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460 results • Page
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Votes
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3
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3
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927
views
Forum:
Importance of Data Structures for Bioinformatics?
data-structures
updated 7 months ago by
Ram
43k • written 7 months ago by
S
• 0
19
votes
14
replies
2.1k
views
Forum:
What is the amount of sequencing data produced annually?
data
research
sequencing
updated 7 months ago by
Jeremy Leipzig
22k • written 7 months ago by
vincenthus
▴ 70
19
votes
10
replies
1.5k
views
Forum:
Pain points using commercial clouds
gpt
tinybio
updated 7 months ago by
vincenthus
▴ 70 • written 8 months ago by
Sasha
▴ 840
9
votes
6
replies
1.9k
views
6 follow
Forum:
GTF files from Ensembl Releases 105 and 106 unsorted
Ensembl
bug
GTF
updated 7 months ago by
ATpoint
82k • written 17 months ago by
dlaehnemann
▴ 30
3
votes
2
replies
630
views
Forum:
How to contribute to the next edition of Biostars Handbook
handbook
updated 7 months ago by
Ram
43k • written 7 months ago by
Fouad
▴ 20
3
votes
6
replies
1.5k
views
6 follow
Forum:
Is Bioinformatics a Professional Degree?
career
degree
8 months ago by
anasjamshed
▴ 120
2
votes
5
replies
956
views
6 follow
Forum:
Biostar as a next generation version
meta
mobile-app
updated 6 months ago by
Ram
43k • written 8 months ago by
rj.rezwan
• 0
2
votes
9
replies
1.2k
views
Forum:
open source projects looking for contributors
open-source
updated 7 months ago by
vincenthus
▴ 70 • written 8 months ago by
claudiofr
▴ 20
0
votes
0
replies
541
views
Forum:
Recruiters: what do you expect on a junior bioinformatician?
career
skills
updated 8 months ago by
Ram
43k • written 8 months ago by
gallardodiazmiriam
▴ 20
3
votes
4
replies
1.4k
views
Forum:
Discussion: Uploading terabytes of data to NCBI SRA
NCBI
WGS
archiving
servers
remote
updated 8 months ago by
GenoMax
142k • written 8 months ago by
James Reeve
▴ 130
0
votes
0
replies
578
views
Forum:
I have a Master's and little experience in Bioinformatics - what would be the next best step?
career
updated 8 months ago by
Ram
43k • written 8 months ago by
RM123
▴ 10
1
vote
3
replies
793
views
Forum:
Spaceranger missing spatial coordinates [Discussion]
Visium
updated 8 months ago by
GenoMax
142k • written 8 months ago by
morovatunc
▴ 550
11
votes
8
replies
1.4k
views
6 follow
Forum:
How to write a review article in bioinformatics
article
review
updated 8 months ago by
Ram
43k • written 8 months ago by
joey
▴ 10
365
votes
58
replies
69k
views
44 follow
Forum:
Recommend Your Favorite Bioinformatics Books
education
books
updated 6 months ago by
Ram
43k • written 14.2 years ago by
Istvan Albert
100k
1
vote
2
replies
18k
views
Forum:
Would you rather analyze a dataset with a strong, non-collinear batch effect, or a weak, collinear batch effect.
collinear
latent
batch-effect
variable
GLM
9 months ago by
LauferVA
4.2k
0
votes
3
replies
3.8k
views
10 follow
Forum:
Discussion question: Cutoff for SNP phylogeny
Phylogenetics
SNP
written 17 months ago by
SushiRoll
▴ 120
2
votes
3
replies
800
views
Forum:
Purely analytic or in silico manuscripts that have significantly improved biological understanding
dry-lab
updated 9 months ago by
GenoMax
142k • written 10 months ago by
LauferVA
4.2k
0
votes
2
replies
1.3k
views
Forum:
[Detailed Question] How to Normalize of Metagenomics Gene Abundances (with HMMs models)
normalization
updated 10 months ago by
Asaf
10k • written 10 months ago by
Davide
• 0
33
votes
23
replies
4.2k
views
8 follow
Forum:
Is there a file format better suited for the era of pangenomics than the .vcf? What are its attributes?
VCF
file-format
genomics
updated 10 months ago by
Jeremy Leipzig
22k • written 10 months ago by
LauferVA
4.2k
7
votes
8
replies
2.0k
views
Forum:
Would solving many Rosalind problems show your ability for this field in job-wise?
rosalind
career
10 months ago by
K
• 0
2
votes
3
replies
868
views
Forum:
Mapping a predicted sequence to a genome
predicted
map
alignment
sequence
updated 10 months ago by
Mensur Dlakic
★ 27k • written 10 months ago by
Andy
• 0
0
votes
0
replies
448
views
Forum:
Recommendation sought for differential exon usage of a single gene
splicing
differential-exon-usage
updated 11 months ago by
Ram
43k • written 11 months ago by
nick_b55
▴ 10
7
votes
4
replies
1.1k
views
Forum:
[Meta] Use of AI
AI
Artificial-Intelligence
meta
ChatGPT
updated 11 months ago by
Ram
43k • written 11 months ago by
LauferVA
4.2k
2
votes
2
replies
607
views
Forum:
Colleges
Colleges
india
universities
updated 11 months ago by
Ram
43k • written 11 months ago by
mahimanepalli36
• 0
19
votes
15
replies
2.0k
views
6 follow
Forum:
Cross out line in Biostars
meta
updated 11 months ago by
Ram
43k • written 6.0 years ago by
Bastien Hervé
5.3k
121
votes
33
replies
39k
views
24 follow
Forum:
Where To Look For Quality Bioinformatics Short Courses And Workshops?
