2 on a complex design
rna-seq deseq2
updated by BioinfGuru BioinfGuru • written by Chengrui • Chengrui
0 votes
0 replies
713 views
Visium spatial transcriptomics slide with two tissue samples
spatial-transcriptomics visum
updated by Ram Ram • written by Chirag Nepal Chirag Nepal
0 votes
2 replies
1.5K views
Alternative Visualization Methods for CLANS Output: Using Cytoscape or Other Tools
visualization clans cytoscape network
written by Rohan • Rohan
0 votes
3 replies
1.6K views
Samtools flagstat output said there is zero paired-reads in scRNA-seq bam file
samtools flagstat scrnaseq
updated by swbarnes2 swbarnes2 • written by juyeonlee • juyeonlee
1 vote
3 replies
4.1K views
Downloading 1000genome phase 3 data in 38 build
1000genomes
updated by nareshmvr • nareshmvr • written by AVA • AVA
0 votes
2 replies
1.0K views
Discrepancy in HISAT2 Alignment Rates for Paired-End vs. Single-End Reads
ngs hisat2 rna-seq alignment
written by Ujjawal • Ujjawal
0 votes
0 replies
570 views
Copy number: summarize segmented regions into chromosome arms
chromosome-arm copy-number
updated by Ram Ram • written by Ezhil La • Ezhil La
0 votes
6 replies
2.8K views
How to check if all read names are paired properly in paired end fastq.gz files?
samtools illumina paired-end
updated by LChart LChart • written by DNAngel DNAngel
0 votes
0 replies
757 views
SMART-seq normalizarion and integration
smart-seq single-nuclei normalization rna-seq single-cell
written by vigbr • vigbr
0 votes
0 replies
435 views
results comparison from KARGA, kma and amr-meta
karga amr-meta kma
updated by Ram Ram • written by minoonasr • minoonasr
0 votes
4 replies
1.6K views
PolyA in FastQC results from EM-Seq?
fastqc wgbs polya methylation emseq
written by Watermelon • Watermelon
0 votes
1 reply
1.5K views
How is the observed frequencies obtained via HHBlits?
drnapred protein hhblits hmm
updated by Mensur Dlakic Mensur Dlakic • written by jet • jet
0 votes
0 replies
527 views
filtering blastx output file
blastx query
written by Bushra Bibi Bushra Bibi
0 votes
2 replies
884 views
Where can I find mutation status in GEO?
geo
written by Nona • Nona
0 votes
1 reply
696 views
Empty VCF after Strelka
strelka snp wgs strelka2
updated by Pierre Lindenbaum Pierre Lindenbaum • written by isMM • isMM
3 votes
12 replies
5.5K views
Forum: How to improve a bioinformatics study and paper so that it is not considered "too preliminary" by publishers and journals?
deg journal
updated by Matthias Zepper Matthias Zepper • written by egascon • egascon
0 votes
1 reply
726 views
Using BLASTp with Custom Substitution Matrices
blastp
updated by biofalconch biofalconch • written by H.Hasani H.Hasani
0 votes
0 replies
568 views
Person neoplasm cancer status
tumor neoplasm
updated by Ram Ram • written by maryak • maryak
1 vote
6 replies
1.7K views
Use LiftOver without knowing reference Fasta of used vcf file
vcf
updated by GenoMax GenoMax • written by Lukas • Lukas
0 votes
0 replies
684 views
How to convert Illumina SNP ids having mappings to multiple RSID in loci to rsid file ?
23me illumina conversion gsa3.0
written by vasank1958 • vasank1958
0 votes
1 reply
931 views
Proteomes: measured or predicted from genomes/transcriptomes?
genome proteome
updated by Mensur Dlakic Mensur Dlakic • written by dec986 dec986
0 votes
11 replies
4.5K views
Rosetta make_fragments.pl fails: "Cannot initialize readdb for nr database"
software-error nr rosetta
updated by Noora • Noora • written by Alex_I • Alex_I
0 votes
1 reply
1.0K views
How to choose cells that co-express several genes with scanpy?
