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0 votes
2 replies
822 views
deep learning architectures
deep-learning
updated by Ram Ram • written by Deepanshu • Deepanshu
0 votes
1 reply
649 views
Creating a personalized genome assembly for read simulation from illumina WGS data
illumina consensus wgs phasing simulation
updated by colindaven colindaven • written by scsc185 • scsc185
0 votes
5 replies
1.4K views
Prokaryotic RNA-seq: how to handle ncRNAs in featureCounts and DE analysis?
ncrna lcrna trna tmrna
written by MaxMin • MaxMin
0 votes
1 reply
983 views
Unable to download bacterial protein sequences from NCBI using datasets CLI (DOMAIN is not a valid V2reportsRankType error)
prokaryotes datasets ncbi
updated by GenoMax GenoMax • written by Rohan • Rohan
0 votes
0 replies
440 views
News: Introduction to Nextflow online course
reproducibility nextflow training workflow-manager
written by Physalia-courses Physalia-courses
2 votes
1 reply
709 views
Keep FastQC homopolymers or switch to fastp auto-detection?
adapter
updated by GenoMax GenoMax • written by Abieskawa • Abieskawa
0 votes
3 replies
894 views
Is there a term called 'pseudoenrichment' in Cut&Run analysis?
cutandrun chipseq
updated by LChart LChart • written by sribas.chowdhury888 • sribas.chowdhury888
0 votes
2 replies
782 views
Include plasmid sequences in RNA-seq differential expression analysis for bacteria?
rna-seq rnaseq bacteria de plasmid
updated by GenoMax GenoMax • written by MaxMin • MaxMin
0 votes
0 replies
652 views
Forum: For those with in autoimmune research, what are the biggest challenges seen in translating findings into real therapeutics?
drug-discovery autoimmune-disease immunology translational-research
written by greenandblackhack • greenandblackhack
0 votes
2 replies
1.6K views
Nextflow error: “Invalid method invocation call with arguments (java.util.ArrayList)” when connecting processes via joined channels
homer chipseq nextflow
updated by ATpoint ATpoint • written by DdogBoss • DdogBoss
0 votes
0 replies
508 views
Blog: Introduction
introduction
updated by Ram Ram • written by Samuel • Samuel
0 votes
2 replies
1.2K views
Assembling a chloroplast genome using only PacBio data (Illumina reads lost)
chloroplast genome-assembly
updated by shelkmike shelkmike • written by Serena • Serena
0 votes
4 replies
1.7K views
replicate SyRi/plotsr output plot in R
r syri synteny
written by Matteo Ungaro • Matteo Ungaro
0 votes
5 replies
1.6K views
Forum: Idea: Cloud-based genome annotation & visualization tool — looking for feedback
genome-annotation software-development visualization
written by GeneScope • GeneScope
0 votes
4 replies
1.5K views
Access to bulk FAST5 datasets
ont sampling nanopore adaptive
written by matt_arnold_bio • matt_arnold_bio
0 votes
6 replies
1.7K views
SNP annotation tool options (alternatives to ANNOVAR)
annotation annovar
updated by Nicole • Nicole • written by shpak.max • shpak.max
0 votes
3 replies
1.3K views
Differential gene expression analysis between species using methods involving read alignment to transcriptome
rna-seq kallisto tximp degs
written by arsala521 • arsala521
1 vote
3 replies
1.3K views
Low busco scores after annotating genome using same model as another species in genus
braker genome annotation braker3
written by Wilber0x • Wilber0x
0 votes
2 replies
945 views
GurobiError in COMPASS: "Element 47 of a double array is Nan or Inf" despite clean input data
compass scrnaseq metabolism gurobi
written by Binghong • Binghong
0 votes
6 replies
1.6K views
Read group addition order in RNA-seq short variant discovery (SNPs + Indels)
rnaseq variantcalling addreadgroups gatk
updated by GenoMax GenoMax • written by iamsmor • iamsmor
1 vote
0 replies
809 views
Job: Applications Now Open – Summer 2026 Teaching Opportunities [SSPI]
temporary stem summer jobs paid
written by Emaly • Emaly
0 votes
5 replies
4.2K views
FastQC contaminant line error
fastqc contaminant error
updated by Kevin Blighe Kevin Blighe • written by jmah • jmah
0 votes
0 replies
748 views
News: Genomic Data Visualisation with R (online course)
r genomics datavisualisation
written by Physalia-courses Physalia-courses
0 votes
5 replies
3.0K views
Issue with Downloading Genomes from BVBRC
bvbrc genomes
updated by GenoMax GenoMax • written by ryanmaroun3 • ryanmaroun3
0 votes
0 replies
588 views
News: Learn how to make Single Cell Data Analysis easy- Upcoming Webinars
spatialtranscriptomics singlecell dataanalysis pathwayanalysis
written by Nicole • Nicole
0 votes
3 replies
1.0K views
Gene or Exon Counts for DESeq2 in rRNA-Depleted RNA-seq?
