This is a test version of Biostars. For the public version, visit https://www.biostars.org.
2 votes
4 replies
1.1K views
miRNA Quantifiaction Tool
quatification mirna
updated by GenoMax GenoMax • written by anthony.santana.703.j • anthony.santana.703.j
0 votes
3 replies
1.4K views
WGCNA pickSoftThreshold problem
picksoftthreshold question problem wgcna
updated by LChart LChart • written by ton_of_questions • ton_of_questions
0 votes
1 reply
752 views
Is comparing seeds sufficient, or should alignments be compared instead?
readmapping
updated by shenwei356 shenwei356 • written by curiousfellow@123 • curiousfellow@123
3 votes
2 replies
1.3K views
How to download protozoa reference genomes from NCBI
genomes ncbi
updated by colindaven colindaven • written by anna • anna
1 vote
1 reply
837 views
Clustering on Big data (100 million samples)
clustering data learning big machine
updated by Mensur Dlakic Mensur Dlakic • written by Rushikesh • Rushikesh
3 votes
7 replies
3.5K views
Where can I find a vcf file with individuals in it, that have a specific disease?
phenotype vcf
updated by edu123updates • edu123updates • written by Patrick • Patrick
0 votes
2 replies
1.6K views
ERROR::INVALID_TAG_NM:Record A, Read name B, NM tag (nucleotide differences) in file [0] does not match reality [1]
bwa
written by curious curious
2 votes
11 replies
3.4K views
Cut&Run TF Analysis - Very Low Peak Counts Despite Good Library Quality
macs2 cutandrun chipseq
updated by jared.andrews07 jared.andrews07 • written by sk • sk
0 votes
0 replies
1.4K views
Job: Ensembl Genebuild Project Leader, EMBL-EBI, Hinxton near Cambridge, UK
ensembl jobs
updated by GenoMax GenoMax • written by A@Ensembl • A@Ensembl
0 votes
2 replies
846 views
tools to increase hi-c resolution
resolution hi-c
written by J.F.Jiang J.F.Jiang
2 votes
2 replies
976 views
How to fix this this tangle in my bacterial genome assembly
misassembly assembly genomics ngs
written by Mark • Mark
0 votes
4 replies
2.1K views
Help configuring RepeatMasker
human illumina libraries repeatmasker
updated by Michael Dondrup Michael Dondrup • written by María José • María José
0 votes
2 replies
789 views
How to process Iso-Seq files in SRA public fastqs
iso-seq
written by Abieskawa • Abieskawa
3 votes
19 replies
11K views
ACMG assignment tools
wes acmg wes wgs
updated by Prash • Prash • written by Eugene A Eugene A
0 votes
0 replies
649 views
Colsep for dendrogram and ColSideColors in heatmap.2
heatmap dge edger heatmap.2
written by fabian • fabian
0 votes
1 reply
880 views
Decision making on choosing the based on tblastn result
interpretation tblastn blast e-score
updated by GenoMax GenoMax • written by srestha • srestha
4 votes
7 replies
3.9K views
How to Identify the pathogenicity of a set of variants (by using ACMG guidelines)?
pathogenicity snp variants
updated by Damianos P. Melidis • Damianos P. Melidis • written by DanielC DanielC
0 votes
4 replies
1.6K views
Why am I getting the exact same MarkDuplicates results when passing a query or coordinate sorted bam?
picard bwa
updated by GenoMax GenoMax • written by curious curious
0 votes
2 replies
761 views
use AverageExpression to stratify samples
scrna
written by ndra1456 • ndra1456
0 votes
0 replies
1.4K views
Multiple taxonomy hits per OTU ID in VSEARCH despite --top_hits_only (90% ID after QIIME2 clustering at 97%)
vsearch
updated by GenoMax GenoMax • written by salma.sarker • salma.sarker
0 votes
0 replies
627 views
RepeatMasker output file question
repeatmasker
written by xinguok794 • xinguok794
1 vote
1 reply
1.1K views
Which GWAS tool to use that can work on Czech cases and British controls, on a binary trait?