Courses
updated 12 months ago by
carlopecoraro2
★ 2.5k • written 10.7 years ago by
Eric Normandeau
11k
103
votes
42
replies
31k
views
34 follow
Forum:
List of cloud genomics companies
cloud-genomics
updated 24 days ago by
Jeremy Leipzig
22k • written 10.5 years ago by
14134125465346445
★ 3.6k
26
votes
12
replies
7.5k
views
6 follow
Forum:
Mapping God Found ‘Scientifically Dishonest’ By Anonymous Peer Reviewers
ngs
updated 12 months ago by
zyan2005
▴ 10 • written 10.5 years ago by
johnblue81
▴ 50
0
votes
2
replies
780
views
Forum:
How to start in bioinformatics?
career
updated 11 months ago by
Ram
43k • written 12 months ago by
caffrey
• 0
4
votes
5
replies
1.4k
views
Forum:
I'm looking for a mentor in Bioinformatics
mentor
teaching
updated 11 weeks ago by
alva
• 0 • written 12 months ago by
cwaingartentrabajo
• 0
10
votes
15
replies
2.1k
views
Forum:
How should reference genome fasta files be distributed by UCSC?
reference-genome
fasta
freeze
ucsc-genome-browser
updated 11 months ago by
Jeremy Leipzig
22k • written 14 months ago by
Maximilian Haeussler
★ 1.6k
5
votes
4
replies
1.3k
views
Forum:
Best BAM viewer in 2022
sam
bam
updated 10 months ago by
Ram
43k • written 17 months ago by
Cornelius
▴ 70
1.2k
votes
206
replies
59k
views
108 follow
Forum:
What Are The Most Common Stupid Mistakes In Bioinformatics?
software
updated 11 months ago by
Raony Guimarães
★ 1.4k • written 13.1 years ago by
Jeremy Leipzig
22k
15
votes
13
replies
4.1k
views
8 follow
Forum:
Does a PhD or work experience set you up better to do bioinformatics work in industry?
advice
industry
phd
updated 3 months ago by
benformatics
3.9k • written 17 months ago by
buhbs
▴ 30
4
votes
5
replies
1.4k
views
6 follow
Forum:
Which Bioinformatics Master's degree is better?
career
masters
updated 11 months ago by
Ram
43k • written 18 months ago by
stelakarayani7
• 0
3
votes
6
replies
1.1k
views
Forum:
How to become a moderator and other ways of contributing to Biostars
meta
biostars
updated 11 months ago by
Ram
43k • written 19 months ago by
iraun
6.2k
3
votes
2
replies
852
views
Forum:
From protein-protein interaction networks to..
workflow
computational
knowledge
limitations
updated 10 months ago by
Ram
43k • written 22 months ago by
BioInfoBeginner
▴ 50
15
votes
11
replies
4.7k
views
9 follow
Forum:
Methodological problems are extremely common for enrichment analysis - beware the pitfalls before you publish
GSEA
DAVID
enrichment-analysis
-geneontology
updated 11 months ago by
Ram
43k • written 2.1 years ago by
mark.ziemann
★ 1.9k
0
votes
3
replies
931
views
Forum:
Project Topics on bioinformatic
Data-analysis
Coding
Molecular-biology
updated 11 months ago by
Ram
43k • written 2.2 years ago by
Abdullahi
• 0
3
votes
1
reply
925
views
Forum:
How to download multiple .gz files from Ensembl Protists database
wget
protist
ensembl
updated 10 weeks ago by
BioinfGuru
★ 1.7k • written 2.2 years ago by
rohitsatyam102
▴ 850
17
votes
14
replies
3.3k
views
Forum:
Asking for feedback on a Python library for computing alignments
alignment
Python
updated 10 months ago by
Alexander
▴ 220 • written 2.3 years ago by
bernhard.liebl
▴ 60
0
votes
1
reply
773
views
Forum:
What are the most important papers/methods in Genomics/Bioinformatics of 2021?
