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1,000 results • Page
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6
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7
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552
views
7 follow
Forum:
Ideal PC configurations and operating system for bioinformatics laboratory
PC
updated 1 hour ago by
ATpoint
82k • written 29 days ago by
Estevão
▴ 10
0
votes
0
replies
199
views
Forum:
Improvement on automatic annotation of single-cell RNA-seq data from the literature using LLM
annotation
scRNA
llm
4 weeks ago by
yxwucq
• 0
5
votes
2
replies
388
views
Forum:
Bioinformatics: Where do I start
Sequencing
updated 5 weeks ago by
Ram
44k • written 5 weeks ago by
Adi
▴ 20
7
votes
7
replies
2.2k
views
Forum:
Bioinformatician who was retaliated against after reporting discriminatory harassment - Please help me navigate getting out of the situation I'm in
Career
advice
updated 5 weeks ago by
Dunois
★ 2.5k • written 5 weeks ago by
AdviceSeeker
• 0
1
vote
2
replies
416
views
Forum:
VCF bulk handling
bcftools
SnpEff
WGS
VEP
VCF
5 weeks ago by
Javier
• 0
16
votes
10
replies
1.2k
views
6 follow
Forum:
F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 7 weeks ago by
Madelaine Gogol
5.3k • written 7 weeks ago by
Ann
★ 2.4k
1
vote
1
reply
254
views
Forum:
Can I use this community for software development?
software-development
updated 8 weeks ago by
Ram
44k • written 8 weeks ago by
avpuzanov
• 0
5
votes
2
replies
417
views
Forum:
Can questions and answers on biostars be asked only in English? Can't I use Russian for example?
language
updated 8 weeks ago by
Michael
54k • written 8 weeks ago by
avpuzanov
• 0
0
votes
0
replies
200
views
Forum:
Field of Study in Computational Biology
Genomics
Computational-Biology
PhD
updated 9 weeks ago by
Ram
44k • written 9 weeks ago by
Mohammed
• 0
1
vote
3
replies
524
views
Forum:
Development plan for a bioinformatics database developer/coder
developer
9 weeks ago by
pixie@bioinfo
★ 1.5k
7
votes
8
replies
827
views
Forum:
Bioinformatics career transition
career
forum
updated 9 weeks ago by
jared.andrews07
★ 17k • written 9 weeks ago by
jevill17
▴ 20
4
votes
2
replies
546
views
Forum:
Exploring Amino Acid Patterns in Proteins Through N-gram Analysis
protein
CAFA5
10 weeks ago by
Anatoly A.
▴ 10
6
votes
6
replies
827
views
Forum:
You have 3 days of zero obligations and expansive resources. What are you building?
hypothetical
thought-experiment
11 weeks ago by
jared.andrews07
★ 17k
0
votes
0
replies
205
views
Forum:
What other data types and pipelines you would like to see from the Genomics Data Commons (GDC)
gdc
tcga
genomics-data-commons
updated 11 weeks ago by
Ram
44k • written 11 weeks ago by
Zhenyu Zhang
★ 1.2k
0
votes
2
replies
455
views
Forum:
What's the state of the art in bacterial whole genome alignment?
whole
genome
alignment
mauve
11 weeks ago by
Dave Carlson
★ 1.7k
33
votes
12
replies
1.2k
views
7 follow
Forum:
Nextflow and self-made pipelines opinion
nextflow
pipeline
data-analysis
11 weeks ago by
karlaarz
▴ 110
5
votes
4
replies
621
views
Forum:
Would reaching out to local labs and startups help with my career transition?
career
volunteering
updated 3 months ago by
GenoMax
142k • written 3 months ago by
Mango
▴ 10
1
vote
2
replies
409
views
Forum:
Exploring Career Reentry: Challenges and opportunities for Women After Maternity and Childcare Breaks
Maternity
Career-Gap
career-reentry
updated 3 months ago by
ATpoint
82k • written 3 months ago by
ashaneev07
▴ 20
8
votes
7
replies
898
views
6 follow
Forum:
Tower Server configuration for NGS data analysis
NGS
updated 3 months ago by
emmanouil.a
▴ 120 • written 3 months ago by
harasharan
• 0
1
vote
0
replies
308
views
Forum:
list of bioinformatics tools in cancer genomics
mutations
CNA
cancer
genomics
LOH
updated 3 months ago by
Pierre Lindenbaum
162k • written 3 months ago by
Bogdan
★ 1.4k
4
votes
2
replies
411
views
Forum:
bulk seq vs. sc-seq
scATAC-seq
ATAC-seq
scRNAseq
RNAseq
updated 3 months ago by
dsull
★ 6.1k • written 3 months ago by
SebKittelmann
▴ 10
8
votes
6
replies
2.9k
views
Forum:
Demonstrating Exponential Growth In Pre-School
education
written 10.3 years ago by
Asaf
10k
0
votes
0
replies
219
views
Forum:
tutorial for carrier screening analyses?
