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1 vote
11 replies
12K views
ATAC-seq fragment size distribution - huge spike at 150 bp
atac-seq
updated by yuntan1992 • yuntan1992 • written by Simon • Simon
0 votes
15 replies
12K views
Tools for raw reads that estimate the percentage of reads that hit marker genes using protein BLAST or more advanced approaches based on protein HMM profiles of marker PFAM domains
data raw reads shotgun
written by shevch2009 • shevch2009
0 votes
5 replies
8.3K views
RNAseq requirements: Biological and Technical replicates
rna-seq
updated by jkim • jkim • written by Rim • Rim
0 votes
2 replies
6.2K views
How to connect the UCSC when i get the DMR.plot?
dna methylation dmr
written by Wren • Wren
0 votes
7 replies
11K views
how to start
clarity
updated by patrickdm • patrickdm • written by raychmuthoni • raychmuthoni
0 votes
4 replies
7.9K views
News: Feedback needed on new user-friendly BED file tool
bedtools bed
updated by ATpoint ATpoint • written by nadia • nadia
2 votes
1 reply
5.4K views
BedToIntervalList (Picard) and IntervalListTools (Picard)
variant rna-seq picard calling gatk
updated by Pierre Lindenbaum Pierre Lindenbaum • written by Beyza • Beyza
0 votes
0 replies
5.7K views
News: Workshop:Virtual Genome Assembly, Sept. 16-18, 2025
virtual workshop uconn genomeassembly
written by zsc25001 • zsc25001
0 votes
3 replies
7.9K views
HIV virus - GTF annonation
hiv
updated by Ales • Ales • written by maxdlf12 • maxdlf12
0 votes
0 replies
5.7K views
Job: Tenure Track Professor of Translational Bioinformatics and Precision Medicine
bioinformatics liquid-biopsy computational-biology
written by klaus.wagner • klaus.wagner
1 vote
9 replies
12K views
HIV : gtf or gff annotation
gtf annotation hiv gff
updated by Ales • Ales • written by Nicolas Rosewick Nicolas Rosewick
1 vote
1 reply
6.3K views
DESeq2 time-series - full model matrix is less than full rank
full-rank design deseq2 time-series
updated by swbarnes2 swbarnes2 • written by gogeni5529 • gogeni5529
0 votes
1 reply
5.9K views
Removing PCR duplicates in gam file
giraffe vg
updated by colindaven colindaven • written by gulin201909 • gulin201909
0 votes
0 replies
5.4K views
Job: 2-year postdoc is now available at my lab in Lund, Sweden, in AI and Forensics
postdoc forensics crime ai
written by eranelhaik • eranelhaik
1 vote
3 replies
6.9K views
RNAseq edgeR :considering interaction is needed?
edger interaction rnaseq
updated by SamGG • SamGG • written by tesler • tesler
0 votes
16 replies
14K views
Parabricks : Number of GPUs requested (2) is more than number of GPUs (0) in the system., exiting.
haplotypecaller parabricks gpu
written by Pierre Lindenbaum Pierre Lindenbaum
0 votes
3 replies
9.0K views
CDS prediction for newly assembled transcripts
rna-seq transcriptome assembly cds
updated by Ales • Ales • written by I0110 I0110
0 votes
2 replies
8.2K views
Gene feature information missing in Stringtie merged assembly
rna-seq assembly gtf
updated by Ales • Ales • written by waqaskhokhar999 waqaskhokhar999
0 votes
3 replies
8.0K views
Criteria for choosing variants
variant rnaseq
written by ramiro.barrantes • ramiro.barrantes
1 vote
5 replies
10K views
How to fix "Please indicate the file directory in 'setting' file?" for MaxBin2?
maxbin2 binning metagenomics
updated by GenoMax GenoMax • written by O.rka O.rka
0 votes
4 replies
8.0K views
Tutorial: Perform Hierarchical Clustering in R with ggdendro Visualization
statistics hierarchical_clustering ggdendro r
written by Jeremy • Jeremy
2 votes
3 replies
5.5K views
Difficulty Imputing Missing SNPs for Haplotype Construction in PyPGx
pypgx haplotype imputation beagle genotyping
updated by Aleksandra • Aleksandra • written by Andresa Capodifoglio • Andresa Capodifoglio
2 votes
1 reply
6.1K views
CBioPortal: What does Mutation type: Targeted_Region means?
