To create amino acid substitution in protein structure

Question

[shwetamgr1]

i have 3-D structure of protein (PDB format), and in that structure i want to create an amino acid substitution at specific position of protein and after that i need to know the global minimum energy of mutated structure.

Please suggest any software regarding above problem or any method i can follow

thank you

Answer 1

[Mensur Dlakic]

Changing a single amino-acid is not that difficult (for example, see Swiss-Model or Modeller), but what you want appears to be more rigorous. I suggest FoldX or Rosetta, but beware that both of them have steep learning curves. FoldX has an explanation here and for Rosetta there are several, but you can probably start here.

[shwetamgr1]

thanx i will try it