This is a test version of Biostars. For the public version, visit https://www.biostars.org.
Error in GATK BaseRecalibrator

I am trying to run gatk base recaliberator but keep getting this error Error showing java.lang.RuntimeException: Invalid deflate block found.

I have tried samtools quickcheck on my bam but there was no output (assuming it means no error) The BAM is 11gb. I have run many samples before using the same command but this one is really confusing me. The command I use-

singularity exec docker://broadinstitute/gatk:4.4.0.0 gatk BaseRecalibrator --intervals $intervals --interval-padding 100 -I ${ID}_P_markdup.bam -R $hg38 --known-sites $mills --known-sites $phase --known-sites $dbsnp -O ${ID}_P_recal_data.table --tmp-dir $tmp 

Please help figuring out the issue.

baserecalibrator gatk

invalid deflate block found

Looks like you may have a corrupt input file.

Please do not paste screenshots of plain text content, it is counterproductive. You can copy paste the content directly here (using the code formatting option shown below), or use a GitHub Gist if the content volume exceeds allowed length here.

code_formatting

0 answers

No answers yet.

Log in to answer this question.