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120,988 results • Page
1 of 2420
Sort: Rank
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0
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Detection SNPs and Indels Bacteria
SNPs
46 minutes ago by
Eltercertopico
• 0
0
votes
3
replies
98
views
Insight into Trimmomatic
Trimmomatic
Trimming
Reads
updated 2 hours ago by
GenoMax
150k • written 8 hours ago by
jh5ym
• 0
0
votes
2
replies
110
views
Error during running wf-bacterial-genome (nextflow run epi2me-labs/wf-bacterial-genomes).
EPI2ME
MEDAKA
updated 8 hours ago by
Ram
45k • written 10 hours ago by
Anurag
• 0
836
votes
167
replies
173k
views
111 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 2 days ago by
Biostar
3.5k • written 8.4 years ago by
Istvan Albert
102k
1
vote
1
reply
101
views
Why don't I see people calculating FPKM from normalized read counts?
normalization
RNA-seq
updated 9 hours ago by
Istvan Albert
102k • written 12 hours ago by
shelkmike
★ 1.5k
0
votes
2
replies
106
views
Trouble when uploading FASTQ files on NCBI
rnaseq
ncbi
updated 8 hours ago by
Ram
45k • written 13 hours ago by
Gerard
▴ 10
0
votes
2
replies
111
views
SyntaxError in file /snakefile, line 22: invalid decimal literal: None
python
snakemake
updated 8 hours ago by
Ram
45k • written 14 hours ago by
Ana
• 0
3
votes
2
replies
182
views
News:
Building an mmCIF parser for enzyme structure analysis — feedback & ideas welcome!
mmcif
11 hours ago by
Shravya
• 0
0
votes
0
replies
77
views
News:
Course: Introduction to Genome Annotation (Online, 19–22 May 2025)
Gene-Prediction
Genome-Annotation
Gene-Models
19 hours ago by
Physalia-courses
★ 2.6k
0
votes
1
reply
108
views
Error with running cellranger-atac count
cellranger
atac
single-cell
updated 8 hours ago by
Ram
45k • written 20 hours ago by
Khôi
• 0
0
votes
1
reply
119
views
HIC-pro with Forward and reverse reads not paired even when I use their test data.
HIC-pro
updated 14 hours ago by
GenoMax
150k • written 1 day ago by
Aki
▴ 20
0
votes
1
reply
279
views
Using DEXseq on kallisto tsv outputs ?
alignement
DEXseq
kallisto
updated 1 day ago by
Gordon Smyth
★ 7.9k • written 6 days ago by
Gerard
▴ 10
0
votes
0
replies
109
views
Job:
2025 Arrowhead AI/LLM for Computational Biology and Data Science Internship
intern
updated 1 day ago by
GenoMax
150k • written 1 day ago by
Shicheng Guo
★ 9.6k
1
vote
2
replies
475
views
ChIP-seq gene blacklist for Rattus norvegicus
rat
blacklist
ChIP-seq
updated 1 day ago by
GenoMax
150k • written 12 months ago by
Thomas
• 0
4
votes
10
replies
787
views
6 follow
Extract length of sample sequence from vcf
microsatellites
bcftools
vcf
indels
variants
updated 1 day ago by
cmdcolin
★ 4.2k • written 11 days ago by
jahnreinhard.ringger
• 0
0
votes
0
replies
106
views
Tutorial:
Fast SCENIC GRN Analysis with RegDiffusion
GRN
network
scRNA-seq
wgcna
1 day ago by
Hao
▴ 10
2
votes
3
replies
549
views
Is it reasonable to do "Gene Regulatory Network Inference" for each cell type and do differential GRN
GRN
scRNA-seq
updated 1 day ago by
Hao
▴ 10 • written 2.6 years ago by
shangguandong1996
▴ 30
1
vote
2
replies
308
views
Blastx issue
blastx
lncRNA
updated 8 hours ago by
Ram
45k • written 4 days ago by
Avik
• 0
2
votes
9
replies
518
views
TPM vs FPKM divergence at high values
tpm
fpkm
normalisation
updated 12 hours ago by
Istvan Albert
102k • written 3 days ago by
megancgrdnr
• 0
3
votes
0
replies
123
views
Herald:
The Biostar Herald for Monday, April 14, 2025
herald
1 day ago by
Biostar
3.5k
0
votes
2
replies
270
views
What is the best way to know if a pair of genes is random?
go-terms
genes
updated 8 hours ago by
Ram
45k • written 4 days ago by
Ana
• 0
1
vote
1
reply
214
views
How to save the AlphaFold PAE plot as an image in ChimeraX using command line ?
