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How to extract the mismatched unique region from Mauve ?

Dear all, Now, i am working in the field of comparative genomics, and am using mauvealigner to find the unique regions of my bacterial draft genomes, example : Genomic islands . I had aligned my draft genome against with the suitable reference genome and i got some mismatched unique regions in my draft. Now i want to extract that specific unique regions in the .fasta format from mauve. How i can do that, need your suggestions. Thanks in advance.

K.Kathir

assembly genome next-gen sequencing sequence

1 answer

In past, I have used following script to convert xmfa to fasta. https://github.com/lskatz/lskScripts/blob/master/convertAlignment.pl

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