plink2 gwas saige
updated by LChart LChart • written by anita.szabo08 • anita.szabo08
1 vote
2 replies
1.0K views
Unsure whether or not I overcorrected for batch effect in dataset
quality plot pca control
updated by GenoMax GenoMax • written by Megan • Megan
0 votes
3 replies
1.3K views
Primer removal in amplicon sequencing
removal primer amplicon dada2 sequencing
updated by GenoMax GenoMax • written by sxf520 • sxf520
0 votes
1 reply
1.9K views
GATK BQSR error — Reference and BAM file chromosome name mismatch (“chr” vs. no “chr”)
gatk vcf bsqr
updated by Ram Ram • written by iamsmor • iamsmor
0 votes
1 reply
933 views
Batch correction without biological covariates
combat-seq rna-seq batch-correction
updated by Ram Ram • written by as823jk • as823jk
0 votes
3 replies
1.3K views
RagTag results
ngs scaffolding ragtag gaps
updated by shelkmike shelkmike • written by synthiiihuh • synthiiihuh
0 votes
2 replies
848 views
Tools that output positions of matching kmers
sequences genomics kmers
written by gil.hornung gil.hornung
0 votes
4 replies
1.2K views
mitochondrial assamply use NOVOplasty
mitochondrial genome mammles
updated by dr_cfoster dr_cfoster • written by m90 • m90
0 votes
3 replies
1.1K views
Still bad phred score after fastp
phred fastp
updated by Arup Ghosh Arup Ghosh • written by SpliceAndScript • SpliceAndScript
0 votes
1 reply
840 views
Cytohubba problem and difference networks
network cytoscape cytohubba analysis
updated by Scooter Scooter • written by mastoreh • mastoreh
0 votes
1 reply
975 views
Cytoscape KEGG plots reverting to original layout after changing style or column
kegg cytoscape
updated by Scooter Scooter • written by Adrian • Adrian
0 votes
6 replies
1.7K views
Mass-spectrometry proteomics
proteomics analysis
updated by Gordon Smyth Gordon Smyth • written by Nasim Gandomdoust • Nasim Gandomdoust
4 votes
3 replies
1.5K views
Biological meaning of metrics commonly used for QC in scRNA-seq?
ribosome qc velocity single-cell intron
updated by jared.andrews07 jared.andrews07 • written by txema.heredia • txema.heredia
0 votes
0 replies
805 views
News: Eukaryotic eDNA Metabarcoding Course (14–18 July, Online)
metabarcoding edna genomics nanopore
written by Physalia-courses Physalia-courses
1 vote
12 replies
2.5K views
miRNA low mapping(Qiagen miRNA Library Prep)
qiagen pipeline trimming alignment mirna
updated by GenoMax GenoMax • written by anthony.santana.703.j • anthony.santana.703.j
0 votes
12 replies
3.4K views
STAR alignment not detecting some transcripts / custom reference
star bwa rna-seq alignment scrna-seq
updated by rfran010 • rfran010 • written by npont • npont
0 votes
0 replies
558 views
eHOMD database: Ref seq Aligned FASTA vs Full RefSeq?
homd database
written by bioinfo bioinfo
0 votes
3 replies
1.5K views
pysam header and body
bam pysam view
updated by dariober dariober • written by Matteo Ungaro • Matteo Ungaro
2 votes
1 reply
1.1K views
Discrepency in assembly sizes
pacbio assembly kmer
updated by shelkmike shelkmike • written by hpapoli hpapoli
0 votes
1 reply
784 views
fasterq-dump fails before completing lookup
lookup incomplete fasterq-dump
updated by GenoMax GenoMax • written by nmannda148 • nmannda148
0 votes
4 replies
1.5K views
ATACSEQ normlization for bigwig files
normalization atac-seq
updated by ATpoint ATpoint • written by RD • RD
0 votes
6 replies
1.7K views
finding gene sequence from WGS data
gene wgs
updated by swbarnes2 swbarnes2 • written by Bushra Bibi Bushra Bibi
0 votes
0 replies
637 views
Question about Protein-protein-DNA complex prediction
prediction protein
written by mickley413 • mickley413
0 votes
10 replies
3.5K views
Supergnova stuck in tutorial
genetic correlations python supergnova conda
updated by s.faria • s.faria • written by Gerard • Gerard
2 votes
4 replies
1.7K views
RNAseq meta-analysis to identify “consistently expressed” genes
meta-analysis method rnaseq
updated by mbyvcm mbyvcm • written by cgibbsm • cgibbsm
1 vote
2 replies
964 views
pddg+odgi+vg to perform pangenome analysis to cyanobacteria
odgi pggb help pangenome vg
updated by GenoMax GenoMax • written by snmdba • snmdba
1 vote
2 replies
1.0K views
Comparing sets of genes for similarity
r jaccard genes marker clustering
written by yura.grabovska • yura.grabovska
0 votes
0 replies
887 views
News: Network Analysis in Systems Biology - 2 seats left
bioconductor systemsbiology r networkanalysis rnaseq
written by Physalia-courses Physalia-courses
1 vote
5 replies
1.3K views
Running Unicycler with a merged fastq file
unicycler bacterial-genome assembly
updated by GenoMax GenoMax • written by Assa Yeroslaviz Assa Yeroslaviz
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