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0 votes
4 replies
1.3K views
How to Annotate Gene names in *.bedmethyl files generated from ModKit?
genomics nanopore multiomics modkit metagenomics
written by Vaishnav 0
0 votes
5 replies
1.3K views
Building Salmon and STAR index using rat GRCr8 reference genome
star index salmon grcr8
written by Ajina 0
0 votes
7 replies
1.7K views
Alternatives to Cellbender / barcode selection tools foor poor quality single NUCLEI samples?
nuclei single scrnaseq cellbender
updated by Bastien Hervé 680 • | written by txema.heredia 31
0 votes
1 reply
1.6K views
Getting intensities using apt-genotype-axiom
genotype assembly pmra affymetrix
updated by s.w.vanderlaan 5 • | written by Genotepes 95
0 votes
2 replies
1.2K views
News: Prefer in-person over online? 2026 NGS workshops in Leipzig, Berlin & Munich (NGS, single-cell, epigenomics)
singlecell rnaseq dnaseq epigenomics nextflow
written by ecSeq Bioinformatics 9
0 votes
1 reply
2.1K views
Different alternatives for downloading GWAS summary statistics en masse
gwas
updated by GrandmaFunk 3 • | written by LauferVA 497
1 vote
3 replies
1.1K views
Job: Bioinformatics Specialist (Infrastructure Focus)
infrastructure nextflow pipelines
written by Madelaine Gogol 536
8 votes
0 replies
560 views
Tool: FastDup: A scalable, high-performance duplicate marking tool (20x faster than Picard with identical results)
marking-duplicate genome-analysis
written by YeWen 18
0 votes
6 replies
1.8K views
Running Purge_dups on Hifiasm assemblies.
genome assemblies purge_dups
updated by shelkmike 201 • | written by SomeOne 33
1 vote
1 reply
988 views
GDCprepare() fails for TCGA-LAML: Error in dplyr::select - Column `disease_response` doesn't exist
tcgabiolinks bioconductor tcga r
updated by shahab 0 • | written by amirnavidinia2014 2
0 votes
5 replies
1.2K views
Expected behavior of Salmon with --numGibbsSamples 100?
bootstrap gibbs sampling salmon edger
updated by Gordon Smyth 916 • | written by Tonya S. 1
0 votes
2 replies
683 views
Separating stubborn fibroblasts from epithelial cells in primary lung cancer cultures
primary-cell-culture epithelial-cells cell-separation fibroblast
updated by ATpoint 9.1K • | written by Havva 0
0 votes
0 replies
579 views
News: Assembly and Annotation of Genomes course - online, 9-13 March
pacbio genomeassembly annotation nanopore
written by Physalia-courses 281
1 vote
1 reply
982 views
Forum: A Call for Transparency in Citation Practices in the 2018 Galaxy Update (doi: 10.1093/nar/gky379)
citation self galaxy bioinformatics
updated by ATpoint 9.1K • | written by Free 1
0 votes
4 replies
1.4K views
RNAseq results from HEK293T cells can or cannot be used for Central Nervous System?
biology rnaseq transcriptomics
written by R.L. 0
1 vote
4 replies
1.3K views
Building a custom genomic database
annotation database genome
updated by 5heikki 1.1K • | written by anikcropscience 28
2 votes
8 replies
1.7K views
Forum: Interest in an on-premise automated pipeline for proprietary vendor sequencing data (e.g. Illumina)?
dragen illumina automation pipeline
updated by Jeremy Leipzig 2.3K • | written by Matvii Mykhailichenko 2
0 votes
5 replies
1.5K views
Should use Ambient RNA detection software for filtered_bc_matrices from Cell Ranger for snRNAseq
ambient snrnaseq 10x cellranger
updated by carolofharvest 6 • | written by biotrekker 11
0 votes
0 replies
508 views
Job: Two internships in Nantes/France (In French)
nantes france internships
written by Pierre Lindenbaum 17K
1 vote
2 replies
605 views
Nextflow process fails with “Invalid method invocation call” despite correct tuple
nextflow frip
written by DdogBoss 7
0 votes
2 replies
724 views
PASA- Structural annotation of genome assembly
pasa
updated by colindaven 844 • | written by rachana 0
0 votes
1 reply
536 views
Is there any way to download the table of predicted targets from miRDB?
mirdb
updated by GenoMax 16K • | written by jhonatan.henrique 4
0 votes
0 replies
391 views
Statistical test to look for peak differentiation between two manhattan plots
statistics glm popgen r manhattan
written by jfaberha 5
4 votes
2 replies
1.0K views
Herald: The Biostar Herald for Tuesday, November 11, 2025
herald
updated by GenoMax 16K • | written by Biostar 380
0 votes
1 reply
715 views
Tutorial: AI tutor for iCn3D to provide step-by-step instructions on how to view and analyze 3D structures
tutorial structure icn3d ai
written by Jiyao Wang 38
0 votes
1 reply
483 views
What does PolyPhen-2 error mean?
