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970 results • Page
1 of 20
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0
votes
0
replies
18
views
How to compute TPM normalized values for TCGA miRNA data?
TCGA
miRNA
TPM
normalization
49 minutes ago by
Ngrin
• 0
0
votes
0
replies
22
views
Applying the metacell2 algorithm using python
single-cell
scanpy
cells
meta
python
1 hour ago by
JACKY
▴ 140
1
vote
6
replies
535
views
anRichment is missing
WGCNA
anRichment
updated 1 hour ago by
GenoMax
142k • written 5 months ago by
michael.flower.14
▴ 180
1
vote
1
reply
115
views
Gene ontology and homologs
gene-ontology
updated 2 hours ago by
geneontologyhelp
▴ 400 • written 1 day ago by
beshka194
• 0
1
vote
2
replies
131
views
error in R code: Error in fit_avlr(...), : wn incorrectly formatted
R
updated 41 minutes ago by
zx8754
11k • written 8 hours ago by
snajafy
• 0
0
votes
0
replies
40
views
Cox Model with interaction item interpretation
Cox
4 hours ago by
Bine
▴ 60
0
votes
0
replies
38
views
What is the proper way to identify the cell types of scRNAseq cluster?
scRNAseq
4 hours ago by
MAPK2
▴ 40
0
votes
0
replies
32
views
how to downlad slices of CRAM fro ICGC ARGO
icgc
samtools
cram
4 hours ago by
Javier
• 0
0
votes
4
replies
273
views
Question regarding WGCNA
WGCNA
Network-construction
updated 2 hours ago by
andres.firrincieli
3.6k • written 2 days ago by
deepak
• 0
0
votes
0
replies
41
views
vdjtools
vdjtools
5 hours ago by
yueli7
▴ 250
0
votes
0
replies
57
views
Herald:
The Biostar Herald for Monday, May 06, 2024
herald
6 hours ago by
Biostar
2.7k
0
votes
1
reply
70
views
16S rRNA microbiome differential abundance analysis with DESeq2 finds same microbial Genus in both groups.
microbiome
16S
DESeq2
updated 2 hours ago by
andres.firrincieli
3.6k • written 7 hours ago by
Antonio
• 0
0
votes
0
replies
54
views
Error while loading model of Spatial data deconvolution using cell2location
Python
cell2location
updated 7 hours ago by
Ram
43k • written 7 hours ago by
sidrah.maryam
▴ 50
0
votes
0
replies
44
views
Normalizing AMR gene count data
abundance
RGI
updated 7 hours ago by
Ram
43k • written 8 hours ago by
arshad1292
▴ 100
0
votes
0
replies
63
views
News:
We created a Bioinformatics Competition for Spatial Transcriptomics: MICOS-EU
cell-clustering
Spatial-transcriptomics
updated 7 hours ago by
Ram
43k • written 9 hours ago by
MICOS
• 0
0
votes
0
replies
51
views
Help with VG Toolkit: VCF Output is Empty
vg
updated 10 hours ago by
Pierre Lindenbaum
161k • written 10 hours ago by
sarumonsus
• 0
0
votes
2
replies
231
views
Tissue-specific DEG analysis with DEseq2
DEseq2
RNA-seq
DEG
R
39 minutes ago by
M.
▴ 30
0
votes
0
replies
68
views
How to implement an IGV to streamlit app?
python
streamlit
igv
11 hours ago by
avigail.shnaider
• 0
1
vote
5
replies
239
views
install.packages("imudata") - package ‘imudata’ is not available for this version of R
r
updated 7 hours ago by
Ram
43k • written 13 hours ago by
snajafy
• 0
0
votes
2
replies
161
views
Error in checkFullRank(modelMatrix) : the model matrix is not full rank, so the model cannot be fit as specified.
