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To find amino acid substitution from fasta alignment

I have fasta multiple sequence alignment file of protein sequence. I want to look for mutation/ substitution in amino acid between query with reference to one query. Is there any R package or any method i can go through.

aminoacid proteomics perl genomics r

thanks, this method will itself align and search from the sequence. my concern is from aligned file. i have already msa file in fasta alignment format. i want to perform search on that.

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