Latest
Open
Jobs
Tutorials
Tags
About
FAQ
Community
Planet
New Post
Log In
New Post
Latest
Open
News
Jobs
Tutorials
Forum
Tags
Planet
Users
Log In
Sign Up
About
Limit : all time
all time
today
this week
this month
this year
117,059 results • Page
4 of 2342
Sort: Votes
Rank
Views
Votes
Replies
95
votes
6
replies
37k
views
In Sam Format, Clarify The Meaning Of The "0" Flag.
sam
updated 13.1 years ago by
Jts
★ 1.4k • written 13.1 years ago by
tflutre
▴ 580
95
votes
5
replies
55k
views
Difference between Hard Clip and Soft Clip in Samtools CIGAR string
samtools
clipped-reads
soft-clip
bam
hard-clip
updated 2.4 years ago by
Ram
44k • written 9.8 years ago by
QVINTVS_FABIVS_MAXIMVS
★ 2.5k
94
votes
31
replies
39k
views
17 follow
What Is The Quickest Algorithm For Range Overlap?
python
alignment
next-gen
sequencing
database
updated 2.1 years ago by
Ram
44k • written 13.7 years ago by
User 1586
▴ 280
94
votes
50
replies
53k
views
16 follow
PCA plot from read count matrix from RNA-Seq
RNA-Seq
pca
updated 2.5 years ago by
BioNovice247
▴ 20 • written 6.5 years ago by
rachel.kubik12
▴ 220
94
votes
16
replies
23k
views
15 follow
Are we tricking ourselves with batch effect correction?
combat
batch-effect
sva
updated 15 months ago by
madbadradscientist
▴ 20 • written 9.0 years ago by
Christian
★ 3.0k
93
votes
23
replies
93k
views
15 follow
Tutorial:
How to Install SamTools, HTSLib, and BCFTools on Ubuntu 18.04
bcftools
samtools
htslib
updated 4 months ago by
clara.apicella
• 0 • written 5.8 years ago by
otwtgin2010
▴ 610
93
votes
19
replies
60k
views
18 follow
What Is A Good Phylogenetic Tree Display Program For Large Data Sets?
phylogenetics-tree
updated 11 months ago by
Ram
44k • written 13.7 years ago by
Niek De Klein
★ 2.6k
93
votes
32
replies
33k
views
26 follow
Linux Distros Best Suited For Bioinformatics?
linux
written 12.3 years ago by
Sat3Lite
▴ 150
93
votes
50
replies
16k
views
17 follow
Forum:
Fda Sends A Warning Letter To 23Andme - Personal Genomics Service Marketing To Be Discontinued
personal-genomics
23andMe
updated 14 months ago by
Ram
44k • written 10.5 years ago by
Istvan Albert
100k
93
votes
18
replies
71k
views
12 follow
Sequence length from Fasta
fasta
updated 17 months ago by
Ram
44k • written 9.5 years ago by
bongbang
▴ 80
92
votes
20
replies
28k
views
13 follow
Is There Any R Or R / Bioconductor Package That Can Make Circular Plots Like Perl / Circos
r
visualization
updated 2.0 years ago by
Ram
44k • written 12.2 years ago by
John
★ 1.5k
92
votes
46
replies
12k
views
28 follow
Best Language For Introductory Programming Course From Within An Introduction Course On Bioinformatics.
programming
subjective
updated 15 months ago by
Ram
44k • written 12.9 years ago by
Andra Waagmeester
3.2k
91
votes
20
replies
20k
views
15 follow
Forum:
Publications for individualized medicine in cancer by whole genome, exome or transcriptome sequencing
N-of-one
WGS
RNA-Seq
next-gen
Exome
updated 19 months ago by
Ram
44k • written 10.0 years ago by
Obi Griffith
20k
91
votes
21
replies
54k
views
13 follow
Should We Remove Duplicated Reads In Rna-Seq ?
rna-seq
read
updated 10.4 years ago by
earonesty
▴ 250 • written 11.6 years ago by
siddharth.sethi5
▴ 270
91
votes
10
replies
108k
views
11 follow
Best Way To Do Pathway Analysis Of A Set Of Genes?
