Are you planning to submit this, I'm assuming newly sequenced genome, to one of the big databases (e.g. NCBI)? If so then you should definitely ask to get access to their annotation tools [?]here[?]

Otherwise, there are quite a few tools out there. Here are some of them:

http://molbiol-tools.ca/Promoters.htm

http://linux1.softberry.com/berry.phtml (specifically [?]BPROM[?])

[?]BacPP is another one[?]

Hope this helps