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0 votes
6 replies
3.6K views
How to interpret the output from a DESeq2 WALD test analysis in R to compare gene expression in two different tissues.
wald gene-expression-comparison deseq2
written by QuayQ BioMe • QuayQ BioMe
0 votes
1 reply
1.1K views
Job: AN INTEGRATED IN SILICO AND IN VITRO STUDY ON THE DEVELOPMENT OF A COMPUTATIONAL METHOD FOR THE PREDICTION OF O-GLCNAC MODIFIED PROTEINS
proteomics
updated by Ram Ram • written by Anooshian • Anooshian
0 votes
1 reply
861 views
Validate the assembly of unmapped reads by remapping the original reads back to the assembled sequence
unmapped_reads. assembly. paired-end_reads
updated by GenoMax GenoMax • written by Sony Nguyen • Sony Nguyen
0 votes
2 replies
1.3K views
A question about using HISAT2 without biological replicates
rna-seq hisat2
written by vinayjrao vinayjrao
1 vote
0 replies
487 views
How to process Iso-seq subreads in fastq downloaed from NCBI SRA database ?
pacbio
written by 2310691738 • 2310691738
0 votes
0 replies
614 views
Small portion of contigs have high coverage
metagenomics virus read-mapping
updated by Ram Ram • written by Md Moinuddin • Md Moinuddin
1 vote
2 replies
1.3K views
Looking for generative AI applications in bioinformatics
genai chatgpt
updated by Ram Ram • written by a615ebfb • a615ebfb
0 votes
1 reply
1.1K views
How to map CPTAC digital pathology slides to sample ID and metadata
cptac digital-pathology
updated by Zhenyu Zhang Zhenyu Zhang • written by Jakub • Jakub
0 votes
2 replies
1.9K views
installation of deepTools
deeptools deeptools
updated by GenoMax GenoMax • written by Sue • Sue
0 votes
1 reply
738 views
Geo2R - extremely high foldchange
geo2r
updated by ATpoint ATpoint • written by Sanjukta • Sanjukta
2 votes
3 replies
1.8K views
Would a string composed of `#CHROM`, `POS`, `REF` and `ALT` uniquely identify a variant?
vcf
updated by LauferVA LauferVA • written by gernophil • gernophil
0 votes
4 replies
1.4K views
merge regions into one bigger region
genomicranges
written by Chironex • Chironex
0 votes
3 replies
1.2K views
featureCounts output file chromosome identifier issue
featurecounts rnaseq trnascriptome wts sequencing
updated by GenoMax GenoMax • written by tanbiswas6 • tanbiswas6
0 votes
2 replies
1.5K views
command not found: bigwigAverage
deeptools
written by Aki • Aki
0 votes
0 replies
622 views
CellPhoneDB results
cellphonedb cpdb cell_surface_proteins
updated by GenoMax GenoMax • written by piotto • piotto
0 votes
0 replies
618 views
News: Online course: Genome Assembly Using Oxford Nanopore Sequencing - 4 seats remaining
genomeassembly nanopore longreads
written by Physalia-courses Physalia-courses
0 votes
6 replies
2.0K views
Multiple unique reads sharing same UMI barcode
umi ngs
updated by i.sudbery i.sudbery • written by ww22runner • ww22runner
0 votes
1 reply
954 views
How to find clinical data for TARGET AML
target aml
updated by Zhenyu Zhang Zhenyu Zhang • written by veronique.voisin • veronique.voisin
0 votes
0 replies
719 views
Ran both metabat2 and vamb on assembled metagenome sequences, unsure how to interpret differing results between the two
binning metabat2 vamb taxonomy
written by jway • jway
0 votes
4 replies
1.7K views
perplexed regarding the process of gene expression normalision
gene rna-seq expression normalization
written by DS • DS
0 votes
1 reply
890 views
Refseq IDs conversion to gene symbol
ensembl orthology symbol refseq gene
updated by GenoMax GenoMax • written by rgrindle • rgrindle
0 votes
4 replies
1.5K views
How can I get the sequences of the first 20nt of introns and the last 250nt of introns of hg38 genome?
