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0 votes
8 replies
1.9K views
Huge decrease of BUS records using bustools
single-cell bustools 10x
written by julien.wollbrett 0
0 votes
3 replies
1.3K views
Kmergenie install bug
kmergenie
written by joshua.j.wright 1
1 vote
3 replies
3.6K views
How to deal with gaps during translation with biopython
sequence
updated by fournier.berlin 1 • | written by sunyeping 11
0 votes
1 reply
800 views
NCBIminer is not available
ncbiminer
updated by Joe 2.2K • | written by strive 0
0 votes
1 reply
595 views
OpenCravat syntax error during annotation
opencravat
updated by Ram 4.5K • | written by Monika 0
0 votes
2 replies
967 views
annotating single-cell clusters from gene lists
annotation findallmarkers seurat cell-type
updated by Ram 4.5K • | written by Assa Yeroslaviz 197
0 votes
5 replies
3.6K views
Any ways of extracting and filtering read sequences from cram files with samtools?
samtools cram reads sequence
written by sacryt 0
0 votes
0 replies
720 views
Rosalind problem sum: Mendel's First Law
rosalind
updated by GenoMax 16K • | written by Nikita 0
0 votes
4 replies
1.3K views
PCA Plot in Single-cell RNA-seq Data
rna-seq pca single cell
written by Darya 0
0 votes
2 replies
1.7K views
calculate the heterozygosity of an assembly
plant assembly genome genome heterozygosity
updated by Corentin 66 • | written by san96 19
0 votes
0 replies
694 views
How to calculate the percentage of hydrophobic amino acids?
genome rna-seq splicing sequencing
written by Apex92 32
0 votes
1 reply
807 views
Is there any database for transposable elements in cactus plants
edta transposable-elements
updated by Ram 4.5K • | written by rj.rezwan 2
0 votes
0 replies
785 views
RNA-seq GSEA cutoff
gsea
written by maplewj 2
1 vote
7 replies
2.7K views
NGS tertiary analysis
classification variant-annotation
updated by bharata1803 58 • | written by n.awad 2
2 votes
0 replies
1.8K views
Tutorial: Premade library preparation – considerations, tips and tricks (II)
illumina. premade-library. novaseqx.
written by Novogene 54
0 votes
2 replies
1.4K views
Visualize and access gvcf/vcf files
genomics vcf
updated by venkat 1 • | written by QX 9
0 votes
0 replies
654 views
filterBam (Augustus) results explanation
genomeannotation rnaseq annotation filterbam augustus
updated by Kinoppy 0 • | written by Sanam 0
0 votes
2 replies
1.2K views
Why does Phred+ encoding changes after trimming fastq files??
fastqc fastq phred quality trimming
updated by GenoMax 16K • | written by Bikal 0
0 votes
0 replies
908 views
xmlrpc.client.ProtocolError while deeptools bigwigAverage
deeptools
written by Aki 2
0 votes
0 replies
838 views
Exploring CRUD Operations with Mongoose and MongoDB in Bioinformatics
mongodb mongoose
updated by Ram 4.5K • | written by zainabi8077 2
0 votes
0 replies
700 views
News: Online course: Reproducibility in Bioinformatics
docker singularity workflowmanagementsystems reproducibility
written by Physalia-courses 281
0 votes
3 replies
1.8K views
CNV benchmarking: Truvari & HG002 SVs v0.6 (GIAB)
benchmarking truvari cnv
updated by DBScan 53 • | written by emma.a 13
0 votes
1 reply
1.3K views
ENA fastq file shown as Paired-end but has 2 Run Accessions
ena end paired
updated by GenoMax 16K • | written by Aaliya 0
0 votes
0 replies
1.0K views
microbiomeMarker installation
install microbiomemarker docker
written by fabiomarcelomedvet 1
1 vote
0 replies
704 views
Tool: HFKReads, an ultra-fast and efficient tool for high frequency kmer sequencing reads extraction
kmer organelle metagenomic assembly
written by Huiyang 20
0 votes
6 replies
3.6K views
How to interpret the output from a DESeq2 WALD test analysis in R to compare gene expression in two different tissues.
