Hey everyone, I'm working on annotating an scRNA-seq dataset from intestinal epithelial cells, focusing specifically on the epithelium and trying to identify the Revival Stem Cell (RevSC) population. I'm seeing something odd though—when I look at a violin plot of RevSC expression across healing stages, the "None" stage (i.e. non-injured) shows unexpectedly high RevSC expression. This contradicts published literature, which suggests RevSCs are either not present or minimally expressed under normal conditions.

Has anyone else encountered this? Could this be an annotation issue, or should I revisit my marker selection or filtering strategy?

Any insights or suggestions are appreciated!