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0 votes
2 replies
2.5K views
News: Hands-On Workshop - Next-Generation Sequencing Data Analysis: A Practical Introduction (September 10-12, 2025 in Berlin, Germany)
workshop rna-seq illumina variantcalling dna-seq
written by ecSeq Bioinformatics • ecSeq Bioinformatics
0 votes
0 replies
887 views
Is it possible to perform XP-EHH and iHS on targeted sequencing data without a .map file?
ngs ihs analysis selection sequencing
written by slzr_ • slzr_
0 votes
0 replies
414 views
Job: Scientist Bioinformatics at BASF | Nunhems (near Eindhoven , The Netherlands)
basf plants
written by WS_jobs • WS_jobs
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2 replies
1.1K views
Discrepancy in RNA editing calls between STAR and Subjunc aligners using JACUSA2
jacusa2 rnaseq subjunc star rsubread
written by thomas.heigl.ibk • thomas.heigl.ibk
1 vote
1 reply
830 views
RNA-Seq paired-end datasets
paired-end rna-seq bioinformatic
updated by Matthias Zepper Matthias Zepper • written by AIMAR • AIMAR
0 votes
0 replies
1.1K views
News: Course: Introduction to CRISPR
genomeediting crispr evolution
written by Physalia-courses Physalia-courses
0 votes
3 replies
1.5K views
Best strategy for comparing cell-type composition between disease and healthy groups in single-cell RNA-seq data
single_cell
updated by ATpoint ATpoint • written by 城玮 • 城玮
0 votes
4 replies
1.5K views
RNA-seq assigned alignments
paired-end star rna-seq mapping single-end
updated by jaro.slamecka jaro.slamecka • written by Manko47 • Manko47
0 votes
1 reply
866 views
Obtain Common SNPS for Mouse Genome
mouse snps
updated by GenoMax GenoMax • written by joe_genome • joe_genome
0 votes
3 replies
2.4K views
How do you clean GEO metadata before downstream analysis?
geo
updated by yura.grabovska • yura.grabovska • written by Alba • Alba
1 vote
1 reply
2.3K views
Seeking high-quality curated metabolic models with time-resolved flux data for sonification project
metabolism sonification networks
updated by Mensur Dlakic Mensur Dlakic • written by omereilam1 • omereilam1
0 votes
0 replies
585 views
Standard way to represent represent indel (insertion or deletion) alleles within a haplotype
haplotypes indels haploview
written by Mwangana • Mwangana
0 votes
1 reply
3.6K views
lovis4u Configuration
graph lovis4u genomics
updated by GenoMax GenoMax • written by NIkita • NIkita
0 votes
1 reply
2.2K views
Tool: Tool to clean GEO metadata
geo metadata
updated by yura.grabovska • yura.grabovska • written by Alba • Alba
1 vote
2 replies
4.0K views
WGBS Analysis with 1 control sample and 6 disease samples
one wgbs sample
updated by ATpoint ATpoint • written by Cassandra • Cassandra
0 votes
2 replies
4.1K views
How to extract gene list from GEO dataset in R
coding beginner question r
updated by Shohel • Shohel • written by decorus • decorus
0 votes
6 replies
4.5K views
RNA seq strandedness (reverse-strand)
seq reverse-stranded strand rna
written by ZuelTech • ZuelTech
0 votes
0 replies
475 views
TFs and enrichment analysis for TRAP sequencing
trap
written by Genesis • Genesis
0 votes
2 replies
3.7K views
Extract track data from "search by region" feature on ENCODE
encode
written by AlienPumpkinPie • AlienPumpkinPie
1 vote
8 replies
5.1K views
Weird p-value distribution on edgeR results
edger pvalue-distribution gene-expression
updated by dariober dariober • written by Guillermo • Guillermo
0 votes
4 replies
4.0K views
Draw GO tree for some GO ids with coloring
enrichment go
updated by Istvan Albert Istvan Albert • written by boczniak767 boczniak767
0 votes
4 replies
2.1K views
Problem with Mirdeep2 and Randfold output
mirdeep2 randfold
updated by Jasim • Jasim • written by otieno43 • otieno43
0 votes
1 reply
1.0K views
calculating coverage of gene in my metagenomes with samtools
samtools depth metagenome coverage
updated by Pierre Lindenbaum Pierre Lindenbaum • written by lintonf • lintonf
0 votes
1 reply
996 views
how to merge gbz files
vg
updated by Jouni Sirén Jouni Sirén • written by lushjia • lushjia
0 votes
1 reply
