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188 results • Page
2 of 4
Sort: Views
Rank
Views
Votes
Replies
6
votes
4
replies
331
views
Details on salmon index
Salmon
updated 2 days ago by
Rob
6.6k • written 3 days ago by
Lorenzo
• 0
0
votes
5
replies
326
views
Too many unpaired forward reads found by Trimmomatic
Trimmomatic
6 days ago by
SilhouetteQ
• 0
3
votes
1
reply
325
views
Differential Expression with limma: Contrast and Design Matrix, combinatorial approach valid?
limma
updated 4 days ago by
dariober
14k • written 5 days ago by
Holly
▴ 30
1
vote
4
replies
320
views
Help with Biopython for Beginner
Python
ORF
FASTA
Biopython
updated 1 day ago by
Joe
21k • written 10 days ago by
cput
• 0
3
votes
4
replies
319
views
Would you bother re-mapping RNA-seq data from an old GRCh38 build to a newer version?
RNA-seq
patch
freeze
genome
updated 2 days ago by
Jeremy Leipzig
22k • written 3 days ago by
Ali
• 0
0
votes
4
replies
318
views
How to extract cells of different species after mapping with combined genome?
snRNA-seq
scRNA-seq
updated 2 days ago by
Tony
• 0 • written 3 days ago by
vk
▴ 10
0
votes
6
replies
316
views
ComplexHeatmap - How to change fontsize of rowAnnotation
ComplexHeatmap
1 day ago by
hannes.bongartz
• 0
0
votes
4
replies
313
views
How to handle duplicated genes in TCGA data?
TCGA
GDC
mRNA
updated 1 day ago by
txema.heredia
▴ 130 • written 2 days ago by
Ngrin
• 0
0
votes
4
replies
312
views
Merge clusters in Seurat UMAP
seurat
umap
2 days ago by
kilcdincer
▴ 10
0
votes
2
replies
311
views
Extracting mutation status from MAF files - TCGA
TCGA
maf
mutation
updated 6 days ago by
Zhenyu Zhang
★ 1.2k • written 16 days ago by
yahn
• 0
2
votes
4
replies
307
views
Benchmarking RNASeq Variant Calling Pipeline (Short Reads)
rna-seq
vcf
variant-calling
4 days ago by
Esraa
• 0
1
vote
5
replies
307
views
Can I perform a correlation test with 3 biological replicates per condition?
RNA-Seq
deseq2
updated 2 days ago by
Ram
43k • written 3 days ago by
manuelmourato25
• 0
0
votes
5
replies
302
views
calculating genomic coverage/ base overlap in R
genomics
updated 1 day ago by
1769mkc
★ 1.2k • written 4 days ago by
Xbox_27
• 0
0
votes
2
replies
299
views
Traveler with Infernal mapping failed
r2dt
updated 17 hours ago by
anton.i.petrov
• 0 • written 3 months ago by
Larissa
• 0
1
vote
4
replies
296
views
Filtering VCF files based on VAF giving incorrect results
bcftools
GATK
Bioinformatics
picard
VCF
3 days ago by
Arton
▴ 10
3
votes
3
replies
293
views
Gene Specific coverage from WGS data
WGS
SARS-CoV2
updated 5 days ago by
Ram
43k • written 19 days ago by
Adyasha
• 0
1
vote
4
replies
293
views
Add stats to boxplot in R
stats
R
3 days ago by
Ghada
• 0
1
vote
3
replies
291
views
Using ggplotly in R
ggplot
ggplotly
updated 4 days ago by
jared.andrews07
★ 16k • written 4 days ago by
jen
▴ 10
2
votes
3
replies
291
views
error in fun(x((i)), ...) : only defined on a data frame with all numeric alike variables
studio
Cibersort
R
6 days ago by
Azra
▴ 10
0
votes
5
replies
288
views
bbmerge (bbmap) ~ error with insert size file output
bbtools
bbmerge
bbmap
updated 3 days ago by
GenoMax
142k • written 4 days ago by
chrisk
• 0
3
votes
2
replies
287
views
imputation through beagle
panel
beagle
reference
imputation
5 days ago by
analyst
▴ 50
2
votes
3
replies
283
views
Finding variants within a subset of a BAM file
variant
calling
4 days ago by
ramiro.barrantes
• 0
0
votes
4
replies
282
views
how to treat the replicates while performing WGCNA
clustering
WGCNA
replicates
1 day ago by
mavy
▴ 10
2
votes
5
replies
280
views
What does the 'E%' represent in BUSCO results?
