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0 votes
0 replies
617 views
Installing SALSA and HIRise scafolding software
scafolding salsa genome-assembly hirise
updated by Ram Ram • written by Lissa Cruz Saavedra • Lissa Cruz Saavedra
0 votes
1 reply
822 views
Extract Di-nucleotide/Tri-Nucleotide substitution frequencies from MSA/VCF file
msa substitution vcfstats evolution vcf
updated by Pierre Lindenbaum Pierre Lindenbaum • written by Saran • Saran
0 votes
2 replies
1.1K views
Abundance Calculation in MetaPhlAn 3: Methodology, TPM or RPKM, and the Treatment of Unclassified Reads
metaphlan normalization rpkm tpm
written by ramin.k2013 • ramin.k2013
0 votes
0 replies
541 views
Normalization in Metagenomics Sequencing By Total Number of Reads: Pre- or Post-Filtering and the Role of Unclassified Reads
normalization metagenomics
updated by Ram Ram • written by ramin.k2013 • ramin.k2013
0 votes
3 replies
1.3K views
Finding variants within a subset of a BAM file
variant calling
written by ramiro.barrantes • ramiro.barrantes
2 votes
1 reply
2.3K views
convert h5ad to rds
single-cell transcriptome
updated by bk11 • bk11 • written by lucas.aleixoleal17 • lucas.aleixoleal17
0 votes
2 replies
1.8K views
Error during demultiplexing by bcl2fastq
bcl2fastq
updated by GenoMax GenoMax • written by daffodil • daffodil
0 votes
2 replies
1.1K views
Help with generating annotation database for dog genome to use in ANNOVAR for variant annotation of dog tumor sequences
annovar
written by sainavyav22 • sainavyav22
0 votes
1 reply
1.4K views
Clair3 very low F1 INDEL score
ont clair3 nanopore wgs
updated by yussab • yussab • written by Satrio • Satrio
0 votes
9 replies
2.7K views
99.9999% of Q30 bases is normal?
rna-seq fastp
updated by LauferVA LauferVA • written by Aki • Aki
0 votes
0 replies
775 views
Issue while using circle_dat function from GOplot in R
goplot r
written by ckelly96 • ckelly96
0 votes
7 replies
6.4K views
fastp - can I trim specific adapters only?
trimming fastp adapter
written by liorglic liorglic
0 votes
2 replies
1.0K views
Can't figure out plink --sample-diff
plink
written by curious curious
0 votes
1 reply
6.8K views
How to compute a one tail (one way) log rank test for survival?
r survival hypothesis-testing log-rank
updated by jack • jack • written by denalitastic • denalitastic
0 votes
1 reply
1.1K views
Geneious Prime (Consense Sequence)
consense sequence
updated by GenoMax GenoMax • written by Reno • Reno
0 votes
2 replies
1.0K views
convert a seurat object to expressionset
seurat
written by Nona • Nona
2 votes
0 replies
529 views
News: Course Functional Programming with R
rstats tscourses rproject keeplearning rlang
written by Transmitting Science communication • Transmitting Science communication
0 votes
1 reply
671 views
Shared pathways up in disease blood and tissue relative to healthy blood (scRNA-seq)
scrna-seq
updated by ATpoint ATpoint • written by Lucy • Lucy
0 votes
4 replies
1.4K views
Per Base Sequence Content - continuous raise of G%
fastqc
updated by GenoMax GenoMax • written by pl.terzian • pl.terzian
0 votes
0 replies
724 views
Inferring cell identity/genotype in single cells with missing information
cell seurat single score
written by txema.heredia • txema.heredia
0 votes
2 replies
1.1K views
fmols for nanopore library loading
nanopore sequencing libraryprep fmol lsk114
updated by GenoMax GenoMax • written by njornet • njornet
0 votes
0 replies
382 views
cibersortx colnames
gema122
written by gem1 • gem1
2 votes
37 replies
21K views
Convert Gene IDs of Tomato Sol Database
tomato gene
updated by aziz.hammou • aziz.hammou • written by kevluv93 kevluv93
0 votes
2 replies
1.2K views
Beagle imputation
beagle
written by mahlet.teka • mahlet.teka
4 votes
2 replies
1.8K views
News: 8th Berlin Summer School in NGS Data Analysis (June 10-14, 2024) - already half booked
workshop rna-seq dna-seq illumina
written by David Langenberger David Langenberger
0 votes
2 replies
1.1K views
How to detect and visualize ORFs in bacterial genome?
