Latest
Open
Jobs
Tutorials
Tags
About
FAQ
Community
Planet
New Post
Log In
New Post
Latest
Open
News
Jobs
Tutorials
Forum
Tags
Planet
Users
Log In
Sign Up
About
Limit : all time
all time
today
this week
this month
this year
116,738 results • Page
4 of 2335
Sort: Rank
Rank
Views
Votes
Replies
3
votes
3
replies
305
views
genome assembly records not present in assembly_summary.txt
ncbi
bacteria
assembly
updated 7 days ago by
GenoMax
142k • written 7 days ago by
sapuizait
▴ 10
1
vote
0
replies
158
views
Simulation of label-free bottom-up proteomics expression dataset
label-free
bottom-up
lc-ms
proteomics
7 days ago by
KABILAN
▴ 70
0
votes
0
replies
176
views
Running Phylogenetic Analysis With NCBI Genome
population-genetics
phylogenetic
updated 6 days ago by
Ram
43k • written 7 days ago by
SineWave
• 0
0
votes
0
replies
172
views
RNA-seq: full length gene
RNA-seq
updated 6 days ago by
Ram
43k • written 7 days ago by
Nargis
• 0
1
vote
7
replies
492
views
gvcf joint calling
WES
GATK
VCF
gVCF
5 days ago by
zihanss
• 0
0
votes
0
replies
154
views
Haplotype Phased Assembly Contigs to Chromosome Annotations
Assembly
phased
Haplotype
Annotation
7 days ago by
turcoa1
• 0
3
votes
3
replies
303
views
Sequence read length shorter than flow cell specification
illumina
NGS
sequencing
updated 7 days ago by
swbarnes2
14k • written 7 days ago by
M
• 0
0
votes
0
replies
165
views
Designing single-stable RNA molecules
structure
RNA
7 days ago by
Edna
• 0
0
votes
0
replies
175
views
How to visualize/predict the final transcript from Delly output?
WGS
DELLY
7 days ago by
simplitia
▴ 130
1
vote
1
reply
228
views
Normalize scRNAseq data to housekeeping genes to compare several datasets
RNA-sequencing
housekeeping
Single-cell
normalization
updated 7 days ago by
ATpoint
82k • written 7 days ago by
AaronJaime
• 0
0
votes
2
replies
294
views
BLAST using both nucleotides and taxonomic local databases
blast
ncbi
taxid
taxonomy
updated 7 days ago by
GenoMax
142k • written 8 days ago by
Begonia_pavonina
▴ 150
0
votes
3
replies
381
views
why renaming Idents in Seurat object doesn't work?
Seurat
RenameIdents
R
2 days ago by
Assa Yeroslaviz
★ 1.8k
1
vote
2
replies
333
views
ScRNAseq-How to correctly choose cell type marker genes
cellAssign
cell-markers
5 days ago by
Francesco
▴ 10
1
vote
7
replies
488
views
ScRNA data question
scRNA
Vlnplot
Samples
updated 3 days ago by
bk11
★ 2.4k • written 8 days ago by
starswillfade
▴ 10
0
votes
0
replies
164
views
News:
Master Meta-analysis with R (May 13-16) - Online!
Meta-Analysis
Statistics
R
updated 7 days ago by
Ram
43k • written 7 days ago by
carlopecoraro2
★ 2.5k
0
votes
0
replies
160
views
How to calculate correlation coefficient for chipseq?
chipseq
bigwigsummary
correlation
7 days ago by
Emily
▴ 10
1
vote
0
replies
171
views
How to calculate reliable Ka/Ks or dN/dS ratio for genes of interest from VCF file
dnds
kaks
VCF
7 days ago by
rohitsatyam102
▴ 850
0
votes
0
replies
155
views
how to read graph_test output of monocle 3
monocle3
7 days ago by
synat.keam
▴ 100
1
vote
0
replies
206
views
Job:
CTO/founding engineer role at Voyant Bio
engineer
7 days ago by
Assaf
▴ 10
0
votes
1
reply
499
views
GAPIT p-value significance threshold
GAPIT
p-value
GWAS
updated 7 days ago by
ginellegrenier
• 0 • written 5 months ago by
Clayton
• 0
3
votes
4
replies
348
views
Why gatk VariantAnnotator required bam and coverage files
gatk
VariantAnnotator
7 days ago by
QX
• 0
2
votes
1
reply
238
views
Finding orthologs between genome error
OMA
OMAbrowser
updated 8 days ago by
Adrian Altenhoff
★ 1.1k • written 8 days ago by
hemantcnaik
• 0
0
votes
0
replies
153
views
Why not use iBAQ for calculating differential abundance of proteins?