BUSCO
updated 5 days ago by
Philipp Bayer
8.5k • written 5 days ago by
林明德
• 0
1
vote
3
replies
279
views
Tool:
Converting Degree Minutes (DM) to Decimal Degrees (DD) using python script
Minutes
Decimal
offtopic
Degrees
python
updated 2 days ago by
Ram
43k • written 4 days ago by
kuttibiotech2009
▴ 30
1
vote
3
replies
279
views
Is there any way to modify this pie chart ?
pie
ggplot
R
chart
updated 2 days ago by
GenoMax
142k • written 5 days ago by
ohtang7
▴ 40
0
votes
3
replies
279
views
Sam file Header problem
Sam
Header
problem
file
updated 2 days ago by
Pierre Lindenbaum
161k • written 3 days ago by
saifulislam99121
• 0
1
vote
1
reply
276
views
Error in CIBERSORTx ($ operator is invalid for atomic vectors)
CIBERSORTx
cibersort
error
RNA-Seq
deconvolution
updated 17 hours ago by
nguyenn6
• 0 • written 12 weeks ago by
star
▴ 10
2
votes
4
replies
273
views
Can 5' and 3' scRNAseq be processed with the same pipeline?
singlecell
seurat
10XGenomics
scRNAseq
updated 3 days ago by
yura.grabovska
▴ 90 • written 3 days ago by
ev97
▴ 20
1
vote
2
replies
269
views
How to interpret infinite odds ratio?
statistics
5 days ago by
Lukas
• 0
1
vote
5
replies
269
views
Annotating file using bcftools
annotation
plink
bcftools
3 days ago by
kl
▴ 10
0
votes
3
replies
268
views
RNAseq RNA content
mRNA
Linux
rRNA
RNA-seq
RNA
updated 4 days ago by
noodle
▴ 580 • written 5 days ago by
doramora
▴ 10
0
votes
4
replies
263
views
Galaxy StringTie error
stringtie
galaxy
5 days ago by
trkfs
• 0
2
votes
3
replies
253
views
ggrepel function: geom_text_repel()
ggplot2
ggrepel
R
updated 2 days ago by
Ram
43k • written 3 days ago by
sooni
▴ 20
2
votes
2
replies
251
views
Single-cell ambient RNA correction: SoupX vs decontX contamination fraction
single-cell
ambient-RNA
updated 2 days ago by
Ram
43k • written 2 days ago by
txema.heredia
▴ 130
1
vote
1
reply
249
views
Calculate allelic frequency from VEP output vcf file
VEP
variant
3 days ago by
ramiro.barrantes
• 0
0
votes
1
reply
247
views
Two-Sample Mendelian Randomization: Association between Instrumental Variable and Outcome
Mendelian-Randomization
updated 5 days ago by
Ram
43k • written 9 days ago by
Nikki
• 0
0
votes
4
replies
244
views
Sequencing Depth (Read Depth) Calculations
depth
3 days ago by
LucisTheFather
• 0
0
votes
1
reply
244
views
Nomalization - TCGA, RNA-seq and Microarray
TCGA
RNA-seq
Nomalization
Microarray
updated 6 days ago by
Zhenyu Zhang
★ 1.2k • written 9 days ago by
jain72744
▴ 10
3
votes
3
replies
238
views
getting exon coordinates
genomics
updated 3 days ago by
Ram
43k • written 3 days ago by
Xbox_27
• 0
0
votes
2
replies
235
views
java.nio.bufferunderflowexception haplotypecaller error for bqsr reads
deducplicated
java.nio.bufferunderflowexception
haplotypecaller
bqsr
6 days ago by
analyst
▴ 50
1
vote
2
replies
234
views
Doubt about the process of annotation, detection, identification and classification of transposable elements
annotation
TransposableElements
3 days ago by
MarcosCosta
• 0
0
votes
2
replies
233
views
Obtaining Infinium Methylation450K manifest for hg38
TCGA
hg38
methylation
Illumina
updated 6 days ago by
Zhenyu Zhang
★ 1.2k • written 10 days ago by
elisheva
▴ 120
3
votes
3
replies
232
views
Getting differential ChIP-seq peaks between conditions after calling consensus peaks
MAnorm
ChIP-seq
consensus
peaks
updated 3 days ago by
ATpoint
82k • written 4 days ago by
Alexandra
• 0
2
votes
4
replies
231
views
Help with IGV abbreviation
igv
updated 4 days ago by
Ram
43k • written 5 days ago by
GeneC
• 0
1
vote
2
replies
231
views
Why does assigning genes with biomart give me different values than using a transcripts_to_genes.txt file?
