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705 results • Page
1 of 15
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1
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1
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20
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Multiplexing for pooled CRISPR screen sequencing
Illumina
multiplex
CRISPR-screen
updated 18 minutes ago by
Ram
44k • written 45 minutes ago by
Tuấn Anh
• 0
0
votes
1
reply
19
views
Allele count of 2 for homoplasmic MT variants in VCF
Variant-Calling
GATK
VCF
updated 11 minutes ago by
Ram
44k • written 57 minutes ago by
Arton
▴ 10
2
votes
3
replies
1.1k
views
Is it possible to run CibersortX with a very small number of samples?
cibersortx
updated 1 hour ago by
finch
• 0 • written 19 months ago by
Aspire
▴ 330
0
votes
2
replies
172
views
Functional enrichment analysis for unique gene IDs
RNA-SEQ
updated 2 hours ago by
geneontologyhelp
▴ 400 • written 1 day ago by
Pegasus
▴ 100
0
votes
2
replies
83
views
Comparing Analysis Strategies for scRNA-seq Data: Separate vs. Merged Analysis of Spleen Samples from Different Conditions
Seurat
scRNA-Seq
Single-Cell
2 hours ago by
Shukai
• 0
0
votes
1
reply
49
views
Question about samtools view flags (paired reads vs. properly paired reads)
samtools
updated 3 hours ago by
Pierre Lindenbaum
161k • written 3 hours ago by
mrk
• 0
0
votes
2
replies
106
views
z-score of gene set
z-score
scRNA
gene-set
updated 2 hours ago by
Ram
44k • written 5 hours ago by
Hien
• 0
2
votes
1
reply
79
views
converting transcript IDs (Ensembl) to gene symbols in R
RNAseq
updated 4 hours ago by
bk11
★ 2.5k • written 5 hours ago by
ashkan
▴ 160
0
votes
0
replies
39
views
Linking AMR Genes to Taxonomy in Soil Metagenomics
metagenomics
bacteria
wgs
AMR
resistome
updated 4 hours ago by
GenoMax
142k • written 4 hours ago by
LuciaNhu
• 0
1
vote
2
replies
81
views
How can we convert a vcf to fasta, so that I can blast some genes against that whole genome sequences?
blast
fasta
vcf
gene
updated 1 hour ago by
Ram
44k • written 4 hours ago by
Harshita
• 0
2
votes
0
replies
46
views
Comparing ATAC-seq narrowPeak Files from Chicken and Mouse
liftOver
ATAC-seq
csaw
updated 19 minutes ago by
Ram
44k • written 4 hours ago by
Rafael Soler
★ 1.2k
0
votes
0
replies
49
views
Job:
PhD position for metagenomics analysis at the University of Udine
metagenomics
updated 20 minutes ago by
Ram
44k • written 4 hours ago by
Fabio Marroni
★ 3.0k
0
votes
0
replies
46
views
How does Kraken2 deal with poor read qualities?
kraken
qiime2
kraken2
qiime
updated 4 hours ago by
GenoMax
142k • written 5 hours ago by
Christopher
• 0
3
votes
5
replies
410
views
Practical Haplotype Graph v2 not finding correct paths
Pangenome
PHG
graph
5 hours ago by
beantkapoor16
▴ 10
0
votes
1
reply
73
views
DRAGEN gVCF files and joint calling GATK-GenomicsDB
DRAGEN
GenomicsDB
updated 5 hours ago by
DBScan
▴ 300 • written 6 hours ago by
brunomiwa
• 0
0
votes
0
replies
47
views
Last step of metagenome analysis before visualization
metagenome
python
analyis
6 hours ago by
Ayda Ecem
• 0
0
votes
3
replies
104
views
Generating a Bed file from a Fasta file
bed
NullSeq
updated 18 minutes ago by
Ram
44k • written 6 hours ago by
pirku
• 0
0
votes
3
replies
1.7k
views
Running STRUCTURE from command line
STRUCTURE
updated 10 minutes ago by
Ram
44k • written 3.0 years ago by
giulia.trauzzi
▴ 10
1
vote
3
replies
520
views
Pruning Phylogenetic Trees and Bootstrap Values
phylogenetics
bootstrap
updated 8 hours ago by
Klaus S
▴ 160 • written 5 weeks ago by
Zeng Hao
▴ 40
0
votes
0
replies
52
views
Calculated LAI is too large
annotation
LAI
assembly
TE
genome
9 hours ago by
Yao
▴ 30
1
vote
1
reply
696
views
Herald:
The Biostar Herald for Monday, November 01, 2021
herald
written 2.6 years ago by
Biostar
2.8k
17
votes
10
replies
7.3k
views
7 follow
T2T-CHM13 "complete" human genome gff/gtf annotation file
genomics
written 3.0 years ago by
noodle
▴ 580
0
votes
0
replies
58
views
How to recover the entire sequence from all_contig_annotations.csv?
