This is a test version of Biostars. For the public version, visit https://www.biostars.org.
0 votes
4 replies
2.1K views
Randomizing train-test data split for classification tasks.
split rnaseq test train
written by Shakunthala Natarajan • Shakunthala Natarajan
0 votes
7 replies
2.0K views
Missing required field on nf-core/sopa
nf-core sopa
updated by Maxime Garcia Maxime Garcia • written by jomagrax • jomagrax
0 votes
0 replies
611 views
News: Course: Flow Cytometry data analysis with R/Bioconductor - online, 2-4 February
r cancer-genomics flow-cytometry bioconductor
written by Physalia-courses Physalia-courses
0 votes
7 replies
8.1K views
Need help in Single cell proteomics data analysis
single cell
updated by Gordon Smyth Gordon Smyth • written by Saber_J • Saber_J
0 votes
5 replies
9.0K views
Best way to do protein structure guided similarity search?
alphafold blast structure proteins
updated by yl759 • yl759 • written by Mark • Mark
1 vote
6 replies
8.7K views
Variant analysis for mouse WES
variant-calling maftools vcf genome wes
updated by Kevin Blighe Kevin Blighe • written by alba • alba
0 votes
5 replies
9.9K views
Whole Genome Sequencing IG regions
immunoglobulin wgs
updated by Kevin Blighe Kevin Blighe • written by 719 • 719
1 vote
4 replies
8.5K views
Subsetting before QC in Spatial Transcriptomics
spatial-transcriptomics rare-cell-types quality-control
written by Kent • Kent
0 votes
2 replies
7.5K views
How to get a legend from genoplotR?
visualization fastani genome genoplotr
updated by Kevin Blighe Kevin Blighe • written by marongiu.luigi marongiu.luigi
0 votes
5 replies
7.5K views
Trouble when installing arb
arb
updated by Kevin Blighe Kevin Blighe • written by krmoon518 • krmoon518
0 votes
4 replies
7.0K views
Help with species tree with orthofinder, iqtree and branch labeling
iqtree trimal cafe5 orthofinder mafft
written by san96 • san96
0 votes
3 replies
5.7K views
Chimera removal dilemma in 16S V3–V4: necessary when using Kraken2/Bracken?
16s v3-v4 qiime2 kraken2 bracken
updated by GenoMax GenoMax • written by Christopher • Christopher
0 votes
4 replies
3.7K views
Functional prediction for proteins of bacteria.
predection proteins fuctional bacteria
updated by Kevin Blighe Kevin Blighe • written by m90 • m90
0 votes
3 replies
2.4K views
Genome Contamination Check with Blobtools
diamond blobtools genome blast
updated by Kevin Blighe Kevin Blighe • written by saamhasan55 • saamhasan55
0 votes
3 replies
2.0K views
help with modkit on nanopore sequencing data.
mokit nanopore help
written by felix.adams • felix.adams
0 votes
1 reply
1.1K views
Is this the correct order of steps in GATK germline cohort variant calling (VQSR workflow)?
gvcf vcf gatk vqsr
updated by Kevin Blighe Kevin Blighe • written by Ramnaresh • Ramnaresh
0 votes
1 reply
691 views
Forum: Seeking Advice on Bioinformatics Analysis for Atrasentan in IgA Nephropathy Research
bioinformatics atrasentan
updated by Ram Ram • written by capafa1703 • capafa1703
0 votes
6 replies
2.1K views
10x Cell Ranger Multi
10x single cell multiplexing
updated by Kevin Blighe Kevin Blighe • written by zizigolu zizigolu
0 votes
4 replies
1.8K views
Critique or Feedback of filtering strategy for LIANA+ results using spatially informed bivariate metrics
liana spatial-transcriptomics visiumhd
written by danielpintard • danielpintard
0 votes
20 replies
9.9K views
PacBio raw data trimming and cleaning
pacbio trimming cleaning qc
updated by lieven.sterck lieven.sterck • written by misterie misterie
0 votes
0 replies
951 views
News: Online Course - A Practical Introduction to NGS Data Analysis (December 3-5, 2025) - 32% Off
variantcalling illumina workshop dna-seq rna-seq
written by ecSeq Bioinformatics • ecSeq Bioinformatics
0 votes
10 replies
2.2K views
Count unique fragments from a given BAM file
dna bam
updated by GenoMax GenoMax • written by Ankit Ankit
0 votes
2 replies
2.9K views
Merlin ped file issue
linkage merlin
updated by bcvona • bcvona • written by emyli • emyli
0 votes
2 replies
9.5K views
GO Terms significance Scoring
go semantic annotation
updated by geneontologyhelp • geneontologyhelp • written by Marek • Marek
1 vote
2 replies
1.2K views
Ribo-seq Trimming Issue: Massive Read Loss in Paired-end Mode
trimming paired-end riboseq
updated by stuart archer • stuart archer • written by Sammy • Sammy
0 votes
1 reply
601 views
Running snpEff with hg19 reference genome (config file question)
snpeff hg19
updated by Pierre Lindenbaum Pierre Lindenbaum • written by shpak.max • shpak.max
0 votes
3 replies
1.8K views
Error when opening CEL files in python
python
updated by richard.may0810 • richard.may0810 • written by Paula • Paula
0 votes
2 replies
1.2K views
NAT Vs T samples DA analysis
abundance differential microbiome
updated by Kevin Blighe Kevin Blighe • written by San • San
0 votes
1 reply
1.6K views
problem with reading cel files from [HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [HuGene20stv1_Hs_ENTREZG_17.0.0]
normalization microarray
updated by Kevin Blighe Kevin Blighe • written by drwaqasakbar • drwaqasakbar
0 votes
0 replies
802 views
How to select background metabolite sets for differential metabolite enrichment analysis?