biomart
RNAseq
kallisto
2 days ago by
bioinfo
▴ 150
0
votes
2
replies
229
views
Inquiry about deseq2 transformation
RNA-seq
deseq2
3 days ago by
Chen
• 0
1
vote
5
replies
223
views
Circos Plot to Visualize Chromatin Accessibility Changes from ATAC-seq Data
circos
atacseq
updated 4 days ago by
Pierre Lindenbaum
161k • written 4 days ago by
biology_inform
▴ 50
0
votes
2
replies
220
views
How to generate table_annovar from VCF input?
vcf
annovar
updated 2 days ago by
Pierre Lindenbaum
161k • written 2 days ago by
melissachua90
▴ 70
3
votes
1
reply
218
views
Long reads and fixing of mate-pair issues/marking duplicates with samtools
mate-pair
samtools
alignment
updated 6 days ago by
aw7
▴ 300 • written 10 days ago by
Zeng Hao
▴ 40
188 results • Page
2 of 4
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plotting trees in R: node labels from nexus tree files
Interpreting the Jackstraw plot of Seurat
A: How is the design in DESeq2 work?
Error in CIBERSORTx ($ operator is invalid for atomic vectors)
Answer: RNAseq coverage vs depth for transcript isoform expression?
constructing pangenome through psvcp
Differential Expression with limma: Contrast and Design Matrix, combinatorial approach valid?
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Comment: What marks a De-Novo Genome assembly as FAILED?
by
Umer
▴ 50
Yes. We are sequencing 45 different samples with illumina. 12 of these are also going to be sequenced with Nanopore (for hybrid assembly). …
Comment: HGVS Nomenclature of Multiple Indels found in Cis
by
LauferVA
4.2k
how has this code progressed? is it publically available? would you want to work together?
Comment: Design for complex RNA-Seq experiment using Deseq2
by
Ezequiel
• 0
I completely agree with most points. The PCA shows that the biggest driver of difference is the patients themselves, with PRE and POST time…
Comment: Add stats to the plot
by
marco.barr
▴ 130
Hi, you can add statistical annotations using `ggpubr` function `stat_pvalue_manual `. You need to make sure you calculate the p-values fir…
Answer: seqtk subseq in.fastq list.txt > out.fastq not extracting full sequence from
by
ATpoint
82k
Use everything before the whitespace in list.txt, for example `A00869:294:HCW57DSXY:2:1101:3929:1000` and not `A00869:294:HCW57DSXY:2:1101:…
Answer: CellRanger output more cells than specified using --force-cells? Why?
by
scideas
▴ 30
I actually had this same issue with CellRanger v7- turns out that since you have 2 species in your mapping reference, CellRanger is forcing…
Answer: Checking chromosome builds for genotyping data
by
Muhammad
• 0
Assume you have genotype data in Plink format. (G.bed, G.bim, G.fam) Download some reference panel data in Plink format (R.bed, R.bim, R.f…
Comment: Error in CIBERSORTx ($ operator is invalid for atomic vectors)
by
nguyenn6
• 0
did you ever figure it out? i'm having the same issue.
Comment: Traveler with Infernal mapping failed
by
anton.i.petrov
• 0
Hi Larissa! I am the lead developer of R2DT and I've just noticed this question. Could you please email help@r2dt.bio with your input seque…
Comment: constructing pangenome through psvcp
by
analyst
▴ 50
Fixed the issue by updating nucmer version.
Comment: Blastn error : ncbi::CObject::ThrowNullPointerException() - Attempt to access NU
by
GenoMax
142k
Can you create a small test database (`makeblastdb`) from your query file and try to run the search against the same file and see if that w…
Comment: calculating genomic coverage/ base overlap in R
by
1769mkc
★ 1.2k
you can `dput(head(df)` for the both the data frame so that other can test for possible solutions
Comment: Broad and Narrow peaks
by
ATpoint
82k
What is your question? It's unclear.
Comment: Help with Biopython for Beginner
by
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21k
One question before offering more solutions: are you trying to learn Python with this as an example problem, or are you trying to find the …
Answer: RNAseq coverage vs depth for transcript isoform expression?
by
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★ 7.2k
See the exploration of isoform estimation precision vs read length and sequencing depth in our paper: https://doi.org/10.1093/nar/gkad1167.…
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