VDJ
single-cell
TCR-seq
cellranger
10x
11 hours ago by
dawnyipingzou
• 0
0
votes
4
replies
151
views
Where to find old version of GATK best practice
gatk
2 hours ago by
Zhenyu Zhang
★ 1.2k
0
votes
1
reply
85
views
Nextflow docker: Error response from daemon
sarek
gatk4
docker
nextflow
updated 12 hours ago by
Pierre Lindenbaum
161k • written 12 hours ago by
Peter Chung
▴ 200
0
votes
0
replies
57
views
News:
Online course- Advanced Python for Life Sciences
Python-Programming
Data-Visualisation
Machine-Learning
updated 10 minutes ago by
Ram
44k • written 13 hours ago by
carlopecoraro2
★ 2.5k
0
votes
1
reply
97
views
CreateSeuratObject taking very long
seurat
updated 7 hours ago by
Nitin Narwade
★ 1.6k • written 16 hours ago by
eae6d2e7
• 0
0
votes
0
replies
65
views
Multi-ploid data in ANGSD and NGSadmix
ancestry
diploid
haploid
admixture
17 hours ago by
Christopher
• 0
1
vote
2
replies
168
views
Elbow plot question (scRNA seq data analysis - scanpy tutorial)
scanpy
22 hours ago by
ojaswinipandey
• 0
0
votes
2
replies
160
views
Kraken2 Custom Database non-deterministic results
Metagenomics
kraken2
5 hours ago by
Bjorn
• 0
1
vote
4
replies
262
views
How to find SRA sequences of some fungal whole genome sequences if only Biosample ID is available?
SRA
whole-genome-sequencing
NCBI
updated 23 hours ago by
Ram
44k • written 1 day ago by
Harshita
• 0
2
votes
4
replies
203
views
Filter Genome for Specific Sites
bedtools
updated 23 hours ago by
Ram
44k • written 1 day ago by
Anita
• 0
0
votes
3
replies
218
views
Error in using BioSampleParser tool: subscript out of bounds
R
updated 22 hours ago by
marco.barr
▴ 130 • written 1 day ago by
Mohamed Samir
▴ 20
1
vote
1
reply
99
views
Generating Group/Cluster Lists from fastq files
Sequencing
RNA-seq
DNA-Seq
updated 1 day ago by
GenoMax
142k • written 1 day ago by
niruf
• 0
0
votes
0
replies
74
views
News:
Online course: Network analysis in Systems Biology with R/Bioconductor
Bioconductor
Systems-Biology
Network-Analysis
R
updated 23 hours ago by
Ram
44k • written 1 day ago by
carlopecoraro2
★ 2.5k
2
votes
0
replies
106
views
Herald:
The Biostar Herald for Monday, May 20, 2024
herald
1 day ago by
Biostar
2.8k
2
votes
5
replies
374
views
Super ehancers
enhancers
updated 30 minutes ago by
jared.andrews07
★ 17k • written 19 days ago by
Oburah
• 0
0
votes
1
reply
112
views
vcf phasing
beagle
WhatsHap
phasing
updated 7 hours ago by
WouterDeCoster
47k • written 1 day ago by
safeassli
▴ 10
0
votes
2
replies
185
views
How to generate an Upset plot in R to plot the shared variants between cell free DNA samples
r
Upset
1 day ago by
sainavyav22
• 0
0
votes
1
reply
595
views
Illumina methylation EPIC V2 array
ewas
methylation
enrichment
missMethyl
EPIC
updated 1 day ago by
fu_entomology
▴ 40 • written 8 months ago by
juliviglino
• 0
1
vote
2
replies
169
views
How to find identical sequences in genome fasta file (by Python or any possible program) ?
fasta
updated 1 day ago by
Ram
44k • written 1 day ago by
Sony
▴ 10
0
votes
3
replies
180
views
GATK Structural Variants Pipeline - Steps
SV
GATK
Variant-Calling
updated 1 day ago by
Ram
44k • written 1 day ago by
Bioinformatics_begginner
▴ 20
0
votes
0
replies
84
views
Is it possible to keep gene_id and gene_name columns using Seurat::Read10X?