711 views
plotting read length distribution of Single End data
read_length_distribution single_end_sequencing wgs
written by Meghan.T • Meghan.T
0 votes
1 reply
809 views
Preranked GSEA permutation
gsea
updated by ATpoint ATpoint • written by as823jk • as823jk
0 votes
2 replies
3.8K views
why bwa-mem only return one read with pair-read end
sequencing bwa-mem
updated by rfran010 • rfran010 • written by QX • QX
0 votes
2 replies
798 views
Identification problem of IDE-8 cell on culture with inverted microscope
ide-8 offtopic
updated by i.sudbery i.sudbery • written by Prashanta • Prashanta
0 votes
2 replies
878 views
Identifying RBP motifs overlapping exons
motifs bedtools rbp
updated by GenoMax GenoMax • written by RK • RK
0 votes
0 replies
1.2K views
News: Upcoming NGS Data Analysis Courses 2025
singlecell rnaseq epigenomics illumina dnaseq
written by ecSeq Bioinformatics • ecSeq Bioinformatics
0 votes
1 reply
756 views
How to match clinical information with expression data in R
r
updated by Arup Ghosh Arup Ghosh • written by py3296746920 • py3296746920
1 vote
1 reply
925 views
Identify SNP, Indel variants from a List of FASTA sequence
snp
updated by Mark Mark • written by Trinh • Trinh
1 vote
20 replies
5.5K views
Which Reference Hg38 or T2T?? Both??
host removal
updated by Business • Business • written by Jon • Jon
0 votes
2 replies
966 views
Can I transduce HEK293T with Cas9 lentivirus followed by transfection with sgRNAs?
lentivirus transduction crispr transfection offtopic
updated by jared.andrews07 jared.andrews07 • written by louisflower1999 • louisflower1999
0 votes
4 replies
1.2K views
Mitochondrial annotation tool for algae
barcoding annotation
written by firefox91 • firefox91
0 votes
2 replies
2.5K views
Identifying gene outliers in a genome using composition analysis.
rscu composition gc outliers
updated by GenoMax GenoMax • written by Shakunthala Natarajan • Shakunthala Natarajan
0 votes
5 replies
1.3K views
Mapping to a template sequece
sequencing
written by QX • QX
0 votes
2 replies
1.1K views
quality control for 16s metagenome reads
quality metagneome control 16s fastp
updated by GenoMax GenoMax • written by m90 • m90
0 votes
3 replies
1.2K views
Loss of strandedness in the Illumina Strandedness Total RNA protocol
illumina rna-seq reads stranded
written by Anjan Anjan
0 votes
1 reply
1.3K views
Help using ShinyCell2 and Seurat v5 object.
shinycell seurat shiny shinycell2 scrna
updated by antonioggsousa antonioggsousa • written by Jamie • Jamie
0 votes
0 replies
1.1K views
News: Machine Learning for Multi-Omics Integration at the University of Barcelona– Dec 2025
machine-learning sulti-omics depp-learning data-integration bigdata
written by Physalia-courses Physalia-courses
0 votes
0 replies
694 views
Compare alternative splicing patterns between human and mouse?
orthologs rna-seq splicing alternative
written by aboll • aboll
0 votes
6 replies
1.7K views
Trouble getting representatives from clustered_nr database
database blast
written by Adolfo • Adolfo
0 votes
0 replies
583 views
FindTransferAnchors (reference prediction) vs Manual Annotations
seurat
written by reddyornah • reddyornah
0 votes
4 replies
1.9K views
NCBI Datasets CLI Question
ncbi-datasets
written by Bjorn • Bjorn
0 votes
0 replies
541 views
Incomplete assembly in HPRC dataset
pangenome hprc reference
written by ohell • ohell
2 votes
3 replies
1.5K views
Joint Calling for Large Germline WGS Cohort
ngs ram cohort genomics wgs
updated by Jeremy Leipzig Jeremy Leipzig • written by j.k3096 • j.k3096
0 votes
4 replies
1.2K views
how shoud the phred score be intepreted?
sequencing
updated by GenoMax GenoMax • written by QX • QX
0 votes
3 replies
1.1K views
Inherited pipeline uses RNA-seq variant calls for WASP filtering—shouldn’t it be external SNPs?
wasp
updated by i.sudbery i.sudbery • written by jonas.andersson • jonas.andersson
0 votes
1 reply
974 views
WGCNA analysis for Proteomics data
proteomics wgcna
updated by Vinesh • Vinesh • written by Sumit Paliwal • Sumit Paliwal
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