hi all, Iam working on RNA structure by RNA fold i checked the wild structure and the mutant one the MFE changed from -16.7 (in wild type) to -20 is this stabilizing mutant or destabilizing? Anyone can explain how we can interpret the change in the MFE
•
written
by
Ebtihal •
1 answer
Lower free energy - more negative - indicates a more stable structure. Either a larger number of bonds, or the same number of stronger bonds, will make a thermodynamically more stable structure. In the case of structured RNA molecules that usually means replacing an A-T pairing with a G-C pairing, or adding base interactions where they didn't exist before. Keep in mind that a more stable RNA molecule does not always mean a functionally better molecule.
By the way, Google easily answers your main question:
https://www.google.com/search?q=is+lower+Minimum+Free+Energy+better
Log in to answer this question.
-
Comparing GSEA mutant data to wild type
written by conmul •
-
Performing Needleman-Wunsch Global Alignment for multiple mutant proteins against wild-type
written by VolEr •
-
Calculating p-value and adjusted p-value for pre-normalized microarray data with fold change precal…
written by Shero •
-
Comparing log2-fold changes to find elements with virtually the same log2-fold changes across diffe…
written by robrob •
-
Help with statistics terms
written by caitlindarling11 •
-
Pathway analysis and Gene enrichment Analysis Queries
written by bioinforesearchquestions
-
Sleuth: Combined lrt and wt tests, clustered heatmap
written by bioinfo8
-
Unexpected Molecular Dynamics results
written by f.dehghanian68 •
-
RNA-seq Anova on three groups
written by BM
-
R Limma Package, Ebayes, Contrast Matrix
written by Cirrus •