carrier-screening
updated 3 months ago by
Ram
44k • written 3 months ago by
Akos
▴ 20
2
votes
3
replies
464
views
Forum:
My Project
Project
CLI
MD-Simulations
Molecular-docking
3 months ago by
Ajay A Shettigar
• 0
17
votes
11
replies
1.1k
views
Forum:
How to overcome the imposter syndrome in bioinformatics?
advice
4 months ago by
sil_bioinfo
▴ 40
10
votes
7
replies
2.3k
views
Forum:
How to learn bioinformatics?
learning
updated 4 months ago by
Samuel Lampa
★ 1.3k • written 2.0 years ago by
sakichwan
▴ 20
26
votes
27
replies
2.2k
views
10 follow
Forum:
Determining computer time required
computer
hpc
time
updated 4 months ago by
dsull
★ 6.1k • written 4 months ago by
Rozita
▴ 40
7
votes
6
replies
788
views
Forum:
where to work as a bioinformatician (NGS/IA)
employment
omics
ia
ngs
updated 4 months ago by
Ram
44k • written 4 months ago by
Petur
▴ 20
21
votes
12
replies
3.7k
views
Forum:
How to Use Biostars Part-3: Formatting Text and Using GitHub Gists
meta
how-to
documentation
4 months ago by
Ram
44k
2
votes
1
reply
471
views
Forum:
Cataloguing available spatial transcriptomics tools, then linking them together into a workflow?
cytassist
transcriptomics
nanostring
xenium
visium
4 months ago by
LauferVA
4.2k
49
votes
20
replies
2.1k
views
10 follow
Forum:
First experiences matter
first-time
tolerance
updated 4 months ago by
Michael Love
★ 2.6k • written 4 months ago by
Mensur Dlakic
★ 27k
4
votes
3
replies
828
views
Forum:
How to get started in bioinformatics
students
updated 4 months ago by
Ram
44k • written 4 months ago by
Soidihane
▴ 10
2
votes
2
replies
610
views
Forum:
Do batches contain biological variations in omics data (RNAseq or scRNAseq)?
combat
single-cell
omics
batch
rnaseq
updated 4 months ago by
Ram
44k • written 4 months ago by
cwwong13
▴ 40
0
votes
2
replies
3.3k
views
Forum:
I’m considering a career transition from software engineering to bioinformatics. Is it a good fit for me?
career
updated 4 months ago by
GenoMax
142k • written 4 months ago by
Mango
▴ 10
5
votes
3
replies
654
views
Forum:
Binning the rare biosphere
metagenomics
soil
assembly
techniques
binning
updated 5 months ago by
Mensur Dlakic
★ 27k • written 5 months ago by
Konstantin
▴ 20
6
votes
2
replies
697
views
Forum:
how to start Bioinformatics?
basics
updated 5 months ago by
Mensur Dlakic
★ 27k • written 5 months ago by
sajibsuvo70
• 0
19
votes
5
replies
1.2k
views
6 follow
Forum:
What Are The Most Influential Bioinformatics/Computational Biology Papers Of 2023?
genomics
snps
survey
papers
5 months ago by
Raony Guimarães
★ 1.4k
4
votes
6
replies
995
views
6 follow
Forum:
Bioinformatics: what do you think of it in the next 20 years?
future
updated 5 months ago by
Ram
44k • written 5 months ago by
Daniel
• 0
1
vote
1
reply
519
views
Forum:
want to work in open source project/project in bioinformatics
project
updated 5 months ago by
Ram
44k • written 5 months ago by
Afeef
• 0
3
votes
5
replies
1.1k
views
Forum:
Current Trends in Bioinformatics
structural-bioinformatics
updated 5 months ago by
Ram
44k • written 6 months ago by
Paulo
• 0
21
votes
11
replies
2.6k
views
6 follow
Forum:
Rust bioinformatics projects ideas
projects
scRNA-seq
RNA-seq
Rust
updated 6 months ago by
Jeremy Leipzig
22k • written 14 months ago by
bompipi95
▴ 150
176
votes
46
replies
19k
views
26 follow
Forum:
Crac: Funny And/Or Weird Names For Bioinformatics Tools
humor
6 months ago by
Istvan Albert
100k
3
votes
2
replies
650
views
Forum:
RNA-Seq data analysis - question
Python
RNA-Seq
R
updated 6 months ago by
fturner
▴ 10 • written 6 months ago by
sil_bioinfo
▴ 40
0
votes
0
replies
395
views
Forum:
How to learn bioinformatics online?