protein
genes
pathway
analysis
function
updated 12.1 years ago by
Guangchuang Yu
★ 2.6k • written 12.1 years ago by
Wayne
★ 1.0k
91
votes
25
replies
62k
views
18 follow
Best Practices/Softwares To Calculate Ka/Ks Ratio
kaks
selection
software
updated 20 months ago by
Michael
54k • written 13.2 years ago by
Jarretinha
3.4k
90
votes
26
replies
39k
views
22 follow
Extracting Sequence From A 3Gb Fasta File
sequence
retrieval
fasta
perl
updated 6.3 years ago by
andplaia
• 0 • written 14.0 years ago by
Divya
▴ 260
90
votes
19
replies
32k
views
12 follow
Tutorial:
Machine Learning For Cancer Classification - Part 1 - Preparing The Data Sets
microarray
bioconductor
expression
classification
updated 14 months ago by
Ram
44k • written 10.6 years ago by
Obi Griffith
20k
90
votes
48
replies
34k
views
29 follow
Tutorial:
Extract Total Non-Overlapping Exon Length Per Gene With Bioconductor
fpkm
rna-seq
bioconductor
updated 6 months ago by
Ram
44k • written 10.6 years ago by
Irsan
★ 7.8k
90
votes
44
replies
60k
views
28 follow
Tool to generate proportional Venn Diagrams?
visualization
updated 2.1 years ago by
Ram
44k • written 13.1 years ago by
Ryan D
★ 3.4k
89
votes
12
replies
21k
views
9 follow
What Do Different Bioinformatics Positions Mean?
career
general
updated 6 months ago by
Ram
44k • written 14.2 years ago by
Yuri
★ 1.7k
89
votes
31
replies
14k
views
23 follow
Best Graphics Gallery Or Blogs For Bioinformatics Use
graphs
visualization
genomics
updated 22 months ago by
Ram
44k • written 12.2 years ago by
John
★ 1.5k
88
votes
24
replies
5.6k
views
10 follow
Forum:
How To Be Helpful As A Biostar Moderator/Editor?
biostars
updated 15 months ago by
Ram
44k • written 11.2 years ago by
aidan-budd
1.9k
88
votes
24
replies
9.8k
views
14 follow
Project Euler Equivalent For Bioinformatics?
online
updated 7.3 years ago by
Biostar
20 • written 13.9 years ago by
Gabriel Mitchell
▴ 520
88
votes
36
replies
40k
views
15 follow
1000 Genomes Ld Calculation
genome
linkage
gwas
updated 10.2 years ago by
Leandro Lima
▴ 970 • written 13.6 years ago by
Ryan D
★ 3.4k
88
votes
32
replies
9.7k
views
11 follow
Forum:
Vintage / unconventional pictures for Bioinformatics
history
picture
updated 2.2 years ago by
Ram
44k • written 9.6 years ago by
Pierre Lindenbaum
161k
87
votes
24
replies
24k
views
17 follow
Tutorial:
Gene Set Enrichment Analysis
microarray
enrichment
RNA-Seq
GSEA
updated 4 weeks ago by
Gordon
• 0 • written 3.6 years ago by
Hamid Ghaedi
3.2k
87
votes
12
replies
56k
views
9 follow
What are chimeric reads?
sequence
updated 2.3 years ago by
Ram
44k • written 9.6 years ago by
zhangz.cs
▴ 300
87
votes
37
replies
52k
views
20 follow
Venn/Euler Diagram Of Four Or More Sets
r
updated 2.2 years ago by
Ram
44k • written 10.8 years ago by
Hunter
▴ 110
87
votes
25
replies
52k
views
21 follow
How To Split A Multiple Fasta
fasta
split
updated 2.3 years ago by
Ram
44k • written 13.8 years ago by
Gvj
▴ 470
87
votes
11
replies
19k
views
8 follow
Good Gwas Papers / Resources
gwas
literature
snp
updated 13.8 years ago by
Larry_Parnell
16k • written 13.8 years ago by
brentp
24k
87
votes
2
replies
59k
views
6 follow
Tutorial:
How To Ask Good Questions On Technical And Scientific Forums
how-to
updated 14 months ago by
Ram
44k • written 10.9 years ago by
Eric Normandeau
11k
86
votes
29
replies
10.0k
views
9 follow
Forum:
What is the future of EMBL-EBI (and European bioinformatics in general) in case of a 'Brexit'?
