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0 votes
0 replies
488 views
how to make a recombination map using a family of a diploid bee "mom" and 5 haploid "sons"
sequencing recombination-map
updated by Ram Ram • written by dovasuttner • dovasuttner
2 votes
0 replies
1.0K views
Job: Assistant, Associate, or Full Project Scientist in Cell & Developmental Biology UC San Diego
biology academia ucsd
written by UCSDBioSci • UCSDBioSci
1 vote
3 replies
1.6K views
Snakemake - Missing output files
snakemake
updated by Ram Ram • written by am29 • am29
0 votes
6 replies
1.8K views
Best practice for gene ontology/pathway/geneset enrichment in 10x data
r seurat scrna
updated by jared.andrews07 jared.andrews07 • written by yura.grabovska • yura.grabovska
0 votes
2 replies
3.3K views
Plink HWE QC question
hwe plink p-value
updated by chrchang523 chrchang523 • written by janny.lau • janny.lau
0 votes
0 replies
630 views
Can non-integer data be used as input for Monocle3?
pseudotime scrnaseq trajectory-analysis monocle3
updated by Ram Ram • written by annadv • annadv
0 votes
2 replies
867 views
multiple sequence alignmet
msa
updated by Mensur Dlakic Mensur Dlakic • written by harry • harry
0 votes
4 replies
1.4K views
Different result by bowtie2
bowtie2
updated by GenoMax GenoMax • written by daffodil • daffodil
0 votes
0 replies
565 views
Strand inconsistency between Long read tool and minimap2?
minimap2
written by shinyjj • shinyjj
0 votes
1 reply
2.6K views
Problems with using Phobius
phobius fasta sequence
updated by Ram Ram • written by logleg92029 • logleg92029
0 votes
5 replies
2.0K views
Some questions about motif enrichment in Homer
motif-enrichment methylation dna
updated by Srinka • Srinka • written by diqixiaoyaoer • diqixiaoyaoer
0 votes
1 reply
695 views
Genomics Features Extraction (Promoter, 5'UTR, 3'UTR, Intergenic, exnoic, intronic etc)
genomics biology
updated by Ram Ram • written by shabbas12 • shabbas12
0 votes
0 replies
633 views
Cuffcompare annotation and class code
rna-seq cuffcompare
written by ZZOYEA • ZZOYEA
0 votes
0 replies
533 views
Analyzing multiple microRNA sequencing GEO datasets at the same time
geo-database rna-seq metavolcano microrna
updated by Ram Ram • written by Alana • Alana
0 votes
1 reply
1.1K views
How can you determine the mutation rate of mitochondrial DNA for your (Grasshopper) species?
pacbio numts mtdna genomics
updated by Brian Bushnell Brian Bushnell • written by hashim.rana11 • hashim.rana11
0 votes
1 reply
1.2K views
Tandem Mass Tag (TMT) proteomics data analysis
tmt tandem-mass-tag proteomics
updated by Ram Ram • written by F • F
0 votes
1 reply
687 views
Conflict packages R
r
updated by Ram Ram • written by marco.barr • marco.barr
0 votes
4 replies
4.1K views
Trimmomatic or Cutadapt not Trimming my Adapters even though I see them clearly in the Fastq-File
rna-seq cutadapt trimmomatic
updated by Brian Bushnell Brian Bushnell • written by obsto123 • obsto123
0 votes
6 replies
2.1K views
Understanding bedtools coverage for datasets with in-dels.
ont bedtools mapping reads coverage
written by Shakunthala Natarajan • Shakunthala Natarajan
0 votes
4 replies
1.9K views
De novo sequencing?
de-novo-sequencing
updated by Ram Ram • written by Abdul Rafay Khan Abdul Rafay Khan
1 vote
10 replies
3.4K views
Find all sequences with adapters without trimming them off
cutadapt fastq
updated by Brian Bushnell Brian Bushnell • written by Freyda • Freyda
8 votes
4 replies
2.2K views
Forum: Awesome awesomeness bioinformatics
bioinformatics awesome
written by Juke34 Juke34
0 votes
0 replies
566 views
Pangenome Construction
pangenome chromosome
written by bioinfo223 • bioinfo223
0 votes
0 replies
579 views
Plotting admixture output with circular barplot using membercoeff.circos
bitev2 membercoeff admixture
written by PKW • PKW
0 votes
1 reply
1.4K views
STAR aligner STAR: bgzf.c:158: bgzf_open: Assertion `compressBound(BGZF_BLOCK_SIZE) < BGZF_MAX_BLOCK_SIZE' failed.
