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0 votes
1 reply
1.6K views
is there any possible way I can tell whether the presence of more bands in the elutions are because of insufficient washing or truncated/degraded protein still having the his tag?
protein purification offtopic
updated by GenoMax 16K • | written by mohammed 0
0 votes
1 reply
829 views
MFE in RNA structure
rna
updated by Mensur Dlakic 3.0K • | written by Ebtihal 0
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1 reply
1.3K views
nf-core/sarek pipeline : adding genome index in config file
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updated by Maxime Garcia 41 • | written by ashaneev07 5
0 votes
1 reply
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N content - Sequence Quality
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updated by GenoMax 16K • | written by j.k3096 2
0 votes
1 reply
1.1K views
Seeking Explanation for the "Secondbest" Method in Gene log2FC Calculations with CRISPR Data in MAGeCK
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updated by ATpoint 9.1K • | written by yanbinwan 0
0 votes
1 reply
1.2K views
What is the proper output for a psiclass_vote.gtf file, and how does one add gene names and strand to gtf files?
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written by DdogBoss 7
0 votes
4 replies
1.8K views
Commands for processing mixed 4-sample scRNA-seq data with cellranger
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written by Apprentice 19
0 votes
0 replies
1.5K views
Forum: Survey: Exploring RNA Data Quality in Genomics
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written by chrisk 0
1 vote
14 replies
10K views
Forum: New Macbook M4 laptops (Pro vs Max)
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updated by GenoMax 16K • | written by tommygoe 1
0 votes
2 replies
1.2K views
miRBase is down
mirbase
written by tyron.chang 0
0 votes
0 replies
966 views
miRNA-seq Analysis: Is a low percentage of assigned reads normal when using a miRBase gff file for counting
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written by Sib 8
0 votes
1 reply
1.6K views
Sample-wise raw count matrix from 10x multiplexed data
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updated by kkohl 0 • | written by AB 39
0 votes
1 reply
990 views
How to Test Statistical Association Between Clusters and Conditions in scRNA-seq Data?
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updated by jared.andrews07 1.9K • | written by fifty_fifty 10
0 votes
1 reply
942 views
GATK is throwing an error when using dbsnp hg19 or GCRCh 38 build 156
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updated by Ram 4.5K • | written by Karibu 0
0 votes
3 replies
3.0K views
Cattle reference genome ARS-UCD2.0
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updated by Michael Dondrup 5.6K • | written by am29 6
0 votes
1 reply
920 views
RNA-seq WGCNA anlysis
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updated by ATpoint 9.1K • | written by SeoG 1
0 votes
0 replies
606 views
Splitting single cell data
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written by otieno43 4
0 votes
0 replies
938 views
News: Online course - Flow cytometry data analysis with R/Bioconductor
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written by Physalia-courses 281
0 votes
0 replies
949 views
Help selecting WGCNA soft thresholding power
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updated by andres.firrincieli 412 • | written by TNF_alpha 0
0 votes
0 replies
1.1K views
Deciding Between TOIL Processed Data (Xena) vs. TCGA Biolinks Data for DE Analysis
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written by lyan125 1
0 votes
1 reply
1.1K views
working with shapeit.
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updated by sentigeo 0 • | written by sanchit000singhdr098 0
0 votes
1 reply
1.1K views
rRNA contamination in RNASeq fdata
contamination rrna rnaseq
updated by dsull 781 • | written by rajdeepboral00 7
0 votes
2 replies
1.1K views
inserting Normalized count data
deseq2
updated by ATpoint 9.1K • | written by Saeed 1
1 vote
5 replies
2.2K views
high IgG background
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updated by ATpoint 9.1K • | written by 17318598206 3
0 votes
2 replies
1.4K views
Unexpected results: Conservation of cCREs
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written by Yacob 0
0 votes
3 replies
2.6K views
FastQC polyA and polyG trimming in RNA-seq
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written by joonhong kwon 7
0 votes
4 replies
1.9K views
Finding Orthologs in Unannotated Genomes
orthologs
written by triplee0305 2
0 votes
4 replies
1.7K views
Differential Expression for a predefined one or multiple genes and multiple testing
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updated by Gordon Smyth 916 • | written by p-radwan.derbala 0
2 votes
0 replies
26K views
Herald: The Biostar Herald for Monday, December 23, 2024
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written by Biostar 380
0 votes
6 replies
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dbSNP and indels
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updated by biology.may20 2 • | written by wonde2000 0
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1 reply
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Retrieve query start and end positions on reverse strand with Pysam
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updated by Mark 173 • | written by María José 1
1 vote
0 replies
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Job: Looking for Internship/Junior Bioinformatics Opportunity
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written by Felix 1
0 votes
0 replies
786 views
Seeking Tool for Transcription Factor Motif Enrichment Analysis on RRBS Dataset
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updated by Ram 4.5K • | written by Srinka 2
0 votes
2 replies
1.4K views
can we perform separate channel analysis of two-channel Agilent microarray data?
two-channel microarray
written by Bini 0
0 votes
0 replies
763 views
Finding Similar Subsequences From Protein Sequences
pfam hmmer protein blast
written by jet 0
0 votes
2 replies
1.5K views
Can different genes encode the same proteins?
uniprot genes proteins enzymes
updated by michael.ante 408 • | written by songyeon0425 0
0 votes
3 replies
1.7K views
Stingtie error: the input alignment file is not sorted
.sam .bam .gtf stringtie
updated by Pierre Lindenbaum 17K • | written by todd.ugine 0
0 votes
2 replies
1.3K views
edgeR customized normalization for differential analysis
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updated by 1769mkc 127 • | written by Wu-Sheng Zhang 1
0 votes
3 replies
1.5K views
scRNA - Illumina naming convention unclear
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updated by GenoMax 16K • | written by kristina 0
0 votes
1 reply
857 views
Help finding error in fasta file
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updated by Ram 4.5K • | written by nreynolds 0
0 votes
8 replies
2.9K views
How to get Normal Samples from TCGA?
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updated by Zhenyu Zhang 130 • | written by Jaber 3
0 votes
3 replies
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Challenges Using KING for Relatedness Analysis with Non-Standard Genomes (8000 Chromosomes)
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written by alex 0
0 votes
0 replies
598 views
Assessment of upstream/downstream variants
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written by Nesma 0
0 votes
1 reply
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STARsolo sorted bam contains 'non-whitelisted' barcodes
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updated by dsull 781 • | written by mk 31
1 vote
0 replies
1.2K views
News: I created an NGS data analysis tutorial site (ngs101.com)!
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updated by GenoMax 16K • | written by ngs101.com 39
2 votes
5 replies
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Wrong SNP calling in my bcftools output
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written by pablo 35
0 votes
0 replies
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How to Convert Ensembl Gene and Transcript IDs to RefSeq mRNA and Gene IDs from isoforms data?
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written by mauricio.1313 0
0 votes
1 reply
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How to annotate all SNPs from a BAM file, not just the variants?
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updated by Mark 173 • | written by Andresa Capodifoglio 4
0 votes
4 replies
1.7K views
Manually modified gtf cannot be loaded into featureCounts STAR
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updated by GenoMax 16K • | written by francesco.limone 0
0 votes
3 replies
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Computational way to distinguish between polyA and total RNA bulk RNA-seq
rna-seq
written by predeus 212
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