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0 votes
1 reply
1.5K views
codon adaptation index (CAI) estimation
codon-usage
updated by Ram 4.5K • | written by rthapa 9
0 votes
3 replies
1.1K views
High adj. p-value/FDR despite large FC and high expression levels
edger deseq2 rna-seq
updated by yura.grabovska 94 • | written by nik.kraemer 1
0 votes
1 reply
712 views
Why Is My Uniquely Mapped Read Count Higher in Samtools Compared to Bowtie2?
bowtie2
updated by Istvan Albert 10K • | written by daffodil 1
0 votes
0 replies
347 views
XR-seq processing pipeline
xr-seq
written by sleepystudent 0
0 votes
0 replies
498 views
Smart-Seq3 intron+exon meaning
exon r smart-seq3 plots intron
written by Evlampia 0
0 votes
0 replies
551 views
I'm not sure what is going on with my DEXSeq object
genomicranges dexseq help rna-seq summarizedexperiment
written by cftattersfield 0
0 votes
1 reply
850 views
ChIP-seq signal across bed regions
normalisation chipseq quantification signal
updated by mark.ziemann 199 • | written by sk_24 0
0 votes
2 replies
1.1K views
Reading location of a feature of a miRNA entry in miRBase in EMBL format
format mirbase embl
written by Coleman 0
0 votes
3 replies
1.5K views
How can you interpret a phased VCF with no "phase set" field (PS)
variants vcf phasing genotyping
written by cmdcolin 448
0 votes
6 replies
2.2K views
How to make a .gaf file for GO analysis
ngs go
written by noodle 65
0 votes
5 replies
1.6K views
bigWig size difference between variableStep and fixedStep wig files
bigwig wig
written by notthebmovieactor 0
0 votes
6 replies
1.5K views
Unannotated reads through STAR
star igv unannotated-reads
written by rajdeepboral00 7
0 votes
2 replies
862 views
multiple outputfiles after bamtofastq for same library
cellranger 10x bamtofastq
updated by GenoMax 16K • | written by Tolga 3
0 votes
6 replies
1.5K views
Hisat2 index genome
hisat2
written by frarodmar17 4
9 votes
22 replies
66K views
Public Electronic Health Records Database
database
updated by Alex 0 • | written by Abhi 163
0 votes
1 reply
639 views
Comparison of metabolic data between groups (WT vs KO)
metabolism
updated by ATpoint 9.1K • | written by solene.vardon 0
0 votes
6 replies
1.7K views
Estimating mRNA Half-Life from Single Time Point Data Using mRNA/Ribo-seq Expression Data
regression mrna rna-seq ribo-seq
updated by Ram 4.5K • | written by Ramya 0
0 votes
0 replies
431 views
Random effect model RNAseq
rma metafor
written by a.stef.44 1
0 votes
3 replies
1.1K views
Barley, alignment rates
barley alignment-rate
written by IrK 10
0 votes
0 replies
1.0K views
News: Introduction to Python for biologists course
ngs python programming
written by Physalia-courses 281
5 votes
15 replies
45K views
Convert between RefSeq and Ensembl Transcript?
refseq ensembl
updated by GenoMax 16K • | written by pwg46 54
0 votes
5 replies
1.4K views
HELP with PacBio RS reads for epigenetics
pacbio bax.h5 rs
updated by Billy Rowell 52 • | written by sarajorqueraortega 0
0 votes
2 replies
1.1K views
Post-sequencing basecalling error in MinKNOW
minknow dorado long reads nanopore
written by Cedrick 1
0 votes
2 replies
780 views
why knocking down LEMD2 using siRNA gives handful DEGs?
knockdown sirna lemd2
written by goyal.shiwali 2
0 votes
4 replies
2.1K views
'Model matrix not full rank'
deseq2 design matrix
updated by Santosh Anand 582 • | written by Rish 0
0 votes
1 reply
980 views
Change threads (-T) in online Galaxy for featureCounts
featurecounts rna-seq threads galaxy
updated by GenoMax 16K • | written by raeven.bastock 0
0 votes
1 reply
1.1K views
Process Illumina Sequencing Data.
