Latest
Open
Jobs
Tutorials
Tags
About
FAQ
Community
Planet
New Post
Log In
New Post
Latest
Open
News
Jobs
Tutorials
Forum
Tags
Planet
Users
Log In
Sign Up
About
Limit : this year
all time
today
this week
this month
this year
12,116 results • Page
2 of 243
Sort: replies
Rank
Views
Votes
Replies
11
votes
31
replies
15k
views
6 follow
How to concatenate multiple fasta file
sequence
genome
updated 11 months ago by
doggie
• 0 • written 5.8 years ago by
fec2
▴ 50
120
votes
31
replies
200k
views
17 follow
Correct Way To Parse A Fasta File In Python
python
fasta
updated 6 months ago by
Ram
44k • written 14.1 years ago by
Eric Normandeau
11k
147
votes
31
replies
138k
views
26 follow
Useful Bash Commands To Handle Fasta Files
command-line
fasta
bash
updated 10 months ago by
Ram
44k • written 12.3 years ago by
Anima Mundi
★ 2.9k
50
votes
30
replies
12k
views
13 follow
Tutorial:
How to Use Biostars, Part-I: Questions, Answers, Comments and Replies
documentation
meta
how-to
updated 8 weeks ago by
Istvan Albert
100k • written 8.2 years ago by
Ram
44k
31
votes
30
replies
15k
views
6 follow
Tool:
BioVinci: user-friendly software to make plotting easy - PCA, 3D scatter plot, venn diagram, you name it.
gene
RNA-Seq
Venn-diagram
PCA
updated 11 months ago by
Ram
44k • written 6.0 years ago by
Jennifer Pham
▴ 450
18
votes
30
replies
10k
views
6 follow
Tool:
Introducing FilterByTile: Remove Low-Quality Reads Without Adding Bias
Illumina
BBMap
filterbytile
updated 11 months ago by
Ram
44k • written 7.4 years ago by
Brian Bushnell
20k
58
votes
30
replies
11k
views
13 follow
Content Management Systems For Bioinformatics Websites
web
updated 6 months ago by
Ram
44k • written 14.1 years ago by
Khader Shameer
18k
102
votes
30
replies
62k
views
25 follow
Tools To Find Gene Ontology Term Enrichment
gene
enrichment
updated 7 months ago by
Ram
44k • written 14.2 years ago by
Biostar User
★ 1.0k
4
votes
29
replies
11k
views
6 follow
WGCNA soft thresholding problem
WGCNA
RNA-Seq
soft thresholding
data normalization
updated 12 weeks ago by
foejvs546
▴ 10 • written 5.5 years ago by
catagui
▴ 40
124
votes
29
replies
7.4k
views
18 follow
What License Do You Use When You Release Code And Data?
software
general
subjective
updated 6 months ago by
Ram
44k • written 14.2 years ago by
Simon Cockell
7.4k
16
votes
29
replies
1.9k
views
Gene labels problem in enhanced volcano
R
ggplot2
enhancedvolcano
updated 3 months ago by
Mensur Dlakic
★ 27k • written 3 months ago by
anasjamshed
▴ 120
84
votes
29
replies
32k
views
20 follow
Tutorial:
How to create a mutation landscape (waterfall) plot with GenVisR
BioConductor
GenVisR
Graphics
R
updated 10 months ago by
amziepickles
• 0 • written 8.2 years ago by
Obi Griffith
20k
20
votes
29
replies
2.6k
views
6 follow
Using bcftools to find unique alt homozygous sites
bcftools
updated 7 months ago by
Pierre Lindenbaum
162k • written 7 months ago by
Axzd
▴ 70
70
votes
29
replies
15k
views
13 follow
Tutorial:
Generating consensus sequence from bam file
fasta
consensus
bam
updated 10 weeks ago by
jkbonfield
★ 1.2k • written 5.2 years ago by
finswimmer
16k
39
votes
28
replies
11k
views
10 follow
How Much Does It Cost To Align A Flowcell In The Cloud?
