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0 votes
0 replies
520 views
STAR total splices (in Log.final) vs collapsed splice junctions (in SJ.out.tab)
star
written by tnminh89 • tnminh89
0 votes
0 replies
634 views
Filter low express genes in microarray data
microarray
written by Chris • Chris
0 votes
3 replies
2.0K views
absolute path for symbolic links in Snakefile
snakemake
updated by Jesse • Jesse • written by yifangt86 • yifangt86
0 votes
2 replies
1.1K views
Hide positions in alignment with 99% "–" characters to ignore single sequence insertions?
alignment gaps
updated by Jesse • Jesse • written by Broccoli • Broccoli
0 votes
3 replies
3.3K views
Source other conda environments in a nextflow pipeline when nextflow itself is in a conda environment?
hpc conda nextflow
updated by Arup Ghosh Arup Ghosh • written by chaco001 • chaco001
0 votes
3 replies
1.7K views
Help understanding how KEGG Ortholog `K00004 ` has 3 ECs associated with it (EC:1.1.1.4, 1.1.1.-, 1.1.1.303)?
ontology metagenomics database enzymes genomics
updated by Mensur Dlakic Mensur Dlakic • written by O.rka O.rka
0 votes
2 replies
1.5K views
Correct way to compare multiple treaments between RNA-Seq samples using edgeR?
rna-seq edger
written by Guille • Guille
1 vote
1 reply
2.2K views
How to use limma to find differentially expressed genes in response to a continuous variable
limma voom r
written by pairedttest • pairedttest
1 vote
6 replies
4.9K views
DNA methylation preprocessing
swan dna methylation funnorm
updated by Ram Ram • written by SYOSY • SYOSY
0 votes
0 replies
972 views
Correlation between cell type prediction scores and individual gene expression in spatial transcriptomic datasets
single-cell spatial-transcriptomics
written by biocellbio • biocellbio
0 votes
11 replies
3.5K views
Create a new bed file with all pairwise combinations between two other bed files, based on bp distance
snps bed eqtl bedtools
written by J • J
0 votes
6 replies
2.6K views
Heatmap and rna-seq
rna-seq heatmap
updated by dsull dsull • written by qudrat.nii • qudrat.nii
5 votes
11 replies
18K views
How Can We Find The Info For 3'Utr And 5'Utr In Gencode Gtf File?
utr
updated by cmdcolin cmdcolin • written by J.F.Jiang J.F.Jiang
0 votes
2 replies
2.0K views
what is the purpose of indexing the reference genome (Kallisto)
indexing kallisto
updated by dsull dsull • written by Aaliya • Aaliya
3 votes
0 replies
769 views
News: Webinar “Learning Single-cell Atlases” with Dr. Fabian Theis, author of Scanpy on May 8
scanpy generative-ai scrna machine-learning
updated by Ram Ram • written by Claire Watson • Claire Watson
0 votes
2 replies
1.2K views
Rare Disease Variant Pathway Analysis
pathway-analysis
updated by Ram Ram • written by The_PyPanda • The_PyPanda
0 votes
0 replies
791 views
Phasing VCF Files and Analyzing Reads with Multiple Variants
haplotypes vcf phasing
written by HarperReed • HarperReed
1 vote
0 replies
803 views
Simulation of label-free bottom-up proteomics expression dataset
label-free bottom-up lc-ms proteomics
written by KABILAN • KABILAN
0 votes
0 replies
636 views
Running Phylogenetic Analysis With NCBI Genome
population-genetics phylogenetic
updated by Ram Ram • written by SineWave • SineWave
0 votes
0 replies
431 views
RNA-seq: full length gene
rna-seq
updated by Ram Ram • written by Nargis • Nargis
0 votes
7 replies
2.6K views
gvcf joint calling
wes gatk vcf gvcf
written by zihanss • zihanss
0 votes
0 replies
546 views
Haplotype Phased Assembly Contigs to Chromosome Annotations
assembly phased haplotype annotation
written by turcoa1 • turcoa1
0 votes
3 replies
1.8K views
Sequence read length shorter than flow cell specification
illumina ngs sequencing
updated by swbarnes2 swbarnes2 • written by M • M
0 votes
0 replies
668 views
Designing single-stable RNA molecules
structure rna
written by Edna • Edna
0 votes
1 reply
911 views
Normalize scRNAseq data to housekeeping genes to compare several datasets
rna-sequencing housekeeping single-cell normalization
updated by ATpoint ATpoint • written by AaronJaime • AaronJaime
0 votes
2 replies
1.2K views
BLAST using both nucleotides and taxonomic local databases
blast ncbi taxid taxonomy
updated by GenoMax GenoMax • written by Begonia_pavonina • Begonia_pavonina
0 votes
3 replies
3.6K views
why renaming Idents in Seurat object doesn't work?