annotation rna
updated by Juke34 Juke34 • written by xiaoleiusc xiaoleiusc
0 votes
2 replies
922 views
Error Reading gencode.v38.genes.json.gz File in SvAnna CLI Tool
structural-variant svanna
written by pragnapcu • pragnapcu
0 votes
3 replies
1.6K views
Rename BAM file
bam
updated by Ram Ram • written by 8armed • 8armed
0 votes
1 reply
1.0K views
picard CreateSequenceDictionary errors when trying to make a .dict file
picard dict
updated by colindaven colindaven • written by michael.flower.14 • michael.flower.14
0 votes
4 replies
1.3K views
False positive RT-PCR in Viral Detection due to Human genome?
false-positive rt-pcr
updated by Ram Ram • written by Naja • Naja
3 votes
0 replies
1.7K views
Tutorial: Premade library preparation – considerations, tips and tricks (IA)
premade-library illumina novaseqx
written by Novogene • Novogene
0 votes
0 replies
586 views
News: Webinar about scRNA-seq cell type prediction
scrnaseq
updated by GenoMax GenoMax • written by jenny • jenny
0 votes
1 reply
1.1K views
How to obtain fine-scale classification from Kraken2
kraken2 sequence metagenome assembly
updated by Fabio Marroni Fabio Marroni • written by anikcropscience • anikcropscience
1 vote
4 replies
1.7K views
Forum: Would reaching out to local labs and startups help with my career transition?
career volunteering
updated by GenoMax GenoMax • written by Mango • Mango
0 votes
4 replies
1.6K views
Should we update time periods in a Bibliometric analysis even after completing it, if it's found to be generating biased results due to recent study?
review bibliometric offtopic
written by Md. Al - Mustanjid • Md. Al - Mustanjid
0 votes
2 replies
1.6K views
PCA results from PLINK and Hail vastly different
gwas plink hail pca
updated by chrchang523 chrchang523 • written by Ahmed • Ahmed
0 votes
6 replies
2.1K views
Making PCA plot using variance instead of counts on Sleuth (plot_pca)
sleuth rnaseq pca
updated by Ram Ram • written by unkown • unkown
0 votes
2 replies
1.5K views
AMRFinderPlus and CARD tools
antimicrobial-resistance genomics
updated by Ram Ram • written by Baka • Baka
0 votes
11 replies
2.9K views
Reference Guided genome assembly
tabix
updated by Michael Dondrup Michael Dondrup • written by sainavyav22 • sainavyav22
0 votes
10 replies
2.6K views
Denovo transcriptome assembly
denovo assembly transcriptome
written by prs • prs
0 votes
2 replies
1.1K views
samtools faidx not finding my modified sequence
genome samtools
updated by ATpoint ATpoint • written by andrebolerbarros • andrebolerbarros
0 votes
0 replies
1.6K views
Error in file braker.pl at line 5404. Failed to execute gmes_petap.pl
perl braker
updated by Ram Ram • written by natt1621 • natt1621
0 votes
2 replies
1.0K views
Excessive Unassigned_FragmentLength in featurecounts
rna-seq featurecounts
updated by ATpoint ATpoint • written by alex • alex
0 votes
2 replies
1.1K views
Read coverage bias
transcripts differential-expression read-coverage
written by ajaymaran97 • ajaymaran97
0 votes
2 replies
1.2K views
Analyzing dataset with counts and TMM values?
tmm counts gene-expression
written by trkfs • trkfs
1 vote
4 replies
3.9K views
Separation of haplotypes in genome assembly
haplotypes pacbio assembly genome reads
written by san96 • san96
0 votes
1 reply
823 views
Using tophat2 output for kallisto
bam tophat2 kallisto
updated by dsull dsull • written by Wintermute • Wintermute
0 votes
2 replies
962 views
Orthomcl Pairs
mysql orthomcl orthomclpairs
written by Harshini • Harshini
1 vote
6 replies
7.3K views
Mouse to Human Protein Alignment Maps
alignment sequence mouse
updated by j.gleixner • j.gleixner • written by Noushin N Noushin N
0 votes
4 replies
4.3K views
trimmomatic: command not found
ubuntu trimmomatic rna-seq linux
updated by bhumm • bhumm • written by n_navy • n_navy
0 votes
1 reply
1.1K views
shotgun metagenomics degs analysis
deg degs shotgun
updated by colindaven colindaven • written by sooni • sooni
0 votes
6 replies
1.8K views
Bcftools view error
bcftools
written by yvyu • yvyu
0 votes
2 replies
1.3K views
genome data size
plink hail regenie
written by kkk • kkk
0 votes
3 replies
1.4K views
Contigs number vs NNN gap % in WGS
wgs
updated by GenoMax GenoMax • written by Pegasus • Pegasus
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