protein
maxquant
8 days ago by
Aspire
▴ 330
2
votes
5
replies
350
views
Figures are disappeared from html report of SnpEff
HTML
SnpEff
4 days ago by
analyst
▴ 30
0
votes
3
replies
301
views
Highest variable features in single cell data
single-cell
updated 6 days ago by
bk11
★ 2.4k • written 8 days ago by
carolofharvest
▴ 30
0
votes
1
reply
222
views
Network Alignment
plugin
Cytoscape
CytoMCS
updated 8 days ago by
Scooter
▴ 280 • written 9 days ago by
Akash D
▴ 40
2
votes
4
replies
2.4k
views
How could I generate a gi_taxid_nucl.dmp file similar to the one previously hosted by NCBI?
ncbi
metagenomics
centrifuge
gi_taxid_nucl.dmp
taxonomy
updated 8 days ago by
ZhangYuanfeng
• 0 • written 2.4 years ago by
Frazier Baker
• 0
1
vote
3
replies
377
views
PCA plot
DESeq2
PCAplot
updated 4 days ago by
LauferVA
4.2k • written 8 days ago by
Aaliya
▴ 10
4
votes
4
replies
444
views
Tutorial:
how to combine multiple RNAseq count files into a single dataframe in R and unix
Unix
RNAseq
R
updated 6 days ago by
BioinfGuru
★ 1.7k • written 8 days ago by
Ming Tommy Tang
★ 3.9k
0
votes
4
replies
2.7k
views
Lositan freezing when generating selection table
LOSITAN
updated 8 days ago by
evawillms183
• 0 • written 8.3 years ago by
andrepleao
• 0
0
votes
0
replies
151
views
Can you give me an example of a small globular protein (PDB) that has disordered alpha-carbons?
protein
pdb
alpha-carbon
8 days ago by
4fzcgueyp5
• 0
2
votes
3
replies
334
views
clustalo - align milions of short seq, memory issue
memory
clustalo
updated 10 days ago by
Mensur Dlakic
★ 27k • written 11 days ago by
emmanouil.a
▴ 120
0
votes
2
replies
262
views
Annovar using R package
Annovar
gnomAD
R
7 days ago by
DKA
▴ 40
0
votes
2
replies
238
views
Creating Synthetic Sequences for a ML Model
DNA
ML
updated 8 days ago by
Mensur Dlakic
★ 27k • written 8 days ago by
biochugs
• 0
8
votes
8
replies
628
views
PCA plot (Suggestions Needed)
DESeq2
PCAplot
updated 8 days ago by
swbarnes2
14k • written 9 days ago by
Aaliya
▴ 10
1
vote
8
replies
862
views
Adding CB tag to bam file
samtools
bam
updated 8 days ago by
Pierre Lindenbaum
161k • written 14 days ago by
Maria
• 0
1
vote
10
replies
975
views
Troubleshooting RNA-seq data with DNA contamination
RNA-seq
DESeq2
DNA-contamination
8 days ago by
DKA
▴ 40
0
votes
2
replies
825
views
AGeNT LocatIt
AGeNT
LocatIt
WES
UMI
duplicates
updated 8 days ago by
barslmn
★ 2.1k • written 2.8 years ago by
jhy
▴ 10
0
votes
5
replies
369
views
Is it possible to get a list of representative genomes from a past RefSeq release?
representative
ncbi
asembly
refseq
updated 8 days ago by
GenoMax
142k • written 8 days ago by
Bertalan_Takacs
▴ 90
0
votes
0
replies
147
views
One error problem occurred caused by data type during using GDCprepare to handle BRCA data
TCGA
GDCprepare
updated 8 days ago by
Ram
43k • written 8 days ago by
glaciya2018
• 0
0
votes
0
replies
310
views
roary not working with pgap output gff files with fasta sequence but works with prokka's gff outputs
pangenome
PGAP
NCBI
roary
Prokka
9 days ago by
pramach1
▴ 40
0
votes
13
replies
3.6k
views
6 follow
Tax4Fun2 - error
tax4fun
silva
blast
updated 9 days ago by
drstalinantony28
• 0 • written 2.2 years ago by
ymj
▴ 10
0
votes
1
reply
169
views
GEMMA GWAS how to specify factor or numerical for covariates
covariate
GEMMA
GWAS
updated 8 days ago by
Sofia
• 0 • written 9 days ago by
mawigoj318
• 0
0
votes
0
replies
149
views
Sequence BLAST in plus/minus and plus/plus
blast
CRISPR
strand
sequencing
9 days ago by
salias
• 0
4
votes
7
replies
497
views
To get p-values for the TPM
P-value
TPM
RSEM
updated 8 days ago by
dsull
★ 5.9k • written 9 days ago by
VITALA
• 0
0
votes
5
replies
365
views
different FeatureCounts output for the same data
fpkm
Counts
Rsubread
rna-seq
updated 5 days ago by
Istvan Albert
100k • written 9 days ago by
sehriban.buyukkilic
▴ 10
0
votes
0
replies
128
views
Alternative splicing analysis using SUPPA tool - how to normalise counts?