DESeq2
updated 7 hours ago by
GenoMax
142k • written 1 day ago by
mropri
▴ 150
0
votes
1
reply
260
views
DMRcate ranges liftover hg19 to hg38
DMRcate
Liftover
DMR
Methylation
annotation
updated 19 hours ago by
aaron.stevens
• 0 • written 12 weeks ago by
sativus
▴ 20
2
votes
2
replies
182
views
STRING-DB API can't find my protein but I can find it when I go to the STRING-DB website directly
STRING-DB
protein
STRING-DB-API
21 hours ago by
brandon
• 0
0
votes
0
replies
82
views
Mitogenomes gap closure and making circular genome
gapclosure
Mitogenomes
validation
circulargenome
1 day ago by
KHURRAM SHAHZAD
• 0
1
vote
5
replies
305
views
install package: package ‘gmwm’ is not available for this version of R
r
updated 6 hours ago by
Ram
43k • written 1 day ago by
snajafy
• 0
0
votes
0
replies
83
views
Looking for phytopathogenic, Nitrogen-Fixing, and Growth-Promoting databases for Metagenomic Analysis
Bacteria
Metagenomics
Database
1 day ago by
Christopher
• 0
4
votes
1
reply
190
views
LD-prune variants while maintaining a list of SNPs
snp
vcf
plink
filtering
independent
updated 11 hours ago by
zx8754
11k • written 1 day ago by
Jautis
▴ 560
2
votes
2
replies
275
views
Association study using SNP data
Association
Allele
Parentage
SNP
1 day ago by
drajangirija
• 0
0
votes
0
replies
77
views
News:
Online course: Bioinformatic analysis of transposable elements
GenomeAssembly
Transcriptomics
TransposableElements
ManualCuration
1 day ago by
carlopecoraro2
★ 2.5k
0
votes
0
replies
90
views
Clarification on StringTie's `--merge` parameters
stringtie
stringtie-merge
1 day ago by
DGTool
• 0
0
votes
0
replies
92
views
Seurat V5 integration
Seurat
samples
integration
combine
1 day ago by
starswillfade
▴ 10
0
votes
0
replies
102
views
Problem with Seurat package in PercentageFeatureSet function
cell
single
1 day ago by
Yoosef
▴ 60
0
votes
1
reply
336
views
Job:
Seeking Bioinformatics/Drug Design Opportunities - PhD in Pharmacology with Experience in RNA-seq, CADD & Deep Learning Molecular Generation
search
job
updated 1 day ago by
GenoMax
142k • written 1 day ago by
tulip
• 0
3
votes
4
replies
358
views
Design matrix Differential expression analysis
RNA-seq
Differential-expression
6 hours ago by
SHN
▴ 40
4
votes
2
replies
219
views
GRCh38.gmap file
gnomix
gmap
1 day ago by
lorena9132
▴ 10
2
votes
3
replies
787
views
Issues with Mixture file when using CIBERSORTx
Deconvolution
CIBERSORTx
updated 2 days ago by
vjanve
• 0 • written 7 months ago by
mateomejias
• 0
0
votes
1
reply
229
views
creating batch colum for batch correction
batch-correction
combat
9 hours ago by
Expert
▴ 10
1
vote
4
replies
313
views
Segmentation error while using a tool
smalt
updated 2 days ago by
Mensur Dlakic
★ 27k • written 2 days ago by
Ruqaiya
• 0
0
votes
1
reply
178
views
Help with DGEList function
DGEList
R
updated 22 hours ago by
marco.barr
▴ 90 • written 2 days ago by
Natali
• 0
0
votes
0
replies
124
views
Homer motif analysis
Homer
2 days ago by
daffodil
▴ 10
0
votes
1
reply
164
views
HCL database download
database
HCL
human
updated 2 days ago by
GenoMax
142k • written 2 days ago by
sooni
▴ 20
1
vote
2
replies
1.1k
views
News:
The GDC Legacy Archive is retiring soon.
genomic-data-commons
tcga
gdc
1 day ago by
Zhenyu Zhang
★ 1.2k
2
votes
1
reply
276
views
Force PLINK2 to neglect chrX as the sex chromosome
PLINK2
updated 2 days ago by
chrchang523
10k • written 6 days ago by
8armed
▴ 10
0
votes
5
replies
322
views
Easy way to find out which allele is minor allele from bed file?
plink
updated 2 days ago by
chrchang523
10k • written 3 days ago by
curious
▴ 750
0
votes
1
reply
195
views
visualize GSEA
barplot
RNA-seq
GSEA
enrichment
updated 2 days ago by
Ram
43k • written 3 days ago by
Rob
▴ 170
0
votes
1
reply
189
views
fastq screen aligner no specified.