PAE
ChimeraX
AlphaFold
updated 1 day ago by
LauferVA
4.7k • written 3 days ago by
Picasa
▴ 680
1
vote
0
replies
96
views
News:
LAST CALL - Bioinformatics Pipeline Development with Nextflow - May 19-21, 2025 (online) - Apply Now
nextflow
rnaseq
workshop
1 day ago by
ecSeq Bioinformatics
▴ 20
1
vote
2
replies
225
views
RNA-seq, three comparisons - stick to one linear model, or use separate models?
statistics
mixed-effect-model
stats
RNAseq
linear-model
updated 8 hours ago by
Ram
45k • written 2 days ago by
noodlejackson
▴ 40
1
vote
1
reply
142
views
Using Principal Components may muddle the detecting power of a GWAS?
q-value
covariates
genomic-inflation
pca
gwas
updated 1 day ago by
DBScan
▴ 480 • written 1 day ago by
AndrMod
• 0
0
votes
0
replies
112
views
Use Seed or Full Alignments from Pfam HMM as a filter step
hmm
sequences
metagenomic
1 day ago by
Janelyn123
• 0
3
votes
4
replies
278
views
Helicobacter pylori low-rate alignment
RNA-seq
transcriptomics
updated 8 hours ago by
Ram
45k • written 2 days ago by
Ariadna
▴ 20
14
votes
3
replies
11k
views
Can The Wget Command Be Used To Download Pdbs
pdb
updated 1 day ago by
Calvin
• 0 • written 13.5 years ago by
Harpalss
▴ 310
0
votes
3
replies
299
views
Is a log2 transformation an essential step in preparing expression data for machine learning?
Machine-learning
preproccessing
microarray
updated 8 hours ago by
Ram
45k • written 2 days ago by
Sib
▴ 60
0
votes
2
replies
883
views
How to re-annotate the structure of gene against a known genomic file
RNA-Seq
updated 2 days ago by
Mensur Dlakic
★ 29k • written 5.9 years ago by
Kanoo
• 0
0
votes
2
replies
252
views
ONT Live-Basecalling Issue?
ONT
minKNOW
live-basecalling
updated 22 hours ago by
LauferVA
4.7k • written 3 days ago by
Akshay
• 0
3
votes
4
replies
298
views
Identify gene from sequence at scale in non-model organisms
sequence
gene
updated 8 hours ago by
Ram
45k • written 3 days ago by
matthewmerkin32
• 0
0
votes
0
replies
158
views
News:
Cellosaurus release 52 is available
biocuration
database
knowledgebase
cell-line
updated 8 hours ago by
Ram
45k • written 3 days ago by
Amos Bairoch
▴ 130
0
votes
2
replies
290
views
WGCNA
WGCNA
updated 8 hours ago by
Ram
45k • written 4 days ago by
香
• 0
0
votes
0
replies
303
views
Seeking Advice on Causal Inference for Treatment Effect Prediction (Small Sample, Genomic Covariates)
causal-inference
updated 8 hours ago by
Ram
45k • written 3 days ago by
oghzzang
▴ 50
5
votes
5
replies
464
views
Batch effect
RNAseq
Batch-Effect
2 days ago by
Fatemeh
▴ 40
0
votes
4
replies
401
views
convert Gene ID to Gene Symbol
gene-id
updated 3 days ago by
pinheirofabiano
▴ 110 • written 6 days ago by
majorprogeria
• 0
0
votes
1
reply
226
views
News:
Structural bioinformatics workshop
Structural-bioinformatics
updated 8 hours ago by
Ram
45k • written 4 days ago by
reetesh
• 0
1
vote
10
replies
598
views
How to test the similarity of two gene expression profiles
gene-sets
updated 8 hours ago by
Ram
45k • written 5 days ago by
htchd
• 0
3
votes
3
replies
262
views
Which are the different kinds of GeneID that exist?
R
RNA-Seq
4 days ago by
pinheirofabiano
▴ 110
0
votes
0
replies
174
views
Error using DWLS
deconvolution
DWLS
RNAseq
4 days ago by
Ana
• 0
0
votes
5
replies
1.7k
views
How to remove this module selection error?
