ONT bulk PCR cDNA protocol

Is UMI ligation typically used in bulk ONT PCR cDNA sequencing? If not, how is deduplication handled given the risk of false positives from full-length transcript duplicates?

bulk ont pcr adapter umi

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updated 1.0 years ago by GenoMax

• written 1.0 years ago by Sowmya •

1 answer

https://nanoporetech.com/document/pcr-cdna-sequencing-v14-sqk-pcs114 cDNA protocol incorporates UMI's.

It is for https://store.nanoporetech.com/us/cdna-pcr-sequencing-kit-v14.html cDNA-PCR kit.

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written 1.0 years ago by GenoMax

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