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116,746 results • Page
1 of 2335
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Votes
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0
votes
0
replies
4
views
visualize GSEA
enrichment
barplot
RNA-seq
GSEA
positive
7 minutes ago by
Rob
▴ 170
0
votes
5
replies
156
views
Differential Expression Analysis using DESeq2 in R
deseq2
R
19 minutes ago by
Erina
• 0
0
votes
1
reply
16
views
Clarification regarding SAM flags "mate reverse strand" (flag 16/0x10) and "read reverse strand" (flag 32/0x20)
PE
BAM
SAM
flag
paired-end
updated 20 minutes ago by
swbarnes2
14k • written 31 minutes ago by
kalavattam
▴ 190
0
votes
1
reply
30
views
TaxID mapping file
annotation
blobtools
RNAseq
decontamination
transcriptomes
updated 1 hour ago by
GenoMax
142k • written 2 hours ago by
Lada
▴ 30
0
votes
0
replies
32
views
.lgen file has 3+ different alleles
PLINK
lgen
ped
3 hours ago by
Emilie
• 0
0
votes
4
replies
94
views
Easy way to find out which allele is minor allele from bed file?
plink
1 hour ago by
curious
▴ 750
0
votes
0
replies
24
views
vcf procesing for ML antigen prediction
formats
vcf
fastaq
HLA_imputation
HLA_typing
3 hours ago by
Javier
• 0
0
votes
0
replies
25
views
Extracting mutation status from MAF files - TCGA
TCGA
maf
mutation
4 hours ago by
yahn
• 0
795
votes
167
replies
142k
views
109 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 3 months ago by
Biostar
2.7k • written 7.4 years ago by
Istvan Albert
100k
0
votes
0
replies
36
views
IGV interpretation for ATM gene for chromosome 11 for a deceased partient suspected of having melanoma
genetic
Variant
predisposition
4 hours ago by
mikemakaveli1
• 0
0
votes
0
replies
35
views
Tissue-specific DEG analysis with DEseq2
DEseq2
RNA-seq
DEG
R
updated 4 hours ago by
Ram
43k • written 4 hours ago by
M.
▴ 30
0
votes
2
replies
82
views
Raw counts using stringtie
stringtie
RNA-seq
updated 4 hours ago by
GenoMax
142k • written 5 hours ago by
ahmad.sajad4541
• 0
0
votes
0
replies
34
views
Modify plot R mtDNA indel
plot
mtDNA
R
6 hours ago by
marco.barr
▴ 90
0
votes
0
replies
37
views
Re-Import modified clusterProfiler's GSEA result from .csv for graphical output-generation (i.e. cnetplot, heatplot)
enrichplot
graphical_output
GSEA
clusterProfiler
6 hours ago by
NorbertK
• 0
2
votes
2
replies
114
views
Analysis of intronic reads included scRNA-seq data
cell
single
updated 6 hours ago by
t.montserrat.ayuso
▴ 10 • written 10 hours ago by
carolofharvest
▴ 30
9
votes
9
replies
424
views
High Malat-1 expression in single cell data
cell
analysis
single
updated 4 hours ago by
dsull
★ 6.0k • written 4 days ago by
carolofharvest
▴ 30
0
votes
1
reply
82
views
Downloading older version of a tool
Alignment
tools
updated 7 hours ago by
GenoMax
142k • written 8 hours ago by
Ruqaiya
• 0
0
votes
0
replies
51
views
Generating illumina interops SAV like plots for multiqc reports on HPC
visualization
illumina
ngs
sequencing
8 hours ago by
rustykb
▴ 20
1
vote
1
reply
74
views
DSEQ2 analysis
DSEQ2
logfoldchane
updated 10 hours ago by
ATpoint
82k • written 10 hours ago by
adi.gershon1
• 0
3
votes
5
replies
167
views
Generating mpileup file using samtools
mpileup
samtools
updated 7 hours ago by
Joe
21k • written 10 hours ago by
Ruqaiya
• 0
0
votes
5
replies
230
views
6 follow
What purposes can TPM values be used for?
RNA-seq
TPM
Normalization
DESeq2
updated 11 hours ago by
b.contreras.moreira
▴ 180 • written 1 day ago by
JH
• 0
1
vote
3
replies
185
views
Does comparing two different groups to a common third group introduce bias in the analysis?