WGCNA
updated 4 days ago by
香
• 0 • written 2.3 years ago by
gupta00aritra
• 0
0
votes
1
reply
1.1k
views
bbsplit : How to configure ambiguous/ambiguous2 parameters in order to consider as unmapped reads, reads mapped on both human/mouse references.
bbsplit
nextflow
updated 4 days ago by
GenoMax
150k • written 4 days ago by
ZheFrench
▴ 590
1
vote
2
replies
252
views
Limma for DEG analysis
R
DEG
limma
updated 8 hours ago by
Ram
45k • written 4 days ago by
rahma.khoualdia
• 0
0
votes
0
replies
217
views
deletion impact
vcf
updated 4 days ago by
GenoMax
150k • written 4 days ago by
HarperReed
• 0
0
votes
2
replies
270
views
Help finding GTEx v8 file
GTEx
SHAPEIT2
3 days ago by
蒲蒲
• 0
2
votes
5
replies
323
views
Idat file processing issue with command line cli
CanineHTS
IDAT
4 days ago by
1769mkc
★ 1.2k
0
votes
0
replies
178
views
is there a way to get --ref paths to prioritize my order with vg call?
call
vg
vgcall
1 day ago by
PolenP
• 0
0
votes
1
reply
239
views
Has anyone tried used simple ML models to identify virulence genes?
genetics
R
classifier
genomics
machinelearning
updated 4 days ago by
Mensur Dlakic
★ 29k • written 5 days ago by
Jack
• 0
0
votes
4
replies
360
views
What does vg autoindex mpmap do?
vg
mpmap
autoindex
1 day ago by
Ziyue
• 0
120,988 results • Page
1 of 2420
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error with CombineGVCFs to join large number of files
Counting number of reads and bases fastQ with python
Answer: Whole transcriptome analysis nominated primarily miRNA-related pathways. Advice?
Comment: Using Principal Components may muddle the detecting power of a GWAS?
Answer: Building an mmCIF parser for enzyme structure analysis — feedback & ideas welcom
Why don't I see people calculating FPKM from normalized read counts?
Comment: makeblastdb Bus error
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Recent Replies
Comment: Insight into Trimmomatic
by
GenoMax
150k
You can post screenshots of plots you have questions about.
Comment: Insight into Trimmomatic
by
jh5ym
• 0
Follow up question, how do I share the .html file here to show you the Multi QC report?
Comment: Insight into Trimmomatic
by
GenoMax
150k
It would definitely be useful to first run FastQC/MultiQC on your data to understand the overall characteristics of the data. Make a note o…
Comment: Error during running wf-bacterial-genome (nextflow run epi2me-labs/wf-bacterial-
by
Anurag
• 0
hello I am very new to this field, I am trying to assemble genome using wf-bacterial-genome (nextflow run epi2me-labs/wf-bacterial-genomes…
Comment: Error during running wf-bacterial-genome (nextflow run epi2me-labs/wf-bacterial-
by
GenoMax
150k
Please add some detail about the command you are running and what exactly you are trying to do. You may have put part of the command line i…
Comment: Building an mmCIF parser for enzyme structure analysis — feedback & ideas welcom
by
Shravya
• 0
Thank you so much for the clarification! You're right — I should've phrased it more as building a parser "for mmCIF files" in general, and…
Comment: Why don't I see people calculating FPKM from normalized read counts?
by
Istvan Albert
102k
Scientists stopped using FPKM in general because better measures are available: https://www.biostars.org/p/104214/#332357 > *The Total Co…
Comment: Trouble when uploading FASTQ files on NCBI
by
lieven.sterck
15k
Not solving this particular problem but merely a suggestion: Given your 'location' you might also consider uploading data to ENA / arrayEx…
Comment: SyntaxError in file /snakefile, line 22: invalid decimal literal: None
by
Jesse
▴ 860
That's [Python's f-string](https://docs.python.org/3/tutorial/inputoutput.html#formatted-string-literals), which in this case just turns `{…
Comment: TPM vs FPKM divergence at high values
by
Istvan Albert
102k
I admit I had no firm idea what a TPM vs FPKM plot might look like. I never computed both, but this motivated me enough to take a salmon …
Comment: Trouble when uploading FASTQ files on NCBI
by
GenoMax
150k
This may be hard to help/diagnose for biostar community since we don't know about your local set up. Problem could be on either end (your o…
Comment: TPM vs FPKM divergence at high values
by
dariober
15k
Yes, the axes are both logarithmic, the difference is in the input data with FPKM being log'd and TPM being raw.
Comment: SyntaxError in file /snakefile, line 22: invalid decimal literal: None
by
ATpoint
87k
I am not a SnakeMake person but what is the `f` supposed to be? `f'/{{...`
Comment: TPM vs FPKM divergence at high values
by
Istvan Albert
102k
I think both are logarithmic axes. Just one is in exponential notation the other is not.
Comment: HIC-pro with Forward and reverse reads not paired even when I use their test dat
by
GenoMax
150k
Looks like the original dataset has an equal number of records based on the lines so that input looks correct. $ wc -l SRR400264_* …
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