FACS quality control based on size and doublet detection in scRNA-seq
If, in our 10x Genomics scRNA-seq data, large cells have already been excluded previously using FACS, does that mean using a doublet detection method in single-cell analysis is unnecessary?
• 966 views
•
link
1 answer
No, it's unrelated. Doublets in 10x happen if two cells get enclosed into the same GEM droplet. This has nothing to do with how flow cytometry determines doublets.
• 0 views
•
link
Log in to answer this question.