star aligner bgzf
updated by Ram Ram • written by tedwinkent • tedwinkent
0 votes
1 reply
1.1K views
Convert CRAM to BAM in pysam
python pysam bam cram
updated by Brian Bushnell Brian Bushnell • written by sviatoslav.kendall sviatoslav.kendall
1 vote
12 replies
3.5K views
comparison between a selected genes of a new strain and the same genes of the reference genome
strain genome pipeline
written by avinci1 • avinci1
0 votes
0 replies
414 views
Compensation for SSC-A/CD3-PerCP?
compensation flowjo
written by ysun • ysun
0 votes
3 replies
1.4K views
Looking for some guidance regarding motif ontology
atac-seq motifs homer
updated by jared.andrews07 jared.andrews07 • written by Ronin • Ronin
0 votes
0 replies
464 views
Unicycler hybrid assembly run is frozen for 2 days with no error message
hybrid-assembly unicycler
written by langziv • langziv
1 vote
0 replies
631 views
Calculating if cell proportions are significant using propeller
regression proportions scrna-seq
written by mropri mropri
0 votes
1 reply
886 views
Overestimate of ATAC-seq differential peaks
macs2 atac-seq deseq2
updated by jared.andrews07 jared.andrews07 • written by Darryl • Darryl
0 votes
2 replies
1.6K views
Mapping Mouse RNAseq Marker Genes to Reactome Pathways
rna-seq single-cell pathways reactome
updated by Menna • Menna • written by el24 • el24
0 votes
3 replies
1.2K views
Finding common genes for evolutionary analyses after finding DEG's from different env. stressed plants
deg galaxy-server rna-seq
written by candy • candy
0 votes
2 replies
1.3K views
I need to index a GTF (gene transfer format) annotation file
gatk funcotator gtf hla-typing
updated by Ram Ram • written by doodle • doodle
0 votes
6 replies
3.6K views
Does the order of the batch variable matter in Limma design matrix?
batch
updated by Gordon Smyth Gordon Smyth • written by CTLong • CTLong
0 votes
2 replies
1.0K views
Genomic Context Analysis
genomic-context-analysis neighboring-genes
updated by Ram Ram • written by Thais • Thais
1 vote
3 replies
1.1K views
Biological replicate for WGS sequencing
ngs biological replicate wgs
updated by GenoMax GenoMax • written by octpus616 • octpus616
14 votes
21 replies
6.9K views
Tool: New Illumina error mode, new BBTools release (39.09) to deal with it
assembly novaseqx illumina poly-g bbtools
updated by GenoMax GenoMax • written by Brian Bushnell Brian Bushnell
1 vote
4 replies
2.2K views
Forum: What companies do you recommend for targeted sequencing?
targeted-sequencing
written by hello_bioinf • hello_bioinf
0 votes
1 reply
828 views
Low Coverage WGS (Human) vs WES Hardware Requirements
macbook-pro wgs
updated by GenoMax GenoMax • written by rodrigo.sanchez • rodrigo.sanchez
0 votes
0 replies
709 views
News: Online course: Population Genomic Inference from Low-Coverage Whole-Genome Sequencing Data
low-coverage-wgs angsd population-genomics
updated by Ram Ram • written by Physalia-courses Physalia-courses
0 votes
1 reply
1.0K views
Intermixed clusters and cell types in UMAP for scRNA-seq analysis
pbmc umap scran-seq
updated by jaro.slamecka jaro.slamecka • written by bio_info • bio_info
0 votes
4 replies
2.5K views
CIBERSORTX disable quantile normalization
rna-seq
written by fifty_fifty • fifty_fifty
0 votes
8 replies
3.3K views
How to download Gene annotations (by Ensembl) track on NCBI Sequence Viewer 3.49.0
sequence-viewer ensembl ncbi
updated by kkun • kkun • written by rahu • rahu
0 votes
0 replies
677 views
Recommended Tool for Somatic SV Discovery in Tumor-Only WES Data
sv wes tumor-only
written by George • George
0 votes
3 replies
1.8K views
Bcftools error: Reference allele does not match the reference
bcftools liftover
updated by Pierre Lindenbaum Pierre Lindenbaum • written by ktg25 • ktg25
0 votes
0 replies
774 views
Fusion analysis with UMI deduplication
umi fusion deduplication star
written by Xira_D • Xira_D
0 votes
2 replies
1.5K views
Limma covariates treatment is changing my significance results
limma differential-expression
written by ijarne • ijarne
0 votes
0 replies
633 views
imputation A1, A2
a1 imputation a2 gwas
written by SeoG • SeoG
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