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0 votes
11 replies
2.2K views
Current size of blast nr database as FASTA file
fasta blast
updated by lieven.sterck 1.6K • | written by pahurkar.arjun 0
0 votes
5 replies
1.6K views
SRA FTP Upload Issue
ftp sra ncbi
updated by Jesse 94 • | written by saamhasan55 3
2 votes
4 replies
1.3K views
How to calculate total and average read counts from paired-end and singleton FASTQ files?
metagenomics
updated by GenoMax 16K • | written by Sachin 2
0 votes
1 reply
695 views
New computer purchase
linux
updated by GenoMax 16K • | written by Edward 0
0 votes
0 replies
1.3K views
News: Python for Biological Data Exploration & Visualization (PYBD01)
python
updated by GenoMax 16K • | written by oliverhooker 14
0 votes
0 replies
1.1K views
News: Introduction to Machine Learning (IMLR04)
learning r machine
updated by GenoMax 16K • | written by oliverhooker 14
0 votes
2 replies
1.0K views
When absolute protein quantification isn't possible, what anaylses work on ranked expression?
dia-ms proteomics
updated by Ram 4.5K • | written by Ali 0
0 votes
0 replies
669 views
lncRNA and mRNA correlation analysis results
correlation lncrna ncrna coexpression mrna
written by Zeynep 0
0 votes
2 replies
2.4K views
Scanpy Dotplot / Matplotlib Help
matplotlib dotplot python scanpy
written by cthangav 11
0 votes
2 replies
1.5K views
Help regarding quality control of 10X run?
10x nuclei qc scnuc
updated by jared.andrews07 1.9K • | written by Pratik 110
0 votes
1 reply
1.1K views
PLINK 1.9 and ADMIXTURE 1.3.0 Help with renaming
admixture error plink debugging coding
updated by Corentin 66 • | written by Jens 0
0 votes
0 replies
576 views
salmon alevin multimapping reads/ tier3 genes
salmon alevin
written by nmannda148 0
0 votes
3 replies
1.3K views
How can I confidently determine if my genome sequence Is truly Anaplasma marginale?
anaplasma genomics marginale omics
updated by Ram 4.5K • | written by ashiqullah024 0
0 votes
13 replies
4.0K views
Trouble with Standalone Blast Tool (running for 2 hours and no output)
genome fasta blastx blast
updated by Ram 4.5K • | written by pahurkar.arjun 0
0 votes
0 replies
830 views
Forum: Seeking unique bioinformatics angle for wildlife-livestock AMR gut microbiome study (PhD proposal help)
antimicrobial-resistance metagenomics microbiome amr
updated by Ram 4.5K • | written by Saeed 0
0 votes
6 replies
1.7K views
Running Arriba on galaxy
galaxy
written by Abdelrahman 0
0 votes
4 replies
1.6K views
how to extract centromeric regions from human assemblies/FASTA
centromeres fasta
updated by colindaven 844 • | written by Matteo Ungaro 16
0 votes
1 reply
783 views
Changes cgMLST type of Neisseria gonorrhoeae
cgmlst neisseria gonorrhoeae
updated by GenoMax 16K • | written by nahid.islam.zahid 0
0 votes
0 replies
712 views
Appropiate normalization method for OTU table built from MAGs abundances estimations?
mag normalization otus metagenomics
written by v.berriosfarias 14
0 votes
1 reply
785 views
scRNA-seq annotation with Revsc
epithelialcells intestinal revivalstemcell
updated by Arup Ghosh 402 • | written by Farha 0
1 vote
3 replies
1.1K views
dbSNP: left-shift vs right-shift of indels
dbsnp
updated by GenoMax 16K • | written by Matthew 1
0 votes
3 replies
941 views
duplicate reads in RNAseq datasets
rnaseq r
updated by dsull 781 • | written by Bioinfonext 48
0 votes
0 replies
788 views
Tools to model DEG/pathways in (fast-dividing) CANCER cell lines across timepoints? (in bulk RNA-seq)
deg rna-seq bulk
written by txema.heredia 31
3 votes
5 replies
8.0K views
Combine a directory of GVCF files with gatk CombineGVCFs
gatk combinegvcfs gvcf
updated by DareDevil 448 • | written by michael.flower.14 21
0 votes
4 replies
1.9K views
UMI introduction and deduplication with SMARTer-Seq
rnaseq sequencing umi rna
updated by i.sudbery 2.3K • | written by rayanelkholdi 0
0 votes
0 replies
1.1K views
News: AI for Genomics: From CNNs and LSTMs to Transformers (Online, 9–11 September)
deeplearning lstms ai cnns machinelearning
written by Physalia-courses 281
0 votes
1 reply
731 views
Merged seurat objects, clustering problem, help please
harmony seurat clustering scrna
updated by ATpoint 9.1K • | written by rmorabar 0
0 votes
4 replies
1.7K views
Linkage disequilibrium estimation in unphased data?
