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6
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9
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597
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7 follow
Forum: Ideal PC configurations and operating system for bioinformatics laboratory
PC
updated 35 minutes ago by ▴ 120 • written 29 days ago by ▴ 10
0
votes
0
replies
199
views
Forum: Improvement on automatic annotation of single-cell RNA-seq data from the literature using LLM
annotation scRNA llm
4 weeks ago by yxwucq • 0
5
votes
2
replies
388
views
Forum: Bioinformatics: Where do I start
Sequencing
updated 5 weeks ago by 44k • written 5 weeks ago by ▴ 20
7
votes
7
replies
2.2k
views
Forum: Bioinformatician who was retaliated against after reporting discriminatory harassment - Please help me navigate getting out of the situation I'm in
Career advice
updated 5 weeks ago by ★ 2.5k • written 5 weeks ago by • 0
1
vote
2
replies
416
views
Forum: VCF bulk handling
bcftools SnpEff WGS VEP VCF
5 weeks ago by Javier • 0
16
votes
10
replies
1.2k
views
6 follow
Forum: F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 7 weeks ago by 5.3k • written 7 weeks ago by ★ 2.4k
1
vote
1
reply
254
views
Forum: Can I use this community for software development?
software-development
updated 8 weeks ago by 44k • written 8 weeks ago by • 0
5
votes
2
replies
417
views
Forum: Can questions and answers on biostars be asked only in English? Can't I use Russian for example?
language
updated 8 weeks ago by 54k • written 8 weeks ago by • 0
0
votes
0
replies
200
views
Forum: Field of Study in Computational Biology
Genomics Computational-Biology PhD
updated 9 weeks ago by 44k • written 9 weeks ago by • 0
1
vote
3
replies
524
views
Forum: Development plan for a bioinformatics database developer/coder
developer
9 weeks ago by pixie@bioinfo ★ 1.5k
7
votes
8
replies
828
views
Forum: Bioinformatics career transition
career forum
updated 9 weeks ago by ★ 17k • written 9 weeks ago by ▴ 20
4
votes
2
replies
546
views
Forum: Exploring Amino Acid Patterns in Proteins Through N-gram Analysis
protein CAFA5
10 weeks ago by Anatoly A. ▴ 10
6
votes
6
replies
828
views
Forum: You have 3 days of zero obligations and expansive resources. What are you building?
hypothetical thought-experiment
11 weeks ago by jared.andrews07 ★ 17k
0
votes
0
replies
205
views
Forum: What other data types and pipelines you would like to see from the Genomics Data Commons (GDC)
gdc tcga genomics-data-commons
updated 11 weeks ago by 44k • written 11 weeks ago by ★ 1.2k
0
votes
2
replies
455
views
Forum: What's the state of the art in bacterial whole genome alignment?
whole genome alignment mauve
11 weeks ago by Dave Carlson ★ 1.7k
33
votes
12
replies
1.2k
views
7 follow
Forum: Nextflow and self-made pipelines opinion
nextflow pipeline data-analysis
12 weeks ago by karlaarz ▴ 110
5
votes
4
replies
621
views
Forum: Would reaching out to local labs and startups help with my career transition?
career volunteering
updated 3 months ago by 142k • written 3 months ago by ▴ 10
1
vote
2
replies
409
views
Forum: Exploring Career Reentry: Challenges and opportunities for Women After Maternity and Childcare Breaks
Maternity Career-Gap career-reentry
updated 3 months ago by 82k • written 3 months ago by ▴ 20
8
votes
7
replies
899
views
6 follow
Forum: Tower Server configuration for NGS data analysis
NGS
updated 3 months ago by ▴ 120 • written 3 months ago by • 0
1
vote
0
replies
308
views
Forum: list of bioinformatics tools in cancer genomics
mutations CNA cancer genomics LOH
updated 3 months ago by 162k • written 3 months ago by ★ 1.4k
4
votes
2
replies
411
views
Forum: bulk seq vs. sc-seq
scATAC-seq ATAC-seq scRNAseq RNAseq
updated 3 months ago by ★ 6.1k • written 3 months ago by ▴ 10
8
votes
6
replies
2.9k
views
Forum: Demonstrating Exponential Growth In Pre-School
education
written 10.3 years ago by 10k
0
votes
0
replies
219
views
Forum: tutorial for carrier screening analyses?
