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0 votes
1 reply
175 views
Should QC cutoffs be done per-sample or one global cutoff for snRNA-seq with very different samples?
quality snrnaseq seurat control
updated by ATpoint 9.1K • | written by dxj294 0
0 votes
0 replies
180 views
News: Single-cell analysis course & certification
singlecell bioinformatics rnaseq ngs
updated by GenoMax 16K • | written by Nicole 12
0 votes
4 replies
305 views
Gold standard / Phylogenetics for intertidal fish
phylogenetics intertidal busco orthologs
updated by samuel.a.odonnell 86 • | written by thrivenow 0
7 votes
2 replies
405 views
Tool: FindSSU — fast 16S/18S classification, comparison, and sequence retrieval for 276,000 organisms via website, API, or command-line tool
ssu 16s bbtools taxonomy
updated by Dunois 301 • | written by Brian Bushnell 2.0K
0 votes
0 replies
101 views
Tool: ALIEN: pure-Python GMT builder with MSigDB/Enrichr downloads for namespace-specific gene-set libraries
python gsea msigdb enrichr gmt
written by Denis Demin 3
0 votes
3 replies
637 views
Dealing with massive CNV hyper-calling in DRAGEN Germline Enrichment: Best post-hoc VCF filtering and cohort collapse strategy?
cnv truvari whole-exome bcftools-filtering dragen
updated by LauferVA 497 • | written by yawolberg 1
0 votes
0 replies
129 views
News: Agentic AI for Life Sciences – Online course (6–7 July)
genomics ai agenticai
written by Physalia-courses 281
0 votes
1 reply
279 views
Tool: Exploring forensic STR matching from consumer WGS data (experimental pipeline)
genomics bioinformatics forensics
updated by jared.andrews07 1.9K • | written by Spike Tree 0
0 votes
0 replies
115 views
Tool: edge2torch — flexible PyTorch models for knowledge-primed neural networks
software python machine-learning neural-networks systems-biology
written by Thomas 4
0 votes
1 reply
822 views
q-value threshold for omics data when performing univariate cox analysis
machinelearning q-value cox fdr omics
updated by i.sudbery 2.3K • | written by Yugan Gogul Muthukumar 3
0 votes
0 replies
425 views
Job: Hiring: Full-Time & Part-Time Bioinformatics Researchers – University of Malta
bioinformatics academia multi-omics
written by jp 13
0 votes
1 reply
225 views
ROC Analysis for a Single Continuous Biomarker
roc auc biomarker learning machine
updated by i.sudbery 2.3K • | written by fnepo 4
0 votes
3 replies
585 views
Addmodulescore permutation
integration seurat sctransform st
updated by jared.andrews07 1.9K • | written by María José 1
0 votes
0 replies
123 views
Tool: GeneFix AI – Open Source Bioinformatics AI Engine for Sequence Analysis, Research Assistance & AI Integration
bioinformatics
written by Mubashir 0
0 votes
0 replies
130 views
News: Introduction to Snakemake (SNKM03)
snakemake
updated by GenoMax 16K • | written by oliverhooker 14
0 votes
0 replies
96 views
News: Final and Last Call - Only 3 Last Places Remaining for the 10th Berlin Summer School in NGS Data Analysis 2026
variantcalling transcriptomics summerschool dnaseq rnaseq
written by ecSeq Bioinformatics 9
0 votes
0 replies
116 views
Job: Director/Senior Director of Research Software Engineering
engineering director research software
updated by colindaven 844 • | written by vanessa 1
0 votes
2 replies
367 views
Doublet Detection in SMART-Seq2 single-cell data
doublets scrnaseq singlecell smartseq2
written by luca8650 0
0 votes
0 replies
92 views
Variant counts decrease across cumulative coverage tiers after genotype masking
vqsr vcf bcftools wgs filtering
written by itsmesmb 0
0 votes
0 replies
123 views
Can vg/minigraph-cactus export a GATK-style all-sites gVCF from a pangenome graph?
gvcf vg
written by RianeZ 0
0 votes
0 replies
86 views
Tool: VarGate > dataviz + scoring of QC metrics (Picard / biobambam2)
vargate
written by Pierre 2
0 votes
0 replies
91 views
Opinions? Using Variable Inclusion Probability (VIP) as a bootstrapping approach to Elastic Net modeling
machine-learning omics elastic-net 16s bootstrap
written by Ana 0
0 votes
0 replies
104 views
News: Online course - Nextflow for beginners
workflowmanager nextflow
written by Physalia-courses 281
5 votes
4 replies
5.2K views
Multiplexed Rnaseq
rna illumina gene
updated by Saurav 0 • | written by Yannick Wurm 252
0 votes
0 replies
106 views
OTU filtering thresholds for soil ITS metabarcoding – none of my tested filters seem appropriate
metabarcoding soil edna its
written by Loïs 0
0 votes
5 replies
1.4K views
How to handle TrEMBL proteins without gene annotation in plasma proteomics?