DESEQ
RNAseq
updated 12 hours ago by
ATpoint
82k • written 22 hours ago by
Qi
• 0
0
votes
0
replies
53
views
News:
Cellosaurus release 49 is available
cell
biocuration
line
database
news
knowledgebase
13 hours ago by
Amos Bairoch
▴ 120
1
vote
3
replies
159
views
Help with choosing a model species for Augustus for a de novo assembled genome.
augustus
annotation
assembly
genome
updated 4 hours ago by
dthorbur
★ 1.9k • written 13 hours ago by
Vijith
▴ 30
0
votes
0
replies
72
views
SIngle cell analysis
Seurat
scRNA-seq
Harmony
DoubletFinder
Merge
14 hours ago by
Jeyong
• 0
0
votes
2
replies
659
views
homer not configured properly
Homer
makeTagDirectory
updated 16 hours ago by
clairechung112
• 0 • written 23 months ago by
amahdi779
• 0
1
vote
3
replies
173
views
Bedtools merge minimum overlap?
bedtools
genomics
updated 17 hours ago by
Alex Reynolds
35k • written 1 day ago by
SJP
• 0
0
votes
0
replies
69
views
Empty table plot using plotGseaTable()
FGSEA
22 hours ago by
Chris
▴ 260
0
votes
1
reply
115
views
VG : No reference-sense paths available in the graph; falling back to generic paths.
vg
updated 23 hours ago by
anovak
▴ 120 • written 1 day ago by
Hang
• 0
1
vote
3
replies
163
views
DiffBind: no peaks in DBA
MACS2
DiffBind
ChIP-seq
updated 23 hours ago by
jared.andrews07
★ 16k • written 1 day ago by
yvonneh
▴ 10
3
votes
5
replies
174
views
Converting CRAM to FastQ
GATK
samtools
cram
SamToFastq
fastq
1 hour ago by
Maverick
▴ 10
3
votes
8
replies
6.5k
views
7 follow
GATK genomicsDBimport intervals for WGS
gatk
genomicsdbimport
updated 1 day ago by
Sd
• 0 • written 5.1 years ago by
Nicolas Rosewick
11k
0
votes
2
replies
127
views
Base Quality Score Recalibration with different reference genome
SNPs
GATK
BQSR
RNA-seq
1 day ago by
dtnondorf
• 0
0
votes
1
reply
154
views
Spike-in control found in raw reads (16S amplicon seq) but not picked up by DADA2 - where to go from here?
nf-core
16S
amplicon
dada2
ampliseq
updated 17 hours ago by
Chris Dean
▴ 390 • written 1 day ago by
sovrappensiero
▴ 90
1
vote
2
replies
177
views
RNA-seq data for deep learning classification
rna-seq
7 hours ago by
yahn
• 0
2
votes
6
replies
277
views
How to access TCGA samples that were treated with a specific drug?
TCGA
RNA-seq
19 hours ago by
Qroid
▴ 40
0
votes
1
reply
473
views
Loss of 'var' using concatenation of AnnData objects
Scanpy
AnnData
updated 1 day ago by
Hugo
• 0 • written 4 months ago by
Rachel
• 0
0
votes
1
reply
110
views
Provean help
variant
Provean
updated 1 day ago by
Mensur Dlakic
★ 27k • written 1 day ago by
Arun Sai Kumar
• 0
1
vote
1
reply
127
views
Download eQTL data of one specific gene for all tissues from GTEx
eQTL
GTEx
updated 1 day ago by
bk11
★ 2.4k • written 1 day ago by
Jeol
▴ 20
1
vote
1
reply
136
views
Super ehancers
enhancers
updated 1 day ago by
jared.andrews07
★ 16k • written 1 day ago by
Oburah
• 0
0
votes
6
replies
242
views
How does gene length effect the number of reads mapped
RNA-seq
CPM
sequencing
updated 5 hours ago by
dsull
★ 6.0k • written 1 day ago by
Chen
• 0
5
votes
5
replies
222
views
Problem with data downloaded from Short Reads Archive (SRA)
SRA
DADA2
metabarcoding
updated 1 day ago by
atharvakarkare14
▴ 30 • written 1 day ago by
Begonia_pavonina
▴ 150
1
vote
7
replies
406
views
Downsampling long-read BAM files
nanopore
BAM
QC
ONT
downsampling
8 hours ago by
eebloom
▴ 80
3
votes
3
replies
252
views
What analysis suitable to identify similarly expressed genes between two samples (treated vs untreated). Contrary to DGE analysis,
Gene-Expression
RNA
NOISeq
DGE
updated 10 hours ago by
ATpoint
82k • written 1 day ago by
alifafiq1
• 0
0
votes
0
replies
83
views
Use of annotation of integrated Seurat object in single sample
Seurat
1 day ago by
Bine
▴ 60
0
votes
0
replies
90
views
News:
Beyond beginner R: Advancing your coding skills - September 16-19
Advanced-Coding
Programming
R
updated 1 day ago by
Ram
43k • written 1 day ago by
carlopecoraro2
★ 2.5k
0
votes
1
reply
208
views
Subsetting and merging back Seurat Object brings different results
Seurat
1 day ago by
Bine
▴ 60
0
votes
0
replies
114
views
Job:
Postdoctoral Position in Bioinformatics - Mainz, Germany
NGS
Genomics
Post-doc
R-loops
updated 1 day ago by
Ram
43k • written 1 day ago by
4r-rtg
• 0
1
vote
1
reply
129
views
Discrepancy in read counts from fastq
downsampling
fastq
updated 1 day ago by
Ram
43k • written 1 day ago by
marco.barr
▴ 90
0
votes
0
replies
82
views
edgeR contrasts for analysis of reciprocal hybrid crosses - averaging, pooling, and comparing groups
edgeR
1 day ago by
Rhiannon
• 0
116,746 results • Page
1 of 2335
Recent Votes
how to combine multiple RNAseq count files into a single dataframe in R and unix
Comment: Greatly speed up conda by using mamba
Greatly speed up conda by using mamba
Comment: Converting CRAM to FastQ
Parametric or non-parametric tests for qPCR significance testing between treatments?
Answer: Converting CRAM to FastQ
Answer: Does comparing two different groups to a common third group introduce bias in th
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Rob
▴ 170
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GenoMax
142k
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curious
▴ 750
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dsull
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Answer: Differential Expression Analysis using DESeq2 in R
by
Erina
• 0
![Here is the code I am using for the analysis][1] [1]: /media/images/8a511904-6ae8-47f5-b647-ee15b02b
Answer: Clarification regarding SAM flags "mate reverse strand" (flag 16/0x10) and "read
by
swbarnes2
14k
In paired end sequencing, every read has a mate, and the flags all together tell you not only what is up with the read you are looking at, …
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix
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Comment: Easy way to find out which allele is minor allele from bed file?
by
curious
▴ 750
According to the link: "When *generating* such filesets, PLINK 1.x defaults to swapping the alleles whenever A1's frequency is above (not …
Comment: TaxID mapping file
by
GenoMax
142k
nodesDB file should have been installed if you had used "Install" script for `blobtools` according to : https://blobtools.readme.io/docs/ta…
Comment: Parametric or non-parametric tests for qPCR significance testing between treatme
by
e.r.zakiev
▴ 210
obviously if the values are really sampled from a (quasi) normally-distributed values, then n doesn't matter, he could use t-test even with…
Comment: Converting CRAM to FastQ
by
Maverick
▴ 10
Understood Thank you!
Comment: Converting CRAM to FastQ
by
GenoMax
142k
You can use any reference that you like at this point. Since you are planning to use GATK, you can get their version from the resource bund…
Comment: Converting CRAM to FastQ
by
Maverick
▴ 10
![enter image description here][1]I was able to get my fastq files. I can see that my reference files are cached in a hts-ref directory but…
Comment: Differential Expression Analysis using DESeq2 in R
by
swbarnes2
14k
Okay, that shows us the problem, but not what we need to solve it. But, for instance, are you sure that period after the sample name shou…
Comment: Easy way to find out which allele is minor allele from bed file?
by
bk11
★ 2.4k
In the case of MAF = 0.5, I think A1 is still the minor allele. You can check the discussion in this [link][1]. [1]: https://groups.goo…
Comment: Easy way to find out which allele is minor allele from bed file?
by
curious
▴ 750
Yes, but I think the problem is what to do when MAF = 0.5
Answer: Easy way to find out which allele is minor allele from bed file?
by
bk11
★ 2.4k
In Plink, A1 is usually a minor allele and A2 a major allele. .frq (basic allele frequency report) Produced by --freq. …
Comment: Raw counts using stringtie
by
GenoMax
142k
> Error parsing strand from GFF line Looking at this error and the listing of files in your directory it looks like you need to `gunzip` t…
Comment: High Malat-1 expression in single cell data
by
dsull
★ 6.0k
I briefly looked at those genes on a genome browser -- there are a lot of repeat elements in those genes, meaning a lot of counts assigned …
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