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Recent Replies
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bioinfo
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Thank you for the explanation. Is it more appropriate then to use the sequence from the illumina documentation or the one from the github?
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Thanks. What determines what constitutes a good distance? I have some targeted panel data and looking at flagstat, almost all reads are pai…
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Illumina indexed adapters share a core sequence at the beginning which is what is indicated in your link. Trimming programs will remove seq…
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Please stop using `bioinformatics` as a tag unless your post is about the field of bioinformatics itself.
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> If we switch to pair-end sequencing with dual-index barcode
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That is not valid GFF format, so that is probably going to be problematic. See the [GFF spec](https://github.com/The-Sequence-Ontology/Spec…
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Hey! I had this issue and I think I can help- could you take a screenshot of the .txt file opened in the notepad?
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Hi! I had a very similar issue and it's likely not at all related to your number of samples but to the format of your mixture file! Ciberso…
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Thank you Ram for the comments! Noted for future posts.
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oh, this is helpful. Thanks.
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paired reads = the experiment was a paired end assay: each fragment was sequenced on 5' and 3', two FASTQ files are generated by the seque…
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