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3
votes
3
replies
960
views
Forum: Importance of Data Structures for Bioinformatics?
data-structures
updated 8 months ago by 44k • written 8 months ago by • 0
19
votes
15
replies
2.3k
views
Forum: What is the amount of sequencing data produced annually?
data research sequencing
updated 1 day ago by • 0 • written 8 months ago by ▴ 70
19
votes
10
replies
1.6k
views
Forum: Pain points using commercial clouds
gpt tinybio
updated 8 months ago by ▴ 70 • written 8 months ago by ▴ 850
9
votes
6
replies
2.0k
views
6 follow
Forum: GTF files from Ensembl Releases 105 and 106 unsorted
Ensembl bug GTF
updated 8 months ago by 82k • written 18 months ago by ▴ 30
3
votes
2
replies
653
views
Forum: How to contribute to the next edition of Biostars Handbook
handbook
updated 8 months ago by 44k • written 8 months ago by ▴ 20
3
votes
6
replies
1.5k
views
6 follow
Forum: Is Bioinformatics a Professional Degree?
career degree
8 months ago by anasjamshed ▴ 120
2
votes
5
replies
982
views
6 follow
Forum: Biostar as a next generation version
meta mobile-app
updated 7 months ago by 44k • written 8 months ago by • 0
2
votes
9
replies
1.2k
views
Forum: open source projects looking for contributors
open-source
updated 8 months ago by ▴ 70 • written 8 months ago by ▴ 20
0
votes
0
replies
553
views
Forum: Recruiters: what do you expect on a junior bioinformatician?
career skills
updated 9 months ago by 44k • written 9 months ago by ▴ 20
3
votes
4
replies
1.4k
views
Forum: Discussion: Uploading terabytes of data to NCBI SRA
NCBI WGS archiving servers remote
updated 9 months ago by 142k • written 9 months ago by ▴ 130
0
votes
0
replies
591
views
Forum: I have a Master's and little experience in Bioinformatics - what would be the next best step?
career
updated 9 months ago by 44k • written 9 months ago by ▴ 10
1
vote
3
replies
807
views
Forum: Spaceranger missing spatial coordinates [Discussion]
Visium
updated 9 months ago by 142k • written 9 months ago by ▴ 550
11
votes
8
replies
1.5k
views
6 follow
Forum: How to write a review article in bioinformatics
article review
updated 8 months ago by 44k • written 9 months ago by ▴ 10
365
votes
58
replies
70k
views
44 follow
Forum: Recommend Your Favorite Bioinformatics Books
education books
updated 7 months ago by 44k • written 14.2 years ago by 100k
1
vote
2
replies
18k
views
Forum: Would you rather analyze a dataset with a strong, non-collinear batch effect, or a weak, collinear batch effect.
collinear latent batch-effect variable GLM
10 months ago by LauferVA 4.2k
0
votes
3
replies
3.9k
views
10 follow
Forum: Discussion question: Cutoff for SNP phylogeny
Phylogenetics SNP
written 17 months ago by ▴ 120
2
votes
3
replies
824
views
Forum: Purely analytic or in silico manuscripts that have significantly improved biological understanding
dry-lab
updated 10 months ago by 142k • written 10 months ago by 4.2k
0
votes
2
replies
1.3k
views
Forum: [Detailed Question] How to Normalize of Metagenomics Gene Abundances (with HMMs models)
normalization
updated 11 months ago by 10k • written 11 months ago by • 0
33
votes
23
replies
4.3k
views
8 follow
Forum: Is there a file format better suited for the era of pangenomics than the .vcf? What are its attributes?
VCF file-format genomics
updated 11 months ago by 22k • written 11 months ago by 4.2k
7
votes
8
replies
2.0k
views
Forum: Would solving many Rosalind problems show your ability for this field in job-wise?
rosalind career
11 months ago by K • 0
2
votes
3
replies
889
views
Forum: Mapping a predicted sequence to a genome
predicted map alignment sequence
updated 11 months ago by ★ 27k • written 11 months ago by • 0
0
votes
0
replies
460
views
Forum: Recommendation sought for differential exon usage of a single gene
splicing differential-exon-usage
updated 11 months ago by 44k • written 11 months ago by ▴ 10
7
votes
4
replies
1.1k
views
Forum: [Meta] Use of AI
AI Artificial-Intelligence meta ChatGPT
updated 11 months ago by 44k • written 11 months ago by 4.2k
2
votes
2
replies
623
views
Forum: Colleges
Colleges india universities
updated 11 months ago by 44k • written 11 months ago by • 0
19
votes
15
replies
2.1k
views
6 follow
Forum: Cross out line in Biostars
meta
updated 12 months ago by 44k • written 6.0 years ago by 5.3k
121
votes
33
replies
39k
views
24 follow
Forum: Where To Look For Quality Bioinformatics Short Courses And Workshops?
