In this case, I only have rna-seq data, is it difficult to execute this SAILOR pipeline?
• 0 views
•
link
Hi everyone, I have data from a variant call in rna-seq and I wanted to check if some of the variants are linked to the rna editing effect, I already researched it and didn't find a satisfactory answer.
abs.
I have followed methods proposed by David Liu and other researchers, such as GATK4, REDITools2, and the SAILOR pipeline. All these reference-genome-based methods without controls have significant drawbacks.
In this case, I only have rna-seq data, is it difficult to execute this SAILOR pipeline?
Log in to answer this question.
As in you found workflows but disagree with the logic? Please provide more information as we don't know what answers you've found unsatisfactory, or why.
In fact, I found more talking about the RNA editing process than tools that can perform this analysis. My question is how I could do it, whether I use bam aligned or the vcf file and which tool I can use. Sorry, I'm not that experienced in bioinformatics.