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0 votes
1 reply
886 views
Ensembl API - Global Ortholog Alignments
alignment api ensembl orthologs
updated by GenoMax GenoMax • written by Nikit • Nikit
1 vote
4 replies
1.7K views
Diffbind or DESEQ2 Chipseq data
deseq2 diffbind chip-seq
updated by james.hawley • james.hawley • written by Irene • Irene
0 votes
2 replies
5.9K views
News: ::FINAL Call:: Only 3 seats left - NGS Epigenomics Data Analysis (November 3-5 in Munich) - BS-Seq, ChIP-Seq, ATAC-Seq, ...
chip-seq atac-seq bs-seq
written by ecSeq Bioinformatics • ecSeq Bioinformatics
1 vote
4 replies
2.1K views
Forum: layoffs in biotech
layoff biotech
updated by dr_cfoster dr_cfoster • written by Dara • Dara
1 vote
1 reply
1.7K views
Drug-Drug similarity database
drug drug_similarity ddi
updated by ardigen • ardigen • written by carlos_marchi carlos_marchi
0 votes
0 replies
675 views
News: Nanopore Direct RNA Sequencing (RNA004)
transcriptomics nanopore rnaseq
written by Physalia-courses Physalia-courses
0 votes
2 replies
1.2K views
Reference VCF with "chr" contigs instead of "number"
vcf reference-genome
updated by Ram Ram • written by GPR GPR
0 votes
1 reply
985 views
DE analysis in nascent RNA Seq based on intron sequences
normalization deseq2 rna-seq nascent
updated by dsull dsull • written by MolGeek • MolGeek
0 votes
4 replies
1.8K views
SNP calling ONT sequenced files
ont snps
updated by Момчил • Момчил • written by blur blur
0 votes
0 replies
732 views
News: RAD-seq Data Analysis - online course
phylogenomics radseq genomics population stacks
written by Physalia-courses Physalia-courses
0 votes
8 replies
3.2K views
Is there a way to produce/convert nhmmer output to a bed file?
bed nhmmer
updated by colindaven colindaven • written by jamie.pike jamie.pike
0 votes
1 reply
644 views
Unique insertion sites Calculation from Himar1 C9 based TnSeq
kossivi
updated by GenoMax GenoMax • written by Kossivi • Kossivi
0 votes
5 replies
1.4K views
BWA-MEM with an array of files
alignment array bwa-mem
updated by LChart LChart • written by garcesj • garcesj
0 votes
0 replies
752 views
Job: Sr. Manager, Clinical Data Science at InterVenn Biosciences
statistics glycoproteomics proteomics machine-learning omics
written by Daniel • Daniel
0 votes
1 reply
774 views
Modeling/simulations using SNPs data
snp
updated by Dave Carlson Dave Carlson • written by Gonzalo • Gonzalo
0 votes
1 reply
876 views
Strugling on the 3'rule of HGVS
notation hgvs variants
updated by Jeremy Leipzig Jeremy Leipzig • written by lacb • lacb
1 vote
17 replies
5.8K views
Extract fastq sequences based on date/time (which is in the header)
sequence fastq
updated by zhanxw • zhanxw • written by a.b.g • a.b.g
1 vote
5 replies
3.7K views
Filter nanopore fastq files by start time
nanopore fastq filter start time
updated by zhanxw • zhanxw • written by sendhelp • sendhelp
0 votes
4 replies
1.3K views
Discrepancy in Q-score assessment of ONT reads in Nanopore and third-party software repors
seqkit q-score fastqc 16s-wf nanoplot
written by k-tarasov • k-tarasov
0 votes
4 replies
1.5K views
How to create a consensus of a contig with samtools or bbmap?
contig bbmap consensus samtools genome
updated by jkbonfield jkbonfield • written by marongiu.luigi marongiu.luigi
0 votes
3 replies
1.1K views
Validating snRNA-seq cell type by correlating with other datasets
rna-seq scrna-seq snrna-seq
updated by ATpoint ATpoint • written by Ben • Ben
0 votes
2 replies
974 views
What is the length of the longest ORF appearing in reading frame 2 of any of the sequences?
python
written by fra.r.silvestro • fra.r.silvestro
0 votes
1 reply
782 views
Variant Normalization
vcf
updated by Istvan Albert Istvan Albert • written by spesks • spesks
0 votes
2 replies
1.6K views
Should differential expression analysis be incorporated in cross validation for training machine learning models?