publications
updated 10 months ago by
Ram
43k • written 2.3 years ago by
carlopecoraro2
★ 2.5k
12
votes
7
replies
7.1k
views
Forum:
Differences between SMART-seq2, SMART-seq3, and 10x
cell
smartseq
UMIs
single
10x
updated 5 weeks ago by
dsull
★ 5.9k • written 2.7 years ago by
hamarillo
▴ 70
0
votes
0
replies
520
views
Forum:
Poll about a structured description of a tool, a pipeline
survey
pipeline
updated 10 months ago by
Ram
43k • written 2.8 years ago by
Anastasiya M
• 0
7
votes
20
replies
2.1k
views
Forum:
NGS RNA-Seq Analysis Pipeline Tophat vs STAR
STAR
RNA-Seq
TopHat
updated 11 months ago by
Ram
43k • written 3.3 years ago by
mikefeixu
▴ 10
11
votes
9
replies
2.4k
views
7 follow
Forum:
Feature Selection and Dimensionality Reduction in 10X scRNA-seq data
software-error
pca
scRNA-seq
updated 11 months ago by
Ram
43k • written 4.3 years ago by
ATpoint
82k
0
votes
0
replies
701
views
Forum:
where to get the site mutation rate of SARS-CoV-2?
sequence
sequencing
updated 11 months ago by
Ram
43k • written 3.4 years ago by
2001linana
▴ 40
6
votes
5
replies
1.7k
views
Forum:
Very generally speaking, what are valid criteria to decide that a sample is an outlier?
outliers
updated 11 months ago by
Ram
43k • written 3.3 years ago by
Aspire
▴ 330
7
votes
4
replies
1.2k
views
Forum:
Specific question on torturing the data until it confesses
ipa
gene-set-enrichment
statistics
updated 11 months ago by
Ram
43k • written 3.4 years ago by
Aspire
▴ 330
7
votes
3
replies
1.3k
views
Forum:
Is it still recommended to avoid macOS Big Sur?
mac
updated 11 months ago by
Ram
43k • written 3.4 years ago by
jrleary
▴ 210
460 results • Page
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C: Print Differentially Expressed Exons From Dexseq Results
Answer: Please Explain Window Size and Step Size
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Answer: How do I access genome coverage using SPADES?
fGSEA result to csv
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Recent Replies
Comment: High Malat-1 expression in single cell data
by
dsull
★ 5.9k
I briefly looked at those genes on a genome browser -- there are a lot of repeat elements in those genes, meaning a lot of counts assigned …
Comment: High Malat-1 expression in single cell data
by
carolofharvest
▴ 30
Thank you for sharing. What are your thoughts on the genes Gm42418, AY036118, and Gm26917? Some articles suggest that clusters with an abu…
Comment: Do I need to go back and filter my long-reads?
by
GenoMax
142k
> I think I will filter the reads ultimately. If your data was not run and basecalled using the same exact version of pore/software for a…
Comment: Raw counts using stringtie
by
Ram
43k
What is `stringie.sh`? Try `./stringtie.sh -h` and then `more stringtie.sh`.
Comment: Do I need to go back and filter my long-reads?
by
Ram
43k
> I think you should have the right to delete a question you yourself have submitted If you were to talk to someone, can you make them for…
Comment: Help with choosing a model species for Augustus for a de novo assembled genome.
by
dthorbur
★ 1.9k
I don't have any experience using `BRAKER2` so I cannot comment, but I would be extra careful about data you find on non-model organisms. T…
Comment: Differential Expression Analysis using DESeq2 in R
by
GenoMax
142k
You can copy and paste your code and then use `101010` button in the edit window to format it as text to keep it monospaced. For example …
Comment: Same sequencing sample in multiple lanes. How to analyse it?
by
ST
• 0
I guess this could really depend on the "counting" strategy, the library complexity and possible lane biases. If e.g., EM is used to guide…
Comment: Help with choosing a model species for Augustus for a de novo assembled genome.
by
Vijith
▴ 30
Hi dthorbur, thanks for the response. I have run Augustus using Arabidopsis as a species. I would like to know about the suitability of usi…
Comment: How does gene length effect the number of reads mapped
by
dsull
★ 5.9k
Alicia Oshlack has done some cool work on this but even the plots in her papers show that TPMs sometimes overcorrect and the relationship i…
Comment: Differential Expression Analysis using DESeq2 in R
by
Erina
• 0
![The below image contains the code and result also.][1] [1]: /media/images/96562b96-d508-4f67-96d1-7c71aa17
Comment: High Malat-1 expression in single cell data
by
dsull
★ 5.9k
My response above was actually partly inspired off of a reading of your paper :)
Comment: Differential Expression Analysis using DESeq2 in R
by
ATpoint
82k
> everything is showing equal in environment section. But when I am checking for equalities, it’s showing False No idea what this means. P…
Answer: Analysis of intronic reads included scRNA-seq data
by
t.montserrat.ayuso
▴ 10
You can calculate the percentage of intronic reads for each cell using the [DropletQC][1] package for R. In this preprint we discuss th…
Comment: How does gene length effect the number of reads mapped
by
i.sudbery
19k
I did say almost - obviously things are going to get tricky at gene ends - this is why we have the concept of effective length. Its interst…
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