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1,000 results • Page
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7
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13
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689
views
7 follow
Forum:
Ideal PC configurations and operating system for bioinformatics laboratory
PC
updated 2 minutes ago by
i.sudbery
19k • written 29 days ago by
Estevão
▴ 10
0
votes
0
replies
199
views
Forum:
Improvement on automatic annotation of single-cell RNA-seq data from the literature using LLM
annotation
scRNA
llm
4 weeks ago by
yxwucq
• 0
5
votes
2
replies
388
views
Forum:
Bioinformatics: Where do I start
Sequencing
updated 5 weeks ago by
Ram
44k • written 5 weeks ago by
Adi
▴ 20
7
votes
7
replies
2.2k
views
Forum:
Bioinformatician who was retaliated against after reporting discriminatory harassment - Please help me navigate getting out of the situation I'm in
Career
advice
updated 5 weeks ago by
Dunois
★ 2.5k • written 5 weeks ago by
AdviceSeeker
• 0
1
vote
2
replies
416
views
Forum:
VCF bulk handling
bcftools
SnpEff
WGS
VEP
VCF
5 weeks ago by
Javier
• 0
16
votes
10
replies
1.2k
views
6 follow
Forum:
F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 7 weeks ago by
Madelaine Gogol
5.3k • written 7 weeks ago by
Ann
★ 2.4k
1
vote
1
reply
254
views
Forum:
Can I use this community for software development?
software-development
updated 8 weeks ago by
Ram
44k • written 8 weeks ago by
avpuzanov
• 0
5
votes
2
replies
417
views
Forum:
Can questions and answers on biostars be asked only in English? Can't I use Russian for example?
language
updated 8 weeks ago by
Michael
54k • written 8 weeks ago by
avpuzanov
• 0
0
votes
0
replies
200
views
Forum:
Field of Study in Computational Biology
Genomics
Computational-Biology
PhD
updated 9 weeks ago by
Ram
44k • written 9 weeks ago by
Mohammed
• 0
1
vote
3
replies
524
views
Forum:
Development plan for a bioinformatics database developer/coder
developer
9 weeks ago by
pixie@bioinfo
★ 1.5k
7
votes
8
replies
828
views
Forum:
Bioinformatics career transition
career
forum
updated 9 weeks ago by
jared.andrews07
★ 17k • written 9 weeks ago by
jevill17
▴ 20
4
votes
2
replies
546
views
Forum:
Exploring Amino Acid Patterns in Proteins Through N-gram Analysis
protein
CAFA5
10 weeks ago by
Anatoly A.
▴ 10
6
votes
6
replies
828
views
Forum:
You have 3 days of zero obligations and expansive resources. What are you building?
hypothetical
thought-experiment
11 weeks ago by
jared.andrews07
★ 17k
0
votes
0
replies
205
views
Forum:
What other data types and pipelines you would like to see from the Genomics Data Commons (GDC)
gdc
tcga
genomics-data-commons
updated 11 weeks ago by
Ram
44k • written 11 weeks ago by
Zhenyu Zhang
★ 1.2k
0
votes
2
replies
455
views
Forum:
What's the state of the art in bacterial whole genome alignment?
whole
genome
alignment
mauve
11 weeks ago by
Dave Carlson
★ 1.7k
33
votes
12
replies
1.2k
views
7 follow
Forum:
Nextflow and self-made pipelines opinion
nextflow
pipeline
data-analysis
12 weeks ago by
karlaarz
▴ 110
5
votes
4
replies
621
views
Forum:
Would reaching out to local labs and startups help with my career transition?
career
volunteering
updated 3 months ago by
GenoMax
142k • written 3 months ago by
Mango
▴ 10
1
vote
2
replies
409
views
Forum:
Exploring Career Reentry: Challenges and opportunities for Women After Maternity and Childcare Breaks
Maternity
Career-Gap
career-reentry
updated 3 months ago by
ATpoint
82k • written 3 months ago by
ashaneev07
▴ 20
8
votes
7
replies
899
views
6 follow
Forum:
Tower Server configuration for NGS data analysis
NGS
updated 3 months ago by
emmanouil.a
▴ 120 • written 3 months ago by
harasharan
• 0
1
vote
0
replies
308
views
Forum:
list of bioinformatics tools in cancer genomics
mutations
CNA
cancer
genomics
LOH
updated 3 months ago by
Pierre Lindenbaum
162k • written 3 months ago by
Bogdan
★ 1.4k
4
votes
2
replies
411
views
Forum:
bulk seq vs. sc-seq
scATAC-seq
ATAC-seq
scRNAseq
RNAseq
updated 3 months ago by
dsull
★ 6.1k • written 3 months ago by
SebKittelmann
▴ 10
8
votes
6
replies
2.9k
views
Forum:
Demonstrating Exponential Growth In Pre-School
education
written 10.3 years ago by
Asaf
10k
0
votes
0
replies
219
views
Forum:
tutorial for carrier screening analyses?