cloud
3 months ago by
Jeremy Leipzig
22k
43
votes
28
replies
5.7k
views
13 follow
Post Your Prefered Bioinformatics Short Code
code
updated 6 months ago by
Ram
44k • written 14.1 years ago by
Eric Normandeau
11k
29
votes
28
replies
35k
views
11 follow
Split Fastq Files Into Chunks Of 1M Reads
split
fastq
updated 21 days ago by
thomas.heigl.ibk
• 0 • written 12.8 years ago by
Bioscientist
★ 1.7k
197
votes
27
replies
35k
views
20 follow
Forum:
Bioinformatics Cartoon
bioinformatics
updated 10 months ago by
Ram
44k • written 12.4 years ago by
Maxime Lamontagne
★ 2.3k
73
votes
27
replies
28k
views
16 follow
Tutorial:
How To Separate Illumina Based Strand Specific Rna-Seq Alignments By Strand
RNA-seq
updated 5 months ago by
snardeli
• 0 • written 10.3 years ago by
Istvan Albert
100k
26
votes
27
replies
2.2k
views
10 follow
Forum:
Determining computer time required
computer
hpc
time
updated 4 months ago by
dsull
★ 6.1k • written 4 months ago by
Rozita
▴ 40
82
votes
27
replies
21k
views
12 follow
ATAC-seq sample normalization
ChIP-Seq
atac-seq
normalization
updated 7 weeks ago by
sgv
• 0 • written 4.4 years ago by
Flo
▴ 250
27
votes
27
replies
10k
views
6 follow
How To Detect And Query Poly-Allelic Snps?
snp
allele
biomart
dbsnp
updated 6 months ago by
Ram
44k • written 14.2 years ago by
Michael
54k
28
votes
27
replies
15k
views
9 follow
Extracting A Subset Of Sequences From A Fastq File (Biopython Speed)
biopython
python
fastq
next-gen-sequencing
updated 6 months ago by
Ram
44k • written 14.1 years ago by
Darked89
4.6k
77
votes
26
replies
121k
views
15 follow
How to count fastq reads
sequence
next-gen
fastq
reads
updated 6 months ago by
cschu181
★ 2.8k • written 9.1 years ago by
Chenglin
▴ 260
81
votes
26
replies
13k
views
17 follow
Appropriate Podcasts For A Bioinformatician?
subjective
updated 7 months ago by
Ram
44k • written 14.2 years ago by
User 59
13k
24
votes
26
replies
3.7k
views
Tool:
ClinCNV: CNV detection from short reads
variant-calling
cna
cnv
updated 11 months ago by
Ram
44k • written 4.6 years ago by
German.M.Demidov
★ 2.9k
20
votes
26
replies
3.3k
views
Is there a command line tool that can take an alignment fasta and input and outputs an image?
alignment
fasta
image
updated 9 months ago by
Joe
21k • written 4.9 years ago by
Joel Wallenius
▴ 210
96
votes
26
replies
31k
views
15 follow
What Methods Do You Use For In/Del/Snp Calling?
short-read-aligner
snp
sequencing
updated 6 months ago by
Ram
44k • written 14.1 years ago by
Pierre Lindenbaum
162k
3
votes
26
replies
2.1k
views
6 follow
STAR Genome index Error
STAR
updated 7 months ago by
Ram
44k • written 7 months ago by
Prasanna
• 0
47
votes
26
replies
4.2k
views
11 follow
Blog:
Evolution of Biostars
meta
Biostars
updated 9 months ago by
aldhairmedico
▴ 70 • written 5.2 years ago by
venu
7.1k
38
votes
26
replies
19k
views
16 follow
GO analysis: DAVID vs GREAT vs GOrilla
RNA-Seq
ChIP-Seq
GO
gene ontology
written 8.3 years ago by
biostart
▴ 370
105
votes
26
replies
43k
views
23 follow
How To Create Mutation Diagram In R Or In Any Tools?
mutation
domain
r
updated 8 months ago by
zx8754
11k • written 11.4 years ago by
henryvuong
▴ 810
21
votes
26
replies
9.8k
views
11 follow
Tutorial:
Create de novo repeat library
de-novo
repeat
annotation
updated 10 weeks ago by
Yao
▴ 30 • written 4.5 years ago by
Juke34
8.6k
7
votes
25
replies
11k
views
6 follow
Tool:
MIGEC: towards error-free profiling of immune repertoires
sequencing-error
tcr
antibody
molecular-barcode
updated 11 months ago by
Ram
44k • written 10.1 years ago by
mikhail.shugay
3.5k
69
votes
25
replies
7.1k
views
16 follow
How Do You Explain What You Do To The Guy On The Street Or Your Mum?