EU
brexit
EBI
politics
updated 13 months ago by
Ram
44k • written 8.0 years ago by
Michael
54k
85
votes
16
replies
97k
views
9 follow
Number of mapped reads from BAM file
bam
sam
reads
mapping
updated 18 months ago by
bhanu.chandra1
▴ 50 • written 9.1 years ago by
Prakki Rama
★ 2.7k
85
votes
46
replies
9.3k
views
15 follow
Tool:
ChatGPT optimized for bioinformatics questions
gpt
updated 9 weeks ago by
LauferVA
4.2k • written 11 months ago by
Sasha
▴ 850
85
votes
19
replies
44k
views
17 follow
How To Assess The Quality Of An Assembly? (Is There No Magic Formula?)
assembly
quality
next-gen
updated 2.3 years ago by
WANG
▴ 10 • written 11.3 years ago by
diltsjeri
▴ 470
85
votes
49
replies
10k
views
31 follow
Which Application Is Truly Missing In Bioinformatics?
subjective
general
updated 6 months ago by
Ram
44k • written 14.2 years ago by
Jarretinha
3.4k
84
votes
36
replies
19k
views
13 follow
Forum:
Benchmark - Comparison of Different NGS Mappers
mRNA-Seq
benchmark
NGS-mapper
updated 2.2 years ago by
Ram
44k • written 9.4 years ago by
David Langenberger
11k
84
votes
22
replies
4.6k
views
12 follow
Forum:
is fortran still used in bioinformatics?
FORTRAN
R
updated 14 months ago by
Ram
44k • written 4.9 years ago by
Manoj
▴ 190
84
votes
29
replies
32k
views
20 follow
Tutorial:
How to create a mutation landscape (waterfall) plot with GenVisR
BioConductor
GenVisR
Graphics
R
updated 10 months ago by
amziepickles
• 0 • written 8.2 years ago by
Obi Griffith
20k
83
votes
34
replies
19k
views
22 follow
Tutorial:
Enrichment Analysis, Clustering and Scoring with pathfindR
enrichment
R
pathway
active-subnetwork
updated 12 months ago by
adelheidkratzer
• 0 • written 5.9 years ago by
egeulgen
★ 1.3k
83
votes
20
replies
20k
views
17 follow
How Do Pathway Databases Compare?
pathway
database
subjective
updated 10 months ago by
Peter Karp
▴ 30 • written 13.5 years ago by
Shigeta
▴ 470
83
votes
30
replies
6.8k
views
18 follow
Forum:
Optimal design for bioinformatics "lab" space?
design
lab-space
updated 23 months ago by
Ram
44k • written 9.1 years ago by
Casey Bergman
18k
82
votes
27
replies
21k
views
12 follow
ATAC-seq sample normalization
ChIP-Seq
atac-seq
normalization
updated 6 weeks ago by
sgv
• 0 • written 4.4 years ago by
Flo
▴ 250
82
votes
19
replies
13k
views
8 follow
Future Directions In Bioinformatics
subjective
career
updated 15 months ago by
Ram
44k • written 13.5 years ago by
Hranjeev
★ 1.5k
82
votes
32
replies
7.8k
views
13 follow
Forum:
Shall We Go Back To Stackexchange?
meta
updated 15 months ago by
Ram
44k • written 11.5 years ago by
14134125465346445
★ 3.6k
82
votes
13
replies
41k
views
7 follow
Ti/Tv Ratio Confirms Snp Discovery. Is This A General Rule?
snp
snp
updated 3.7 years ago by
Biostar
20 • written 13.4 years ago by
Yahan
▴ 210
82
votes
11
replies
30k
views
10 follow
Qiime Vs Mothur : Why Use One Over The Other?