cbioportal variants cancer oncokb
updated by Aleksandra • Aleksandra • written by Frans • Frans
0 votes
0 replies
4.7K views
News: Machine Learning for Multi-Omics Integration – University of Barcelona, 15–17 December
dataintegration machine-learning mult-omics singlecellrnaseq
written by Physalia-courses Physalia-courses
0 votes
10 replies
9.2K views
Calling star alleles with PGx-POP on my VCF file
vcf alleles pgx-pop star
updated by Andresa Capodifoglio • Andresa Capodifoglio • written by Vanish007 • Vanish007
0 votes
1 reply
4.6K views
Query Regarding NGS Analysis with TruSeq UMI Adapters
twist
updated by GenoMax GenoMax • written by 1769mkc 1769mkc
0 votes
4 replies
5.3K views
Learning Bioinformatics doing Projects
bioinfo projects offtopic
written by Anjali • Anjali
1 vote
15 replies
10K views
High percentage of intronic/intergenic reads in RNA-seq
rna-seq
updated by i.sudbery i.sudbery • written by lluc.cabus • lluc.cabus
0 votes
0 replies
4.5K views
Job: Assistant Professor in Genomics (Colgate University)
genomics assistant-professor
updated by Ram Ram • written by Ahmet • Ahmet
0 votes
6 replies
5.2K views
deeparg-takes-too-many-threads
shotgun deeparg raw reads
updated by Dave Carlson Dave Carlson • written by shevch2009 • shevch2009
0 votes
2 replies
4.8K views
When to merge RNA-seq files with multiple sequencing lanes
rna-seq deseq2 trinity cd-hit goseq
updated by GenoMax GenoMax • written by Olivia • Olivia
0 votes
1 reply
4.1K views
Regress out covariate in boxplot
ggplot2
updated by LChart LChart • written by Nona • Nona
0 votes
1 reply
4.3K views
DESeq2 design: longitudinal two timepoint study with treatment effect
longitudinal treatment deseq2 effect
updated by LChart LChart • written by jonstug • jonstug
4 votes
0 replies
16K views
Herald: The Biostar Herald for Tuesday, September 02, 2025
herald
written by Biostar Biostar
1 vote
6 replies
7.8K views
protein interaction prediction
protein interaction
written by Fatemeh • Fatemeh
0 votes
7 replies
5.7K views
Add reference genomes as an outgroup to a phylogenetic tree
phylogenetic tree
updated by Michael Dondrup Michael Dondrup • written by Álvaro • Álvaro
0 votes
2 replies
13K views
News: FINAL CALL: In-Person Single-Cell RNA-Seq Data Analysis Workshop (October 6-8 in Berlin)
scrnaseq singlecell transcriptomics
written by ecSeq Bioinformatics • ecSeq Bioinformatics
0 votes
0 replies
3.7K views
News: Hands-On NGS Epigenomics Data Analysis Workshop (November 3-5 in Munich, Germany)
bisulfite-seq chromatin chip-seq methylation atac-seq
written by ecSeq Bioinformatics • ecSeq Bioinformatics
1 vote
0 replies
3.9K views
Job: Looking for mentorship and collaboration opportunities in RNA-seq machine learning (R-based)
r bayesian machine-learning rna-seq
updated by GenoMax GenoMax • written by AcheampongAndy • AcheampongAndy
0 votes
6 replies
5.2K views
Low percentage of classified reads (~18%) after Kraken2 analysis – is this expected?
kraken2 metagenomics shotgun
written by sumah.1si21bt017 • sumah.1si21bt017
0 votes
0 replies
3.7K views
News: online course Sex Chromosome Evolution (6–10 October)
genomics evolution sexchromosome
written by Physalia-courses Physalia-courses
3 votes
4 replies
3.8K views
Are these 10x samples too low quality for downstream analysis?
single-cell scrnaseq seurat 10x
updated by songli • songli • written by AHerik • AHerik
0 votes
5 replies
5.2K views
Split chromosome into smaller fragments to save memory when build xg and gcsa index.
vg
updated by Jordan M Eizenga • Jordan M Eizenga • written by 李经纬 • 李经纬
2 votes
2 replies
3.8K views
Universal Human Reference RNA Sample
rna
updated by michael.j.apostolides • michael.j.apostolides • written by kstangline • kstangline
0 votes
2 replies
3.0K views
Question about substitution of KEGG
annotation kegg functional gene
written by dxh0222 • dxh0222
0 votes
1 reply
3.0K views
Length of 'dimnames' [1] not equal to array extent
singlecellexperiment
written by fra.r.silvestro • fra.r.silvestro
0 votes
1 reply
2.8K views
Deeptools PlotProfile
computematrix deeptool heatmap chip-seq bamcoverage
updated by ATpoint ATpoint • written by Irene • Irene
0 votes
0 replies
2.7K views
News: online course: Species occurrence data: download and cleaning with R
data-cleaning biodiversity species-ocurrence
written by Transmitting Science communication • Transmitting Science communication
0 votes
3 replies
3.1K views
Local Synteny Analysis with Uncharacterized Genes
synteny
updated by Joe Joe • written by snaledale • snaledale
0 votes
3 replies
3.3K views
Help with metagenomics viral
sequencing genomic metagenomic virology virus
updated by dr_cfoster dr_cfoster • written by CECEL • CECEL
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