Why are polyA adaptors found in ATAC-seq reads?

I used Trim Galore to remove Nextera adaptors and then conducted QC again. However, polyA was still detected. Is this a normal situation? Should I remake the library?

atacseq

• 666 views • link

updated 2.0 years ago by ATpoint

• written 2.0 years ago by MIKA •

How about first showing some plots before even thinking about redoing an expensive experiment?

• 0 views • link

written 2.0 years ago by ATpoint

0 answers

No answers yet.

More posts like this

FastQC polyA and polyG trimming in RNA-seq
written 1.6 years ago by joonhong kwon •

Hi! I have raw Fastq data with 151bp paired-end reads, which were trimmed using Trim Galore (v0.6.10). After trimming, I observed overrepresented polyG sequences (purple …
Discrepancies in Sequence Length Distribution Between Trim Galore and Trimmomatic During Adapter Tr…
written 2.1 years ago by George •

Hello everyone, I am currently facing an unusual situation while performing adapter trimming using both Trim Galore and Trimmomatic. I would appreciate any insights or …
After Trim galore!, fastqc show the seuqence length is 20-151
written 3.8 years ago by Jiahui •

Hi all, I run fastqc and multiqc and found that my RNA-seq has adaptors exists, after using trim galore! I run fastqc to see if …
Trimming SOLiD adapters
written 5.1 years ago by kimmitzka •

Hi, Which tool should I use to trim adaptors in SOLiD files? It seems that trim-galore can not be used in SOLiD. Thanks a lot! …
Issues with adapter trimming when there are no adapters?
written 5.7 years ago by DareDevil

When I run fastqc on my sample, it did not identify any adaptor sequence in the sample but there were some low quality reads in …
Cutadapt terminated with exit signal: '24'.
written 6.4 years ago by roy.granit

I'm using Trim Galore to process RNAseq and in one of the samples in get this error: > Cutadapt terminated with exit signal: '24'. Terminating …
Effect of trimming on alignment and fastqc report.
written 7.4 years ago by kspata

Hi All, I have MiSeq PE250 reads for a viral vector sample. I trimmed the raw reads with Trim Galore (length >= 200 and Q …
Insert size smaller than the read lenght
written 8.0 years ago by mariaroviscomonteiro •

Good morning, I know this topic has been discussed but I still have some doubts in its regard. I've done RNA seq 2x150 bp and …
Issues with Illumina Universal Adapters
written 8.2 years ago by williamsbrian5064

Hi, I am attempting to trim some RNA-Seq data. I am having some issues with Illumina universal adapters. I am using trimmomatic to trim the …
trim_galore finds a different adaptor than the one I expected...
written 9.8 years ago by blur

Hi, I used trim galore to automatically cut adaptor sequences, and it cut the adaptor AGATCGGAAGAGC. this is the defult used by trim galore, according …