metagenomics
updated 7.7 years ago by
shizhaojingszj
• 0 • written 10.2 years ago by
amcrisan
▴ 370
82
votes
27
replies
54k
views
23 follow
Renaming Entries In A Fasta File
fasta
updated 8.1 years ago by
noirot.celine
▴ 50 • written 11.7 years ago by
thiago84naka
▴ 130
81
votes
8
replies
8.6k
views
6 follow
Tutorial:
Current Topics in Genome Analysis 2014 (collection of educational lectures)
workshop
lectures
genomics
training
updated 14 months ago by
Ram
44k • written 10.0 years ago by
Malachi Griffith
20k
117,059 results • Page
4 of 2342
Recent Votes
Answer: Question about samtools view flags (paired reads vs. properly paired reads)
Answer: Functional enrichment analysis for unique gene IDs
Comment: Super ehancers
Comment: Why does assigning genes with biomart give me different values than using a tran
Comment: Multiplexing for pooled CRISPR screen sequencing
Comment: How can we convert a vcf to fasta, so that I can blast some genes against that w
Answer: Differential Expression with limma: Contrast and Design Matrix, combinatorial ap
Recent Locations •
All
United States,
just now
United States,
just now
Germany,
just now
United States,
1 minute ago
United States,
1 minute ago
United States,
2 minutes ago
United States,
3 minutes ago
Recent Awards •
All
Scholar
to
Ashutosh Pandey
12k
Student
to
Holly
▴ 30
Popular Question
to
O.rka
▴ 720
Popular Question
to
natasha.sernova
★ 4.0k
Popular Question
to
Picasa
▴ 640
Supporter
to
Mol
• 0
Popular Question
to
Rafael Soler
★ 1.2k
Recent Replies
Comment: Question about samtools view flags (paired reads vs. properly paired reads)
by
Pierre Lindenbaum
161k
> Thanks. What determines what constitutes a good distance? I think tools like BWA use the median distance for each chunk of processed d…
Comment: Where are the illumina adapters on Trimmomatic take from?
by
bioinfo
▴ 150
Thank you for the explanation. Is it more appropriate then to use the sequence from the illumina documentation or the one from the github?
Comment: Question about samtools view flags (paired reads vs. properly paired reads)
by
mrk
• 0
Thanks. What determines what constitutes a good distance? I have some targeted panel data and looking at flagstat, almost all reads are pai…
Comment: Where are the illumina adapters on Trimmomatic take from?
by
GenoMax
142k
Illumina indexed adapters share a core sequence at the beginning which is what is indicated in your link. Trimming programs will remove seq…
Comment: Allele count of 2 for homoplasmic MT variants in VCF
by
Ram
44k
Please stop using `bioinformatics` as a tag unless your post is about the field of bioinformatics itself.
Comment: Multiplexing for pooled CRISPR screen sequencing
by
GenoMax
142k
> If we switch to pair-end sequencing with dual-index barcode You can use dual-indexing with single end reads. You don't need to do paired…
Comment: Super ehancers
by
jared.andrews07
★ 17k
That is not valid GFF format, so that is probably going to be problematic. See the [GFF spec](https://github.com/The-Sequence-Ontology/Spec…
Comment: Error in CIBERSORTx ($ operator is invalid for atomic vectors)
by
finch
• 0
Hey! I had this issue and I think I can help- could you take a screenshot of the .txt file opened in the notepad?
Answer: Is it possible to run CibersortX with a very small number of samples?
by
finch
• 0
Hi! I had a very similar issue and it's likely not at all related to your number of samples but to the format of your mixture file! Ciberso…
Comment: How can we convert a vcf to fasta, so that I can blast some genes against that w
by
Ram
44k
You cannot *convert* a VCF to a FASTA as they have vastly different information content. Please explain your process - what exactly do you …
Comment: Comparing Analysis Strategies for scRNA-seq Data: Separate vs. Merged Analysis o
by
Shukai
• 0
Thank you Ram for the comments! Noted for future posts.
Comment: Where to find old version of GATK best practice
by
Zhenyu Zhang
★ 1.2k
I am looking for diagrams, and step-by-step instructions of how to build my pipelines. I think the github is only helpful if you are a wdl…
Comment: Where to find old version of GATK best practice
by
Zhenyu Zhang
★ 1.2k
oh, this is helpful. Thanks.
Comment: Comparing Analysis Strategies for scRNA-seq Data: Separate vs. Merged Analysis o
by
Ram
44k
1. You can edit your post and add relevant details. There is no need to add separate comments with relevant details especially when it's no…
Answer: Functional enrichment analysis for unique gene IDs
by
geneontologyhelp
▴ 410
If you are looking at producing GO annotations, we currently only recommend InterProScan, and basically only for novel organisms or where t…
Traffic: 2907 users visited in the last hour
Content
Search
Users
Tags
Badges
Help
About
FAQ
Access
RSS
API
Stats
Use of this site constitutes acceptance of our
User Agreement and Privacy Policy
.
Powered by the
version 2.3.6