rna-seq
single-cell
suppa
alternative-splicing
updated 8 days ago by
Ram
43k • written 9 days ago by
newuser2024
• 0
1
vote
2
replies
246
views
alignment result
RNA-seq
samtools
hisat2
8 days ago by
ahmad.sajad4541
• 0
0
votes
0
replies
122
views
News:
hands-on introduction to generalized linear models (GLMs) using R
Generalized-Linear-Models
GLM
R
updated 8 days ago by
Ram
43k • written 9 days ago by
carlopecoraro2
★ 2.5k
0
votes
2
replies
235
views
Wilcox test using data slot (log normalized count values) of scRNA-seq between two groups
scRNAseq
9 days ago by
mropri
▴ 150
116,738 results • Page
4 of 2335
Recent Votes
Understanding ATAC-seq data
High Malat-1 expression in single cell data
Answer: DSEQ2 analysis
High Malat-1 expression in single cell data
Convert vcf files with phased genotypes to standard haplotype format
Convert vcf files with phased genotypes to standard haplotype format
A: Convert vcf files with phased genotypes to standard haplotype format
Recent Locations •
All
United States,
just now
China,
just now
Bangladesh,
just now
United States,
just now
United States,
2 minutes ago
Italy,
3 minutes ago
Estonia,
4 minutes ago
Recent Awards •
All
Commentator
to
e.r.zakiev
▴ 210
Scholar
to
dsull
★ 5.9k
Scholar
to
eebloom
▴ 80
Popular Question
to
ManuelDB
▴ 80
Popular Question
to
rustykb
▴ 20
Popular Question
to
sativus
▴ 20
Student
to
Mariana
▴ 40
Recent Replies
Comment: Raw counts using stringtie
by
ahmad.sajad4541
• 0
![stringtie error][1] [1]: /media/images/6475a009-e1a6-412b-8515-a8aec173
Answer: Differential Expression Analysis using DESeq2 in R
by
Erina
• 0
![The below image contains the code and result also.][1] [1]: /media/images/96562b96-d508-4f67-96d1-7c71aa17
Comment: High Malat-1 expression in single cell data
by
dsull
★ 5.9k
My response above was actually partly inspired off of a reading of your paper :)
Comment: Differential Expression Analysis using DESeq2 in R
by
ATpoint
82k
> everything is showing equal in environment section. But when I am checking for equalities, it’s showing False No idea what this means. P…
Answer: Analysis of intronic reads included scRNA-seq data
by
t.montserrat.ayuso
• 0
You can calculate the percentage of intronic reads for each cell using the [DropletQC][1] package for R. In this preprint we discuss th…
Comment: How does gene length effect the number of reads mapped
by
i.sudbery
19k
I did say almost - obviously things are going to get tricky at gene ends - this is why we have the concept of effective length. Its interst…
Answer: High Malat-1 expression in single cell data
by
t.montserrat.ayuso
• 0
Malat1 correlates with the intronic content and can be used as a nuclear indicator. In this preprint we discuss about this artifact and the…
Comment: How does gene length effect the number of reads mapped
by
dsull
★ 5.9k
I wouldn't say it's true that number of reads is linearly proportional to the length of the gene. That's just an assumption that people mak…
Comment: Help with choosing a model species for Augustus for a de novo assembled genome.
by
dthorbur
★ 1.9k
Using any of the species present would likely result in a resonable annotation set. You could use *Arabidopsis* since it likely has the mos…
Comment: Downloading older version of a tool
by
GenoMax
142k
Have you tried conda install <pkg>=<version> so conda install smalt=0.5.8 <!-- junk -->
Comment: Generating mpileup file using samtools
by
Joe
21k
Older versions of software are usually available via distribution tools or the websites. It may require a lot of digging, but its almost ce…
Comment: RNA-seq data for deep learning classification
by
Yuju
• 0
Thank you very much for sharing your advice. Yes, it definitely makes sense that with the use of deep learning, models would learn normalis…
Comment: Generating mpileup file using samtools
by
Ruqaiya
• 0
I didn't use the same tool as in the paper...
Comment: read length in structural variant calling
by
eebloom
▴ 80
Yes good idea. I guess the quality of the SV calls and as a proxy for quality the length and distribution of variants called might be infor…
Comment: Generating mpileup file using samtools
by
Ruqaiya
• 0
I just realised I didn't align my reads with the tools they used and used bowtie2 instead. I can't download the older version that is menti…
Traffic: 2474 users visited in the last hour
Content
Search
Users
Tags
Badges
Help
About
FAQ
Access
RSS
API
Stats
Use of this site constitutes acceptance of our
User Agreement and Privacy Policy
.
Powered by the
version 2.3.6