carrier-screening
updated 3 months ago by
Ram
44k • written 3 months ago by
Akos
▴ 20
2
votes
3
replies
464
views
Forum:
My Project
Project
CLI
MD-Simulations
Molecular-docking
3 months ago by
Ajay A Shettigar
• 0
17
votes
11
replies
1.1k
views
Forum:
How to overcome the imposter syndrome in bioinformatics?
advice
4 months ago by
sil_bioinfo
▴ 40
10
votes
7
replies
2.3k
views
Forum:
How to learn bioinformatics?
learning
updated 4 months ago by
Samuel Lampa
★ 1.3k • written 2.0 years ago by
sakichwan
▴ 20
26
votes
27
replies
2.2k
views
10 follow
Forum:
Determining computer time required
computer
hpc
time
updated 4 months ago by
dsull
★ 6.1k • written 4 months ago by
Rozita
▴ 40
7
votes
6
replies
788
views
Forum:
where to work as a bioinformatician (NGS/IA)
employment
omics
ia
ngs
updated 4 months ago by
Ram
44k • written 4 months ago by
Petur
▴ 20
21
votes
12
replies
3.7k
views
Forum:
How to Use Biostars Part-3: Formatting Text and Using GitHub Gists
meta
how-to
documentation
4 months ago by
Ram
44k
2
votes
1
reply
472
views
Forum:
Cataloguing available spatial transcriptomics tools, then linking them together into a workflow?
cytassist
transcriptomics
nanostring
xenium
visium
4 months ago by
LauferVA
4.2k
49
votes
20
replies
2.1k
views
10 follow
Forum:
First experiences matter
first-time
tolerance
updated 4 months ago by
Michael Love
★ 2.6k • written 4 months ago by
Mensur Dlakic
★ 27k
4
votes
3
replies
828
views
Forum:
How to get started in bioinformatics
students
updated 4 months ago by
Ram
44k • written 4 months ago by
Soidihane
▴ 10
2
votes
2
replies
610
views
Forum:
Do batches contain biological variations in omics data (RNAseq or scRNAseq)?
combat
single-cell
omics
batch
rnaseq
updated 4 months ago by
Ram
44k • written 4 months ago by
cwwong13
▴ 40
0
votes
2
replies
3.3k
views
Forum:
I’m considering a career transition from software engineering to bioinformatics. Is it a good fit for me?
career
updated 4 months ago by
GenoMax
142k • written 4 months ago by
Mango
▴ 10
5
votes
3
replies
655
views
Forum:
Binning the rare biosphere
metagenomics
soil
assembly
techniques
binning
updated 5 months ago by
Mensur Dlakic
★ 27k • written 5 months ago by
Konstantin
▴ 20
6
votes
2
replies
698
views
Forum:
how to start Bioinformatics?
basics
updated 5 months ago by
Mensur Dlakic
★ 27k • written 5 months ago by
sajibsuvo70
• 0
19
votes
5
replies
1.2k
views
6 follow
Forum:
What Are The Most Influential Bioinformatics/Computational Biology Papers Of 2023?
genomics
snps
survey
papers
5 months ago by
Raony Guimarães
★ 1.4k
4
votes
6
replies
996
views
6 follow
Forum:
Bioinformatics: what do you think of it in the next 20 years?
future
updated 5 months ago by
Ram
44k • written 5 months ago by
Daniel
• 0
1
vote
1
reply
519
views
Forum:
want to work in open source project/project in bioinformatics
project
updated 5 months ago by
Ram
44k • written 5 months ago by
Afeef
• 0
3
votes
5
replies
1.1k
views
Forum:
Current Trends in Bioinformatics
structural-bioinformatics
updated 5 months ago by
Ram
44k • written 6 months ago by
Paulo
• 0
21
votes
11
replies
2.6k
views
6 follow
Forum:
Rust bioinformatics projects ideas
projects
scRNA-seq
RNA-seq
Rust
updated 6 months ago by
Jeremy Leipzig
22k • written 14 months ago by
bompipi95
▴ 150
176
votes
46
replies
19k
views
26 follow
Forum:
Crac: Funny And/Or Weird Names For Bioinformatics Tools
humor
6 months ago by
Istvan Albert
100k
3
votes
2
replies
650
views
Forum:
RNA-Seq data analysis - question
Python
RNA-Seq
R
updated 6 months ago by
fturner
▴ 10 • written 6 months ago by
sil_bioinfo
▴ 40
0
votes
0
replies
395
views
Forum:
How to learn bioinformatics online?