seurat renameidents r
written by Assa Yeroslaviz Assa Yeroslaviz
1 vote
2 replies
1.3K views
ScRNAseq-How to correctly choose cell type marker genes
cellassign cell-markers
written by Francesco • Francesco
1 vote
7 replies
2.3K views
ScRNA data question
scrna vlnplot samples
updated by bk11 • bk11 • written by starswillfade • starswillfade
0 votes
0 replies
629 views
News: Master Meta-analysis with R (May 13-16) - Online!
meta-analysis statistics r
updated by Ram Ram • written by Physalia-courses Physalia-courses
0 votes
0 replies
653 views
How to calculate correlation coefficient for chipseq?
chipseq bigwigsummary deeptools correlation
written by Emily • Emily
1 vote
0 replies
1.1K views
How to calculate reliable Ka/Ks or dN/dS ratio for genes of interest from VCF file
dnds kaks vcf
written by rohitsatyam102 rohitsatyam102
0 votes
0 replies
835 views
how to read graph_test output of monocle 3
monocle3
written by synat.keam • synat.keam
1 vote
0 replies
903 views
Job: CTO/founding engineer role at Voyant Bio
engineer
written by Assaf • Assaf
0 votes
1 reply
1.6K views
GAPIT p-value significance threshold
gapit p-value gwas
updated by ginellegrenier • ginellegrenier • written by Clayton • Clayton
0 votes
4 replies
1.4K views
Why gatk VariantAnnotator required bam and coverage files
gatk variantannotator
written by QX • QX
0 votes
1 reply
795 views
Finding orthologs between genome error
oma omabrowser
updated by Adrian Altenhoff Adrian Altenhoff • written by hemantcnaik • hemantcnaik
1 vote
0 replies
1.1K views
Why not use iBAQ for calculating differential abundance of proteins?
protein maxquant
written by Aspire Aspire
0 votes
5 replies
1.8K views
Figures are disappeared from html report of SnpEff
html snpeff
written by Bushra Bibi Bushra Bibi
0 votes
4 replies
1.6K views
Highest variable features in single cell data
single-cell
written by carolofharvest • carolofharvest
0 votes
1 reply
859 views
Network Alignment
plugin cytoscape cytomcs
updated by Scooter Scooter • written by SKY • SKY
0 votes
4 replies
3.8K views
How could I generate a gi_taxid_nucl.dmp file similar to the one previously hosted by NCBI?
ncbi metagenomics centrifuge gi_taxid_nucl.dmp taxonomy
updated by ZhangYuanfeng • ZhangYuanfeng • written by Frazier Baker • Frazier Baker
0 votes
3 replies
1.2K views
PCA plot
deseq2 pcaplot
updated by LauferVA LauferVA • written by Aaliya • Aaliya
3 votes
5 replies
2.8K views
Tutorial: how to combine multiple RNAseq count files into a single dataframe in R and unix
unix rnaseq r
updated by Mbofire • Mbofire • written by Ming Tommy Tang Ming Tommy Tang
0 votes
4 replies
3.3K views
Lositan freezing when generating selection table
lositan
updated by evawillms183 • evawillms183 • written by andrepleao • andrepleao
0 votes
0 replies
560 views
Can you give me an example of a small globular protein (PDB) that has disordered alpha-carbons?
protein pdb alpha-carbon
written by 4fzcgueyp5 • 4fzcgueyp5
0 votes
3 replies
1.4K views
clustalo - align milions of short seq, memory issue
memory clustalo
updated by Mensur Dlakic Mensur Dlakic • written by emma.a • emma.a
0 votes
2 replies
1.1K views
Annovar using R package
annovar gnomad r
written by DKA • DKA
0 votes
2 replies
1.1K views
Creating Synthetic Sequences for a ML Model
dna ml
updated by Mensur Dlakic Mensur Dlakic • written by biochugs • biochugs
1 vote
8 replies
2.6K views
PCA plot (Suggestions Needed)
deseq2 pcaplot
updated by swbarnes2 swbarnes2 • written by Aaliya • Aaliya
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