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0 votes
4 replies
1.4K views
Discrepancy in Q-score assessment of ONT reads in Nanopore and third-party software repors
seqkit q-score fastqc 16s-wf nanoplot
written by k-tarasov 1
0 votes
4 replies
1.5K views
How to create a consensus of a contig with samtools or bbmap?
contig bbmap consensus samtools genome
updated by jkbonfield 133 • | written by marongiu.luigi 79
0 votes
3 replies
1.2K views
Validating snRNA-seq cell type by correlating with other datasets
rna-seq scrna-seq snrna-seq
updated by ATpoint 9.1K • | written by Ben 0
0 votes
2 replies
989 views
What is the length of the longest ORF appearing in reading frame 2 of any of the sequences?
python
written by fra.r.silvestro 1
0 votes
1 reply
802 views
Variant Normalization
vcf
updated by Istvan Albert 10K • | written by spesks 0
0 votes
2 replies
1.6K views
Should differential expression analysis be incorporated in cross validation for training machine learning models?
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written by yordany.perdigon 0
0 votes
0 replies
602 views
News: Autumn School in Bioinformatics – Online, 20–24 October
ngs phylogenomics rnaseq singularity docker
updated by Pierre Lindenbaum 17K • | written by Physalia-courses 281
0 votes
1 reply
936 views
Issue with Snippy 4.6.0
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updated by GenoMax 16K • | written by bioinfo_enthusiast 0
0 votes
1 reply
907 views
1 vs 1 DEG analysis in scrna seq data
deg
updated by ATpoint 9.1K • | written by carolofharvest 6
2 votes
0 replies
694 views
Tool: ORFanage: by-reference protein annotation and comparison for transcriptome assembly
orf rna-seq assembly annotation transcriptome
written by Ales 10
0 votes
2 replies
2.0K views
scVI vs Harmony, which is better for cell type based clustering in brain tissue?
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updated by ATpoint 9.1K • | written by biotrekker 11
1 vote
0 replies
647 views
News: Manual Genome Curation using PretextView course
genome-assembly pretextview hi-c manuel-curation
written by Physalia-courses 281
0 votes
11 replies
3.3K views
DESeq2 on metagenome KO counts
abundance kegg ko deseq metagenome gene
updated by Aleksandra 19 • | written by young_bioinformatician 25
0 votes
0 replies
697 views
News: Introduction to Processing and Analysis of Spatial Multiplexed Proteomics Data
spatial proteomics omics
updated by GenoMax 16K • | written by oliverhooker 14
0 votes
1 reply
971 views
FACS quality control based on size and doublet detection in scRNA-seq
single-cell rna-detection facs doublet scrna-seq
updated by Ram 4.5K • | written by carolofharvest 6
0 votes
2 replies
1.2K views
combine VCF from diploid reference/haplotypes for the same sample
bcftools combine vcf
updated by Kevin Blighe 9.0K • | written by Matteo Ungaro 16
1 vote
2 replies
1.1K views
Recommendations for 200 SNP markers genotyping
sequencing dna genotyping marker
updated by Aleksandra 19 • | written by PolenP 1
0 votes
3 replies
1.3K views
Interpreting genomic features distribution for CUT&RUN peaks
cut_and_run genomic features
updated by rfran010 174 • | written by Rozita 4
1 vote
6 replies
2.6K views
Difficulty running FoldX in linux
ddg foldx
updated by strayeroliver 0 • | written by strayeroliver 1
0 votes
4 replies
1.8K views
Active site using castpfold
activesite castpfold docking
written by Ria 0
0 votes
12 replies
2.9K views
Differences between published differential gene expression results and own analysis on RNA-seq data
r tcga limma
updated by Zhenyu Zhang 130 • | written by vernonlim98 0
0 votes
6 replies
1.5K views
downloading raw bam files from pacbio sequencer
raw bam_file raw_subreads reads
updated by GenoMax 16K • | written by pranavdatar01 1
0 votes
2 replies
1.6K views
Transverse data
excel unix
updated by basu 0 • | written by Sidra 0
0 votes
3 replies
1.0K views
Pseudogene - scarce info
pseudogene mastersdegree
updated by ATpoint 9.1K • | written by mf810 1
0 votes
4 replies
2.5K views
