just now by
hannes.bongartz
•
0
0
votes
0
replies
1
view
1
vote
4
replies
130
views
0
votes
0
replies
20
views
updated 1 hour ago by
GenoMax
142k
•
written 2 hours ago by
yura.grabovska
▴
90
4
votes
7
replies
375
views
updated 1 hour ago by
marco.barr
▴
130
•
written 1 day ago by
diqixiaoyaoer
▴
20
0
votes
0
replies
42
views
1
vote
8
replies
162
views
0
votes
1
reply
71
views
0
votes
1
reply
107
views
updated 6 hours ago by
txema.heredia
▴
130
•
written 14 hours ago by
Ngrin
•
0
4
votes
9
replies
346
views
0
votes
2
replies
138
views
updated 6 hours ago by
Pierre Lindenbaum
161k
•
written 12 hours ago by
melissachua90
▴
70
0
votes
0
replies
50
views
0
votes
1
reply
143
views
updated 4 hours ago by
GenoMax
142k
•
written 11 hours ago by
huxiangyulove
•
0
0
votes
0
replies
68
views
4
votes
5
replies
4.3k
views
updated 15 hours ago by
dario.garvan
▴
520
•
written 7.2 years ago by
d-cameron
★
2.9k
0
votes
4
replies
239
views
6 hours ago by
kilcdincer
▴
10
0
votes
0
replies
72
views
20 hours ago by
sainavyav22
•
0
2
votes
2
replies
181
views
updated 18 hours ago by
Ram
43k
•
written 21 hours ago by
txema.heredia
▴
130
0
votes
0
replies
85
views
updated 15 hours ago by
Ram
43k
•
written 22 hours ago by
carlopecoraro2
★
2.5k
5
votes
4
replies
230
views
0
votes
0
replies
78
views
0
votes
1
reply
127
views
1
vote
1
reply
135
views
0
votes
0
replies
78
views
3
votes
4
replies
255
views
3
votes
3
replies
174
views
0
votes
4
replies
252
views
1
vote
5
replies
231
views
updated 15 hours ago by
Ram
43k
•
written 1 day ago by
manuelmourato25
•
0
2
votes
11
replies
632
views
1
vote
1
reply
136
views
updated 11 hours ago by
Nyksubuz
▴
20
•
written 1 day ago by
mirwa.zidi93
•
0
0
votes
3
replies
216
views
updated 7 hours ago by
Pierre Lindenbaum
161k
•
written 1 day ago by
saifulislam99121
•
0
1
vote
4
replies
219
views
1
vote
1
reply
148
views
0
votes
1
reply
127
views
2
votes
3
replies
207
views
0
votes
1
reply
143
views
1
vote
14
replies
609
views
0
votes
4
replies
244
views
1
vote
2
replies
222
views
1
vote
3
replies
268
views
1
vote
1
reply
184
views
updated 19 hours ago by
Jordan M Eizenga
▴
460
•
written 11 days ago by
sarumonsus
▴
10
1
vote
3
replies
795
views
updated 20 hours ago by
sansan_96
▴
90
•
written 2.7 years ago by
boymin2020
▴
80
Recent Votes
Recent Locations •
All
United Kingdom, 2 minutes ago
Spain, 2 minutes ago
United Kingdom, 3 minutes ago
Stony Brook University, NY, 6 minutes ago
United States, 7 minutes ago
Italy, 7 minutes ago
United States, 7 minutes ago
Recent Awards •
All
Popular Question to
ashkan
▴
160
Popular Question to
Nyksubuz
▴
20
Popular Question to
Govardhan Anande
▴
150
Recent Replies
Comment: Help! Reconstruction of locus, order contigs
by
dthorbur
★
2.0k
I don't understand your comment. What do you mean?
> my idea is not to have to write it down
If you have an alignment already, why can't…
Comment: Help! Reconstruction of locus, order contigs
by
GenoMax
142k
Please use `ADD REPLY/ADD COMMENT` when responding to existing content. `SUBMIT ANSWER` is for new answers to the original question.
Comment: Details on salmon index
by
Rob
6.6k
Hi Lorenzo,
That is correct. The initial version of salmon used the RapMap index explained above. When we published the selective align…
Comment: Help! Reconstruction of locus, order contigs
by
BATMAN
•
0
Thanks for your reply, my idea is not to have to write it down. What I have is an alignment of the .gbk from the progressiveMauve. It would…
Comment: Faster Needleman-Wunsch rapid global alignment of two sequences?
by
Gabriel R.
★
2.9k
I did some digging aroumd, no static binary. Plus it seems you need to roll your own program.
Comment: Faster Needleman-Wunsch rapid global alignment of two sequences?
by
Gabriel R.
★
2.9k
We tested it, however, it does not seem to produce a fast a file of the results or anything that we can easily parse. It produces a CIGAR a…
Comment: zero TPM for many gene including housekeeping when using kallisto
by
ashkan
▴
160
@atpoint yes but for example for the kallisto results I have expression for 2080 genes (transcripts) and the rest are 0 but in my STAR resu…
Comment: In one PCA plot, can I calculate the percentage of different factors that contri
by
marco.barr
▴
130
great team work! why can't you access and do the calculation for the other components too? can't you extract the information from the other…
Comment: zero TPM for many gene including housekeeping when using kallisto
by
ATpoint
82k
Yes, but this is what I am saying: GAPDH in human has at least 6 annotated transcripts. Have you looked at all of them? Maybe that particul…
Comment: zero TPM for many gene including housekeeping when using kallisto
by
ashkan
▴
160
@atpoint these are from PBMC. no they are different genes. for example ENST00000229239.10 is GAPDH. in STAR I got a lot of reads aligned …
Comment: zero TPM for many gene including housekeeping when using kallisto
by
ashkan
▴
160
@dariober: I have to use kallisto for our downstream pipeline.
Comment: zero TPM for many gene including housekeeping when using kallisto
by
ATpoint
82k
Which celltype is this? Are these transcripts of the same gene, and of which gene?
Comment: zero TPM for many gene including housekeeping when using kallisto
by
ashkan
▴
160
@atpoint the question is updated. the first command shows how the index is built.
Comment: Help! Reconstruction of locus, order contigs
by
dthorbur
★
2.0k
Is the *de novo* annotation required for each contig? Reconstructing homologous regions in many genomes can be tricky regardless, but is es…
Comment: In one PCA plot, can I calculate the percentage of different factors that contri
by
diqixiaoyaoer
▴
20
You can have a look on my answer. I have post it below.
Traffic: 2719 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6