scanpy scrna-seq single-cell
updated by LChart LChart • written by bioinfo • bioinfo
0 votes
8 replies
2.3K views
featureCounts output
macs2 featurecounts
updated by rfran010 • rfran010 • written by QX • QX
0 votes
4 replies
1.2K views
Replace SB flag by PASS in my VCF file
vcf
written by ManuelDB ManuelDB
0 votes
0 replies
922 views
Job: PhD Position "A Bioinformatics Approach to Analyzing Epistatic Interactions in Enzymes using Long-read Sequencing Data"
machine-learning deep-mutational-scanning oxford-nanopore
updated by Ram Ram • written by funcgen • funcgen
0 votes
0 replies
1.2K views
Job: Request for Proposals: Amazon Web Services (AWS) Web Development & Bioinformatics Consultant
aws ffar
updated by Ram Ram • written by ffarhr • ffarhr
0 votes
6 replies
1.7K views
Selecting top signature score from signatures with different distributions
r classification distributions
updated by LChart LChart • written by yura.grabovska • yura.grabovska
0 votes
1 reply
924 views
How to download the mutational signatures for TCGA?
mutational-signature tcga
updated by LChart LChart • written by Camelia • Camelia
0 votes
11 replies
2.9K views
sequenceTubeMap input files
sequencetubemap vg
updated by Jordan M Eizenga • Jordan M Eizenga • written by Mayank • Mayank
0 votes
5 replies
1.6K views
Trimmomatic in windows
windows 16s trimmomatic
updated by GenoMax GenoMax • written by Clíona • Clíona
0 votes
4 replies
1.2K views
high no_feature in htseq
rna-seq htseq-count quantification
written by Srujana • Srujana
2 votes
6 replies
5.0K views
meaning of "primary alignment" in samtools
samtools
updated by i.sudbery i.sudbery • written by Francois Piumi • Francois Piumi
0 votes
2 replies
778 views
bacterial microRNA data retrieval
mirna bacteria srna
written by praddyumnapdm • praddyumnapdm
0 votes
1 reply
1.2K views
How do I Download Metagenomics Data from NCBI or EBI with its Metadata?
metagenomics ncbi
updated by Ram Ram • written by John • John
0 votes
5 replies
1.3K views
Transcription factors associated with Differentially Expressed Genes
transcription enrichment
written by kamalika • kamalika
0 votes
1 reply
784 views
GATK4 VariantAnnotator deletes variants
gatk4
updated by Pierre Lindenbaum Pierre Lindenbaum • written by ManuelDB ManuelDB
0 votes
4 replies
1.2K views
filtering blastx output file
blastx query filtration coverage
written by Bushra Bibi Bushra Bibi
0 votes
0 replies
579 views
TCGA methylation analysis
methylation
written by ali • ali
0 votes
0 replies
592 views
Heterozygous SNPs Allele Balance Threshold
snps genomics wes allele
updated by Ram Ram • written by menyawino • menyawino
0 votes
4 replies
2.1K views
Seeking Advice on Multisample Variant Calling Pipeline with BWA-MEME and GATK DRAGEN Mode
gatk variant-calling dragen bwa
written by George • George
0 votes
1 reply
790 views
Filter vcf file by position AND alternative allele
bcftools vcf filter
updated by zx8754 zx8754 • written by _quantum_girl_ • _quantum_girl_
0 votes
3 replies
8.2K views
DESeq2 vst function error
deseq2 rna-seq r normalization
updated by DareDevil DareDevil • written by lenC_biotecLover • lenC_biotecLover
0 votes
5 replies
5.3K views
Get position in GRCh38 from rsid
snp rsid location
updated by Philipp Bayer Philipp Bayer • written by _quantum_girl_ • _quantum_girl_
0 votes
5 replies
1.6K views
Same sample and reference genome, different number of reads
starsolo single-cell rnaseq
updated by rfran010 • rfran010 • written by Nicholas • Nicholas
0 votes
0 replies
430 views
Error Code 104 in STAR Alignment
star
written by deleonalyssa289 • deleonalyssa289
0 votes
1 reply
773 views
How to Subset a Custom BLAST Database for Specific Genes of Interest?
blastp blast
written by samuel.himes • samuel.himes
0 votes
4 replies
1.6K views
Determining library prep: rRNA depletion or mRNA enrichment
rrna library mrna
updated by rfran010 • rfran010 • written by Synanth • Synanth
5 votes
5 replies
27K views
Herald: The Biostar Herald for Monday, July 22, 2024
herald
updated by LChart LChart • written by Biostar Biostar
0 votes
2 replies
989 views
Remove bacterial and protoza from Kraken2 EuPathDB
database kraken2 eupathdb
written by Christopher • Christopher
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