statistics visualization orfs genome
written by marongiu.luigi marongiu.luigi
1 vote
13 replies
15K views
biomaRt: Timeout on getBM().
r biomart ensembl
updated by Mike Smith Mike Smith • written by lincaijin1994 • lincaijin1994
0 votes
8 replies
7.3K views
cellranger mkref failed
next-gen-sequencing software-error rna-seq
updated by Zikun-Yang • Zikun-Yang • written by Dan • Dan
0 votes
0 replies
685 views
Confused about the results of a function in dada2 pipeline sample inference step
microbiome r dada2
written by Mohamed Samir • Mohamed Samir
1 vote
4 replies
2.1K views
I want to calculate if the sample has whole genome duplication event or not?
sequencing wgs
written by Hyper_Odin Hyper_Odin
0 votes
4 replies
1.2K views
Addmetadata to seurat obj
seurat
written by synat.keam • synat.keam
2 votes
8 replies
3.5K views
Why are the basics so complicated? Basic coverage filtering for VCF files
filter vcf coverage
updated by spen • spen • written by MBarcelo • MBarcelo
0 votes
5 replies
2.1K views
NCBI MEGABLAST versus UCSC BLAT
blast blat alignment megablast
updated by noodle noodle • written by a615ebfb • a615ebfb
0 votes
0 replies
658 views
Methylation array analysis using dmpFinder
epic methylation normalization dmp mset
written by Roy • Roy
2 votes
0 replies
645 views
News: Online course: Genome Annotation
genome-assembly rna-seq genome-annotation
updated by Ram Ram • written by Physalia-courses Physalia-courses
0 votes
4 replies
1.7K views
How to use pre-alignments by bowtie2
atac-seq bowtie2
written by Aki • Aki
0 votes
0 replies
736 views
Confused by the `--ld-window` flag in Vcftools. What does the number of SNPs between SNPs mean?
tabix vcftools
written by rijan_dhakal2055 • rijan_dhakal2055
0 votes
4 replies
1.6K views
Database or Tool: Identifying Gene Regions (Exon/Intron/UTR) from Chromosome Position
gene-region biomart ucsc-browser intron exon
updated by GenoMax GenoMax • written by kelpotus22 • kelpotus22
0 votes
5 replies
1.4K views
Used featureCounts to generate a count matrix for sc RNA-seq and it has all 0s.
scrna-seq
updated by GenoMax GenoMax • written by Shay • Shay
0 votes
1 reply
1.5K views
Extract bubbles in pangenome graph
graph pangenome bubble gfa
updated by colindaven colindaven • written by polag01 • polag01
0 votes
0 replies
466 views
Databases with GoF (Gain of Function) annotations.
gof lof gain-of-function
written by _quantum_girl_ • _quantum_girl_
0 votes
2 replies
1.3K views
Problem with downloading genome in SnpEff
snpeff
written by Javier • Javier
0 votes
3 replies
1.8K views
Using GPUs in snakemake
parabricks gpu snakemake slurm
written by raphael.B • raphael.B
0 votes
7 replies
2.9K views
STARsolo ; Reads are NOT mapped to transcriptome
starsolo
updated by GenoMax GenoMax • written by gdfsnkfns • gdfsnkfns
0 votes
0 replies
867 views
Comparison between Bracken outputs
bracken kraken taxonomy
written by SushiRoll • SushiRoll
0 votes
4 replies
1.4K views
Adaptive sampling for whole chromosomes
t2t nanopore adaptivesampling aneuploidy centromeres
written by njornet • njornet
0 votes
0 replies
595 views
DESeq2 LRT divergent DEGs ?
interaction lrt deseq2
written by klervi-lugue • klervi-lugue
3 votes
0 replies
1.0K views
Herald: The Biostar Herald for Tuesday, April 09, 2024
herald
written by Biostar Biostar
0 votes
4 replies
1.5K views
Ubuntu storage space is decreasing automatically
ubuntu storage
updated by colindaven colindaven • written by Bushra Bibi Bushra Bibi
0 votes
2 replies
1.2K views
BLASTP short sequences (<20aa) - interpreting results
blast sequence blastp alignment
written by neish • neish
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