Courses
updated 12 months ago by ★ 2.5k • written 10.7 years ago by 11k
103
votes
42
replies
31k
views
34 follow
Forum: List of cloud genomics companies
cloud-genomics
updated 6 weeks ago by 22k • written 10.5 years ago by ★ 3.6k
26
votes
12
replies
7.5k
views
6 follow
Forum: Mapping God Found ‘Scientifically Dishonest’ By Anonymous Peer Reviewers
ngs
updated 12 months ago by ▴ 10 • written 10.5 years ago by ▴ 50
0
votes
2
replies
793
views
Forum: How to start in bioinformatics?
career
updated 12 months ago by 44k • written 13 months ago by • 0
4
votes
5
replies
1.4k
views
Forum: I'm looking for a mentor in Bioinformatics
mentor teaching
updated 3 months ago by • 0 • written 13 months ago by • 0
12
votes
5
replies
2.1k
views
6 follow
Forum: Bioinformatics opportunities in Europe?
career
updated 13 months ago by ▴ 40 • written 13 months ago by • 0
19
votes
14
replies
2.1k
views
7 follow
Forum: What's the general view on point & click bioinformatics for routine analyses?
ngs RNA-Seq next-gen-sequencing
13 months ago by Simon ▴ 40
0
votes
0
replies
7.0k
views
Forum: Optimal approach for variant calling with 3 diluted germline sample in one sequencing - Capture Sequencing
germline Calling Variant Capture Sequencing
13 months ago by Alexandre Eeckhoutte • 0
11
votes
11
replies
1.9k
views
7 follow
Forum: Any tips in landing a bioinformatic job?
Spain R
updated 13 months ago by 4.2k • written 13 months ago by ▴ 30
4
votes
2
replies
718
views
Forum: advice on course selection for a bioinformatics career.
career
updated 14 months ago by 100k • written 14 months ago by • 0
0
votes
1
reply
552
views
Forum: Graduate in molecular biology and genetics
biology genetics Molecular
updated 14 months ago by 142k • written 14 months ago by • 0
4
votes
1
reply
805
views
Forum: Open problems in genomics 2023
genomics pangenomics
updated 14 months ago by 142k • written 14 months ago by 6.4k
5
votes
3
replies
965
views
Forum: Someone posted the same question to StackOverflow and Biostars. The results will blow your mind.
meta
updated 14 months ago by 21k • written 14 months ago by ▴ 70
1
vote
0
replies
488
views
Forum: Penetrance versus heritability
penetrance heritability
14 months ago by tacrolimus ▴ 140
10
votes
15
replies
2.2k
views
Forum: How should reference genome fasta files be distributed by UCSC?
reference-genome fasta freeze ucsc-genome-browser
updated 11 months ago by 22k • written 15 months ago by ★ 1.6k
8
votes
4
replies
1.0k
views
Forum: Implementing FAIR data principles to a bioinformatics lab
data
updated 15 months ago by 22k • written 15 months ago by ★ 1.5k
0
votes
0
replies
472
views
Forum: NGSrich - hg38
Coverage NGSrich
15 months ago by RK • 0
5
votes
5
replies
2.3k
views
Forum: Bioinformatics - how to start?
molecular-biology
updated 15 months ago by ★ 27k • written 15 months ago by • 0
8
votes
8
replies
1.8k
views
7 follow
Forum: A Computer Scientist who wants to start Bioinformatics
computer-science books resources ngs
updated 15 months ago by ▴ 10 • written 17 months ago by • 0
7
votes
3
replies
1.1k
views
Forum: Find a research paper for reproducing the results
research paper
updated 16 months ago by 142k • written 16 months ago by ★ 2.6k
1
vote
1
reply
809
views
Forum: Gene ontology vs Gene set enrichment
set enrichment GSEA ontology Gene analysis gene
updated 17 months ago by ★ 4.3k • written 17 months ago by • 0
6
votes
12
replies
2.3k
views
Forum: How to decide when to have local hardware vs cloud computing?