general
updated 6 months ago by
Ram
44k • written 14.2 years ago by
Dave Gerrard
▴ 190
51
votes
25
replies
6.9k
views
10 follow
What Is Your Experience With Bioinformatics Webservices?
web-service
subjective
updated 6 months ago by
Ram
44k • written 14.2 years ago by
Michael
54k
13
votes
25
replies
11k
views
9 follow
Tool:
Mitcr: A Software Tool For Analyzing T-Cell Receptor Sequencing Data
analysis
sequencing
ngs
updated 11 months ago by
Ram
44k • written 10.8 years ago by
mikhail.shugay
3.5k
6
votes
25
replies
39k
views
14 follow
CluserProfiler message "No gene can be mapped"
R
updated 11 months ago by
13554221497
• 0 • written 6.2 years ago by
ARich
▴ 130
38
votes
25
replies
9.2k
views
13 follow
FASTA file of fixed length
sequence
fasta
updated 8 months ago by
Ram
44k • written 8.1 years ago by
waqasnayab
▴ 250
36
votes
25
replies
33k
views
15 follow
Downsampling dataset with more than 60 million reads
next-gen
RNA-Seq
updated 5 months ago by
asalimih
▴ 60 • written 9.8 years ago by
mike
▴ 90
68
votes
25
replies
16k
views
12 follow
Tool:
sra-explorer : find SRA and FastQ download URLs in a couple of clicks
fastq
SRA
updated 7 months ago by
GenoMax
142k • written 5.2 years ago by
Phil Ewels
★ 1.4k
18
votes
25
replies
15k
views
13 follow
Easy way to split VCF file by chromosome
sequence
TOPMED
chromosome
Imputation
SNP
updated 10 months ago by
Pierre Lindenbaum
162k • written 2.3 years ago by
ConvolutedGenome
▴ 30
13
votes
25
replies
4.4k
views
7 follow
VEP output has no gene names
alignment
sequencing
vep
updated 11 months ago by
barslmn
★ 2.2k • written 5.8 years ago by
Gene_MMP8
▴ 240
17
votes
25
replies
2.5k
views
Tool:
Platform for Bioinformatics Students, Scholars and Scientists
sequence
genome
updated 11 months ago by
Ram
44k • written 8.2 years ago by
support
▴ 50
60
votes
25
replies
5.9k
views
8 follow
Forum:
Am I crazy, or are most published RNA-seq studies vastly underpowered?
sample-size
statistical-power
RNA-Seq
updated 11 months ago by
Ram
44k • written 3.8 years ago by
telroyjatter
▴ 230
60
votes
25
replies
22k
views
17 follow
Which Operating System Do You Prefer For Bioinformatics?
general
subjective
updated 7 months ago by
Ram
44k • written 14.3 years ago by
Giovanni M Dall'Olio
28k
64
votes
24
replies
3.1k
views
12 follow
Forum:
How much I must feel useless?
machine-learning
Programming
statistics
updated 12 months ago by
Ram
44k • written 5.6 years ago by
Molecular_genetics
▴ 80
87
votes
24
replies
24k
views
17 follow
Tutorial:
Gene Set Enrichment Analysis
microarray
enrichment
RNA-Seq
GSEA
updated 5 weeks ago by
Gordon
• 0 • written 3.6 years ago by
Hamid Ghaedi
3.2k
80
votes
24
replies
11k
views
18 follow
Which Bioinformatics Journals Do You Follow?