enrichment ora
updated by Kevin Blighe Kevin Blighe • written by Edward • Edward
0 votes
1 reply
956 views
How much coverage is needed for high-quality phylogenetic and admixture analyses using short-read WGS in plants?
coverage
updated by Kevin Blighe Kevin Blighe • written by rosaceae • rosaceae
0 votes
2 replies
1.3K views
Does the weights file stay the same for a different model organism
machine-learning
updated by Kevin Blighe Kevin Blighe • written by Sharma • Sharma
0 votes
4 replies
6.4K views
SNP calling with bacterial assembled genome
variant-calling snp bacteria
updated by Kevin Blighe Kevin Blighe • written by yesquokkan • yesquokkan
0 votes
1 reply
1.0K views
Best way to map biological pathways to cancer hallmarks using PLMs (without building models)?
plm mapping llm extraction relation
updated by Kevin Blighe Kevin Blighe • written by DEPANSHI • DEPANSHI
0 votes
1 reply
867 views
Small Molecule Databases and clustering
polyethylene moleculardatabase molecule pet trephthalate
updated by Kevin Blighe Kevin Blighe • written by yarrowmadrona • yarrowmadrona
0 votes
2 replies
1.2K views
Detecting genomic signatures of drug resistance breast cancer in transcriptomic data
data transcriptomic
updated by Kevin Blighe Kevin Blighe • written by olusolaxx • olusolaxx
0 votes
2 replies
1.6K views
Clustal omega on M1 Mac
alignment clustal-omega macos
updated by Kevin Blighe Kevin Blighe • written by kate-nelis • kate-nelis
0 votes
2 replies
1.5K views
Depth and genotype quality in vcf file
vcf depth wes variants
updated by Kevin Blighe Kevin Blighe • written by ashaneev07 • ashaneev07
0 votes
5 replies
2.4K views
Need help with fgsea
scrnaseq fgsea
updated by Kevin Blighe Kevin Blighe • written by bio_info • bio_info
0 votes
3 replies
1.5K views
RNA-seq data compared to qRT-PCR
rna-seq
updated by Kevin Blighe Kevin Blighe • written by SeoG • SeoG
0 votes
3 replies
2.1K views
biomart hangs
biomart
updated by Kevin Blighe Kevin Blighe • written by ramiro.barrantes • ramiro.barrantes
0 votes
7 replies
2.0K views
PCA interpretation
rnaseq pca
updated by Kevin Blighe Kevin Blighe • written by nuriagaralon • nuriagaralon
1 vote
28 replies
9.0K views
Did anyone manage to successfully run HLAScan and get results?
hla-typing ngs
updated by Kevin Blighe Kevin Blighe • written by zeynep • zeynep
0 votes
30 replies
5.2K views
What's the powerful biological methods for significant genes selection?
gene selection significant biological method r
updated by Kevin Blighe Kevin Blighe • written by Chaimaa Chaimaa
0 votes
44 replies
10.0K views
Annotation of huge number of CNV files
cnv annotation tcga
updated by Kevin Blighe Kevin Blighe • written by nazaninhoseinkhan nazaninhoseinkhan
0 votes
46 replies
11K views
SAM file wrong? help with validatesamfile
exome
updated by Kevin Blighe Kevin Blighe • written by cristina_sabiers cristina_sabiers
3 votes
39 replies
21K views
Differential gene Analysis by Limma
limma microarray r
updated by Kevin Blighe Kevin Blighe • written by Mo Mo
1 vote
56 replies
17K views
hash function for DNA sequences
hash hashing dna
updated by Kevin Blighe Kevin Blighe • written by midox midox
1 vote
4 replies
26K views
Subsetting - Raw Counts from Seurat Object
seurat raw counts rna-seq
updated by Kevin Blighe Kevin Blighe • written by David_emir David_emir
1 vote
7 replies
38K views
terminate called after throwing an instance of 'std::bad_alloc?
bedtools linux data
updated by Kevin Blighe Kevin Blighe • written by star star
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