rna-seq dea tcga learning machine
written by yordany.perdigon • yordany.perdigon
0 votes
0 replies
591 views
News: Autumn School in Bioinformatics – Online, 20–24 October
ngs phylogenomics rnaseq singularity docker
updated by Pierre Lindenbaum Pierre Lindenbaum • written by Physalia-courses Physalia-courses
0 votes
1 reply
889 views
Issue with Snippy 4.6.0
snippy core.vcf snp
updated by GenoMax GenoMax • written by bioinfo_enthusiast • bioinfo_enthusiast
0 votes
1 reply
879 views
1 vs 1 DEG analysis in scrna seq data
deg
updated by ATpoint ATpoint • written by carolofharvest • carolofharvest
2 votes
0 replies
683 views
Tool: ORFanage: by-reference protein annotation and comparison for transcriptome assembly
orf rna-seq assembly annotation transcriptome
written by Ales • Ales
0 votes
2 replies
2.0K views
scVI vs Harmony, which is better for cell type based clustering in brain tissue?
harmony scvi single cell
updated by ATpoint ATpoint • written by biotrekker • biotrekker
1 vote
0 replies
638 views
News: Manual Genome Curation using PretextView course
genome-assembly pretextview hi-c manuel-curation
written by Physalia-courses Physalia-courses
0 votes
11 replies
3.2K views
DESeq2 on metagenome KO counts
abundance kegg ko deseq metagenome gene
updated by Aleksandra • Aleksandra • written by young_bioinformatician young_bioinformatician
0 votes
0 replies
670 views
News: Introduction to Processing and Analysis of Spatial Multiplexed Proteomics Data
spatial proteomics omics
updated by GenoMax GenoMax • written by oliverhooker oliverhooker
0 votes
1 reply
969 views
FACS quality control based on size and doublet detection in scRNA-seq
single-cell rna-detection facs doublet scrna-seq
updated by Ram Ram • written by carolofharvest • carolofharvest
0 votes
2 replies
1.2K views
combine VCF from diploid reference/haplotypes for the same sample
bcftools combine vcf
updated by Kevin Blighe Kevin Blighe • written by Matteo Ungaro • Matteo Ungaro
1 vote
2 replies
1.0K views
Recommendations for 200 SNP markers genotyping
sequencing dna genotyping marker
updated by Aleksandra • Aleksandra • written by PolenP • PolenP
0 votes
3 replies
1.3K views
Interpreting genomic features distribution for CUT&RUN peaks
cut_and_run genomic features
updated by rfran010 • rfran010 • written by Rozita • Rozita
1 vote
6 replies
2.5K views
Difficulty running FoldX in linux
ddg foldx
updated by strayeroliver • strayeroliver • written by strayeroliver • strayeroliver
0 votes
4 replies
1.8K views
Active site using castpfold
activesite castpfold docking
written by Ria • Ria
0 votes
12 replies
2.8K views
Differences between published differential gene expression results and own analysis on RNA-seq data
r tcga limma
updated by Zhenyu Zhang Zhenyu Zhang • written by vernonlim98 • vernonlim98
0 votes
6 replies
1.4K views
downloading raw bam files from pacbio sequencer
raw bam_file raw_subreads reads
updated by GenoMax GenoMax • written by pranavdatar01 • pranavdatar01
0 votes
2 replies
1.6K views
Transverse data
excel unix
updated by basu • basu • written by Sidra • Sidra
0 votes
3 replies
993 views
Pseudogene - scarce info
pseudogene mastersdegree
updated by ATpoint ATpoint • written by mf810 • mf810
0 votes
4 replies
2.5K views
HIV NL4-3 transcriptome fasta
rna-seq clip-seq
updated by Ales • Ales • written by xiaoleiusc xiaoleiusc
0 votes
1 reply
700 views
Doubt on docking
docking active-site
updated by Ram Ram • written by Ria • Ria
1 vote
0 replies
615 views
News: Online course: RNA sequencing data analysis using R and Bioconductor - 3–14 November
r differentialexpressionanalysis rnaseq bioconductor
written by Physalia-courses Physalia-courses
0 votes
3 replies
1.0K views
Nextflow MultiQC runs multiple times due to FASTQC zip name collisions
fastqc nextflow multiqc
updated by Phil Ewels Phil Ewels • written by DdogBoss • DdogBoss
0 votes
2 replies
6.1K views
Best set of tools / workflows for predicting the biosynthesis pathways of complex organic molecules
ngs pathways
updated by Ram Ram • written by Mark • Mark
0 votes
15 replies
5.9K views
Tools to simulate Illumina short read sequences and ONT long reads with a reference genome
simulate short-read
updated by Ram Ram • written by PolenP • PolenP
0 votes
1 reply
825 views
Corresponding BAM files
bam sra geo fastq
updated by GenoMax GenoMax • written by aj123 aj123
0 votes
2 replies
2.2K views
Analyzing CRISPR and RNAi genome wide screens
crispr rnai meta analysis
updated by Meisam • Meisam • written by spacemorrissey spacemorrissey
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