HIV NL4-3 transcriptome fasta
rna-seq clip-seq
updated by Ales 10 • | written by xiaoleiusc 14
0 votes
1 reply
709 views
Doubt on docking
docking active-site
updated by Ram 4.5K • | written by Ria 0
1 vote
0 replies
624 views
News: Online course: RNA sequencing data analysis using R and Bioconductor - 3–14 November
r differentialexpressionanalysis rnaseq bioconductor
written by Physalia-courses 281
0 votes
3 replies
1.0K views
Nextflow MultiQC runs multiple times due to FASTQC zip name collisions
fastqc nextflow multiqc
updated by Phil Ewels 160 • | written by DdogBoss 7
0 votes
2 replies
6.2K views
Best set of tools / workflows for predicting the biosynthesis pathways of complex organic molecules
ngs pathways
updated by Ram 4.5K • | written by Mark 6
0 votes
15 replies
6.0K views
Tools to simulate Illumina short read sequences and ONT long reads with a reference genome
simulate short-read
updated by Ram 4.5K • | written by PolenP 1
0 votes
1 reply
838 views
Corresponding BAM files
bam sra geo fastq
updated by GenoMax 16K • | written by aj123 14
0 votes
2 replies
2.2K views
Analyzing CRISPR and RNAi genome wide screens
crispr rnai meta analysis
updated by Meisam 25 • | written by spacemorrissey 28
0 votes
3 replies
3.3K views
meta data analysis for clustering
meta-analysis clustering functional genomics
updated by Meisam 25 • | written by jiwpark00 24
1 vote
9 replies
4.2K views
GTF file for HIV strain pNL4-3
hiv annotation mapping
updated by Ales 10 • | written by caggtaagtat 194
0 votes
0 replies
685 views
Job: Postdoctoral Fellowship at the American Museum of Natural History's Richard Gilder Graduate School
postdoctoral fellowship
written by Info-rggs 2
0 votes
0 replies
632 views
News: Autumn School in Bioinformatics - online, 20–24 October
ngs phylogenomics rnaseq singularity docker
written by Physalia-courses 281
0 votes
4 replies
1.8K views
Dorado in PowerShell on Windows
windows on powershell in dorado
updated by jared.andrews07 1.9K • | written by sbissi102 1
1 vote
4 replies
1.6K views
Which aligner is more suitable for ONT R10.4.1 Dorado-corrected reads: minimap2 or winnowmap?
winnowmap minimap2 r10.4.1 nanopore
written by tungsega 1
0 votes
2 replies
940 views
Nextflow: How to format input tuple for STAR_ALIGN process with STAR index directory
nextflow
written by DdogBoss 7
0 votes
4 replies
1.2K views
how to respond that my research does not have experimental validation
rebuttal
updated by Mensur Dlakic 3.0K • | written by nuorain 4
0 votes
7 replies
2.2K views
Quantification using salmon in alignment-based mode after minimap2 run
nanopore ont salmon
written by Assa Yeroslaviz 197
0 votes
1 reply
1.2K views
infercnv error at STEP 18 - file descriptor is too large for select()
r 10x singlecell infercnv
written by yura.grabovska 94
0 votes
0 replies
521 views
News: online course - Environmental Metagenomics
illumina metagenomics nanopore mags
written by Physalia-courses 281
0 votes
4 replies
1.3K views
How to convert gff to Sequin format with gff3-to-tbl?
genome annotation conversion sequin gff
updated by GenoMax 16K • | written by marongiu.luigi 79
0 votes
10 replies
3.8K views
Collect COV file by BedCoverage
copynumbervariation clincnv ngs
updated by German.M.Demidov 299 • | written by Ishak 2
0 votes
1 reply
619 views
gnomad par region variants
region variant par gnomad
updated by benformatics 426 • | written by a615ebfb 8
0 votes
2 replies
872 views
Forum: Help with Ideathon
pgs prs brainstorming genomics bioinformatics
written by V K 0
0 votes
2 replies
861 views
Gene expression equivalent of polygenic risk score ?
expression gene
written by Picasa 71
0 votes
1 reply
853 views
Help interpreting BLASTn results and phylogenetic tree for genetics practical
student
updated by GenoMax 16K • | written by simonlydialm 0
0 votes
0 replies
558 views
News: Introduction to CRISPR for Ecology and Evolution Studies (13–16 Oct, online)
genomeediting cas9 crispr evolution
written by Physalia-courses 281
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