self-education
education
learning
updated 6 months ago by
Ram
44k • written 6 months ago by
Brian Rey | BioLearn
• 0
6
votes
5
replies
2.3k
views
Forum:
Maintaning internal or private installation of Biostar or a similar Q&A site
Biostars
6 months ago by
tshtatland
▴ 190
7
votes
9
replies
1.5k
views
6 follow
Forum:
Mentor for a beginner bioinformatics student
project
mentor
6 months ago by
UT_FAN
▴ 40
0
votes
0
replies
487
views
Forum:
Steps after doublet detection in single cell data
scRNA
doublet
7 months ago by
ishwarvh
• 0
0
votes
0
replies
376
views
Forum:
Automated Checks And QA
retrospective
7 months ago by
Sasha
▴ 850
7
votes
13
replies
1.4k
views
Forum:
How do you validate and verify your pipeline's software updates?
Validation
Verification
Quality-Assurance
updated 7 months ago by
Ram
44k • written 7 months ago by
kieran
▴ 10
21
votes
7
replies
12k
views
Forum:
Bioinformatics short paper journal resource [software/webapps/database]
journal
software
webapp
updated 7 months ago by
Gennady Khvorykh
▴ 100 • written 8.1 years ago by
Sukhi Singh
11k
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Comment: scRNA-seq quality control
Comment: How to get the ratio of allele counts from GATK derived VCF file?
Setting up Aspera Connect (ascp) on Linux and macOS
Setting up Aspera Connect (ascp) on Linux and macOS
Answer: Genes' fpkm values through cufflink
Answer: Genes' fpkm values through cufflink
Genes' fpkm values through cufflink
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Recent Replies
Comment: Z score
by
Akash D
▴ 40
Ok. However, if i perform it on log2FC, there wont be any control samples visible in my heatmap.
Comment: Z score
by
ATpoint
82k
You can apply Z-scores on whatever you want, but it must make sense and depends on what you want to do. What is the context? See for exampl…
Answer: Z score in RNAseq
by
Akash D
▴ 40
Can Z score be applied on log2FC and then visualized? Alternatively, Can log2FC be converted to Z scores and then visualized as heatmap?
Comment: Ideal PC configurations and operating system for bioinformatics laboratory
by
ATpoint
82k
Just for comparison, I deal with scRNA-seq daily and my workstation is a 12700k i7 with 128GB RAM and even this is actually more than I nee…
Answer: Ideal PC configurations and operating system for bioinformatics laboratory
by
colindaven
6.4k
- As others have said, 1-2 workstations are probably the way to go. - A Linux OS like Ubuntu is probably easiest and has best docs - RA…
Comment: How to get the ratio of allele counts from GATK derived VCF file?
by
anikcropscience
▴ 230
Ok, thank you for the reply. I will check out the DP field then.
Comment: Alternatives To Liftover
by
Thanujay S
• 0
Hey! Would you consider building an API for the same? Is that something you have planned?
Answer: Ideal PC configurations and operating system for bioinformatics laboratory
by
emmanouil.a
▴ 120
20k are good money for some workstations ... probably you need to run more samples at the same time ... for some 1000s you could take a wor…
Comment: samtools write-index
by
LucisTheFather
• 0
``` -o alignment.sorted.bam##idx##alignment.sorted.bam.bai ``` This portion is to get .bai index, instead of .csi indexing. Although Idk th…
Comment: samtools write-index
by
LucisTheFather
• 0
Really appreciate your help Pierre! I am a little bit confused btw. do you mean this: ``` rm -vf alignment.sorted.bam.bai minimap2 -ax map-…
Comment: samtools write-index
by
LucisTheFather
• 0
Yeah, I did multiple split tests of my pipeline (just subset 400k rows of my fastq and run through my pipeline for quick tests) and none of…
Comment: Which 1000 genomes 30x files should I use for imputation?
by
David-walson
• 0
Hi there. Have you found any suggestions? I have alos been working on the 1KGP high coverage data, but i have no idea about how to use it p…
Comment: MAUVE: No gene annotations in Genbank file alignments
by
pramach1
▴ 40
The same problem persists for me too. Running progressiveMauve on command line on bunch of gbk files. Mauve itself runs fine and I do see t…
Answer: Error when looping over multiple columns in a data frame in R
by
Jeremy
▴ 910
First of all, you should use "tag.healthy" and "methods" instead of "tag.health" and "method". Also, optimal.cutpoints() wants X to be eit…
Comment: Mouse or Rat Gene Expression Data Similar to GTEx
by
GenoMax
142k
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