Seurat
single-cell
scRNA-seq
updated 1 day ago by
Ram
44k • written 1 day ago by
ev97
▴ 20
0
votes
1
reply
130
views
GO analysis: Indicate genes corresponding to the pathway
GeneOntoloy
GO
R
updated 1 day ago by
marco.barr
▴ 130 • written 1 day ago by
sooni
▴ 20
0
votes
1
reply
98
views
How to hide label in layout file of MCscan (python version)
MCscan
updated 1 day ago by
GenoMax
142k • written 1 day ago by
jinyi
• 0
0
votes
0
replies
75
views
ENCORI or starBase : ceRNA data retrieval using Web API and curl
ceRNA
ENCORI
curl
microRNA
starBase
1 day ago by
V_Vibes
• 0
0
votes
0
replies
144
views
How to deal with possibly important predictors omitted during the building of an OLS multivariant linear regression model?
multivariant-linear-regression
updated 23 hours ago by
Ram
44k • written 1 day ago by
JorgeVallejo
▴ 20
0
votes
0
replies
96
views
transcriptome annotation
annotation
trinity
transcriptome
updated 1 day ago by
Ram
44k • written 1 day ago by
Asim Bin Arshad
• 0
0
votes
5
replies
222
views
Why most genes have high padj values
RNA-seq
DEG
updated 1 day ago by
Ram
44k • written 1 day ago by
mnx0723
• 0
7
votes
14
replies
2.9k
views
6 follow
How to find tandem duplications pattern in a DNA sequence
Repeat
updated 1 day ago by
micah
▴ 30 • written 3.1 years ago by
kumajis
• 0
705 results • Page
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Recent Votes
Comment: Multiplexing for pooled CRISPR screen sequencing
Comment: How can we convert a vcf to fasta, so that I can blast some genes against that w
Answer: Differential Expression with limma: Contrast and Design Matrix, combinatorial ap
Answer: tensorQTL interaction issue
Answer: converting transcript IDs (Ensembl) to gene symbols in R
Answer: converting transcript IDs (Ensembl) to gene symbols in R
Comment: Trimming tool
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Recent Replies
Comment: Allele count of 2 for homoplasmic MT variants in VCF
by
Ram
44k
Please stop using `bioinformatics` as a tag unless your post is about the field of bioinformatics itself.
Comment: Multiplexing for pooled CRISPR screen sequencing
by
GenoMax
142k
> If we switch to pair-end sequencing with dual-index barcode You can use dual-indexing with single end reads. You don't need to do paired…
Comment: Super ehancers
by
jared.andrews07
★ 17k
That is not valid GFF format, so that is probably going to be problematic. See the [GFF spec](https://github.com/The-Sequence-Ontology/Spec…
Comment: Error in CIBERSORTx ($ operator is invalid for atomic vectors)
by
finch
• 0
Hey! I had this issue and I think I can help- could you take a screenshot of the .txt file opened in the notepad?
Answer: Is it possible to run CibersortX with a very small number of samples?
by
finch
• 0
Hi! I had a very similar issue and it's likely not at all related to your number of samples but to the format of your mixture file! Ciberso…
Comment: How can we convert a vcf to fasta, so that I can blast some genes against that w
by
Ram
44k
You cannot *convert* a VCF to a FASTA as they have vastly different information content. Please explain your process - what exactly do you …
Comment: Comparing Analysis Strategies for scRNA-seq Data: Separate vs. Merged Analysis o
by
Shukai
• 0
Thank you Ram for the comments! Noted for future posts.
Comment: Where to find old version of GATK best practice
by
Zhenyu Zhang
★ 1.2k
I am looking for diagrams, and step-by-step instructions of how to build my pipelines. I think the github is only helpful if you are a wdl…
Comment: Where to find old version of GATK best practice
by
Zhenyu Zhang
★ 1.2k
oh, this is helpful. Thanks.
Comment: Comparing Analysis Strategies for scRNA-seq Data: Separate vs. Merged Analysis o
by
Ram
44k
1. You can edit your post and add relevant details. There is no need to add separate comments with relevant details especially when it's no…
Answer: Functional enrichment analysis for unique gene IDs
by
geneontologyhelp
▴ 400
If you are looking at producing GO annotations, we currently only recommend InterProScan, and basically only for novel organisms or where t…
Answer: Question about samtools view flags (paired reads vs. properly paired reads)
by
Pierre Lindenbaum
161k
paired reads = the experiment was a paired end assay: each fragment was sequenced on 5' and 3', two FASTQ files are generated by the seque…
Answer: z-score of gene set
by
Matthias Zepper
4.6k
The z-score is a measure of how many standard deviations a particular data point is from the mean of a distribution. A positive z-score ind…
Answer: converting transcript IDs (Ensembl) to gene symbols in R
by
bk11
★ 2.5k
You can do as following: library(biomaRt) # Set up the Ensembl BioMart connection ensembl <- useEnsembl(biomart = "genes",…
Comment: How can we convert a vcf to fasta, so that I can blast some genes against that w
by
Pierre Lindenbaum
161k
well you try to use FastaAlternateReferenceMaker https://gatk.broadinstitute.org/hc/en-us/articles/360037594571-FastaAlternateReferenceMak…
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