This is a test version of Biostars. For the public version, visit https://www.biostars.org.
1 vote
2 replies
175 views
Looking for messy GFF3 examples to test a Rust AGAT-compatible gxf2gxf beta
gtf agat genome-annotation gff3
updated by Juke34 949 • | written by Ben 1
0 votes
1 reply
107 views
News: Join the Live Webinar: Programming Biology with Genome-scale AI - 10 August - 3 PM CEST
programming genomics webinar ai
written by Physalia-courses 281
0 votes
3 replies
315 views
News: FINAL & LAST CALL - In-Person Workshop - A Practical Introduction to NGS Data Analysis and Variant Calling (Munich, Germany | September 9–11, 2026)
dna-seq variantcalling rna-seq workshop illumina
written by ecSeq Bioinformatics 9
0 votes
1 reply
126 views
Reading gam files within code
gam vg pangenome
updated by Arup Ghosh 402 • | written by guntul 4
0 votes
1 reply
570 views
Harmonising WGS and SNP array data
data handling array snp wgs
updated by SeqBench 41 • | written by FB1999 0
0 votes
1 reply
694 views
Ambient RNA correction when most of the ambient comes from a specific cell type of interest
seurat single-cell cellbender single-nuclei ambient
updated by SeqBench 41 • | written by txema.heredia 31
0 votes
1 reply
111 views
Housekeeping Genes for normalization or doublet detection in scRNA-seq and Spatial Transcriptomics
scrna-seq xenium spatial-transcriptomics housekeeping-genes normalization
updated by SeqBench 41 • | written by npont 3
1 vote
6 replies
349 views
Tool: igblastr: A User-friendly R Wrapper to IgBLAST
igblast bioconductor r igblastr
updated by Jesse 94 • | written by Hervé 1
0 votes
2 replies
215 views
Bioinformatics Tools for Promoter Analysis
bacteria. pseudomoans promoters. insilico. putida
updated by Dr.Omics Labs 0 • | written by camila.desimone 0
0 votes
5 replies
27K views
Converting .txt into .fasta
sequence
updated by GenoMax 16K • | written by trejomarco6 0
0 votes
1 reply
146 views
Analysing Microarray file
degs microarray
updated by Gordon Smyth 916 • | written by rajdeepboral00 7
0 votes
0 replies
72 views
News: Last seats available – Machine Learning for Multi-Omics Integration (21–23 September)
machinelearning multiomics omicsintegration
written by Physalia-courses 281
0 votes
5 replies
331 views
RNAseq data is not significant with no clear clustering, how do I check for sample outliers?
gsea deg rnaseq
updated by mark.ziemann 199 • | written by Rawan 0
0 votes
0 replies
87 views
News: Single-cell RNA-Seq Analysis (SCRN03) – Live Online Training
scrna-seq single rna-seq cell
updated by GenoMax 16K • | written by coursesinfo 0
3 votes
3 replies
286 views
Tool: A website with science news written by AI
news ai
written by shelkmike 201
0 votes
5 replies
3.2K views
Speed up Seurat FindClusters execution?
seurat
updated by E. 0 • | written by CTLong 14
1 vote
0 replies
94 views
News: Online Course: Introduction to Deep Learning for Biologists | 23–27 November
deeplearning ai cnns machinelearning drugdiscovery
written by Physalia-courses 281
0 votes
20 replies
1.2K views
Tool: An automation and orchestration tool - Need honest feedback
orchestration automation
written by Nenad 0
0 votes
11 replies
707 views
Tool: Public NCBI messy and incomplete metadata problem tool
metadata curation messy public ncbi
written by Vy 0
0 votes
2 replies
211 views
Tool: New tool for narrowing difficult missense variant shortlists
software variant-annotation comparative-genomics missense
written by Lara Finley 0
0 votes
0 replies
151 views
Tutorial: New educational resource: Visualizing CNVs and structural variants in IGV
igv ngs cnv
written by wakaruszka 5
2 votes
0 replies
116 views
News: Single Cell & RNA-seq analysis/interpretation course & certification- Become an expert
rnaseq singlecell ngs bioinformatics
written by Nicole 12
2 votes
3 replies
274 views
igv beginer question
alignment
updated by ATpoint 9.1K • | written by jolie 2
1 vote
3 replies
544 views
Looking for some data visualization resources!
data-visualisation genomics igv
updated by wakaruszka 5 • | written by sameer 4
0 votes
8 replies
1.4K views
Analyzing data of Sanger sequencing
sangersequencing
updated by SeqBench 41 • | written by priyankasolanki 0
0 votes
1 reply
125 views
How do I merge Framingham Heart Study phenotype data from BioLINCC with genotype data from dbGaP using participant IDs?