hardware computational-resources cloud
17 months ago by Lluís R. ★ 1.2k
15
votes
11
replies
2.8k
views
7 follow
Forum: BioStars and ChatGPT
ChatGPT Meta GPT
updated 13 months ago by ▴ 590 • written 17 months ago by ▴ 360
18
votes
10
replies
2.5k
views
7 follow
Forum: Add artificial intelligence training related disclaimers to your profile
licensing AI
updated 17 months ago by 54k • written 17 months ago by 100k
7
votes
2
replies
926
views
Forum: Improvement of Wikipedia articles with bioinformatic subjects
Wikipedia Bioinformatics
16 months ago by Matthias Zepper 4.6k
1,000 results • Page 2 of 20
Recent Votes
C: Classifying normal vs cancer tissues from mutational data
Classifying normal vs cancer tissues from mutational data
Pymol Scripting Help
A: Not sure if pipe from bowtie2 to samtools is working?
Comment: FastQC interpretation - 16S sequencing
Comment: FastQC interpretation - 16S sequencing
Answer: vg autoindex seems stuck after a week of running
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Recent Replies
Comment: FastQC interpretation - 16S sequencing by GenoMax 142k
Since a specific region is being amplified it is expected that many of the library fragments will have identical sequences. Thus the low nu…
Comment: FastQC interpretation - 16S sequencing by Christopher • 0
@genomax Thank you so much for replying. I was quite unsure about the qualities. One more question. There is no problem within nucleotide d…
Comment: FastQC interpretation - 16S sequencing by GenoMax 142k
> My specific question is about the feasibility of per base sequence content analysis and whether I should be concerned about a particular …
Comment: How to get the ratio of allele counts from GATK derived VCF file? by anikcropscience ▴ 230
But I used the same bam files in both freebayes and GATK. Even the overlapping SNPs from both software are giving different estimates in te…
Comment: How to get the ratio of allele counts from GATK derived VCF file? by Pierre Lindenbaum 162k
may be downsampling(?), QC failing reads ?
Comment: How to get the ratio of allele counts from GATK derived VCF file? by anikcropscience ▴ 230
Thank you. However, I extracted the AD for each SNP that gives two numbers separated by a comma. I guess the first one is for reference and…
Answer: How to get the ratio of allele counts from GATK derived VCF file? by Pierre Lindenbaum 162k
> However, I am doing SNP calling using GATK, but there is no such information field for reference and alternate allele counts. it's in …
Comment: samtools write-index by GenoMax 142k
> The index file is older than the data file: alignment.sorted.bam.bai I don't think that warning is going to cause any issues. Not sure w…
Comment: samtools write-index by Pierre Lindenbaum 162k
can you please retry, but add rm -vf alignment.sorted.bam.bai before invoking minimap2. And `-o alignment.sorted.bam` should be enoug…
Answer: from CRAM to fastq by Pierre Lindenbaum 162k
if the CRAM/BAM is paire-end, it **must** be sorted on query name using samtools collate https://x.com/lh3lh3/status/1132756684789768202 …
Comment: from CRAM to fastq by machaalani29 • 0
one of the issues is that I am not very sure how the sequencing was done, but I think it was single end. any suggestions hoot do that?
Comment: from CRAM to fastq by machaalani29 • 0
the option -c is to compress the file
Comment: from CRAM to fastq by GenoMax 142k
Possibly. You appear to have written the data to a single file (in case it was paired-end to begin with). Check to make sure secondary alig…
Comment: converting transcript IDs (Ensembl) to gene symbols in R by GenoMax 142k
https://www.biostars.org/u/18990/ : Please do not delete threads that have received a comment/answer.
Comment: downloading chemical database from ChEMBL by GenoMax 142k
Activities with `cucurbitacin A`: https://www.ebi.ac.uk/chembl/web_components/explore/activities/STATE_ID:RHxG8uDfrtbmiyaY55ic-w%3D%3D `cu…
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