carrier-screening
updated 3 months ago by 44k • written 3 months ago by ▴ 20
2
votes
3
replies
464
views
Forum: My Project
Project CLI MD-Simulations Molecular-docking
3 months ago by Ajay A Shettigar • 0
17
votes
11
replies
1.1k
views
Forum: How to overcome the imposter syndrome in bioinformatics?
advice
4 months ago by sil_bioinfo ▴ 40
10
votes
7
replies
2.3k
views
Forum: How to learn bioinformatics?
learning
updated 4 months ago by ★ 1.3k • written 2.0 years ago by ▴ 20
26
votes
27
replies
2.2k
views
10 follow
Forum: Determining computer time required
computer hpc time
updated 4 months ago by ★ 6.1k • written 4 months ago by ▴ 40
7
votes
6
replies
788
views
Forum: where to work as a bioinformatician (NGS/IA)
employment omics ia ngs
updated 4 months ago by 44k • written 4 months ago by ▴ 20
21
votes
12
replies
3.7k
views
Forum: How to Use Biostars Part-3: Formatting Text and Using GitHub Gists
meta how-to documentation
4 months ago by Ram 44k
2
votes
1
reply
472
views
Forum: Cataloguing available spatial transcriptomics tools, then linking them together into a workflow?
cytassist transcriptomics nanostring xenium visium
4 months ago by LauferVA 4.2k
49
votes
20
replies
2.1k
views
10 follow
Forum: First experiences matter
first-time tolerance
updated 4 months ago by ★ 2.6k • written 4 months ago by ★ 27k
4
votes
3
replies
828
views
Forum: How to get started in bioinformatics
students
updated 4 months ago by 44k • written 4 months ago by ▴ 10
2
votes
2
replies
610
views
Forum: Do batches contain biological variations in omics data (RNAseq or scRNAseq)?
combat single-cell omics batch rnaseq
updated 4 months ago by 44k • written 4 months ago by ▴ 40
0
votes
2
replies
3.3k
views
Forum: I’m considering a career transition from software engineering to bioinformatics. Is it a good fit for me?
career
updated 4 months ago by 142k • written 4 months ago by ▴ 10
5
votes
3
replies
654
views
Forum: Binning the rare biosphere
metagenomics soil assembly techniques binning
updated 5 months ago by ★ 27k • written 5 months ago by ▴ 20
6
votes
2
replies
698
views
Forum: how to start Bioinformatics?
basics
updated 5 months ago by ★ 27k • written 5 months ago by • 0
19
votes
5
replies
1.2k
views
6 follow
Forum: What Are The Most Influential Bioinformatics/Computational Biology Papers Of 2023?
genomics snps survey papers
5 months ago by Raony Guimarães ★ 1.4k
4
votes
6
replies
995
views
6 follow
Forum: Bioinformatics: what do you think of it in the next 20 years?
future
updated 5 months ago by 44k • written 5 months ago by • 0
1
vote
1
reply
519
views
Forum: want to work in open source project/project in bioinformatics
project
updated 5 months ago by 44k • written 5 months ago by • 0
3
votes
5
replies
1.1k
views
Forum: Current Trends in Bioinformatics
structural-bioinformatics
updated 5 months ago by 44k • written 6 months ago by • 0
21
votes
11
replies
2.6k
views
6 follow
Forum: Rust bioinformatics projects ideas
projects scRNA-seq RNA-seq Rust
updated 6 months ago by 22k • written 14 months ago by ▴ 150
176
votes
46
replies
19k
views
26 follow
Forum: Crac: Funny And/Or Weird Names For Bioinformatics Tools
humor
6 months ago by Istvan Albert 100k
3
votes
2
replies
650
views
Forum: RNA-Seq data analysis - question
Python RNA-Seq R
updated 6 months ago by ▴ 10 • written 6 months ago by ▴ 40
0
votes
0
replies
395
views
Forum: How to learn bioinformatics online?
