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200k
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105
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31
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95k
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62k
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12k
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updated 7 weeks ago by
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10k
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58
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11k
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30
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15k
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updated 11 months ago by
Ram
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450
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29
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11k
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16
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29
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1.9k
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updated 10 months ago by
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written 8.2 years ago by
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2.5k
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updated 6 months ago by
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Simon Cockell
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39
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28
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11k
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3 months ago by
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43
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updated 6 months ago by
Ram
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written 14.1 years ago by
Eric Normandeau
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29
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28
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35k
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updated 20 days ago by
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written 12.8 years ago by
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Forum:
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written 5.8 years ago by
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197
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35k
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Forum:
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updated 10 months ago by
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written 12.4 years ago by
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82
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27
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21k
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73
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27
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28k
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updated 5 months ago by
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written 10.3 years ago by
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updated 11 months ago by
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96
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Pierre Lindenbaum
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81
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26
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190
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Since a specific region is being amplified it is expected that many of the library fragments will have identical sequences. Thus the low nu…
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@genomax Thank you so much for replying. I was quite unsure about the qualities. One more question. There is no problem within nucleotide d…
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> My specific question is about the feasibility of per base sequence content analysis and whether I should be concerned about a particular …
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But I used the same bam files in both freebayes and GATK. Even the overlapping SNPs from both software are giving different estimates in te…
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may be downsampling(?), QC failing reads ?
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Thank you. However, I extracted the AD for each SNP that gives two numbers separated by a comma. I guess the first one is for reference and…
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can you please retry, but add
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if the CRAM/BAM is paire-end, it **must** be sorted on query name using samtools collate
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one of the issues is that I am not very sure how the sequencing was done, but I think it was single end.
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Possibly. You appear to have written the data to a single file (in case it was paired-end to begin with). Check to make sure secondary alig…
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