human illumina libraries
updated by GenoMax 16K • | written by María José 1
1 vote
1 reply
832 views
vgconstruct crashes
vg
updated by anovak 18 • | written by Uveyik 8
0 votes
0 replies
1.5K views
Tutorial: ONLINE COURSE – Machine Learning using Python (MLUP01)
machine-learning python
updated by Ram 4.5K • | written by oliverhooker 14
0 votes
2 replies
859 views
Demultiplexing Custom Barcodes
rna-seq custom-barcodes demultiplexing
written by meetmet 1
0 votes
6 replies
2.1K views
Sequence length distribution after trimming
bbduk trimming fastqc fastq
updated by GenoMax 16K • | written by ijarne 3
0 votes
1 reply
873 views
de novo and reference-guided assemblies
de-novo reference-guided assemblies
updated by Ram 4.5K • | written by ZuelTech 0
1 vote
0 replies
26K views
Herald: The Biostar Herald for Monday, January 27, 2025
herald
updated by Istvan Albert 10K • | written by Biostar 380
0 votes
6 replies
1.4K views
Differential Expression Analysis in single-cell data
single-cell
written by frarodmar17 4
0 votes
1 reply
819 views
Accounting for Within-Subject Correlation in Microbiome Multiclass Classification
microbiome classification longitudinal panel repeated-measures
updated by Mensur Dlakic 3.0K • | written by ssko 2
0 votes
7 replies
1.5K views
Genome 2 GEnome Alignment and Unmapped contigs extraction
assemblies minimap2 genome alignment fungus
written by SomeOne 33
0 votes
1 reply
659 views
Merge genotypes of two vcf files
vcf vcftools
updated by Pierre Lindenbaum 17K • | written by pablo 35
0 votes
1 reply
989 views
Abyss output scaffolds.fa file contains kmer sized scaffolds
scaffolds abyss
updated by lieven.sterck 1.6K • | written by Bushra Bibi 8
0 votes
2 replies
1.1K views
Detect changes in cell viability with bulk RNA-seq?
deg viability rna
written by txema.heredia 31
0 votes
2 replies
1.5K views
Origin of unmapped reads
scrna bam mapping sequencing shortreads
updated by dthorbur 350 • | written by Kay 0
0 votes
0 replies
409 views
PNI Cohort for Gastric Gastric
invasion dataset request perineural
written by p-radwan.derbala 0
0 votes
3 replies
1.4K views
biopython UI?
biopython
written by tada 0
0 votes
0 replies
533 views
Using Limma (Batch effect removal bulk rna-seq)
batchremoval bulkrnaseq limma
updated by GenoMax 16K • | written by odi 3
0 votes
1 reply
1.5K views
Covarites in GWAS via GCTA
covariate gcta fastgwa
updated by GenoMax 16K • | written by Star:) 5
0 votes
1 reply
1.8K views
Question about Kraken2 core_nt vs nt database results.
database kraken2 kmer ncbi
updated by GenoMax 16K • | written by Mark 6
0 votes
0 replies
730 views
News: Spring School in Bioinformatics - ONLINE, 31 March–4 April 2025
ngs phylogenomics rnaseq
written by Physalia-courses 281
0 votes
2 replies
1.1K views
Protein annotation, gene annotation, hordeum vulgare RNA-Seq
protein vulgare rna-seq hordeum gene
updated by colindaven 844 • | written by martta95 1
0 votes
1 reply
1.8K views
On applying spike-in-derived scaling factors to ChIP-seq data: adjust for background signal (input) or not?
spike-in deeptools normalization chip-seq scaling-factor
written by kalavattam 85
0 votes
4 replies
1.8K views
WGCNA: Can we use high power for signed network?
powerselection rna-seqdata wgcna networkanalysis
written by Vinesh 1
0 votes
2 replies
1.2K views
SRA fastq modify by 10x valid fastq
fastq
written by wyuan37 0
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