correlation wes pearson ld kendall
written by Joel Wallenius 22
0 votes
6 replies
1.9K views
Why do Sox9 and Amh, which are Sertoli cell markers, show expression in the overall cell population?
analysis scrna-seq
written by Bio 1
0 votes
1 reply
880 views
ONT bulk PCR cDNA protocol
bulk ont pcr adapter umi
updated by GenoMax 16K • | written by Sowmya 0
1 vote
7 replies
2.8K views
Fungal Annotation Comparison
annotation rnaseq fungus genome_assembly funannotate
updated by SanjidaR_NCBI 7 • | written by SomeOne 33
0 votes
3 replies
1.0K views
VCF Header malformation
update vcf dictionary mismache header
updated by Jeremy Leipzig 2.3K • | written by Mahdi&Christ 2
0 votes
0 replies
1.2K views
News: Unlock the Power of Python for Biological Research: PYBD01
python
updated by GenoMax 16K • | written by oliverhooker 14
0 votes
6 replies
1.8K views
STAR alignment to small fasta file failing
seq star alignment rna
written by campbio 0
0 votes
0 replies
1.4K views
Job: Postdoctoral Researcher (DFG‑funded): AI & Ageing Biology
bioinformatics longevity aging ai open-source
written by Anton 14
0 votes
3 replies
1.7K views
ONT Methylation calling
modifications nanopore ont epigenetic sequencing
updated by colindaven 844 • | written by Franci 0
0 votes
3 replies
1.6K views
picard MarkDuplicates READ_PAIRS_EXAMINED much lower than the number of read pairs successfully mapped by STAR
star removal rna-seq picard duplicate
updated by GenoMax 16K • | written by maria 0
1 vote
6 replies
4.3K views
Which threshold to be put in the MAPQ when doing mpileup of BCF tools on a STAR-generated BAM file ?
rnaseq star
updated by Titus 91 • | written by mohsamir2016 3
1 vote
1 reply
956 views
Mendelian Randomization, Liftover
mendelian-randomization liftover genetics
updated by gianmarcoabbadessa 0 • | written by lag 1
0 votes
4 replies
1.8K views
Identifying primers in rna-seq data
cutadapt transcriptomics fastqc rna-seq primers
updated by ATpoint 9.1K • | written by mtsrn 0
0 votes
6 replies
1.4K views
Plant transcriptome analysis and annotation
plants srna-seq adnnotation analysis expression
written by martta95 1
0 votes
1 reply
873 views
Is Dorado, nanopolish, and xpore compatible with each other?
nanopolish ont dorado samtools xpore
updated by GenoMax 16K • | written by ward.alafef 0
0 votes
2 replies
1.2K views
Average Nucleotide Identity for bins
ani bin data shotgun
written by shevch2009 2
0 votes
0 replies
1.3K views
News: Introduction to Python for Biologists (IPYB01)
python
updated by GenoMax 16K • | written by oliverhooker 14
3 votes
0 replies
824 views
Tool: CATS: reference-free and reference-based transcriptome assembly quality assessment
tool rna-seq transcriptomics transcriptome
written by Kristian 3
0 votes
1 reply
626 views
Systematic review and Metanalysis
metanalysis systematic-review
updated by Ram 4.5K • | written by Sara 0
0 votes
0 replies
615 views
Novel Junctions from SJ.out.tab
junctions rstudio novel star
written by SpliceAndScript 0
0 votes
0 replies
740 views
Getting wrong scissor results while integrating bulk and single cell data
bulk intrgration single scissor cell tnbc
written by TNF_alpha 0
4 votes
14 replies
68K views
Tools to view fastq file
fastq
updated by GenoMax 16K • | written by Roden Luo 4
0 votes
4 replies
1.6K views
how extract CpG methylation calls from nanopore long sequencing bam file ?
methylation
written by reziyamaimaiti0331 0
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