biolincc framingham dbgap genotype phenotype
updated by SeqBench 41 • | written by Krishalls 0
2 votes
1 reply
136 views
Appropriate Hardy-Weinberg Exact Test Alternative for Detecting Candidate Lethal Alleles with Missing Homozygotes?
r snp statistics hardy-weinberg population-genetics
updated by SeqBench 41 • | written by jhonatan.henrique 4
0 votes
1 reply
142 views
cBioPortal vs UCSC Xena — when to use which, and what are the actual pros/cons?
cbioportal tcga r xenabrownser
updated by SeqBench 41 • | written by Kelly 2
0 votes
1 reply
128 views
How should recombinant haplotypes from vg haplotypes personalized GBZ be exported as FASTA?
vg
updated by Jouni Sirén 85 • | written by Etenesh 0
2 votes
3 replies
2.3K views
Forum: Cataloguing available spatial transcriptomics tools, then linking them together into a workflow?
cytassist transcriptomics nanostring xenium visium
written by LauferVA 497
0 votes
4 replies
325 views
De novo assembly for phylogenetic analysis
phylogenetics genomics
updated by shelkmike 201 • | written by isag 0
0 votes
0 replies
81 views
News: Causal AI Methods for Computational Biology – Online Course | 2–6 November
causalinference computationalbiology machinelearning ai
written by Physalia-courses 281
0 votes
9 replies
664 views
News: I wrote a beginner-friendly book on AI in Biology, would love this community's thoughts
book biology ai
written by aadil 5
0 votes
0 replies
105 views
Tool: BIOSZEN: an R/Shiny package for Excel-based data analysis and visualization
data-visualization growth-curves data-analysis statistics microbiology
written by Pinkus 0
0 votes
1 reply
802 views
Purpose and necessity of removing PCR duplicates in m6A-seq analysis
pcr merip-seq m6a-seq duplicates
updated by SeqBench 41 • | written by remagen 1
2 votes
4 replies
1.6K views
PacBio amplicon reads partially aligned using minimap2 – library or analysis issue?
pacbio minimap2 alignment variants pbmm2
updated by SeqBench 41 • | written by louisflower1999 2
0 votes
4 replies
967 views
Convert Sanger FASTA for Y-STR marker into block-by-block repeat counts (motif structure), not just total repeats
y-str sanger fasta
updated by SeqBench 41 • | written by sue 0
0 votes
1 reply
392 views
Cannot trim primers in R2 reads no matter what orientation of primers I use.
cutadapt
updated by SeqBench 41 • | written by DNAngel 26
1 vote
2 replies
911 views
Download metabolic information from databases using the NCBI Taxonomy ID
metabolism database r taxonomy ncbi
updated by Giulio 0 • | written by ThaiosX195 1
0 votes
0 replies
81 views
How should I select inner and outer radii for dual-scale spatial niche analysis of Xenium tumor data?
xenium
written by zhentao 0
0 votes
1 reply
152 views
multiBigwigSummary
wig bigwig multibigwigsummary
updated by Arup Ghosh 402 • | written by sk_24 0
0 votes
0 replies
161 views
Job: Sequencing Job Announcement - Forensic Science
ngs sequencing vacancy forensic job
written by Michael 0
0 votes
0 replies
39 views
News: Online live course: Putting protein structure into phylogenetic inference
phylogenetics protein
written by soledad.esteban 1
2 votes
1 reply
102 views
Differential expression in pseudobulk snRNA-seq with non-independent pseudobulks
rna-seq deseq2 single-cell expression differential
updated by GenoMax 16K • | written by gttg 2
0 votes
1 reply
172 views
Transcript to gene file for Kallisto in a iso-seq transcriptome with no reference genome?
iso-seq kallisto
updated by SeqBench 41 • | written by bptrx 0
0 votes
1 reply
144 views
PFAM double entry for the same PFAM accession with overlapping amino acids, how to keep only one?
interpro domains pfam functional
updated by SeqBench 41 • | written by ieie 2
0 votes
3 replies
258 views
High chrY call rates for human female 30x WGS samples, what am I doing wrong?
gatk plink chromosomes sex callrate
updated by SeqBench 41 • | written by Joel Wallenius 22
0 votes
3 replies
191 views
mob_suite doesn't recognize a plasmid I know is in the sequence
plasmid mob_suite
updated by SeqBench 41 • | written by blur 30
0 votes
3 replies
1.0K views
NTC amplification close to expected amplicon size during primer validation – primer-dimer or non-specific product?
ntc primer-design qpcr pcr
updated by SeqBench 41 • | written by Sashinika 0
0 votes
0 replies
50 views
News: online course: Machine Learning for Computational Cancer Genomics
machinelearning cancergenomics precisionmedicine
written by Physalia-courses 281
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