polyphen-2
updated by Ram 4.5K • | written by Jl 1
0 votes
2 replies
1.3K views
ChIPSeq Analysis on GEO Fastq data on Galaxy
chip-seq geo
updated by gynecoloji 0 • | written by mjdm 0
0 votes
4 replies
938 views
scRNAseq annotation
annotation immune scrnaseq tumor
updated by bioadam 1 • | written by mrashad 9
0 votes
1 reply
841 views
Trouble getting clean alignment and searching for sequences of interest
sequence blast phylogenetics alignment protein
updated by GenoMax 16K • | written by rodeorogue 0
0 votes
0 replies
715 views
WGCNA - module genes change with different input order of genes in expression data, but all other values are identical
co-expression analysis wgcna network
written by soy-cat 0
0 votes
0 replies
735 views
News: Online Course: Machine Learning for Multi-Omics Integration
data-integration bigdata multi-omics depp-learning machine-learning
written by Physalia-courses 281
0 votes
0 replies
359 views
Differences in start/end coordinates in mothur
mothur 16s edna
written by mainly.microbe2 0
0 votes
0 replies
378 views
News: Unlock Biological Insights for Bioengineering using QIAGEN IPA
omicsdata pathwayanalysis bioengineeringresearch
updated by GenoMax 16K • | written by Nicole 12
0 votes
8 replies
2.7K views
How do I convert FASTQ files to VCF files?
gatk samtools vcf
written by CaERu _no_ korokoro 0
3 votes
7 replies
1.6K views
Can I integrate two scRNAseq datasets using DE genes?
seurat single cell integration
updated by antonioggsousa 344 • | written by tais.bv 3
4 votes
5 replies
1.5K views
News: Malicious Rust libraries for bioinformatic software: sha-rst et al.
malware rust security
written by Matthias Zepper 524
1 vote
3 replies
1.8K views
RNA-seq: identifying pericentromeric region
genomics transcriptomics rna-seq assembly aligning
updated by William 2 • | written by Polly 1
0 votes
0 replies
252 views
MD of metalloprotein
gromacs md
written by Ria 0
0 votes
10 replies
2.4K views
How to handle a scattered cluster with mixed cell type markers in scRNA-seq subclustering analysis?
clustering scrna-seq findallmarkers
updated by Arup Ghosh 402 • | written by maplewj 2
0 votes
5 replies
1.3K views
scDNAseq data
scdnaseq r variations cnv studio
updated by GenoMax 16K • | written by baldinifrancesca92 0
0 votes
4 replies
1.9K views
How do I choose the correct Trimmomatic parameters based on FastQC results?
trimmomatic quality-control fastqc rna-seq
updated by Istvan Albert 10K • | written by abdulmajeedkhattak1 0
0 votes
4 replies
1.3K views
Can't seem to integrate similar datasets
seurat integration
written by tais.bv 3
0 votes
2 replies
1.2K views
Tool: Subseq - New Protein Design and Analysis Web UI + API service
bindcraft gromacs alphafold rfdiffusion api
written by Emin Bursa 0
0 votes
8 replies
3.3K views
MACS2 “0 reads have been read” error after alignmentSieve in ATAC-seq Nextflow pipeline (single-end)
deeptools nextflow atac-seq macs2
written by DdogBoss 7
0 votes
0 replies
270 views
How retaining multi-mapping reads affects CHiCAGO interaction calls (PCHI-C data)
reads pchi-c multi-mapped chicago
written by Yukino 0
0 votes
0 replies
383 views
News: From manual scripts to reproducible workflows: live online Nextflow course (Jan 19–21, 2026)
programming workflow workshop pipeline_analysis nextflow
written by ecSeq Bioinformatics 9
0 votes
0 replies
422 views
How to run MD simulation for metalloprotein having zinc in GROMACS?
gromacs md
written by Ria 0
0 votes
1 reply
915 views
Forum: Seeking advice: How did CAT aspirants from science backgrounds decide if an MBA was right for them?
carreer advice
updated by Michael Dondrup 5.6K • | written by Prachi 0
0 votes
4 replies
2.1K views
Can i use orthofinder for small protein datasets and not full proteome?
orthofinder effectors
updated by Emin Bursa 0 • | written by alexandrakortsi 0
1 vote
1 reply
1.4K views
vg call AD frequencies for certain sites
vg
updated by tatianabellagio 0 • | written by Danny 1
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