self-education education learning
updated 6 months ago by 44k • written 6 months ago by • 0
6
votes
5
replies
2.3k
views
Forum: Maintaning internal or private installation of Biostar or a similar Q&A site
Biostars
6 months ago by tshtatland ▴ 190
7
votes
9
replies
1.5k
views
6 follow
Forum: Mentor for a beginner bioinformatics student
project mentor
6 months ago by UT_FAN ▴ 40
0
votes
0
replies
488
views
Forum: Steps after doublet detection in single cell data
scRNA doublet
7 months ago by ishwarvh • 0
0
votes
0
replies
376
views
Forum: Automated Checks And QA
retrospective
7 months ago by Sasha ▴ 850
7
votes
13
replies
1.4k
views
Forum: How do you validate and verify your pipeline's software updates?
Validation Verification Quality-Assurance
updated 7 months ago by 44k • written 7 months ago by ▴ 10
21
votes
7
replies
12k
views
Forum: Bioinformatics short paper journal resource [software/webapps/database]
journal software webapp
updated 7 months ago by ▴ 100 • written 8.1 years ago by 11k
1,000 results • Page 1 of 20
Recent Votes
A: Fold change - a final explanation
Comment: scRNA-seq quality control
Comment: How to get the ratio of allele counts from GATK derived VCF file?
Setting up Aspera Connect (ascp) on Linux and macOS
Setting up Aspera Connect (ascp) on Linux and macOS
Answer: Genes' fpkm values through cufflink
Answer: Genes' fpkm values through cufflink
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Recent Replies
Answer: How do we do quantification using stringtie merge option for all the merged samp by i.sudbery 19k
If you are asking StringTie to estimate expression, then you need to provide it with BAM or CRAM files of the original reads, not a stringt…
Answer: How to interpret this plotMDS of three disease clusters? by i.sudbery 19k
> 1) How should I interpret this, can I say that there are practically no differences or outliers? You can say that the inter-group vari…
Comment: Ideal PC configurations and operating system for bioinformatics laboratory by emmanouil.a ▴ 120
personaly I see the workstations in long-term ... maybe today you are running only scRNA but having a good workstation tomorrow you can als…
Comment: Z score by Akash D ▴ 40
Alright! Got you...
Comment: Ideal PC configurations and operating system for bioinformatics laboratory by i.sudbery 19k
It very much depends on whether the OP does want/need to do the CellRanger runs locally or not. Doesn't really matter if its only occasion…
Comment: log2 fold change in RNA-seq analysis by Mensur Dlakic ★ 27k
Everything is already explained on this page, just read everything already written above. Specifically, [**this comment**][1]. [1]: htt…
Comment: log2 fold change in RNA-seq analysis by May Ling • 0
Good day everyone, Sorry I found a bit confusing and would appreciate if I could clarify on the calculation. I am using DESeq. My desire…
Answer: GWAS Phenotypes by 韩雨 • 0
In my opinion, the basis of the question is to solve whether the phenotype is regulated by genetics. In the aspect of genetic, the estima…
Answer: Classic threshold for log2 fold change in RNA-seq experiment by May Ling • 0
Good day everyone, Sorry I found a bit confusing and would appreciate if I could clarify on the calculation. I am using DESeq. My desire…
Comment: some error in building kraken2 database by ahmet • 0
Were you able to fix this issue? I'm also trying to add external species to krakendb
Comment: Z score by ATpoint 82k
logFCs are a bit difficult to cluster I think, because fold changes without stats attached to it are meaningless. They can be very big, due…
Comment: Z score by Akash D ▴ 40
Ok. However, if i perform it on log2FC, there wont be any control samples visible in my heatmap. Your answer is perfect.
Comment: Z score by ATpoint 82k
You can apply Z-scores on whatever you want, but it must make sense and depends on what you want to do. What is the context? See for exampl…
Answer: Z score in RNAseq by Akash D ▴ 40
Can Z score be applied on log2FC and then visualized? Alternatively, Can log2FC be converted to Z scores and then visualized as heatmap?
Comment: Ideal PC configurations and operating system for bioinformatics laboratory by ATpoint 82k
Just for comparison, I deal with scRNA-seq daily and my workstation is a 12700k i7 with 128GB RAM and even this is actually more than I nee…
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