self-education
education
learning
updated 6 months ago by
Ram
44k • written 6 months ago by
Brian Rey | BioLearn
• 0
6
votes
5
replies
2.3k
views
Forum:
Maintaning internal or private installation of Biostar or a similar Q&A site
Biostars
6 months ago by
tshtatland
▴ 190
7
votes
9
replies
1.5k
views
6 follow
Forum:
Mentor for a beginner bioinformatics student
project
mentor
6 months ago by
UT_FAN
▴ 40
0
votes
0
replies
489
views
Forum:
Steps after doublet detection in single cell data
scRNA
doublet
7 months ago by
ishwarvh
• 0
0
votes
0
replies
376
views
Forum:
Automated Checks And QA
retrospective
7 months ago by
Sasha
▴ 850
7
votes
13
replies
1.4k
views
Forum:
How do you validate and verify your pipeline's software updates?
Validation
Verification
Quality-Assurance
updated 7 months ago by
Ram
44k • written 7 months ago by
kieran
▴ 10
21
votes
7
replies
12k
views
Forum:
Bioinformatics short paper journal resource [software/webapps/database]
journal
software
webapp
updated 7 months ago by
Gennady Khvorykh
▴ 100 • written 8.1 years ago by
Sukhi Singh
11k
1,000 results • Page
1 of 20
Recent Votes
Comment: log2 fold change in RNA-seq analysis
Comment: log2 fold change in RNA-seq analysis
Answer: Ideal PC configurations and operating system for bioinformatics laboratory
correct way of analyzing cell proportions in singlecell data
Comment: How to interpret this plotMDS of three disease clusters?
gseGO: no term enriched under specific pvalueCutoff
Answer: Error with BiocParallel. No barcodes files found
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Recent Replies
Answer: Z score
by
Akash D
▴ 50
I made this. Z scored on log2FC? will anyone support me on this? 3 groups A,B,C
Comment: log2 fold change in RNA-seq analysis
by
i.sudbery
19k
There is no such thing as a fold change of -2. Fold change is expression_in_condition_A/expresssion_in_condition_B as expression is a…
Comment: Ideal PC configurations and operating system for bioinformatics laboratory
by
i.sudbery
19k
DE with 100s of samples (particulalry if your experiemental design is more complex than 2 condition DE) can stretch consumer grade hardware…
Comment: Error with BiocParallel. No barcodes files found
by
NTerway
• 0
Thank you for your help! I will try this out. I am trying to implement a collaborator's pipeline on my data and they only use SCE, scran an…
Comment: Ideal PC configurations and operating system for bioinformatics laboratory
by
ATpoint
82k
Once you have the counts any n is possible on a regular analysis-grade laptop or workstation. Even hundreds of samples. It's really the pre…
Comment: Error with BiocParallel. No barcodes files found
by
ATpoint
82k
You just tell the function to remove the prefix for you. Not on the actual file. Then you can add this information to the SCE, like `sce$da…
Comment: log2 fold change in RNA-seq analysis
by
May Ling
• 0
Thank you for your response. I apologize if my understanding is incomplete. I would like to ask: if I have a fold change of -2, how can I c…
Comment: Ideal PC configurations and operating system for bioinformatics laboratory
by
i.sudbery
19k
Yeah, a standard 3x3 bulk experiement is very analysable on pretty much most good consumer laptops these days.
Comment: Error with BiocParallel. No barcodes files found
by
NTerway
• 0
Thanks for the explanation! If I remove the prefix (GSM3972009_69_) then I will lose the sample annotation and cannot track them. I am more…
Comment: Unexpected read length from NGS
by
ATpoint
82k
Seconding this. Just ask them.
Answer: Error with BiocParallel. No barcodes files found
by
ATpoint
82k
The function assumes simply barcodes.tsv(.gz), genes.tsv(.gz) and matrix.mtx(.gz), without additional pre- or suffixes by default. You can …
Comment: Free AI for R programming
by
GenoMax
142k
Try: https://ai.tinybio.cloud/
Comment: How to interpret this plotMDS of three disease clusters?
by
ATpoint
82k
arrayWeights is imo always a good idea with human (or generally large) cohorts. What you can also do is to use something like sva to estima…
Comment: Cellranger-multi : Demultiplexing and Analyzing 5’ Immune Profiling Libraries Po
by
GenoMax
142k
Not what you want to hear but it is possible that the Ab data is not good quality as the cellranger message says (assuming everything ran p…
Comment: Ideal PC configurations and operating system for bioinformatics laboratory
by
ATpoint
82k
Bulk RNA-seq is way less intensive in every regard than single-cell. Whatever you buy, be sure it's upgradable, meaning, no Mac :)
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