wald gene-expression-comparison deseq2
written by QuayQ BioMe 0
0 votes
1 reply
1.1K views
Job: AN INTEGRATED IN SILICO AND IN VITRO STUDY ON THE DEVELOPMENT OF A COMPUTATIONAL METHOD FOR THE PREDICTION OF O-GLCNAC MODIFIED PROTEINS
proteomics
updated by Ram 4.5K • | written by Anooshian 0
0 votes
1 reply
874 views
Validate the assembly of unmapped reads by remapping the original reads back to the assembled sequence
unmapped_reads. assembly. paired-end_reads
updated by GenoMax 16K • | written by Sony Nguyen 2
0 votes
2 replies
1.3K views
A question about using HISAT2 without biological replicates
rna-seq hisat2
written by vinayjrao 26
1 vote
0 replies
492 views
How to process Iso-seq subreads in fastq downloaed from NCBI SRA database ?
pacbio
written by 2310691738 1
0 votes
0 replies
612 views
Small portion of contigs have high coverage
metagenomics virus read-mapping
updated by Ram 4.5K • | written by Md Moinuddin 0
1 vote
2 replies
1.3K views
Looking for generative AI applications in bioinformatics
genai chatgpt
updated by Ram 4.5K • | written by a615ebfb 8
0 votes
1 reply
1.1K views
How to map CPTAC digital pathology slides to sample ID and metadata
cptac digital-pathology
updated by Zhenyu Zhang 130 • | written by Jakub 0
0 votes
2 replies
1.9K views
installation of deepTools
deeptools deeptools
updated by GenoMax 16K • | written by Sue 0
0 votes
1 reply
745 views
Geo2R - extremely high foldchange
geo2r
updated by ATpoint 9.1K • | written by Sanjukta 0
2 votes
3 replies
1.8K views
Would a string composed of `#CHROM`, `POS`, `REF` and `ALT` uniquely identify a variant?
vcf
updated by LauferVA 497 • | written by gernophil 15
0 votes
4 replies
1.4K views
merge regions into one bigger region
genomicranges
written by Chironex 5
0 votes
3 replies
1.2K views
featureCounts output file chromosome identifier issue
featurecounts rnaseq trnascriptome wts sequencing
updated by GenoMax 16K • | written by tanbiswas6 1
0 votes
2 replies
1.5K views
command not found: bigwigAverage
deeptools
written by Aki 2
0 votes
0 replies
639 views
CellPhoneDB results
cellphonedb cpdb cell_surface_proteins
updated by GenoMax 16K • | written by piotto 2
0 votes
0 replies
628 views
News: Online course: Genome Assembly Using Oxford Nanopore Sequencing - 4 seats remaining
genomeassembly nanopore longreads
written by Physalia-courses 281
0 votes
6 replies
2.0K views
Multiple unique reads sharing same UMI barcode
umi ngs
updated by i.sudbery 2.3K • | written by ww22runner 6
0 votes
1 reply
961 views
How to find clinical data for TARGET AML
target aml
updated by Zhenyu Zhang 130 • | written by veronique.voisin 0
0 votes
0 replies
729 views
Ran both metabat2 and vamb on assembled metagenome sequences, unsure how to interpret differing results between the two
binning metabat2 vamb taxonomy
written by jway 0
0 votes
4 replies
1.7K views
perplexed regarding the process of gene expression normalision
gene rna-seq expression normalization
written by DS 1
0 votes
1 reply
905 views
Refseq IDs conversion to gene symbol
ensembl orthology symbol refseq gene
updated by GenoMax 16K • | written by rgrindle 0
0 votes
4 replies
1.5K views
How can I get the sequences of the first 20nt of introns and the last 250nt of introns of hg38 genome?
annotation rna
updated by Juke34 949 • | written by xiaoleiusc 14
0 votes
2 replies
924 views
Error Reading gencode.v38.genes.json.gz File in SvAnna CLI Tool
structural-variant svanna
written by pragnapcu 1
0 votes
3 replies
1.7K views
Rename BAM file
bam
updated by Ram 4.5K • | written by 8armed 2
0 votes
1 reply
1.1K views
picard CreateSequenceDictionary errors when trying to make a .dict file
picard dict
updated by colindaven 844 • | written by michael.flower.14 21
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