To find amino acid substitution from fasta alignment
I have fasta multiple sequence alignment file of protein sequence. I want to look for mutation/ substitution in amino acid between query with reference to one query. Is there any R package or any method i can go through.
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https://www.applied-maths.com/tutorial/sequence-alignment-and-mutation-analysis
thanks, this method will itself align and search from the sequence. my concern is from aligned file. i have already msa file in fasta alignment format. i want to perform search on that.