biomarker uniprot proteomics annotation trembl
updated by domanic 0 • | written by Luwell 0
1 vote
1 reply
720 views
Advice on choosing the best ChromHMM mode for CUT Tag data
cut_tag chromhmm histone
updated by dsdsdsdsdsd 0 • | written by luminea 2
0 votes
1 reply
187 views
What repetitive bioinformatics task do you wish was fully automated?
automation discussion workflow pipeline
updated by Stefanie 1 • | written by Greeshma Reddy 0
0 votes
0 replies
602 views
About peptide design
software error
updated by Jimmy 0 • | written by fatema19.bge 0
0 votes
2 replies
1.3K views
multiple peptide docking
docking peptide
updated by Jimmy 0 • | written by husainbioinfos 0
0 votes
1 reply
615 views
Peptide Toxicity
protein toxinpred3.0 toxicity peptide
updated by Jimmy 0 • | written by Saurabh 0
2 votes
1 reply
190 views
Which locus should be considered the true ortholog after liftOver disagreement between chrUn and chr16 in canFam4 miRNA mapping?
genome-assembly mirna ucsc blat liftover
written by jhonatan.henrique 4
0 votes
1 reply
214 views
Tool: qPCR Analyst: browser-based qPCR analysis tool, no install required
gene-expression qpcr statistics visualization tool
updated by Ram 4.5K • | written by qPCR Analyst 0
0 votes
1 reply
195 views
Forum: should i come to bioinformatics (need help)
new help
updated by Ram 4.5K • | written by mehulgautam600 0
0 votes
0 replies
111 views
News: Spring School in Bioinformatics – Online Course, 8–12 June
singularity genomeassembly phylogenomics rnaseq
written by Physalia-courses 281
0 votes
2 replies
3.1K views
Best way to systematically select ENCODE data to download?
encode
updated by Raquel Moya 0 • | written by Eric Lim 217
0 votes
0 replies
96 views
What is the best approach/workflow to map rMATS alternative splicing events to full-length isoforms?
rmats alternative splicing isoform rna-seq
written by mahsa80.mm 0
0 votes
1 reply
177 views
Question about ONT basecalling
ont basecalling
updated by lieven.sterck 1.6K • | written by Max 0
0 votes
0 replies
112 views
Forum: Career Advice: Independent Institutional Project (Public Data) vs Entry-Level Industry Position
career industry job advice academia
updated by GenoMax 16K • | written by eyaggelia146 3
1 vote
2 replies
426 views
Tool: vcfv - fast VCF parser to Polars/Pandas DataFrame with native INFO and FORMAT expansion
polars python parser vcf
written by rvk20 1
4 votes
0 replies
423 views
Tutorial: Free beginner-friendly tutorial series for single-cell RNA-seq analysis (ngs101.com)
scrna-seq
written by ngs101.com 39
0 votes
0 replies
84 views
Identification of antisense transcripts and/or cryptic transcripts from Stranded total RNA-seq data
antisense transcripts totalrna-seq rnaseq cryptic
written by WannaBeWizard 0
0 votes
0 replies
81 views
News: Seeking Feedback for CPOP V2, A GNN & Ensemble Platform for Catalytic miRNase Design
rna
updated by GenoMax 16K • | written by Joshua 0
0 votes
5 replies
3.7K views
how to distinguish monomer and dimer without wetlab techniques
sequence
updated by Gunjan 0 • | written by dineshtripathy9658 1
0 votes
3 replies
332 views
Distribution of Transcripts per Cell in Spatial Transcriptomics (Xenium)
xenium spatial-transcriptomics spatialdata spatial-omics
updated by Bastien Hervé 680 • | written by npont 3
2 votes
0 replies
141 views
News: 10 Years of Physalia Courses: Celebrating with a 10% Discount for the BioStar Community
training-courses genomics bioinformatics
written by Physalia-courses 281
6 votes
3 replies
8.7K views
Tool: cnv_facets: somatic Copy Number Variant calling using the facets package
cnv somatic facets cancer
updated by yalin 0 • | written by dariober 1.5K
0 votes
5 replies
1.9K views
Forum: Collaboration for phd thesis
next-gen
updated by Medhat 981 • | written by Hope AKKARI 1
0 votes
0 replies
122 views
Assessing mapping quality
quality-check bcf mapping vcf
written by Noctiluca 0
2 votes
0 replies
130 views
Why does quantile transform with uniform output outperform normal output in a deep survival model (C‑index)?
preprocessing omics quantilenormalization normalization deeplearning
written by Yugan Gogul Muthukumar 3
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