BWA
Bowtie2
FastqScreen
updated 2 days ago by
GenoMax
142k • written 2 days ago by
Ximena
• 0
0
votes
1
reply
184
views
filtering before dada2
bbduk
qiime2
dada2
metagenomic
updated 2 days ago by
Ram
43k • written 2 days ago by
Valentina
• 0
0
votes
6
replies
395
views
Differential Expression Analysis using DESeq2 in R
deseq2
R
updated 2 days ago by
swbarnes2
14k • written 3 days ago by
Erina
• 0
0
votes
2
replies
211
views
Clarification regarding SAM flags "mate reverse strand" (flag 16/0x10) and "read reverse strand" (flag 32/0x20)
PE
BAM
SAM
flag
paired-end
4 hours ago by
kalavattam
▴ 190
1
vote
3
replies
265
views
TaxID mapping file
annotation
blobtools
RNAseq
decontamination
transcriptomes
1 day ago by
Lada
▴ 30
1
vote
0
replies
142
views
.lgen file has 3+ different alleles
PLINK
lgen
ped
updated 11 hours ago by
zx8754
11k • written 3 days ago by
Emilie
▴ 10
970 results • Page
1 of 20
Recent Votes
Answer: How to get proteins from GFF file resulted from MAKER annotation
Answer: Gene ontology and homologs
Comment: anRichment is missing
Comment: Gene Specific coverage from WGS data
Comment: Gene Specific coverage from WGS data
Comment: High Malat-1 expression in single cell data
A: Meme-Tools: Bit score
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★ 2.7k
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LChart
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▴ 140
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Recent Replies
Comment: Spike-in control found in raw reads (16S amplicon seq) but not picked up by DADA
by
sovrappensiero
▴ 90
Ah, yes this makes sense! I knew this was an issue with, say, using kraken2 on shotgun metagenomic reads, but I don't have a lot of experi…
Comment: How to access TCGA samples that were treated with a specific drug?
by
Qroid
▴ 40
Thank you. Maybe it's best to confirm with the authors about how they're getting responder/non-responder labels. I'll do that now.
Comment: Spike-in control found in raw reads (16S amplicon seq) but not picked up by DADA
by
Chris Dean
▴ 390
If you sequenced a short hypervariable region, most of your sequence reads will not be able to be assigned to the species level. One possi…
Comment: anRichment is missing
by
GenoMax
142k
Consider emailing Dr. Horvath about this. Until enough people make him aware of this issue WGCNA "problem" is unlikely to get fixed.
Comment: anRichment is missing
by
Tatyana
• 0
Unfortunately, there are only tutorials and codes for tutorials for WGCNA, not installation files for any their packages... :(
Comment: anRichment is missing
by
GenoMax
142k
See this link for the WGCNA documentation and code from Peter Langfelder : https://bioinformatics.stackexchange.com/questions/21885/where-t…
Comment: anRichment is missing
by
Tatyana
• 0
The problem is back, Horwath's lab web is down again. Is it possible to get anRichment istallation file somewhere? (The greater problem is …
Comment: 16S rRNA microbiome differential abundance analysis with DESeq2 finds same micro
by
andres.firrincieli
3.6k
1. Maribaculaceae is a family not a genus 2. Who said that ASV with the same taxonomic classification must have the same behaviour? 3. With…
Comment: Question regarding WGCNA
by
andres.firrincieli
3.6k
the within-group variability is so small that these look like technical replicates rather than independent biological replicates
Answer: Gene ontology and homologs
by
geneontologyhelp
▴ 400
No. Homology is defined as shared common ancestry. Two genes that take part in the same biological process may not have the same functio…
Answer: error in R code
by
Matthias Zepper
4.6k
I quickly googled [the source code of that function][1], and the error is triggered by this condition: if(length(wn) < 1 || !is.whol…
Comment: Clarification regarding SAM flags "mate reverse strand" (flag 16/0x10) and "read
by
kalavattam
▴ 190
Thanks for the explanation. I noticed and corrected a typo in which "*read* reverse strand" initially read as "*mate* reverse strand" in t…
Comment: Spike-in control found in raw reads (16S amplicon seq) but not picked up by DADA
by
sovrappensiero
▴ 90
It's a commercial product, a *Staphylococcus hominis* genome. This species is not found in the DADA2 results, so I guess DADA2 is not ident…
Comment: Question regarding WGCNA
by
Ram
43k
Plots are incomplete without legends. Please share the complete plot with mappings for shape and color.
Comment: Quetion regarding WGCNA
by
deepak
• 0
Thank you for your reply andres.firrincieli. I have attached the PCA plot of the expression data used in the WGCNA. ![enter imag…
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