rrna depletion gene exon
updated by GenoMax GenoMax • written by n@4j • n@4j
1 vote
7 replies
2.2K views
Falsely high busco score after genome annotation with braker
braker genomeannotation genome annotation braker3
updated by GenoMax GenoMax • written by Wilber0x • Wilber0x
2 votes
0 replies
1.1K views
Forum: Recursive reasoning for variant pathogenicity: early results show promise, need domain expertise to push further
variant-calling deep-learning clinvar pathogenicity-prediction machine-learning
written by Pranava • Pranava
0 votes
5 replies
1.7K views
Clustering with other data type than scRNA-seq using Seurat
countmatrix scrna-seq methylation seurat
updated by Mensur Dlakic Mensur Dlakic • written by npont • npont
0 votes
1 reply
870 views
Is it reasonable to keep only protein-coding genes in highly variable features in scRNA-seq data?
scrna-seq
updated by ATpoint ATpoint • written by carolofharvest • carolofharvest
0 votes
2 replies
1.2K views
Some doubts about GWAS data and MR
mr gwas
updated by Corentin Corentin • written by kanttt16 • kanttt16
0 votes
1 reply
860 views
Bedmethyl to vcf
vcf bedmethyl
updated by oneillkza oneillkza • written by Romualdo • Romualdo
4 votes
0 replies
1.1K views
Herald: The Biostar Herald for Thursday, October 23, 2025
herald
written by Biostar Biostar
0 votes
1 reply
971 views
Repetitive regions annotation
repeatmodeler repeatmasker
updated by samuel.a.odonnell samuel.a.odonnell • written by frarodmar17 • frarodmar17
0 votes
4 replies
1.7K views
Choosing enrichment analysis tool
david metascape enrichr
written by Marlene • Marlene
2 votes
10 replies
2.6K views
Making more complex design in limma removes significant genes
limma
updated by Gordon Smyth Gordon Smyth • written by marek.gierlinski • marek.gierlinski
0 votes
0 replies
864 views
News: Genome Assembly Using PacBio and Hi-C course - online, 10–14 November
pacbio hifi longreads genomeassembly hic
written by Physalia-courses Physalia-courses
0 votes
4 replies
1.5K views
scRNAseq analysis with a few samples (n=2 per group)
deg analysis scrna-seq samples
updated by ATpoint ATpoint • written by Song • Song
0 votes
4 replies
1.3K views
Redundant Genes in scRNA-seq Subclusters
immunology single cell
written by s • s
0 votes
4 replies
1.4K views
gfortran for phyloseq in R
ade4 rcpparmadillo gfortran phyloseq
updated by colindaven colindaven • written by mewgia • mewgia
0 votes
0 replies
728 views
Tool: Xpress-Bio: a lean, real-time Bioinformatics runtime with IGV.js & BLAST integration
web-socket visualization sequence genomics server-sent
written by Ibrahim Tanyalcin Ibrahim Tanyalcin
0 votes
16 replies
6.9K views
bowtie (1) 0% alignment on paired-end RNAseq data
bowtie rna-seq alignment mirna
updated by ATpoint ATpoint • written by gulamaltab • gulamaltab
0 votes
3 replies
2.2K views
Constructing a bread wheat pangenome graph using PGGB: per-chromosome vs. whole-genome approach and integration of assemblies from different sequencing technologies ?
pangenome structural-variation bread-wheat pggb
updated by samuel.a.odonnell samuel.a.odonnell • written by Sony Nguyen • Sony Nguyen
0 votes
0 replies
719 views
News: UConn Virtual Bioinformatics Workshops
training atac-seq workshop chip-seq genomics
written by bioadam • bioadam
0 votes
0 replies
646 views
News: Bioinformatic Analysis of Transposable Elements - online course
transcriptomics genomeassembly manualcuration transposableelements
written by Physalia-courses Physalia-courses
0 votes
7 replies
1.8K views
MOuse Testis Enhancer region
enhancer
updated by i.sudbery i.sudbery • written by Bioinformatics_16 • Bioinformatics_16
0 votes
7 replies
2.2K views
reference-guided assembly tools for short read data?
genomics assembly
updated by Ram Ram • written by Hani • Hani
0 votes
7 replies
1.5K views
Forum: A dentist trying to shift his career
how-to dentistry bioinformatics data science
updated by ATpoint ATpoint • written by Abdelrahman • Abdelrahman
0 votes
4 replies
1.3K views
Limma t and F-tests: should the degrees of freedom correspond to all samples if only some treatments are used in contrast?
limma proteomics
updated by Gordon Smyth Gordon Smyth • written by Sarah • Sarah
0 votes
6 replies
1.9K views
missing values for batch in RNA-seq data
rna-seq limma deseq batch-effect batch
written by donan • donan
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