bioinformatics-journal
updated 6 months ago by
Ram
44k • written 14.1 years ago by
Giovanni M Dall'Olio
28k
27
votes
24
replies
2.5k
views
7 follow
Code Golf - bisulfite conversion
bisulfite
bsseq
code-golf
updated 12 months ago by
Ram
44k • written 5.6 years ago by
Chris Miller
22k
12,116 results • Page
2 of 243
Recent Votes
Random Access remote BAM files
Answer: PCA plot no distinct cluster. Only p < 0.05 indicates significant DEGs, while ad
C: Converting narrowPeak to bed
Answer: PCA plot no distinct cluster. Only p < 0.05 indicates significant DEGs, while ad
Answer: PCA plot no distinct cluster. Only p < 0.05 indicates significant DEGs, while ad
A: VCF files: Change Chromosome Notation
Comment: Z score
Recent Locations •
All
Turkey,
5 minutes ago
Germany,
10 minutes ago
United States,
14 minutes ago
United States,
17 minutes ago
Moscow,
18 minutes ago
Germany,
18 minutes ago
Italy,
20 minutes ago
Recent Awards •
All
Popular Question
to
Erika
• 0
Teacher
to
GenoMax
142k
Popular Question
to
bioinfo
▴ 150
Popular Question
to
a.bibek52
▴ 10
Teacher
to
dsull
★ 6.1k
Popular Question
to
adarsh_pp
▴ 40
Scholar
to
LChart
3.9k
Recent Replies
Comment: What is a good way to do gene differentials in single cell data where one group
by
ATpoint
82k
Preferred if you have true biological replicates. Can still be combined with my subsetting strategy, like use 100x different cells for the …
Answer: ComplexHeatmap - How to change fontsize of rowAnnotation
by
ATpoint
82k
```r set.seed(1) m = matrix(rnorm(100), nrow = 10) rownames(m) = 1:10 # normal ha = rowAnnotation(foo = anno_mark(at=1:nrow(m), labels=row…
Comment: ComplexHeatmap - How to change fontsize of rowAnnotation
by
Ram
44k
Please do not add answers unless you're actually answering the question. I've moved your post to a comment.
Comment: ComplexHeatmap - How to change fontsize of rowAnnotation
by
hannes.bongartz
• 0
I still cannot find a way. It seems to be an easy to solve issue and I was trying any possible combination with the aforementioned gp=gpar(…
Comment: What is a good way to do gene differentials in single cell data where one group
by
fracarb8
★ 1.6k
I would probably use a pseudobulk approach.
Comment: Error when looping over multiple columns in a data frame in R
by
Jeremy
▴ 910
`cutpoint_results[[col_name]]` adds each new cut point to the list entitled "cutpoint_results", while keeping the original column names fr…
Answer: PCA plot no distinct cluster. Only p < 0.05 indicates significant DEGs, while ad
by
swbarnes2
14k
Never use uncorrected p-values with RNASeq. Your PCA is suggesting that your sample groups are not very different, and the DEG tests con…
Comment: What is a good way to do gene differentials in single cell data where one group
by
ATpoint
82k
I would keep it transparent. Do DE by subsetting large to small group. Do that randomly many times, then either average stats or use some s…
Answer: Trying to understand warning from MACS2 about too few paired peaks and differing
by
jared.andrews07
★ 17k
Without looking at the actual sequence for each genome, my guess is that the Echinobase reference just matches much more closely to the org…
Comment: What do the transcript variant # mean in RefSeq?
by
Ram
44k
> It does not seem to be the longest transcript. Is it the canonical transcript? Longest == canonical. If you're looking for the transcrip…
Comment: Mouse or Rat Gene Expression Data Similar to GTEx
by
Shicheng Guo
★ 9.4k
Hi ATpoint, Thank you for your responses. My apologies if my inquiries have seemed one-sided. I greatly value the collaborative spirit of t…
Comment: Mouse or Rat Gene Expression Data Similar to GTEx
by
ATpoint
82k
Team? A team helps each other out. You only take help by posting your regular lazy do-my-work questions and never give anything back. Save …
Comment: What do the transcript variant # mean in RefSeq?
by
GenoMax
142k
> What do these correspond to? It does not seem to be the longest transcript. Is it the canonical transcript? Those are just different tra…
Answer: Mouse or Rat Gene Expression Data Similar to GTEx
by
jared.andrews07
★ 17k
MGI has a [Gene Expression Database](https://www.informatics.jax.org/expression.shtml) that'll largely get you what you want.
Comment: Difference in Bismark output methylation call files and coverage files
by
Papyrus
★ 2.9k
Your files end in `CpG_report` so they are the "genome-wide cytosine report output". If you look at the [Bismark documentation][1] you will…
Traffic: 1642 users visited in the last hour
Content
Search
Users
Tags
Badges
Help
About
FAQ
Access
RSS
API
Stats
Use of this site constitutes acceptance of our
User Agreement and Privacy Policy
.
Powered by the
version 2.3.6